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Citations for Millipore Sigma :
851 - 900 of 2622 citations for Recombinant Rat Pdcd1 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant human Hepatocyte Growth Factor (HGF) was obtained from Sigma Aldrich Israel ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Systems Biology 2024Quote: ... and 500 U/mL recombinant leukemia inhibitory factor (LIF; Millipore, ESG1107) at 37 °C in 5% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Cell Biology 2023Quote: Human recombinant Cdk9/cyclin K and CDK9/cyclin T (Sigma Aldrich) were incubated with the substrate at 30°C for 30 minutes in reaction buffer (25 mM Tris-acetate (pH 7.9) ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μg/mL recombinant human albumin (Sigma-Aldrich, cat. no. A9731), and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg/ml of recombinant human insulin (Sigma-Aldrich, 19278-5ML), 2 IU/ml heparin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) (L-I) ...
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bioRxiv - Cell Biology 2020Quote: ... 10% fetal calf serum or FCS (Sigma-Aldrich, St. Louis, MO, USA) as well as 1% antibiotics (penicillin and streptomycin) ...
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bioRxiv - Cancer Biology 2019Quote: ... with 10% Fetal Calf Serum (FCS) (Sigma-Aldrich, St Louis, MO, USA) and 2 mM L-Glutamine (BE17-605F ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Cancer Biology 2020Quote: ... All media were supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich) and all cells were kept at 37°C with 5% CO2 ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... containing 7 % fetal calf serum (FCS) (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 1% GlutaMAX (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... + 10% fetal calf serum (FCS; Sigma Aldrich, St. Louis, Missouri, United States) on plastic or in PRIME-XV MSC Expansion XSFM medium (FUJIFILM Irvine Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 10% FCS and supplemented when indicated with 1 μM thapsigargin (TG, Sigma).
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bioRxiv - Cancer Biology 2021Quote: ... Digested tissue was re-suspended in 10% FCS (Capricorn) DMEM (Sigma Aldrich), Pen/Strep (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were blocked in 1 % BSA and 4% FCS (Sigma-Aldrich) buffer and incubated for 30 min with primary and secondary antibodies (listed above ...
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bioRxiv - Cell Biology 2020Quote: ... Chemotaxis experiments towards 2.5% FCS and 100 ng/ml HGF (#H9661, Sigma) in DMEM were performed with μ-slide chemotaxis chambers (Ibidi ...
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bioRxiv - Biochemistry 2022Quote: ... horseradish peroxidase (HRP)-conjugated anti-human IgG Fc specific antibody (Sigma, USA) was added at the dilution of 1:2,000 and incubated at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... with goat anti-human Fc-specific IgG (Sigma-Aldrich – Saint Louis, MO) immobilized with a target capture level of ~70 RU ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% heat inactivated foetal calf serum (FCS; Sigma Aldrich, Germany), 100 U/ml penicillin and 100U/ml streptomycin ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM containing 2% FCS and 0.6% Methylcellulose (Sigma-Aldrich) was added to the cells ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10 % foetal calf serum (FCS; Sigma - catalogue no. F-7524), 1,000 units/ml LIF ...
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bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Pathology 2020Quote: ... 5-azacytidine (5-aza, Sigma-Aldrich; 1 µM in 10% FCS DMEM) for 7 days according to previous publications12 ...
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bioRxiv - Immunology 2019Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma), 2mM (1% v/v ...
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bioRxiv - Immunology 2021Quote: ... with 10% FCS and 2 mM L-Glutamine (Sigma, Cat No. G7513) and 1% penicillin/streptomycin (Invitrogen cat no ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS superior, Millipore, S0615), 2 mM L-glutamine (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10 % (v/v) FCS (Biochrom, Sigma-Aldrich or PAN-Biotech) and 1 % (v/v ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). To enrich for MSCs ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). After blocking ...
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bioRxiv - Immunology 2022Quote: ... blocking of Fc receptors with human Ig (Sigma-Aldrich, St. Louis, MO); surface staining with mouse anti-human CD3-APC-H7 ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). For analysis of neutrophil activation ...
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bioRxiv - Cell Biology 2023Quote: ... was mixed with unconjugated Fc-antibody (5 µg/ml, Sigma-Aldrich, # I2136) in HBSS and incubated for 30-60 minutes at room temperature with gentle agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Hybridoma cells were selected in DMEM/10% FCS containing HAT (Sigma, MO) and 1% BM-Condimed (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich; St. Louise, MO), penicillin streptomycin glutamine (100 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Biochemistry 2024Quote: ... the supernatant supplemented with 1% Pen/Strep and 10% FCS (Sigma-Aldrich) and stored in the dark at 4°C.
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bioRxiv - Bioengineering 2024Quote: ... or horseradish peroxidase-conjugated goat anti-human IgG Fc antibody (Sigma, A0170) diluted 1:20,000 in the blocking buffer for an hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... COS-7 (hamster, DSMZ Germany, ACC 60), and Rat-1 (rat, ATCC, CRL-2210) cells were cultured in DMEM (Sigma-Aldrich D6429) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Biochemistry 2020Quote: 3XFLAG-tagged Nth1 was immunoprecipitated using Anti-FLAG™ M2 magnetic beads (Sigma, product number: M8823). Magnetic beads were washed three times with wash buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Plant Biology 2021Quote: ... Affinity purification of tagged RPL18-containing polysomes was carried out using anti-FLAG beads (Sigma-Aldrich) incubated with the supernatant at 4 °C for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 constructs were visualised with primary antibody mouse α-FLAG (F1804; 1/200; Sigma) for 2 hours at 37°C in a humidified chamber ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...