Labshake search
Citations for Millipore Sigma :
851 - 900 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with an Applied Biosystems 7500 Real-Time PCR system using KiCqStart®SYBR®Green qPCR ReadyMix™ (Millipore-Sigma). Gene expression was normalized to the internal control 18S rRNA ...
-
bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
-
bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed in the Applied Biosystems 7500 FAST real-time PCR system using SYBR Green JumpStart Taq ReadyMix (Sigma-Aldrich, St Louis, US). Transcript levels of target genes were determined via the 2-ΔΔCt method (Livak & Schmittgen ...
-
bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with an Applied Biosystems 7500 Real-Time PCR system using KiCqStart®SYBR®Green qPCR ReadyMix™ (Millipore-Sigma). Gene expression was normalized to the internal control 18S rRNA ...
-
bioRxiv - Microbiology 2020Quote: ... Samples for qRT-PCR were homogenized in TRiZOL (Sigma Aldrich) reagent and further processed for RNA extractions.
-
bioRxiv - Biophysics 2020Quote: ... PCR was performed with KOD Hot Start DNA polymerase (Novagen) and oligonucleotides were synthesized by Sigma Aldrich ...
-
bioRxiv - Neuroscience 2019Quote: ... PCR was completed using RedExtract ReadyAmp Taq (Sigma, SIG-R4775) with the following cycle conditions (29 cycles) ...
-
bioRxiv - Immunology 2019Quote: ... Indexed PCR product was separated on 2%-LMP agarose (Sigma) and purified with the Gel Extraction Kit (Qiagen).
-
bioRxiv - Biochemistry 2019Quote: ... Venor GeM PCR-based mycoplasma detection kit was from Sigma. ECL substrate and ECL-Plus substrate were from Pierce and were used as directed ...
-
bioRxiv - Genetics 2020Quote: ... the PCR products were separated on 2% agarose gel (Sigma) for 2 h at 70V ...
-
bioRxiv - Microbiology 2021Quote: ... PCR reactions used either KOD Hot Start DNA Polymerase (Sigma) or iProof DNA Polymerase (Bio-Rad) ...
-
bioRxiv - Neuroscience 2019Quote: ... DNA oligonucleotides (qRT-PCR primers) were obtained from Sigma-Aldrich.
-
bioRxiv - Microbiology 2021Quote: ... a PCR was performed with the KOD DNA polymerase (Novagen) to amplify the promoter and the resistance gene expression cassette with primers ML4154/ML4155 that also carry 30 bp homology with the 5 end of the TgNFS2 gene ...
-
bioRxiv - Microbiology 2021Quote: ... a PCR was performed with the KOD DNA polymerase (Novagen) to amplify the tag and the resistance gene expression cassette with primers ML5116/ML5117 (TgSDHB ...
-
bioRxiv - Microbiology 2021Quote: ... a PCR was performed with the KOD DNA polymerase (Novagen) to amplify the tag and the resistance gene expression cassette with primers ML3978/ML3979 (TgNFS2 ...
-
bioRxiv - Systems Biology 2021Quote: ... PCR products were separated in gels containing 1% agarose (Sigma) in Tris-acetate buffer (TAE) ...
-
bioRxiv - Systems Biology 2020Quote: ... Linear products were created by PCR with primers from Sigma Life Science and Q5 Polymerase (New England Biolabs ...
-
bioRxiv - Biochemistry 2022Quote: ... The PCR product was incorporated into the pET28a(+) vector (Novagen) between its NdeI and Xhol restriction sites using standard molecular biology protocols ...
-
bioRxiv - Biochemistry 2022Quote: ... The PCR product was cloned into pET-19b plasmid (Novagen) and sequence verified ...
-
bioRxiv - Cancer Biology 2022Quote: ... Quantitative RT-PCR was performed using Kapa SYBR Fast (Sigma). SREBP genes primer sequences are listed in Supplementary Table S3 ...
-
bioRxiv - Microbiology 2022Quote: ... a PCR was performed with the KOD DNA polymerase (Novagen) to amplify the promoter and the resistance gene expression cassette with primers ML4107/ML4108 that also carry 30□bp homology with the 5□ end of the TgSUFC gene ...
-
bioRxiv - Bioengineering 2019Quote: ... premix REDTaq® ReadyMixTM PCR Reaction Mix (Sigma-Aldrich, USA,) was used ...
-
bioRxiv - Cell Biology 2019Quote: ... The PCR products were revealed by ethidium bromide (Sigma-Aldrich) staining ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Taq DNA polymerase and PCR buffers were purchased from Sigma, India ...
-
bioRxiv - Genomics 2020Quote: ... qRT-PCR was performed with KAPA SYBR® FAST (Sigma) on a Rotor-Gene Q (Qiagen).
-
bioRxiv - Molecular Biology 2020Quote: RT-PCR: 106 cells were lysed in TRI reagent (Sigma) and RNA extracted using Direct-zol columns with a 30 minute on-column DNase step at room temperature (Zymo Research) ...
-
bioRxiv - Molecular Biology 2019Quote: ... qRT–PCR was performed with KAPA SYBR® FAST (Sigma) on a Rotor-Gene Q (Qiagen) ...
-
bioRxiv - Biochemistry 2020Quote: ... PCR stages used the KOD HotStart Polymerase Kit (Sigma-Aldrich); In-gel DNA extraction steps used the E.Z.N.A gel extraction kit (omega BIO-TEK) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... PCR products were purified using the GenElute kit (Sigma Aldrich) and concentrations were checked using a NanoDrop ND1000 (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... Cells were mycoplasma free (LookOut Mycoplasma PCR detection kit, Sigma). Cells were passaged with 0.05% trypsin-EDTA every 2-3 days ...
-
bioRxiv - Cell Biology 2019Quote: ... Linear products were created through PCR with primers from Sigma Life Science and Q5 Polymerase (New England Biolabs ...
-
bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
-
bioRxiv - Genetics 2020Quote: ... each PCR well contained: 10 μL REDTaq ReadyMix (Sigma-Aldrich), 8.2 μL dH2O ...
-
bioRxiv - Microbiology 2021Quote: ... from the Kap2G Robust PCR kit (Sigma-Aldrich, Gillingham, UK) and 9 µL added to each well in a 96-well plate ...
-
bioRxiv - Microbiology 2020Quote: ... These PCR products were cloned into pET-28b(+) (Novagen, USA) to construct tagged Fnr proteins with His-tag at the N-terminus of Fnr1 and C-terminus of Fnr3 respectively ...
-
bioRxiv - Immunology 2021Quote: ... as confirmed by the LookOut Mycoplasma PCR Detection Kit (Sigma). Cell lines were authenticated by the ATCC ...
-
bioRxiv - Plant Biology 2021Quote: ... with PCR reactions using FastStart Taq DNA Polymerase (Sigma-Aldrich), which does not have proofreading activity ...
-
bioRxiv - Developmental Biology 2020Quote: ... Libraries were then amplified by PCR with TruSeq primers (Sigma) and Phusion polymerase (New England Biolabs) ...
-
bioRxiv - Genomics 2020Quote: ... Primers used for genotyping PCR were synthesized by Sigma-Aldrich. Oligonucleotide sequences are given in Table 2.
-
bioRxiv - Cell Biology 2022Quote: ... in PCR tubes containing lysis buffer (0.2% Triton (Sigma Aldrich), 0.4 U/µL RNaseOUT (Thermofisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... a PCR was performed with the KOD DNA polymerase (Novagen) to amplify the tag and the resistance gene expression cassette with primers ML3980/ML3981 ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products were separated using 1% agarose gels (Sigma-Aldrich) dyed with SYBR Safe (Invitrogen ...
-
bioRxiv - Systems Biology 2022Quote: ... the inserts were PCR amplified with KOD HotStart Polymerase (Novagen) and verified by Sanger sequencing.
-
bioRxiv - Plant Biology 2022Quote: ... The PCR products were cloned into pET15b expression vector (Novagen) between NdeI and BamHI restriction sites ...
-
bioRxiv - Neuroscience 2023Quote: ... and cDNA purification (Genelute PCR clean-up kit, Sigma-Aldrich) were carried out ...
-
bioRxiv - Plant Biology 2024Quote: ... Diagnostic PCR was conducted using JumpStart REDTaq ReadyMix (Sigma Aldrich) with one of the following primer pairs for the T-DNA ...
-
bioRxiv - Microbiology 2023Quote: ... purified with the GenElute PCR Clean-up kit (Sigma Aldrich) again ...
-
bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed with KAPA SYBR® FAST (Sigma) on Rotor-Gene RG-3000 A (Corbett Research).
-
bioRxiv - Microbiology 2023Quote: ... DNA sequencing of PCR products was performed by Sigma-Aldrich. PCR reactions were carried out with Taq DNA polymerase (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... and purified using the GenElute PCR cleanup kit (Sigma Aldrich). The DNA library was prepared using the Illumina DNA M preparation kit and sequenced on the Illumina NovaSeq 6000 platform at the AGRF ...