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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... and a mouse monoclonal anti-FLAG epitope-tag antibody (Sigma #F7425). For the detection of endogenous Pdlim5 ...
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bioRxiv - Microbiology 2023Quote: ... rabbit polyclonal anti-FLAG-tag (#F7425, Sigma-Aldrich, dilution 1:1,000), rabbit polyclonal anti-EGFP (#2555 ...
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bioRxiv - Neuroscience 2023Quote: The StrepII–GFP tag was cleaved by HRV 3C protease (Novagen) added at 10 U/mg of BmGr9 overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Flag tag (F3165) and C14ORF43 (HPA003111 for WB) from Sigma-Aldrich. Monoclonal antibodies against TFII-D subunits were generous gifts from Dr ...
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bioRxiv - Molecular Biology 2024Quote: ... and validated by Western Blotting for 3xFLAG tag (Antibody M2, Sigma).
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bioRxiv - Neuroscience 2021Quote: ... was used to deliver HBSS before and after application of E2 (100 pM diluted from DMSO-stocks in HBSS, 17β-estradiol, Sigma-Aldrich, cat # E-8875) or ATP (30 uM diluted in HBSS ...
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bioRxiv - Microbiology 2022Quote: ... hormones were maintained in the culture medium throughout the course of each assay. 17-ß-estradiol (i. e., E2) and progesterone (i. e., P4) (both from Sigma; St. Louis, MO) were selected for use in infection studies because these hormones represent the major forms of estrogens and of progestogens (respectively ...
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bioRxiv - Physiology 2019Quote: ... 6 dpf larvae in 35 mm Petri dishes were first incubated in 5 mL of steady state E2 medium + 100 µL of Tricaine (Sigma-Aldrich #E10521, Schnelldorf, Germany); they were considered to be anesthetized when they failed to respond to tactile stimulation ...
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bioRxiv - Immunology 2022Quote: ... For surface staining cells were incubated with mastermix prepared in Fc-block (1%FCS/PBS with 0.1% rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) ...
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bioRxiv - Developmental Biology 2019Quote: ... supplemented with 10% FCS and 1% penicillin and streptomycin (Sigma). After separation from UGE ...
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bioRxiv - Genetics 2021Quote: ... supplemented with 1 g/ml glucose containing 10% FCS (Sigma), 1% penicillin (10,000 units/ml ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 10% inactivated Fetal Calf Serum (FCS) (Sigma-Aldrich), 100 ng ml−1 L-Glutamine ...
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bioRxiv - Biochemistry 2020Quote: ... the MR-Fc was purified over mannan-agarose beads (Sigma) and protein A-agarose beads ...
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bioRxiv - Genetics 2022Quote: ... containing 10% of fetal calf serum (FCS) (Sigma-Aldrich, F7524), 1% of penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 2% FCS and 0.5µg/ml DAPI (Sigma Aldrich, Ref: D9542) and passed through a 40µm Falcon cell strainer ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% FCS (Sigma-Aldrich, #F9665-500ML, Lot #19A124) and 1% Penicillin-Streptomycin-L-Glutamine (MED30-009-CI) ...
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bioRxiv - Microbiology 2021Quote: ... 4% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma), 25 mM HEPES buffer (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... 20% FCS and 1% L-glutamine (Sigma-Aldrich, Buchs, Switzerland), as described in (41) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5% v/v heat inactivated FCS (Sigma Aldrich cat. # 12133C) and Penicillin-Streptomycin (100 units/mL) ...
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bioRxiv - Microbiology 2020Quote: ... 4% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma) and 25mM HEPES buffer (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... containing 10 % fetal calf serum (FCS) (Sigma-Aldrich, Hamburg, Germany) and 1 % penicillin / streptomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... complemented with 10 % fetal calf serum (FCS; Sigma-Aldrich F7524) and 1 % of penicillin and streptomycin mixture (Sigma-Aldrich P0781 ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10 % fetal calf serum (FCS, Sigma-Aldrich F7524) and 1 % penicillin streptomycin mixture (Sigma-Aldrich P0781) ...
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bioRxiv - Immunology 2021Quote: ... and fetal calf serum (FCS; 20% v/v; Sigma-Aldrich). Complete medium (R+ ...
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bioRxiv - Immunology 2021Quote: ... counted and cryopreserved in 90% FCS / 10% DMSO (both Sigma). PBMC aliquots were frozen in a methanol bath (to freeze slowly ~ 1C/min ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 8% (v/v) Fetal Calf Serum (FCS; Sigma), penicillin (100 U mL-1) ...
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bioRxiv - Neuroscience 2023Quote: ... These specimens were immersed in Fluorinert (FC-40, Sigma-Aldrich) and allowed to rest for four hours at room temperature before scanning ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% FCS (Biowest) and 1% Pen/Strep (Sigma).
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma F-7524), 1000 U/mL LIF ...
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bioRxiv - Immunology 2023Quote: ... and 8% fetal calf serum (FCS, Sigma-Aldrich Chemie BV). TK-1 murine T-cell lymphoma cells (CRL-2396 ...
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bioRxiv - Immunology 2023Quote: ... complete media supplemented with 10% (vol/vol) FCS (F0804, Sigma), 1% penicillin-streptomycin (15140122 ...
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bioRxiv - Immunology 2023Quote: ... 70% fetal calf serum (FCS) (Sigma-Aldric, St. Louis, MO) and 20% RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... U251 glioblastoma cells were purchased from Millipore Sigma and maintained in DMEM supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma, F7524-500ML) and 1% Penicillin-Streptomycin (Pen/Strep ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was additionally supplemented with 2.5% FCS (Sigma Aldrich) from DIV 9 onwards ...
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bioRxiv - Biophysics 2023Quote: ... which consisted of fluorinated oil (Fluorinert FC-40, Sigma-Aldrich) and 2% w/w fluorosurfactant (RAN biotechnologies ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich, F7524) and maintained in a humidified incubator at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2024Quote: ... and fetal calf serum (10%, FCS, Sigma-Aldrich-Chemie-BV)) with Geneticin G418 Sulphate (0.6 mg/ml ...
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bioRxiv - Microbiology 2022Quote: For immunofluorescence experiments the panel of HEK293 cells grown on poly-Lysine (Sigma-Aldrich Cat. Nr. P6282) pre-coated (2.5 μg/mL in MQ H2O 30 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-GlutaMAX™ -I (Sigma-Aldrich) supplemented with 10% fetal bovine serum (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (2 x 104 cells per well) were transiently transfected using X-tremeGENE™ 9 (Sigma) with plasmids encoding eYFP tagged PAR2 (PAR2-eYFP ...
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bioRxiv - Biophysics 2021Quote: ... and HEK293 control cells were separated by SDS-PAGE using TruPAGE precast gels (4-12%) (Sigma-Aldrich) or 8% Laemmli SDS-gels ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
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bioRxiv - Genetics 2021Quote: ... were superovulated by serial Pregnant Mare Serum Gonadotropin (5 IU per mouse, Prospec Protein Specialists) and human chorionic gonadotropin (5 IU, Millipore) injections 46 h apart ...
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bioRxiv - Neuroscience 2020Quote: ... SI and protein extracts from human and mouse samples were resolved in SDS-PAGE transferred to Immobilon-P PVDF membranes (Millipore). Membranes were activated with methanol and blocked with 4 % bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...