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Citations for Millipore Sigma :
851 - 900 of 1006 citations for FITC SA ZAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Mice were fasted for 4hrs before oral gavage with 200uL of fluorescein isothiocyanate (FITC)-dextran (3-5 kDa, Sigma-Aldrich) dissolved in sterile PBS (60mg/ml) ...
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bioRxiv - Immunology 2019Quote: ... Fluorescein isothiocyanate (FITC) [2-(6-hydroxy-3-oxo-3h-xanthen-9-yl)-5-isothiocyanatobenzoic acid] was purchased from Sigma-Aldrich Korea ...
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bioRxiv - Developmental Biology 2019Quote: ... WT and Etv5 KO mESCs (~2×105 cells) were collected and resuspended with 1×Binding Buffer containing Annexin V-FITC/PI (Sigma). The cells were incubated at room temperature in the dark for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated for two nights at 4°C with mouse ACTA2-FITC antibodies (1:200; Sigma, F3777) in blocking buffer containing 1-5% bovine serum albumin in 0.25 – 0.5% Triton-X 100 in 1× PBS ...
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bioRxiv - Neuroscience 2020Quote: ... was injected into the heart chamber of the fish along with a high molecular weight fluorescent dye (2000 kDa dextran-conjugated FITC (3 mg/ml, Sigma). We estimate that 1 nl of a 10 nM Aβ injection into a ∼3.01 (±0.16 ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized and the carotid artery was cannulated for the application of fluorescein isothiocyanate (FITC)–dextran (Sigma-Aldrich, 150kD) as described elsewhere (57) ...
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bioRxiv - Cell Biology 2020Quote: ... cell-surface mannoproteins were stained by 1 mg/mL fluorescein isothiocyanate (FITC)-conjugated concanavalin A (Sigma-Aldrich, St. Louis, MO) in P buffer (10 mM sodium phosphate and 150 mM NaCl [pH 7.2] ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with respective FITC- or Alexa Fluor 647-conjugated secondary antibody (Sigma-Aldrich F9137 or Thermo Scientific A21244) at a dilution of 1:100 for 1 h at room temperature ...
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bioRxiv - Physiology 2021Quote: ... Frozen sections were used to visualize cardiomyocyte cell membranes by staining with FITC-conjugated wheat-germ agglutinin (Sigma-Aldrich, USA), as described before Zhao et al ...
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bioRxiv - Microbiology 2021Quote: Protease assays were performed with a protease fluorescent detection kit using casein labeled with fluorescein isothiocyanate (FITC) as the substrate following the manufacturer’s instructions (PF0100, Sigma-Aldrich). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... a fluorescein (FITC) labelled 4.4 kDa dextran fraction (negative overall charge conferred to dextran by fluorophore charge) (Sigma-Alrich, FD4) or a FITC labelled 4.4 kDa dextran fraction modified with diethylaminoethyl (DEAE ...
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bioRxiv - Bioengineering 2022Quote: ... CUBE filled with 1.5% agarose was placed in the gradient chip with 10 μM 40kDa fluorescein isothiocyanate (FITC)-dextran (Sigma, FD40S) in DPBS at one end of the CUBE ...
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bioRxiv - Microbiology 2023Quote: ... Parasites attached to coverslips were fixed in 4% paraformaldehyde at room temperature for 20 min and labeled with wheat germ agglutinin (WGA) lectin from Triticum vulgaris conjugated with FITC (Sigma). To this end ...
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bioRxiv - Neuroscience 2022Quote: ... mice were intravenously (via the tail vein) administered 2% w/v FITC-dextran in saline (70,000 Da; Sigma-Aldrich, USA) 10 minutes prior to imaging ...
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bioRxiv - Microbiology 2022Quote: Mice were fasted overnight and gavaged the next morning with 80 mg of 3000-5000 kDa FITC-dextran (Sigma-Aldrich) per 100 g bodyweight ...
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bioRxiv - Molecular Biology 2022Quote: ... The parasites were supplemented with 10µM DNA-cholesterol nanoparticles and 5mg/mL fluorescein isothiocyanate (FITC)-dextran with a mean molecular mass of 10kDa (Sigma-Aldrich) as a fluid phase marker ...
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bioRxiv - Cell Biology 2022Quote: ... Dylight 550-labeled rabbit anti-BUB-1 (custom made, 74) and FITC-labeled mouse anti-α-tubulin (SIGMA F2168, DM1α) were used at a concentration of 1 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... protein (1:50 dilution) (BEI Resources, NR-619) and the secondary anti-mouse IgG antibody conjugated with FITC (1:100 dilution, Sigma). The cell nuclei were stained with DAPI ...
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bioRxiv - Genetics 2023Quote: ... the isolated cells were characterized by flow cytometry using the SMC-specific FITC-labeled anti-α-SMA antibody (Sigma, F3777) and an isotype control (mouse IgG2a-FITC ...
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bioRxiv - Cell Biology 2023Quote: ... mice were fasted overnight and then orally gavaged with 150 μL of 10 mg/mL FITC-dextran (Sigma-Aldrich, FD4) in DPBS (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently incubated with 5 μL of annexin V-FITC and 10 μL of propidium iodide (Sigma-Aldrich, USA) staining solution for 15 min at room temperature in the dark ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant solution was removed and the cells were treated with a secondary antibody (the FITC-conjugated anti-mouse IgG (Sigma)) for one hour at 37°C in the darkness ...
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bioRxiv - Immunology 2023Quote: ... mice were fasted for four hours before oral gavage of 600 mg/kg FITC–3-5-kDa dextran (Sigma, FD4). Serum was collected six hours after gavage as well as control mice without gavage and analyzed using a Synergy HT plate reader (Bio-Tek ...
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bioRxiv - Bioengineering 2022Quote: ... 500 μl of 4 kDa (100 μg/ml) or 70 kDa (500 μg/ml) fluorescein isothiocyanate (FITC)-dextran (Sigma-Aldrich) was added to the insert and 1 ml of cell culture medium was added to the low compartment ...
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bioRxiv - Immunology 2023Quote: ... followed by streptavidin-Cyanine-3 (1:500; Amersham Bioscience) or by goat anti-human IgG coupled to FITC (1:100, Sigma), as appropriate ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were inoculated at MOI 15 in 1 mg/mL of fluorescein isothiocyanate–dextran 4 kDa (FITC-dextran, Sigma-Aldrich) dissolved in CnT-Prime-3D ...
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bioRxiv - Neuroscience 2023Quote: ... mice were then perfused with prewarmed (37°C) FITC-albumin gel [2.5% w/v gelatin (Sigma-Aldrich, cat. #G1890-1KG), 0.05% w/v FITC-albumin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... PdgfrβCre-tdTomato mice were injected with 25 µL of 5% (w/v in saline) 70kDa FITC-dextran (Sigma-Aldrich; 46945), PdgfrβCre-tdTomato ...
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bioRxiv - Cancer Biology 2023Quote: ... the unencapsulated FITC-dextran or FITC-SAP was removed by washing and filtration using Amicon Ultra centrifugal filters (100 K device, Merck Millipore). The incorporation of FITC-dextran and FITC-SAP into the nsEVs was confirmed by spectrofluometer analysis (FP-6200 ...
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bioRxiv - Cancer Biology 2023Quote: ... apoptosis was measured by incubating 1 million cells for 1 hour in 1 microliter/ml FITC-VAD-FMK (EMD-Millipore) to detect apoptotic cells by flow cytometry ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured in 0.4 μm or 3.0 μm pored Transwell inserts and fluorescein isothiocyanate (FITC)-conjugated dextran molecules of 3–5 kDa or 150 kDa (Sigma) were added at 0.5 mg/ml to the top chamber of the Transwell inserts as we previously described41 ...
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bioRxiv - Neuroscience 2023Quote: Gut permeability was measured using oral administration of fluorescein-isothiocyanate-labeled dextran (FITC-dextran; molecular weight 4kDa; Sigma- Aldrich 46944). Adult male and female rats (P60 ...
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bioRxiv - Bioengineering 2023Quote: Medium in the apical perfusion channel was replaced by medium containing 1 mg/ml FITC-dextran (40 kDa, Sigma, FD40). To evaluate barrier integrity imaging was performed at different time-points ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Developmental Biology 2023Quote: ... at E21.5 rat fetuses were placed under isoflurane anesthesia and intracardiac perfusion was performed with PBS to wash out blood components followed by 1mg/ml 10,000 Da FITC (Sigma Aldrich) in PBS (pH 7.0 ...
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bioRxiv - Neuroscience 2024Quote: Small intestine transit time: A solution containing fluorescein isothiocyanate-dextran (FITC-Dextran, 100 μl; 10 mg/ml in 2% methylcellulose; FD70, Sigma) was administered to mice by gavage through a 21-gauge round-tip feeding needle ...
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bioRxiv - Neuroscience 2024Quote: ... cells were cultured in 0.4 µm or 3.0 µm pored Transwell inserts and fluorescein isothiocyanate (FITC)-conjugated dextran molecules of 3– 5 kDa or 150 kDa (Sigma) were added at 0.5 mg/ml to the top chamber of the Transwell insert for 24 h ...
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bioRxiv - Biophysics 2024Quote: ... The slides were then rinsed with PBS (3×) and then stained with an in situ cell death detection (FITC-TUNEL) staining kit (Millipore), an AlexaFluor-647 anti-collagen antibody (NovusBio ...
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bioRxiv - Microbiology 2020Quote: ... cholera toxin B subunit (CtxB)-FITC conjugate (C1655), methyl-β-cyclodextrin (Mβ-CD, C4555) and chlorpromazine hydrochloride (CPZ, C8138) were procured from Sigma.
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bioRxiv - Cell Biology 2020Quote: ... and permeabilized with 0.1 % triton X-100 for 10 mins for intracellular staining with a mouse or rabbit monoclonal antibody against the target protein (1h) followed by a secondary antibody conjugated with FITC- or Alexa Fluor 647 (Sigma-Aldrich) (1.100 ...
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bioRxiv - Pathology 2021Quote: Hydrogels (n=6/time point) were immersed in a solution of fluorescein-conjugated human IgG (IgG-FITC, final concentration 1 mg/mL) (Sigma-Aldrich) in TBS containing 0.1% (v/v ...
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with PBS for 1 h at 37°C and then incubated with a FITC-conjugated rabbit anti-mouse IgG antibody (Sigma-Aldrich) at a 1:500 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... This was followed by a rinse with phosphate buffered saline and application of the diluted primary antibodies: whole molecule Anti-Rabbit IgG (1:200, goat, FITC antibody, Sigma-Aldrich) then secondary antibodies (Whole molecule ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were cleaned in phosphatebuffered saline (PBS) for three times and stained in PI/FITC-Annexin V with 50 μg/ml RNase A (Sigma-Aldrich). Keep cells in dark processing at the room temperature for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... a blocking step of incubation with sheep serum for 1 hour was followed by immunostaining for N using a mouse monoclonal antibody (αN009 48) and detected using a sheep anti-mouse FITC conjugate (Sigma, UK). Phalloidin Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... RBCs were suspended at a concentration of 104 cells/ml in the AS-3 media supplemented with 1 mg/ml of FITC-Dextran (Sigma-Aldrich). The solution containing the cells was introduced into one of the microfluidic devices described previously and imaged using a Leica DMI 6000B inverted phase contrast microscope (Leica Camera ...
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bioRxiv - Bioengineering 2021Quote: Protein adsorption to the films was analyzed by incubation in a bovine serum albumin-FITC protein solution (1mg/ml, Sigma Aldrich) overnight at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Biochemistry 2020Quote: ... The cells were washed in DPBS (5 minutes each wash) and incubated with 2.5 µg/ml an FITC-labeled anti-HA antibody (Sigma, Product # H7411) diluted with 5% BSA in DPBS for 1.5 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: Unlabeled poly-L-lysine (molecular weight 15-30 kDa) and fluorescently labelled FITC-poly-L-lysine (molecular weight 15-30 kDa) was purchased from Sigma-Aldrich. Individual nucleotides (ATP and GTP ...