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Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for Mouse Spermidine Synthase SRM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The cells were then incubated in a 1:250 Anti-β-Tubulin mouse monoclonal antibody (Millipore-Sigma, T5293-.2ML)-NGS-PBS solution for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-SUN1 pAb (HPA008346) and mouse anti-β-tubulin mAb (T4026, clone TUB2.1) were also from Sigma-Aldrich. Both of them were used at a dilution of 1:200 for immunofluorescent microscopy and at a dilution of 1:1000∼2000 for western blotting ...
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bioRxiv - Cell Biology 2022Quote: ... for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8, 1:2,500, 05-949) for 6xHIS::NMY-2 detection ...
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bioRxiv - Biophysics 2023Quote: ... The cells were then incubated in a 1:250 Anti-β-Tubulin mouse monoclonal antibody (Millipore-Sigma, T5293-.2ML)-NGS-PBS solution for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... was visualized using a mouse monoclonal anti-phospho-Histone H2A.X (Ser139) antibody at a 1:200 concentration (05-636; Millipore) followed by an Alexa Fluor®647 conjugated donkey anti-mouse polyclonal antibody at a 1:1000 concentration (ab150111 ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody, A3682, Sigma Aldrich). All three blots were developed for 9.2 s after adding SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: ... Coverslips were fixed in 4% PFA and processed as described above using mouse α-FLAG M2 (Sigma; 1:1,000) and rabbit α-GFP (Abcam ...
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bioRxiv - Genomics 2023Quote: The immortalized mouse primary fibroblasts were cultured in Dulbecco’s Modified Eagle medium (DMEM/F-12) supplemented with 10% FBS (Sigma), 1% penicillin–streptomycin (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... samples were incubated with 1µg/mL of secondary antibodies (AlexaFluor647 anti-mouse, ThermoFisher, or Rabbit-Atto647N antirabbit, Sigma-Aldrich) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight with an anti-GFP rabbit antibody (0.5 μg/ml) (Tamamaki et al., 2000) and an anti-Cre recombinase mouse monoclonal antibody (1:1,000; MAB3120, Merck Millipore). After a rinse ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Pathology 2023Quote: Human or mouse lung tissue slides (7 μm) were fixed in 3.7% formalin (Sigma-Aldrich, St. Louis, MA, USA), permeabilized in 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... Histone 2B and chromatin)] and 2° antibody cocktail [1:1000 a-mouse-Cy3 (cat.no. 715-166-150, Amersham, UK) and 10µg/ml Hoechst 33342 (cat.no. 14533, Sigma-Aldrich, USA)] (69–71) ...
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bioRxiv - Neuroscience 2023Quote: The following primary antibodies were used for this study: mouse monoclonal anti synaptophysin (SP) (clone SVP38, 1.4 ug/ml, Sigma), guinea pig polyclonal anti VGlut11 (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... antibodies were purchased from Developmental Studies Hybridoma Bank. Mouse anti β-ACTIN (cat. A5316, RRID: AB_476743; 1:1,000 for IB) was purchased from Sigma. Goat anti-Mouse IgM (Heavy chain ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse zygotes were isolated in FHM under mineral oil and cumulus cells were removed with hyaluronidase (Sigma-Aldrich; H4272). Mouse embryos were cultured in pre-equilibrated Global Media (Cooper Surgical ...
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bioRxiv - Microbiology 2023Quote: ... spleen cells from a single mouse were harvested and stimulated for 6 h with 16 nM PMA (Sigma-Aldrich) and 1 μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit polyclonal anti-Laminin (1:50; Santa Cruzcat # sc20142) and mouse monoclonal anti-CSPG (1:200; Sigma cat # C8035). Phalloidin Alexa Fluor 594–conjugated (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 1:500 dilution of rabbit anti-MeCP2 (Cell signalling) and 1:10000 dilution of mouse anti-vinculin (Sigma Aldrich). After the incubation with the primary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by application of a Cy3-conjugated goat anti-mouse antibody (1:250 dilution, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit IgG horseradish peroxidase (HRP)-linked antibodies (Amersham) and streptavidin HRP-conjugated antibodies (Sigma-Aldrich) were used for ECL detection Reagents (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-5 mm3 biopsies from naked mole-rat or shaved mouse back skin were incubated with either: I) dissociation buffer (1.25 mg/ml collagenase I (Sigma), 0.5 mg/ml collagenase II (Worthington) ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-human-Dia2-isoform1 (gift from Véronique Pizon) and GFP-mouse-Dia2-isoform3 (gift from Yosuke Senju) using X-tremeGENETM HP DNA Transfection Reagent (Sigma) according to the manufacturer’s instructions cultured for a total of 2 days ...
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bioRxiv - Neuroscience 2023Quote: ... They were stained with 1/400 (v/v) of primary antibody mouse anti-rhodopsin clone 4D2 (MABN15, Sigma-Aldrich) and allowed to incubate for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Coverslips with either adult mouse or neonatal rat cardiomyocytes were incubated 1h with anti-α-actinin antibody (A7811, Sigma) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubating with the primary antibody (mouse monoclonal anti-acetylated tubulin, 6-11B-1, 1:1000, Sigma T6793) and then with the secondary antibody (goat anti-mouse IgG-Alexa Fluor 488 ...
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bioRxiv - Cancer Biology 2023Quote: ... in -20°C methanol for 10 minutesCells were stained with mouse anti-γ-tubulin 1:500 (Sigma Aldrich, T5326), rabbit anti-KIF18A 1:100 (Bethyl ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies (insulin rabbit monoclonal, Cell Signaling 3014, 1:3000 and glucagon, mouse monoclonal, Sigma-Aldrich G2654, 1:1000) were applied and incubated for 60 minutes followed by secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... the tissues were incubated with primary mouse anti–α-actinin (sarcomeric) antibody (1:750, Sigma-Aldrich, cat. no. A7811), and vimentin (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed in PBS-T (0.05% Tween-20 in PBS) and PBS before incubation with 1:10,000 dilution of HRP-conjugated anti-mouse IgG (Sigma) for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated in a 1:500 dilution of 4D2 mouse anti-PLSCR1 antibody (Millipore Sigma, cat. #MABS483) in PBS-BG (1x PBS with 0.5% bovine serum albumin and 0.1% glycine ...
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bioRxiv - Developmental Biology 2024Quote: Primary antibodies used in this study include: Phospho Histone H3 Ser10 Mitosis Marker mouse 1:500 (Millipore 06-570), MMP1 catalytic domain mouse 1 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed thrice with TBS-T buffer prior to addition of horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in blocking solution (anti-mouse-HRP 1:10,000 or anti-rabbit-HRP 1:10,000 antibodies; all from Sigma Aldrich) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubating with the secondary antibodies (anti-Mouse MINUS, DU092004, Sigma-Aldrich; anti-Rabbit PLUS, DUO92002, Sigma-Aldrich) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated for 1 hour with the appropriate peroxidase-conjugated anti-mouse secondary antibody at 1/5,000 (EMD Millipore). Immunoreactive proteins were visualized using the LumiGLO chemiluminescent substrate (Cell Signaling).
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bioRxiv - Genetics 2024Quote: Mouse embryonic stem cells (ESCs) were grown on 0.1% gelatin-coated culture dishes in Glasgow Minimum Essential Medium (Sigma) supplemented with 20% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... One hundred microliters of the E16.5 mouse brain lysate were digested with 1 unit of hyaluronidase from Streptomyces hyalurolyticus (Sigma) at 37L for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... with two PBS washes after each incubation: primary antibody solution for 1 hour (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636 ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 12 hr at 4°C in the presence of mouse anti-FLAG (1:1000, Sigma #F1804) and rabbit anti-CD40 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... The blocking solution was then replaced with another consisting of 2 µL mouse anti-NeuN antibody (Merck Millipore, MAB377) and 0.5 µL of rabbit anti-GFAP antibody (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... we use primary anti-acetylated tubulin antibody from mouse (monoclonal 6-11 B-1, T7451 Sigma, 1/1000 dilution) as well as primary anti-tyrosinated tubulin antibody from mouse (monoclonal TUB-1A2 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated in a solution containing mouse monoclonal anti-BrdU antibody (1:250, Sigma, Cat.no. B8434) and rabbit polyclonal anti-Doublecortin (DCX ...
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bioRxiv - Biochemistry 2024Quote: ... anti-p-Histone H2A.X Ser139 (ψH2A.X, mouse monoclonal, 05-636) and pan-ADP-ribose binding reagent (rabbit Fc tag, MABE1016) were obtained from Millipore. anti-DHFR (rabbit polyclonal ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged CCT domains (mouse SPAK 459-556 and human OSR1 433-527) and mouse SPAK 62-556 T243E were expressed in Rosetta (DE3) E.coli (Novagen). Cells were grown at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The following primary antibodies were used: GFP (rabbit, in-house, 1:1,000) and Tubulin (mouse monoclonal, 1:8,000; Sigma-Aldrich). As secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were tested on the Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore, Cat No. MCYTOMAG-70K-PMX) according to the kit protocol as qualified ...
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bioRxiv - Cancer Biology 2024Quote: The following primary antibodies and dilutions were used: mouse-anti-γH2AXSer139 1:1,000 (#05-636, clone JBW301, Merck Millipore) and rabbit-anti-53BP1 1:1,000 (NB#100-304 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 x in TBS-Tween (0.1%) + 2% BSA before incubation in TRITC conjugated anti-Mouse secondary antibody (Sigma) at 1:1000 concentration for 1 hr in the dark ...