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Citations for Millipore Sigma :
851 - 900 of 3776 citations for Human MXRA8 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... blotted onto nitrocellulose and the proteins were detected with an anti-His antibody (Sigma-Aldrich, Steinheim, Germany). To test whether 6His-PBBem1 and Cdc11 can bind simultaneously to Cdc24428-854 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were then added directly to 96-well His-Select Ni-NTA resin filter plates (Sigma Aldrich) and processed off-robot by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Biochemistry 2023Quote: ... After several rounds of washing with His-AC washing buffer (supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Human ovarian cancer A2780 and Adriamycin resistant human ovarian cancer A2780-R cells were procured from Sigma Inc ...
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bioRxiv - Genetics 2022Quote: ... Human Erythroleukemia (HEL) and human TF-1 cells were cultured in RPMI-1640 medium (Sigma Aldrich #R8758) supplemented with 10% Fetal Bovine Serum (Sigma Aldrich #F0926 ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte- human fibrinogen mixture (Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte-human fibrinogen mixture (Sigma-Aldrich) and 2µL of the mixture is carefully pipetted into the microwell ...
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bioRxiv - Neuroscience 2021Quote: ... which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629, Sigma-Aldrich). This plasmid was verified by sequencing and used throughout the study ...
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... 8 µg antibody (Monoclonal ANTI-FLAG® M2, Art. No. F3165, Sigma-Aldrich® / Myc-Tag (9B11) Mouse mAb ...
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bioRxiv - Biochemistry 2022Quote: ... the GST-tag of GFP-N-WASP-VCA and cortactin was cut by PreScission Protease (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies against FLAG tag [M2] (F1804, RRID: AB_262044) and [M2] (F3145, RRID:AB_259529) were purchased from Sigma Aldrich. Antibodies against DDX3X [D19B4] (8192S ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in phosphate-buffered saline (PBS) containing 1/400 anti Rho-tag antibody 4D2 (Millipore), 15mM NaN3 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the eluate was analysed by SDS-PAGE and western blotting with anti-His6-tag antibody (Sigma).
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... the mFc and 8xHis tags at the C-terminus were removed by HRV-3C protease (Millipore-Sigma) and then purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the mFc and 8xHis tags at the C-terminus were removed by HRV-3C protease (Millipore-Sigma) and then purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... respectively) was cloned into a pRSF-Duet vector preceded by an N-terminal His6-SUMO tag (Novagen)[11 ...
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bioRxiv - Immunology 2020Quote: ... rabbit anti-SARS-CoV S polyclonal sera (BEI Resources) and mouse anti-2xStrep-tag antibody (Sigma-Aldrich). Anti-mouse IgG AF555 ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoblots from tandem affinity purifications were probed with antibodies against S-tag (mouse monoclonal MAC112; EMD Millipore) followed by visualization using IRDye-tagged secondary antibodies.
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bioRxiv - Molecular Biology 2021Quote: ... The upper portion of the membrane was probed with anti-HA tag antibody (mouse, Sigma-Aldrich, H3663) diluted 1:1,000 in 5% skim milk solution ...
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bioRxiv - Biochemistry 2020Quote: ... Phage with uncleaved FLAG-tag were removed by immunodepletion using M2 anti-FLAG monoclonal antibody (Sigma-Aldrich) coupled to epoxy-activated M-450 magnetic beads (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: TAG and cholesterol evaluation of tissue and serum were processed according to the manufactures’ instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). 5hmdCTP/dGTP/dATP/dTTP mix was purchased from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). E ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized human haptoglobin phenotype 1-1 (Hp) and human α,β haemoglobin (Hb) were purchased from Sigma Aldrich. Deglycosylation enzyme PNgase F was from New England BioLabs Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Plant Biology 2019Quote: ... The transformants were then spotted on SD (-Trp -Leu -His) selection media containing 0.5/1mM 3-Amino-1,2,4-triazole (3-AT; Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...