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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 5′-chloro-2′-deoxyuridine (CldU) at 30 µM (Sigma-Aldrich) for 4 h ...
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bioRxiv - Cancer Biology 2020Quote: ... and active caspase-3 (1:1 000, Millipore, AB3623). Three fields of view for each slide were imaged using an Olympus BX43 Upright Microscope and cells were manually counted and scored using ImageJ Software.
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bioRxiv - Biophysics 2022Quote: ... 250 μM 3-isobutyl-1-1-methylxantine IBMX (Sigma), 100 nM Cortisol (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM 3-Isobutyl-1-methylxanthine (IBMX, Sigma) in PBS for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: Subcutaneous injections of 5-Chloro-2’-deoxyuridine (CldU) or 5-Iodo-2’deoxyurdine (IdU) (Sigma) were administered (50μg/g dissolved in saline ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10000; D9542, Sigma-Aldrich, St Louis, USA), and mounted on glass slides with Mowiol 4-88 (Merck Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF) and 1x PhosSTOP phosphatase inhibitor cocktail (Sigma-Aldrich), and centrifuged at 10,000 x g for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear DNA was stained with DAPI (4’,6-diamidino-2-phenylindole) (1 mg/ml) (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... The slides were then stained with 1/1000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 minutes at room temperature and washed twice for 5 minutes in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 min ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 µg ml-1 of 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich Chemie GmbH).
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bioRxiv - Cell Biology 2020Quote: ... with 1 μg/mL 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) protease inhibitor (Sigma-Aldrich, Inc.) according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2021Quote: ... paraformaldehyde (PFA) and 4-(2-hydroxyethyl)-1-piperazine-ethane-sulfonic acid (HEPES) were purchased from Sigma Chemical (St ...
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bioRxiv - Cell Biology 2022Quote: ... Cell nuclei were stained with 10.9 μM 4′,6-diamidino-2-phenylindole (DAPI, 1:1000 Sigma). Coverslips were mounted in ProlongGold (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was counterstained with 1 μg/mL DAPI (4’,6’-diamino-2-fenil-indol; Sigma-Aldrich) and mounted onto glass slides with 20 mM Tris pH8 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich, 1:1000).
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated in 4′,6-diamidino-2-phenylindole (1:1000, DAPI, Sigma-Aldrich, Cat # D9542) solution for 10 mins ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 min before being incubated with 1:1000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) and 1:200 Phalloidin Alexa-647 (Stratech ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated with 4’,6-diamidino-2-phenylindole (DAPI; 1:5,000; Merck Millipore) and covered with Mowiol (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... Cell nuclei were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich, 1:1000). For the DAPI staining ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Three more washing steps with TBS were performed before resolving the staining with 5 ml of substrate per membrane: a 30 mg tablet of 4-chloro-1-naphthol (Sigma) dissolved in 10 ml of methanol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell metabolites were dissolved in 0.5 mL of ice-cold 80% methanol solution containing 1 μg/mL 4-chloro-DL-phenylalanine (Sigma, C6506). The samples were then incubated for 30 min in a -80°C freezer ...
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bioRxiv - Immunology 2024Quote: ... 4% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate (CHAPS; Sigma-Aldrich, Cat. No. 226947)] ...
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bioRxiv - Developmental Biology 2022Quote: ... 2/3 mTeSR1 + 1/3 N2 medium + LSX + PSD Day 6,7: 1/3 mTeSR1 + 2/3 N2 medium + PSD At day 6 cells were detached by Accutase solution (Sigma-Aldrich) and incubated at 37°C for 20 minutes to obtain a single cell suspension ...
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bioRxiv - Cell Biology 2024Quote: ... two siRNA sequences targeting different regions of NOK were designed as #1: 5’-GCAAGAAACAUUCAUGCAU-3’ and #2: 5’-GUCUUUCCCAGGGACACAA-3’ (SASI_Hs01_00043410 and SASI_Hs02_00351937, Sigma, St. Louis, MO, USA). The control siRNA is a scrambled sequence (5’-UUCUCCGAACGUGUCACGUdTdT-3’ ...
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Intestine-to-neuronal signaling alters risk-taking behaviors in food-deprived Caenorhabditis elegansbioRxiv - Neuroscience 2021Quote: Copper sulfate gradients were visualized using 1-(2-Pyridylazo)-2-naphthol (PAN, Sigma 101036). Plates with 25 μL of 5 mM ...
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bioRxiv - Genetics 2023Quote: ... and rat monoclonal anti-laminin-2 (α2 chain; 4H8-2; Sigma Aldrich; 1:500). Secondary antibodies used were goat polyclonal anti-rabbit Alexa Fluor 594-conjugated (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 3-4 and 7-9 were treated with 100 µL of embryo water plus 1 µg/mL 2′,7′-Dichlorofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) in 0.4% DMSO ...
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bioRxiv - Molecular Biology 2024Quote: L-(−)-Nicotine and 4-(methylamino)-1-(3-pyridyl)-1-butanone dihydrochloride (pseudooxynicotine) were purchased from Sigma-Aldrich (USA) and Toronto Research Chemicals (Canada) ...
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bioRxiv - Biochemistry 2021Quote: ... cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, MO) as described previously.52 Equivalent MTT assays were performed on cells cultured in the same ratios of PBS to media ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 3 hours before addition of 30 μL of the bacterial growth reporter MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (2.5 mg/mL) (Sigma) and Tween 80 (10% ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed by 3-(4,5-1,2methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Merck Millipore). K562 cells (5,000/well ...
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bioRxiv - Cancer Biology 2023Quote: ... After that cells were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-.Aldrich) in a 1:10 ratio in a culture medium and incubated at 37 °C for 3 h ...
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bioRxiv - Cell Biology 2023Quote: Cell viability was determined by adding 0.5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) substrate to cells and subsequent incubation at 37 °C and 5% CO2 for 1–2 h ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability was assessed using colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay following by the manufacturer’s instructions (Sigma). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... and 2 g/L U-[2H] glucose (Sigma-Aldrich, St ...
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bioRxiv - Immunology 2022Quote: ... Perturbation profiling of the use of metabolic pathways by T cells was achieved by the addition of oligomycin (O4876; 1 µM), Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920; 2 µM) and rotenone (R8875; 1 µM - all from Sigma Aldrich, St. Louis, MO). Metabolic parameters were then calculated based on the following formulas:
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Biochemistry 2023Quote: ... The blots were probed with the following primary antibodies at room temperature for 1-2 hours or at 4 ℃ overnight: anti-GAPDH (Millipore, #MAB374, 1:5,000 dilution), anti-Flag M2 (Sigma ...
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bioRxiv - Zoology 2023Quote: ... All wells were then washed with embryo media three times before being incubated on an orbital incubator shaker at 130 rpm and 28°C for 15-20 minutes in media containing 0.01% 2-[4-(dimethylamino) styryl]-1-ethylpyridinium iodide (DASPEI, Sigma Aldrich; cat. #3785-01-1) to selectively stain live neuromast hair cells ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings at the one leaf stage were treated in 1/2 Hogland solution (pH 6.0) containing 1.5% NaCl (Sigma-Aldrich) or 20% polyethylene glycol (PEG ...
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bioRxiv - Microbiology 2021Quote: ... and transfer vector at 1:1:1 molar ratio using the X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich; # 6365787001) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid 3-azido-1-propanol ester chain transfer agent (CTA-azide) were purchased from Sigma-Aldrich (Macquarie Park, Australia). DBCO-modified Ang-2 peptide (TFFYGGSRGKRNNFKTEEYC(Mal-DBCO) ...
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Anisotropic Rod-Shaped Particles Influence Injectable Granular Hydrogel Properties and Cell InvasionbioRxiv - Bioengineering 2021Quote: ... The microparticles were collected into HFE-7500 containing 20 vol.% of 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma) to destabilize the surfactants ...
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bioRxiv - Molecular Biology 2024Quote: ... The recovered emulsion was then broken with 100 μL of 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma-Aldrich), and the aqueous phase was mixed with 100 μL of 200 μg/μL total yeast RNA (Ambion).
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bioRxiv - Bioengineering 2020Quote: ... Serial dilutions of the samples (1:2 to 1:64) and a standard row containing 1 to 40 nmol Neu5Ac (Sigma Aldrich) were prepared in extraction buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA, 0.5 mM DTT, 1 mM NaF, 1 mM PMSF and 1% Protease Inhibitor Cocktail from Sigma, pH 7.4) for 30 min on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were ground in an ice-cold buffer (50 mmol.L-1 TRIS, 5 mmol.L-1 EDTA, 2 mmol.L-1 DTT, protease inhibitor cocktail (Sigma, St. Louis, MO, USA)) ...
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bioRxiv - Immunology 2023Quote: ... The fresh tissue was dissected followed by digestion for 2 hours at 37°C in 1 mg ml−1 Collagenase 1 (Sigma-Aldrich). Debris was removed and cells isolated by sequentially passing through 100 ...
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bioRxiv - Developmental Biology 2023Quote: ... 90%, 100%), incubated in acetone/Spurr resin (1:1, 30 minutes, 1:2, 30 minutes) and in Spurr resin (Sigma-Aldrich) at room temperature (RT ...