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Citations for Millipore Sigma :
851 - 900 of 1155 citations for 7 Nitrogramine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... at pH 7 and re-suspended in HBSS with 250μM HEPES and 1% bovine serum albumin (BSA) (Sigma-Aldrich, USA). The cells were sorted based on GFP intensity using “Moflo Astrios EQ” (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... An o-Phenanthroline solution was prepared mixing 30 μL of hydrochloric acid (37%) with 7 mL MilliQ water and dissolving 0.2 grams 1,10-Phenanthroline monohydrate (Sigma-Aldrich, 99%) in it ...
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bioRxiv - Immunology 2020Quote: ... 7-to 8-week old WT mice were injected intraperitoneally with 20 mg/kg body wight of LPS (Sigma, L2630). The mice were administered intraperitoneally 200 μl of DPBS containing no antibody (n = 17) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cathepsin B was measured with the fluorogenic substrate Na-CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin hydrochloride (Sigma) after a 30-min pretreatment with DTT and EDTA ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose was removed by 7 sequential washes in a centrifugal filtration unit with a molecular weight cut-off (MWCO) of 100 kDa (Millipore). Polysomes were converted into monosomes by RNase H digestion ...
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bioRxiv - Pathology 2021Quote: ... the 5th injection of ketamine or SAL included a co-injection of L-DOPA (7 mg/kg, i.p., Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... and the lysate/antibody/bead mix was incubated at 4°C overnight with continuous rotation Beads were then washed in HEPES buffer and boiled in Laemmli loading buffer prior to SDS PAGE and western blot analysis with an anti-HA antibody (HA-7; H9658, Sigma).
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed (20 mM Tris, 500 mM NaCl, 10 mM imidazole, pH 7, protease inhibitor, lysozyme, 250 units of Benzonase (Sigma)) and incubated on ice for 20 min ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Developmental Biology 2019Quote: ... cells were cultured from day 7 in induction medium supplemented with either 1mg/mL Heparin Sodium Salt (Heparin, Sigma-Aldrich), 0.25uM Valproic Acid (VA ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Cathepsin B was measured with the fluorogenic substrate Na-CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin hydrochloride (Sigma) after a 30-min pretreatment with DTT and EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... iPSCs were differentiated into iPSC-CMs using the Wnt modulation-differentiation method (Lian et al., 2012) with 7 μM CHIR (Selleckchem) and 5 μM IWP2 (Sigma) in RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then washed twice with PBS and treated for 7 minutes in freshly-prepared fluorescence quenching buffer (0.1% sodium borohydride (452882-25G, Sigma-Aldrich) in PBS) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 7-dichloro-8-hydroxyquinoline (CLCQ; D64600-Lot#STBH7389) and Zinc Chloride (ZnCl2; 208086-Lot#MKCL1763) were purchased from Sigma Aldrich. We prepared 10mM stocks solutions of the inhibitors in Dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... 10-15 days old astrocytic cultures were subject to a sublethal dose of H2O2 (0.7-1.5 mM) for 45 minutes and subsequently incubated in absence or presence of 500 uM L-carnitine (Sigma-Tau) for 2 hours ...
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bioRxiv - Immunology 2020Quote: Each peptide sample was desalted on Empore SPE C18 cartridges (standard density, bed inner diameter 7 mm, volume 3 ml; Sigma), then concentrated by vacuum centrifugation and reconstituted in 40 µl 0.1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes were resuspended at 1×10^7 cells/mL in complete DMEM and incubated in the presence of 0.1 µM dexamethasone (Sigma-Aldrich) at 37 °C for 18 h ...
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bioRxiv - Neuroscience 2021Quote: ... GTS1-3 tissue was mechanically dissociated with a scalpel in ice-cold GKN-BSA containing 11.1 mM D-(+)-Glucose monohydrate (14431-43-7, Sigma Aldrich) and 50 mM bovine serum albumin (BSA ...
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Systemic administration of Ivabradine, an HCN channel inhibitor, blocks spontaneous absence seizuresbioRxiv - Neuroscience 2021Quote: ... and ELA (N-[4-[2-(3,4-Dihydro-6,7-dimethoxy-2(1H)-isoquinolinyl)ethyl]phenyl]-9,10-dihydro-5-methoxy-9-oxo-4-acridinecarboxamide) were purchased from Sigma-Aldrich. For systemic injections ...
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved MSP1E3D1 was collected in the flow-through fraction and concentrated to 7 mg mL−1 in a 10 kDa MWCO centrifugation spin filter (Millipore).
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bioRxiv - Cell Biology 2022Quote: ... and differentiated at 4×106 cells per 40 mm dish for 7 days at 37 °C in RPMI media (Sigma) supplemented with 1 mM Sodium Pyruvate (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 100 mg fragments were incubated in a 12-well plate with M199 media ± 7 nM insulin (Actrapid, Novo Nordisk) and 25 nM dexamethasone (D4902, Sigma). After 24 hr incubation ...
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bioRxiv - Immunology 2022Quote: ... Plates were incubated at 37 degrees C in 5% CO2 for 7 days before fixing with 2% formaldehyde and staining with 0.1% crystal violet (Sigma-Aldrich).
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bioRxiv - Genetics 2022Quote: For Masson’s trichrome staining, frozen muscle (diaphragm, 7 mice/group) sections were stained in Weigert’s iron haematoxylin working solution (Sigma-Aldrich), Biebrich Scarlet-Acid fuchsin solution (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... starting day 7 post-transfection to select for the loss of Hprt and with 1 μM ganciclovir (GCV, Sigma PHR1593) to select against the LP plasmid backbone that contained a HSV1-ΔTK expression cassette ...
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bioRxiv - Molecular Biology 2022Quote: Estrogen and progesterone receptor-positive breast adenocarcinoma MCF-7 cell line (#HTB-22, ATCC) was cultivated in Dulbecco’s Minimum Essential Medium (DMEM, #D6429, Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... plants were fumigated for 30 min with 50 µM 2′,7′-dichlorofluorescein diacetate (H2DCFDA; Millipore-Sigma, St. Louis, MO, USA) for ROS imaging ...
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bioRxiv - Plant Biology 2023Quote: ... plants were fumigated for 30 min with 50 µM 2′,7′-dichlorofluorescein diacetate (H2DCFDA; Millipore-Sigma, St. Louis, MO, USA) for ROS imaging ...
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bioRxiv - Plant Biology 2022Quote: ... Sections of 7 to 8 mm were then transferred to slides treated with 100 mg/mL poly-L-Lys (Sigma) and dried on a warm plate at 40 °C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... pH of the nutrients medium was adjusted to 7 with NaOH and jelled with 0.7% (w/v) Agar (Sigma-Aldrich). In this study ...
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bioRxiv - Cancer Biology 2022Quote: For live cell imaging cells were seeded on glass bottom plates which were previously coated with either 1mg/ml fibronectin (CKPC cells) or 10 μg/ml laminin (COS-7 cells, #L2020, Sigma). The cells were imaged using a Zeiss 880 LSM with Airyscan microscope which has a 37°C humidified incubator and perfused with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Microbiology 2023Quote: ... The tPA fluorogenic substrate D-Val-Leu-Lys 7-amido-4-methylcoumarin (10 μM) (Millipore-Sigma, Burlington, MA, USA, #A8171) was added ...
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bioRxiv - Microbiology 2023Quote: ... The tPA fluorogenic substrate D-Val-Leu-Lys 7-amido-4-methylcoumarin (10 μM) (Millipore-Sigma, Burlington, MA, USA, #A8171) was added ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Cell Biology 2023Quote: ... Z slices of 250 nm were collected using 488 nm and 561 nm excitation lasers in the presence of a buffer containing 7 μM glucose oxidase (Sigma), 56 nM catalase (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Sorted cells were then combined with the non-enriched fraction at a 3:7 ratio and resuspended in cold PBS supplemented with 1% BSA (Miltenyi) and 0.5 U/μl RNase Inhibitor (Sigma Aldrich). Cells were partitioned into Gel Beads-in-emulsion (GEMs ...
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bioRxiv - Molecular Biology 2023Quote: ... One microliter of each reaction was run on a 7% SDS-PAGE gel and visualized by silver-staining (silver nitrate, Sigma). The rest of the reaction was subjected to SDS-PAGE followed by autoradiography ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The mineral suspensions were mixed with 0-200 ng/μL salmon sperm DNA in triplicates (also at pH 7) (Sigma). The mineral-DNA suspensions were incubated at room temperature for 16 hrs under constant shaking at 70 rpm ...
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bioRxiv - Bioengineering 2023Quote: Evaluation of fluid velocity of static and flow-conditioned vessels was assessed at day 7 through perfusion of 2.0 µm fluorescent beads (Fluorescent blue latex beads, Sigma Aldrich) into the microvascular networks using reservoirs with applied pressure gradients of 5 mm H2O ...
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bioRxiv - Immunology 2023Quote: Tumors were digested for 45 minutes with gentle shaking at 37°C in HBSS containing 7 mg/ml Collagenase D and 2 mg/ml DNase I (Sigma). Single-cell suspensions were stained with fluorophore-conjugated antibodies obtained from BioLegend ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the testicular and epididymal cross-sections were stained with 0.1% RB2 (catalog no. 12236-82-7; RB2; R115; Sigma-Aldrich) in 100 mM carbonate-bicarbonate buffer (pH 10 ...
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bioRxiv - Bioengineering 2023Quote: ... After selection for plasmid integration with 1 μg/ml puromycin for 5-7 days, cells were induced for effector (CasRx, DjCas13d or other Cas13d orthologs) expression using Doxycycline (D3072, Sigma). 3-5 days after induction ...
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bioRxiv - Developmental Biology 2023Quote: Oxidative stress was quantified by measuring total reactive oxygen species (ROS) using 2μM 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA; Sigma-Aldrich) staining and FACS analysis ...
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bioRxiv - Cell Biology 2023Quote: ... incubating them in 0.5 ml cFAD with 3.75×10^7 PFU/ml supplemented with 4 μg/ml Polybrene (Sigma Aldrich, H9268) on top of the insert and 2 ml cFAD on the bottom ...
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bioRxiv - Cancer Biology 2023Quote: ... T47D and MCF-7 cells were incubated in phenol red-free charcoal-stripped medium with E2 (10 nM; Sigma-Aldrich) for 5 min and 15 min/37°C ...
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bioRxiv - Immunology 2023Quote: Thymic lobes were isolated from 1- to 3-day old neonatal WT or RIP-mOVA mice and cultured for 7-10 days on a filter-strainer stack (filter: MF-Millipore Membrane Filter ...
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bioRxiv - Plant Biology 2023Quote: ... The 30 collected fractions were combined into 15 pools and desalted on C18 Cartridges (Empore SPE Cartridges C18 standard density, bed I.D. 7 mm, volume 3 ml, Sigma-Aldrich). Each fraction was concentrated by vacuum centrifugation and reconstituted in 40 µl of 0.1% (v/v ...