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Citations for Millipore Sigma :
851 - 900 of 8860 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... coli strain Rosetta2 pLysS (EMD Millipore) by induction with 0.25 mM IPTG at 20°C for 16 hours.
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bioRxiv - Bioengineering 2023Quote: ... coli BL21 (DE3) cells (Sigma-Aldrich) cultured in 2x YT medium supplemented with the antibiotics of ampicillin ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 Star (DE3) cells (Novagen) as described ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21 (DE3) cells (Sigma-Aldrich) cultured in 2x YT medium supplemented with the antibiotics of ampicillin ...
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bioRxiv - Neuroscience 2023Quote: ... coli O111:B4 (Sigma-Aldrich; L2630) to induce fever and sickness ...
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bioRxiv - Microbiology 2023Quote: ... coli Rosetta 2(DE3)(pLysS) (Novagen) chemically competent cells were used for PlzAWT ...
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bioRxiv - Biophysics 2023Quote: ... coli Rosetta 2 pLysS (EMD Millipore), and 1 L cultures were grown at 37°C in 2XYT media to an OD600 of 0.6 before induction with 0.25 mM IPTG at 20°C for 16-18 hours ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) competent cells (Novagen). M9 minimal-based media were used as for TET2 ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) cells (EMD-Millipore) and were refolded and purified similarly to ALK1 ...
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bioRxiv - Immunology 2024Quote: ... coli BL21 (DE3) strain (Novagen, Merck) by overnight culturing at 30 °C in an auto-induction medium ...
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bioRxiv - Molecular Biology 2024Quote: ... coli serotype 055:B5 (Sigma, USA) samples (1 and 0.2 mg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) competent cells (Novagen). The plasmid is available at AddGene (deposition number 182428) ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells (Sigma-Aldrich) and purified as described previously [47] ...
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bioRxiv - Biochemistry 2024Quote: ... coli polar lipid extract (Sigma-Aldrich) used in PLs transport assay (70 % POPG ...
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bioRxiv - Biochemistry 2024Quote: ... coli polar lipid extract (Sigma-Aldrich). Lipids were dried under nitrogen flow and resuspended in Tris buffer by bath sonication ...
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bioRxiv - Immunology 2024Quote: ... coli 055:B5 LPS (L2880, Sigma) at 50 mg/kg ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli strain BL21 (DE3) (Novagen) was employed for protein production ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) competent cells (Novagen) were transformed and clones were selected using LB-agar medium supplemented with 25 μg/mL kanamycin and confirmed by Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Immunology 2020Quote: ... Mice received e-cigarette vapor produced from e-liquid containing 70/30 propylene glycol and glycerol with nicotine (Sigma) at a concentration of 6 mg/mL or without nicotine ...
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bioRxiv - Neuroscience 2022Quote: ... and 1μM recombinant human insulin (Sigma). Organoids were collected from the wells on day 14 and transferred to 10cm dishes at roughly 20 organoids per dish ...
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bioRxiv - Physiology 2022Quote: ... 500μg/ml human recombinant albumin (Sigma), 213μg/ml L-ascorbic acid (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... and recombinant chondriotinase ABC (Sigma, C3667) was treated at 25 mU/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant active AMPK (Millipore, 14-840) was added and incubated for 30 min at 30°C ...
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bioRxiv - Immunology 2020Quote: ... human recombinant TNF (H8916, Sigma-Aldrich) or chemical inhibitors (SB203580 from Selleck ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant IFNα was purchased from Sigma (Interferon-αA/D human Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... and Recombinant mouse LIF (Sigma, #ESG1107). Additionally ...
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bioRxiv - Immunology 2021Quote: ... Recombinant purified Angiotensin II (Sigma – A9525) at 1 μM with 2-fold serial dilutions ...
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bioRxiv - Immunology 2022Quote: ... recombinant complement component C3 (Millipore Sigma), recombinant CD4 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant leukemia inhibitory factor (SRP3316; Sigma), growth hormone (869008 ...
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bioRxiv - Neuroscience 2023Quote: ... and/or recombinant Fibronectin (Sigma-Aldrich) were applied as described using a range of concentrations (μg/ml).
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bioRxiv - Biophysics 2023Quote: ... superoxide dismutase (bovine SOD, recombinant, Sigma) was added from a ≥50,000 U/mL stock prepared in assay buffer ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant human TNFα (Sigma Aldrich, #H8916), PGE2 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: BG4 recombinant antibody (Sigma Aldrich MABE917)- ChIP
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 mIU/ml recombinant hCG (Sigma), and 10 mg/ml of human serum albumin (Sage) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000U/ml ESGro recombinant LIF (Millipore), 1uM PD0325901 (R&D Systems) ...
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bioRxiv - Developmental Biology 2024Quote: ... 10nM recombinant human gastrin (Sigma; G9145), 5ng/mL recombinant human HGF (PeproTech ...
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bioRxiv - Cell Biology 2024Quote: ... Control conditions included a set of micropattterned grids that were not incubated in antibody (No Ab) and another set of micropatterned grids that were treated with monoclonal antibodies to VZV glyco-protein E (gE; EMD Millipore Cat# MAB8612).
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bioRxiv - Systems Biology 2020Quote: ... coli K12 whole cell lysates were analysed using 10% Mini-PROTEAN Protein Gels (4568034, Bio-Rad, Laemmli buffer S3401-VL, Sigma Aldrich). One part was stained using Coomassie Blue for total protein content (Brilliant Blue G solution ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
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bioRxiv - Cell Biology 2021Quote: ... P3 2M (AMS biotechnology #GSC-6201G 2M or #GSC-6101G 7M) and LIF ESGRO® Recombinant Mouse LIF Protein (1000 units/mL) (Millipore # ESG1107). For induction of the transgene Stemolecule Doxycycline hyclate 10 mg (Stemgent#04-0016 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pelleted cells were lysed and used to purify the recombinant proteins with glutathione–Sepharose 4B beads (Sigma Aldrich #GE17-0756-01). The purified protein or whole cell lysate was incubated with the respective agarose beads at various time points with continuous agitation and washed before imaging ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μg/ml anti-E-cadherin blocking antibody that targeted the extracellular domain of E-cadherin (Gumbiner, Stevenson and Grimaldi, 1988) (uvomorulin, monoclonal anti-E-cadherin antibody, Sigma); after incubation ...
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bioRxiv - Microbiology 2020Quote: ... stained for VZV glycoprotein E (gE) (MAB8612, Millipore) in BD PermWash ...
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bioRxiv - Cell Biology 2020Quote: ... E@sy Oligo primers (Millipore-Sigma, Burlington, MA) or TaqMan Assays (Life Technology ...
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bioRxiv - Biochemistry 2019Quote: Proteinase K (E. Album) was purchased from Sigma and used without further purification ...