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Citations for Millipore Sigma :
851 - 900 of 2420 citations for NPT3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Blocking was performed at RT with 2% bovine serum album (BSA) (Sigma-Aldrich, #A7906), 2% goat serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... slices were first blocked using blocking solution (5% normal goat serum (Sigma, #G9023, Lot:SLCH5798) in 0.3% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were submerged in primary antibodies diluted in blocking buffer with 0.05% Tween20 (Sigma) and incubated at 4⁰C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... a blocking solution containing 10% goat serum (Lampire #7332500) and 0.2% TX-100 (Sigma) in PBS was applied to the tissue for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... After blocking the membrane was incubated with Monoclonal anti-FLAG HRP antibody (Sigma, F3165) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated for 1.5 h in blocking solution: 5% Donkey Serum (Sigma, S30); 0.3% Triton X-100 ...
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bioRxiv - Immunology 2022Quote: ... After blocking with 5 % skimmed milk in TBS-T (0.1 % Tween-20 (Sigma-Aldrich) in 1x Tris-buffered saline ...
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bioRxiv - Pathology 2023Quote: ... after blocking nonspecific binding sites with 10% bovine serum albumin (A7906, Sigma Aldrich, USA), 0.5% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... nuclei were stained in blocking buffer containing 10 µg/ml Hoechst 33342 (Sigma-Aldrich) for 10 min at 28°C ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with blocking solution (3% BSA fatty-acid free (Sigma-Aldrich) in PBS (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Rehydrated membranes were blocked in 5% blocking buffer containing skim milk (Sigma, Cat.No. 1153630500) in 0.1% PBST for 1½ hrs and then incubated with primary antibodies diluted in 5% blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and briefly washed with blocking solution containing 4% normal donkey serum (NDS, Sigma- Aldrich) and 0.25% Triton-X (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were first incubated in blocking solution (2% bovine serum albumin [Sigma-Aldrich] and 0.3% triton X-100 [ThermoFisher] in 1X PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Then washing with ddH2O again and followed by blocking 3% BSA (SIGMA, V900933-100G) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody was applied overnight at 4°C in blocking buffer (α-Actinin, Sigma A7811 ...
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bioRxiv - Immunology 2023Quote: ... diluted in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with function-blocking anti-ß1-integrin monoclonal antibodies (MAB1987Z, Millipore Sigma) at a concentration of 10 µg/ml and fixed 24 hours later ...
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bioRxiv - Cell Biology 2023Quote: ... followed by blocking and permeabilization step in 1% bovine serum albumin (Sigma, A9647-50G) in PBST (0.2% Triton X-100 in PBS ...
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bioRxiv - Biophysics 2023Quote: ... After 1-hour incubation in blocking buffer (3% bovine serum albumin (BSA, Sigma-Alrich) in BRB80 buffer) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were saturated in a blocking solution (3% bovine serum albumin (BSA) (Sigma-Aldrich), 5% donkey serum (Merck Millipore) ...
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bioRxiv - Immunology 2023Quote: ... Blocking buffer and antibodies were diluted in 5% non-fat dry milk (Sigma-Aldrich) in TBS-Tween 0.1% ...
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bioRxiv - Molecular Biology 2023Quote: ... After pre-incubation with blocking buffer [PBS supplemented with 1% BSA (Merck Millipore/Sigma) and 0.1% Triton X-100] at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... After pre-incubation with blocking buffer [PBS supplemented with 1% BSA (Merck Millipore/Sigma) and 0.1% Triton X-100] at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Permeabilisation and blocking were performed simultaneously by incubating samples with 1% BSA (Sigma Aldrich) in PBS with 0.5% Triton X-100 for 3 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS (PBST) at RT followed by blocking solution (PBST + 4% BSA (Sigma A9647)) ...
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bioRxiv - Immunology 2024Quote: ... Blocking was performed in PBSTA (PBS supplemented with 1% w/v BSA (Sigma Aldrich) and 0.05% v/v Tween-20 ...
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bioRxiv - Immunology 2024Quote: ... 150 µL of blocking buffer (2% bovine serum albumin (Sigma, USA #9048-46-8) in washing buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with 100 μg/ml of 3×FLAG peptide (Sigma). The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 125 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS ...
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bioRxiv - Cell Biology 2022Quote: ... Elution was achieved by using 250 μg/ml 3 × Flag peptides (F4799, Sigma). The resulting eluates were resuspended in TRIzol for the following RNA extraction ...
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bioRxiv - Cell Biology 2019Quote: ... and rat/mouse c-peptide ELISA kit (cat # EZRMCP 2-21K, Millipore MA).
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bioRxiv - Microbiology 2020Quote: ... peptides eluted from gel slices were purified on ZipTip C18 columns (Millipore, Germany) and applied to a stainless steel target together with α-cyano-4-hydroxycinnamic acid as a matrix ...
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bioRxiv - Cancer Biology 2019Quote: ... Extracted peptides samples were purified and concentrated using ZipTip pipette tips (Millipore Corporation) following the manufacturer’s directions ...
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bioRxiv - Immunology 2020Quote: ... The labelled peptide pools were desalted using P10 C18 pipette ZipTips (Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Peptide samples are further cleaned up and concentrated using Zip Tips (EMD Millipore). Zip Tips are 10 μL pipette tips with a 0.6 μL bed embedded with a very small ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were eluted with 150 μg.ml-1 3× FLAG peptide (Sigma-Aldrich, F4799) in extraction buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptides were desalted with C18 ZipTip pipette tips (Merck Millipore, ZTC18S960) and loaded onto an Ultimate 3000 UPLC system (Dionex ...
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bioRxiv - Physiology 2021Quote: ... Elution of the immunoprecipitates was performed with 3× FLAG peptide (F4799, Sigma-Aldrich). The immunoprecipitation procedure was validated by western blotting using FLAG and Fam64a antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Bound polypeptides were eluted stepwise with 250 μg/ml FLAG peptide (Sigma Aldrich) diluted in BC100 buffer ...
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bioRxiv - Microbiology 2021Quote: ... The cleaved peptides were treated with 1/20 volume of formic acid (Sigma), and the samples were stored at - 20 °C until LC/LC–MS/MS analysis.
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bioRxiv - Biophysics 2020Quote: ... A final concentration of 0.2 mg mL-1 3X FLAG peptide (Sigma, F4799) in the RNC buffer was used to release the RNCs from the beads ...
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bioRxiv - Cell Biology 2022Quote: ... The bound material was eluted with 200 μg/mL FLAG peptide (Sigma, F3290). The purified samples were then passed through a PD-10 column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... the immunoprecipitation was outcompeted with the 3XFLAG peptide (340 µM; Sigma-Aldrich, F4799). Beads were then washed 6 times with buffer A with 0.1% Triton X-100 followed by elution with Laemmli buffer ...
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bioRxiv - Neuroscience 2021Quote: ... To elute proteins from the beads a 3xFLAG peptide (100 ug/mL, Sigma) with 0.05% RapiGest in IP buffer was added to the beads for 15 minutes at room temperature with agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were competitively eluted using 3X FLAG peptide (Sigma; in Myc wash buffer). The eluted FLAG-ACBD5 was incubated with the Myc-VAPB-bound beads for 1 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Bound polypeptides were eluted stepwise with 250 μg/ml FLAG peptide (Sigma Aldrich) diluted in BC100 buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and proteasome was eluted by adding 3x FLAG peptide (400 μg/ml) (Sigma) in Buffer A”.
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bioRxiv - Systems Biology 2022Quote: ... beads were immersed in elution buffer (0.15 ug/mL 3X Flag peptide (Sigma)) and agitated on rotisserie for 1 hour prior to elution ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide stock solutions were diluted with washing buffer (0.1% NP40 substitute (Sigma 74385) in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... The fractions containing the peptides were pooled and concentrated with an Amicon (Millipore) centrifugal concentrator ...