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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma F-7524), 1000 U/mL LIF ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was additionally supplemented with 2.5% FCS (Sigma Aldrich) from DIV 9 onwards ...
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bioRxiv - Immunology 2023Quote: ... and 8% fetal calf serum (FCS, Sigma-Aldrich Chemie BV). TK-1 murine T-cell lymphoma cells (CRL-2396 ...
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bioRxiv - Immunology 2023Quote: ... complete media supplemented with 10% (vol/vol) FCS (F0804, Sigma), 1% penicillin-streptomycin (15140122 ...
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bioRxiv - Immunology 2023Quote: ... 70% fetal calf serum (FCS) (Sigma-Aldric, St. Louis, MO) and 20% RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... FCS was replaced with 5% human serum (Sigma-Aldrich, H6914). Hypersalinity (high NaCl ...
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bioRxiv - Immunology 2023Quote: ... U251 glioblastoma cells were purchased from Millipore Sigma and maintained in DMEM supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma, F7524-500ML) and 1% Penicillin-Streptomycin (Pen/Strep ...
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bioRxiv - Biophysics 2023Quote: ... which consisted of fluorinated oil (Fluorinert FC-40, Sigma-Aldrich) and 2% w/w fluorosurfactant (RAN biotechnologies ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich, F7524) and maintained in a humidified incubator at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2024Quote: ... and fetal calf serum (10%, FCS, Sigma-Aldrich-Chemie-BV)) with Geneticin G418 Sulphate (0.6 mg/ml ...
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bioRxiv - Microbiology 2022Quote: For immunofluorescence experiments the panel of HEK293 cells grown on poly-Lysine (Sigma-Aldrich Cat. Nr. P6282) pre-coated (2.5 μg/mL in MQ H2O 30 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-GlutaMAX™ -I (Sigma-Aldrich) supplemented with 10% fetal bovine serum (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... and HEK293 control cells were separated by SDS-PAGE using TruPAGE precast gels (4-12%) (Sigma-Aldrich) or 8% Laemmli SDS-gels ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Spike-transfected CEM NKR-Luc cells were stained with PKH26 kit according the manufacturers protocol (Sigma) prior to performing the ADCC assay ...
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bioRxiv - Biophysics 2020Quote: ... Cells were treated with a spike of 25 nM Tetramethylrhodamine ethyl ester perchlorate (TMRE, Sigma 87917) for 30 minutes before imaging ...
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bioRxiv - Genomics 2022Quote: ... These 16 spike-in oligomers were purchased in two separate batches (Table S1) from Sigma Aldrich and resuspended in 10 mM Tris (pH 8 ...
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bioRxiv - Neuroscience 2019Quote: Adult brain dissection was performed in ice-cold S2 cell culture medium (Schneider’s Insect Medium, Sigma Aldrich, #S0146) and brains were fixed with 4% PFA (v/w ...
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bioRxiv - Cell Biology 2021Quote: WT S2 cells (1.5×106) were plated on coverslips and permeabilized with 10 μg/ml digitonin (Sigma- Aldrich) in KRB for 2h at 26°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The Drosophila S2 cells were maintained in HyClone™ SFX-Insect Cell Culture Media containing 10% FBS (Sigma).
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bioRxiv - Neuroscience 2022Quote: ... Quantitative PCR was performed for the amplification of cDNAs using the appropriate primers (Sigma-Aldrich, see Table S2) and the GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Neuroscience 2019Quote: Adult brains dissection was performed in ice-cold S2 cell culture medium (Schneider’s Insect Medium, Sigma Aldrich, #S0146) and brains were fixed with 4% PFA (v/w ...
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bioRxiv - Genetics 2021Quote: ... Drosophila S2R+ (CVCL_Z831, DGRC Cat # 150), S2 DGRC (CVCL_TZ72, DGRC Cat # 6) cells were grown in M3 media (Sigma) containing Bactopetone ...
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bioRxiv - Developmental Biology 2021Quote: Total mRNA from ds- egfp and ds-hyx-treated S2 cells were extracted using TRI Reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Two independent guide RNAs for ITGA5 (Table S2) from the Brunello sgRNA library (49) were synthesized (Sigma- Aldrich) and cloned in to the pLentiCRISPRv2-blast plasmid (#98293 ...
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bioRxiv - Neuroscience 2023Quote: ... and dissected in cold S2 media on a 9-well glass plate coated with silicone (Sylgard 184, Sigma) using forceps (Dumont #5 ...
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bioRxiv - Microbiology 2020Quote: ... plates supplemented with 3% sheep blood (iTek StPaul MN) and 20 μg/mL neomycin (Sigma St Louis MO) and then grown overnight at 37°C in a 5% CO2 humidified incubator ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with TSA blocking reagent containing peroxidase-conjugated sheep anti-DIG antibody (1:1,000, Sigma, 11207733910) at room temperature for 2 h ...
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bioRxiv - Genomics 2020Quote: ... embryos were incubated overnight at 4 °C with sheep anti-DIG conjugated with POD (Sigma-Aldrich cat #11207733910) with 1:500 working dilution in PBT ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then rinsed in PBSDT and incubated in blocking solution 1× sheep serum (SS, Sigma Cat# S2263) for at least 4 hrs at RT ...
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bioRxiv - Biochemistry 2021Quote: ... The MERS-CoV peptide was labeled with sub-stoichiometric FITC (Sigma-Aldrich, St. Louis, Missouri) in a basic HEPES buffer (pH 8.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... M2 anti-FLAG tag (1:1000, from 1 mg mL−1, Sigma), anti-β-actin (1:5000 ...
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bioRxiv - Biochemistry 2022Quote: ... as a His-tag fusion and Ars2206-531 into pRSFduet vector (Novagen) as a Strep-tag fusion ...
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bioRxiv - Microbiology 2021Quote: ... The C-terminal twin-Strep-Tag was removed by HRV3C (Sigma Aldrich) digestion overnight at 4 °C and the uncleaved protein was removed by passage over Ni-NTA resin ...
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bioRxiv - Microbiology 2020Quote: ... Immunoblots were performed with anti-GFP tag primary monoclonal antibody (Sigma-Aldrich) as described by50.
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bioRxiv - Microbiology 2021Quote: ... and the antibody for the FLAG tag was from Sigma (cat # F7425).
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bioRxiv - Immunology 2022Quote: 30µL/well of rabbit anti-His tag antibody (Sigma Aldrich, catalog #SAB5600227) was adsorbed at a concentration of 0.5 μg/mL on to 384 well microplates (Thermofisher ...
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bioRxiv - Plant Biology 2021Quote: ... or western blot using HA Tag Antibodies (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Molecular Biology 2022Quote: ... HA tag antibody (1:1200, cat# 04-902; EMD Millipore, clone DW2). Blots incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into PET-24a (+) C-terminal-histidine-tag vector (Novagen, Germany), which was digested by XhoI and NdeI and dephosphorylated by Antarctic Phosphatase (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... but with removal of the His6 tag was by a thrombin (Sigma) protease cleavage step before concentration to 40 mg/ml.
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bioRxiv - Physiology 2020Quote: ... Ptch1 was immunoprecipitated with anti myc-tag antibodies (Millipore, Cat# 05-724). Expression of Dhh ...
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bioRxiv - Biochemistry 2021Quote: ... monodisperse tag-free Ape1 was concentrated using 10 kDa MWCO Amicon (Millipore) to 15–20 mg/ml ...
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bioRxiv - Synthetic Biology 2021Quote: Recombinant hACE2 with a C-terminal deca-histidine tag (SAE0064, Sigma-Aldrich) was resuspended in PBS to a final concentration of 50 ng/μL ...
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bioRxiv - Immunology 2021Quote: ... Antibodies to Anti-Flag-tag (F1804) for immunoprecipitation were obtained from SIGMA. Antibodies to Ub (ab7780) ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with an anti-HA epitope tag PAb (Sigma, H6908) and then with a FITC-conjugated donkey anti-rabbit secondary Ab (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase (HRP)-conjugated mouse anti-FLAG tag antibody (A8592, Sigma-Aldrich) was used to detect VH binding ...
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bioRxiv - Plant Biology 2022Quote: ... Other primary antibodies we employed were: anti-HA tag antibody (Sigma, H6908); anti-c-Myc tag antibody (Abcam ...