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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for 6 Chloro 5 methoxypyridin 2 amine hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Reduction/alkylation of cysteine residues was performed by addition of Tris(2-carboxyethyl)phosphine hydrochloride and chloroacetamide (Sigma-Aldrich) at final concentrations of 5 mM and 30 mM ...
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bioRxiv - Neuroscience 2019Quote: ... For imaging Alexa647 with long term pH stability at pH 7.8 we used reducing agent 2-mercaptoethylamine-hydrochloride (MEA, 100 mM, Sigma) in a 0.2 M sodium phosphate buffer and a oxygen-scavenging system (10% (wt/vol ...
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bioRxiv - Microbiology 2021Quote: On-bead digestion of the protein on the streptavidin beads was performed as in (Hubner et al., 2015): 2.5 µl of 100 mM Tris(2-carboethyl)phosphine hydrochloride (TCEP, Sigma) was added to a final concentration of 5 mM to the beads and disulfide bonds were reduced for 1 hour while shaking in a thermoshaker at 37°C to prevent settling of the beads ...
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bioRxiv - Genetics 2021Quote: ... The supernatant was transferred to a fresh tube and reduced with 0.5mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma) for 30 minutes at 60°C and alkylated in 3 mM methyl methanethiosulfonate (MMTS ...
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bioRxiv - Cell Biology 2022Quote: ... Disulphide bridges were reduced by the addition of 0.8 μM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma, cat. no. C4706). β-arrestin was labeled by incubation with 0.8 μM Alexa Fluor™ 647 C2 Maleimide (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... samples were mixed with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine hydrochloride (Cat. # C4706, Sigma Aldrich) and incubated at 57°C for 45 min ...
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bioRxiv - Biochemistry 2023Quote: ... followed by quenching of 50μL the deuterium exchange reaction mixture in 50μL pre-chilled 50mM sodium acetate quench solution containing 2 M guanidine hydrochloride (Sigma) and 200 mM TCEP ...
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.4) supplemented with 5mM 4-chloro-7-nitrobenzofurazan (NBF-Cl, Sigma-Aldrich), as described (Zivanovic et al 2019) ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein was also visualized using 4-Chloro-1-naphthol solution (Sigma Aldrich) which is a substrate for HRP in a reaction that results in a colored precipitate.
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bioRxiv - Pathology 2022Quote: ... samples in TRIzol were separated using bromo-chloro-propane (Sigma, St. Louis, MO). The aqueous phase was collected into a new tube and glycogen was added as a carrier ...
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bioRxiv - Cancer Biology 2021Quote: Sub-confluent cells were labelled with 25 µM chloro-deoxyuridine (CldU) (Sigma Aldrich) before media was removed and replaced with fresh media containing 250 µM iodo-deoxyuridine (IdU ...
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bioRxiv - Cancer Biology 2023Quote: ... The reaction was stopped using 10% Tri Chloro Acetic Acid (Sigma; Cat# T6399) and kept at 4°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... #22363548) in a 6 well plate containing 1.35 mM 2’-deoxyguanosine (2-dG, Sigma-Aldrich) in complete DMEM-10 (DMEM ...
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bioRxiv - Bioengineering 2021Quote: ... in 100 mM sodium phosphate - 5 mM ethylenediaminetetraacetic acid (EDTA) buffer (pH 6.5) with 10 mM L-cysteine–hydrochloride (all from Sigma-Aldrich) at 60 °C and 10 rpm for 18 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ketamine hydrochloride (50 mg/kg) and xylazine (5 mg/kg) (Koc et al., 2005) purchased from Sigma-Aldrich (Missouri, USA), then sacrificed and their colon tissues were quickly dissected ...
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bioRxiv - Biochemistry 2022Quote: ... Each mAb was diluted to a final concentration of 0.32 μg/μL with guanidine hydrochloride (Sigma, 5 M final concentration) and reduced into Lc and Hc with DTT (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... The infected HIE-18 lines were cultured in 75 cm2 flasks containing 12 ml of Schneider’s medium supplemented with 10% FBS at 28°C and subcultured every 5-6 days by splitting at a ratio of 1:2 into new flasks (Sigma Aldrich, NSW, Australia).
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Neuroscience 2021Quote: ... 5-6 dpf larvae were exposed to 10 μM neomycin (Sigma) in E3 for 30 minutes at 28°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM potassium ferricyanide [K3Fe(CN)6] (Sigma cat. #P-8131), and 1 mg/ ml X-gal [5-bromo-4-chloro-3-indolyl-β-D-galactoside] was made fresh ...
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bioRxiv - Cell Biology 2021Quote: ... 135 mM KCl] with 50 mM 5(6)-Carboxyfluorescein dye (Sigma) to form dye filled liposomes for permeabilization assays or NMR size exclusion buffer [20 mM HEPES (pH 6.8) ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4[Fe(CN)6]·3H2O (P-3289, Sigma-Aldrich), and 2 mM MgCl2 (M-8266 ...
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bioRxiv - Immunology 2023Quote: ... 10% POPG) filled with 50 mM 5(6)-Carboxyfluorescein dye (Sigma) were prepared as previously described29 ...
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bioRxiv - Neuroscience 2024Quote: ... One group of mice (referred to as DMI + 6-OHDA) was pre-treated with one injection of desipramine hydrochloride (DMI, Sigma-Aldrich, 25mg/kg i.p.) 30 minutes before the 6-OHDA infusion in order to protect the noradrenergic system [50].
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bioRxiv - Cancer Biology 2021Quote: ... 5×10−5 M 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), recombinant murine IL-3 (0.2 ng/mL) ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μM 5-fluoro-2’-deoxyuridine (Sigma Aldrich 200-072-5), penicillin ...
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bioRxiv - Biophysics 2020Quote: TIF2NRID was amine-labelled with a fluorescent probe (fluorescein isothiocyanate, FITC, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and 100-fold excess of the reducing agent hydroxyl amine (NH2OH) (Sigma) at 60 °C and room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) (Sigma). The reaction was allowed to proceed for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... Primary amine concentration was quantified using a standard ninhydrin assay (Sigma-Aldrich) performed according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and amine group by D-amino acid oxidase (Sigma Aldrich, United States). Horseradish peroxidase (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Amines were detected by spraying TLC plates with 0.2g Ninhydrin (Sigma-Aldrich) dissolved in 100ml ethanol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... N-diisopropyl ethyl amine were purchased from Sigma Aldrich (St. Louis, MO). HPLC grade acetonitrile was purchased from Supelco (Bellefonte ...
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bioRxiv - Neuroscience 2020Quote: ... in 5 mL of 2-methyl-2-butanol (Sigma Aldrich, #240486). Mice were deeply anesthetized using 15 µL of Avertin 2.5% per gram of body weight ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were washed once with 40 mL 0.1 mM 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF, Sigma-Aldrich A8456), transferred into conical 50 mL tubes and centrifuged at 1700 x g for 5 min ...
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bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
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bioRxiv - Cancer Biology 2020Quote: ... Polar metabolites were first methoximated with methoxyamine hydrochloride in pyridine (Sigma, 20 μl, 30 mg/ml, 2 hours, 37 °C) and then derivatized with TMS reagent (N,O-bis(trimethylsilyl ...
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bioRxiv - Cancer Biology 2020Quote: Dried and frozen metabolite extracts were derivatized with 50 µl of 2% (w/v) hydroxylamine hydrochloride in pyridine (Sigma-Aldrich) for 60 min at 90°C ...
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bioRxiv - Neuroscience 2020Quote: ... from Tocris CGP-55845 ((2S)-3-[[(1S)-1-(3,4-Dichlorophenyl)ethyl]amino-2-hydro xypropyl](phenylmethyl)phosphinic acid hydrochloride) from Sigma-Aldrich
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was transferred to a fresh tube and reduced with 1 mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma) for 30 minutes at 60°C and alkylated in 4 mM methyl methanethiosulfonate (MMTS ...
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bioRxiv - Physiology 2023Quote: ... Dried and frozen metabolite extracts were derivatized with 50 µl of 2% (w/v) hydroxylamine hydrochloride in pyridine (Millipore Sigma) for 60 min at 90°C ...
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bioRxiv - Cell Biology 2023Quote: ... Pellets were resuspended in 1 x Nupage LDS Sample buffer with either 50 mM DTT (followed by heated at 75 0C for 10 min) or with 10 mM Tris(2-carboxyethyl) phosphine hydrochloride (Sigma) (followed by incubation at room temperature for 2 hrs) ...
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bioRxiv - Biophysics 2023Quote: ... 5 % D2O (Eurisotop) and 5 mM DSS-d6 (2,2-dimethyl-2-silapentane-5-sulfonate, Sigma) as internal reference (Wishart and Sykes ...
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bioRxiv - Biochemistry 2023Quote: 2-Pyridinecarboxaldehyde (1) and 6-(1-piperazinylmethyl)-2-pyridinecarboxaldehyde bistosylate salt were purchased from Sigma-Aldrich. 5-Ethynylpicolinaldehyde (“alkyne-2PCA” ...
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bioRxiv - Neuroscience 2021Quote: ... Quantification of dopamine (DA) and serotonin (5-HT) was carried out by LC/MS using the following analytical standards: dopamine hydrochloride (D2960000: Sigma-Aldrich), dopamine-1,1,2,2-d4hydrochloride (73483 ...
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bioRxiv - Cell Biology 2019Quote: ... 5 μL of the serial dilutions were transferred from the 96-well plate onto YPD agar plates with or without additional 5 mM guanidinium hydrochloride (Sigma-Aldrich) and/or 50 ng/mL cycloheximide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Medium was removed 24 hours post-transduction and replaced with selective growth medium containing 5 μg /ml of Blasticidine S hydrochloride (Sigma-Aldrich). Selective medium was changed every few days then replaced with normal growth medium after 7 days ...
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bioRxiv - Developmental Biology 2022Quote: ... 2.5 and 5 μg/ml phenylhydrazine in fish water prepared from a freshly made 5 mg/ml stock solution of phenylhydrazine hydrochloride (Sigma-Aldrich). Non-treated fish served as control ...
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bioRxiv - Microbiology 2020Quote: ... 45 μL was then suspended in 5 μL of a 10 x stock of D-(+)-glucosamine hydrochloride (hereby referred to as GLCN, Sigma-Aldrich) or media to a final volume of 50 μL ...