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Citations for Millipore Sigma :
8851 - 8900 of 10000+ citations for UDP β L Arabinofuranose since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 500 mg/L BSA-FV and 8 mM Sodium-DL-Lactate (Sigma-Aldrich, St. Louis, MO, USA), for 4 days ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... and plated in 24-well plates on coverslips (Laboratory Glassware Marienfeld) coated with poly-L-lysine (Sigma) and laminin (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... The differentiation medium was the DMEM growth medium containing 50 μg mL−1 l-ascorbic acid (Sigma), and 10 mm β-glycerophosphate (Sigma) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The gelatinous sack surrounding the eggs was removed by incubation in 3% L-Cysteine (Merck Millipore, USA) while rotated by hand for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated either on 12 mm cover slides coated with 0.1mg/ml poly-L-ornithine(Sigma), or on µ-Slide 8 Well Glass Bottom (#1.5 polymer coating cat ...
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bioRxiv - Neuroscience 2023Quote: ... mice obtained food pellets containing 1.025% L-leucine-15N stable isotope (Sigma-Aldrich, St Louis, MN, USA) for 3 weeks between P28 and P49 (in parallel with PL/rhEPO injections) ...
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bioRxiv - Microbiology 2023Quote: ... coli strains was performed at 37 °C in LB (20g/L LB Broth (Lennox) (Sigma-Aldrich; L3022), 2xYT (tryptone 16g/L (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... The calli were selected on callus-inducing medium supplemented with Hygromycin B (50 mg/L; Sigma Aldrich) for two rounds (14 days each round) ...
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bioRxiv - Molecular Biology 2023Quote: ... and heparin (0.0002%) on plates coated in poly-L-ornithine (0.01%) and laminin (0.01 mg/mL) (Sigma). Lines were cultured to become differentiated glioma cells by adaptation to media of DMEM-F12 (Wisent ...
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bioRxiv - Immunology 2024Quote: Early-MM mice9 were pretreated for one week with antibiotic mix containing Ampicillin (1g/L, Sigma-Aldrich), Vancomycin (0.5g/L ...
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bioRxiv - Physiology 2024Quote: ... The coverslips were coated with poly- L-lysine to aid cell attachment (Sigma-Aldrich, St. Louis, MO).For the optogenetic system ...
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bioRxiv - Cell Biology 2024Quote: ... NAC (N-Acetyl-L-cysteine) and sodium iodoacetamide were purchased from Sigma Aldrich (catalogue # A9165 and GERPN6302).
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bioRxiv - Immunology 2024Quote: ... CHO ligand anchor cells (short and long) were maintained in L-Glutamine-free DMEM (Sigma-Aldrich #D6546) supplemented with 10% dialysed FBS (dialysed three times against 10 L PBS) ...
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bioRxiv - Genomics 2024Quote: ... 30 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich, #A8960-5G; reconstituted in PBS), 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2024Quote: ... DNP-Dpa is N-3-(2,4-dinitrophenyl)-L-2,3 diaminopropionic acid]) (Sigma Aldrich Cat. no. SCP0193-1MG) at a final concentration of 7.5 µM was added to the preincubated N-TIMP2:MMP solution ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were prepared by adding a drop of poly-L-lysine solution (0.01%) (Sigma-Aldrich - A-005-C) and incubating at room temperature for 30 minutes ...
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bioRxiv - Biophysics 2024Quote: Microfluid imaging chambers were constructed and coated with 15 µg/ml >300KDa Poly-L-Lysine (PLL, Sigma) on the day of imaging as described previously (Pilagov et al ...
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bioRxiv - Cancer Biology 2024Quote: Cells were treated for 72 h with glucose-free medium supplemented with 4.5 g/L galactose (Sigma) or 4.5 g/L glucose (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Cells from two brains were seeded in a Poly-L-Lysine-coated (15 μl/ml, Sigma-Aldrich) culture flask ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded at 45 000 cells/cm2 on coverslips coated with poly-L-lysine (Sigma-Aldrich). After 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... at 400,000 cells per well on double-coated [hESC-qualified Matrigel Matrix and Poly-L-Ornithine (Sigma)] 10 cm tissue-culture treated plates ...
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bioRxiv - Neuroscience 2024Quote: ... Corning 6-well cell culture plates were pre-coated with Poly-L-Ornithine (PLO, Sigma #P4957-50ML) and incubated for 1h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 15 g/L agar) plates or in low-salt Luria-Bertani Lennox broth (LB; Sigma-Aldrich L3022).
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bioRxiv - Physiology 2024Quote: ... for 1 hour followed by incubation with Hoechst (2 µmol/L, Cat. # 94403; Sigma-Aldrich, Darmstadt, Germany) for 10 minutes at room temperature ...
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bioRxiv - Physiology 2024Quote: HEK293 cells were seeded at low density onto coverslips pre-treated with poly-L-lysine (Sigma-Aldrich) and recorded using the whole-cell configuration of the patch clamp technique ...
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bioRxiv - Physiology 2024Quote: ... the dissected pieces of the cocoon were mounted on positively charged (X) Poly-L lysine (Sigma-Aldrich) coated slides and stained for 15 min covered from light with a staining solution containing propidium iodide and DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... that had previously been coated with poly-L-ornithine (Sigma-Aldrich, 0.1 mg/mL in borate buffer) overnight at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown in HL5 culture medium without glucose (Formedium) supplemented with 18 g/l maltose (Sigma) and 50 μg/ml ampicillin and 40 μg/ml streptomycin ...
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bioRxiv - Systems Biology 2024Quote: ... the resulting strains were pinned on SC-Leu+Ade plates (300 mg/l adenine (A8626, Sigma-Aldrich)) and grown for 1 day at 30°C before proceeding with fluorescence measurements using flow cytometry and storing the libraries as glycerol stocks at -80°C.
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bioRxiv - Neuroscience 2024Quote: ... larvae were anesthetized with 200 mg/l 3-aminobenzoic acid ethyl ester (MS-222) (Sigma-Aldrich, A5040) and embedded laterally in 1.2% low-melting-point (LMP ...
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bioRxiv - Systems Biology 2024Quote: ... on SC(MSG)-Leu/Arg/Lys/Ura + canavanine/thialysine plates (50 mg/l canavanine (Sigma-Aldrich, C1625), 50 mg/l thialysine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... were subsequently washed three times in kinase buffer (25 mM Tris-HCl pH 7.4, 100 mM NaCl, 10 mM MgCl2, 50 μg/ml bovine serum albumin [Sigma], 0/1mM EGTA, 0.1% β-mercaptoethanol). Beads (10 μl ...
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bioRxiv - Genomics 2020Quote: ... RRID:AB_2798177) overnight at 4°C or 1:10,000 dilution of monoclonal mouse anti-human β-actin (clone AC-74, Sigma-Aldrich, Cat. No. A2228, RRID:AB_476997) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (AppliChem GmbH, Germany) (and 135 μg/ml biotin (Sigma-Aldrich, St Louis, MI) for Sd expression) ...
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bioRxiv - Developmental Biology 2020Quote: ... Gentle pressure was applied to epididymis tissue using forceps and sperm were allowed to swim out during a 45-minute incubation in HTF + 0.75mM methyl-β-cyclodextrin (Millipore MR-070-D, Sigma C4555) at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... (Piperno and Fuller 1985)), mouse-anti-β-tubulin (E7; at 1:100; Developmental Hybridoma Studies Bank, University of Iowa) and rabbit-anti-Zwf1 (Sigma A9521; at 1:10,000), followed by goat-anti-mouse-680 (LI-COR 926-68070 ...
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bioRxiv - Immunology 2021Quote: ... [AppliChem, Cat. #A3452], 25 mM MgCl2 [Sigma, Cat. #M2670], 450 mM KCl [AppliChem, Cat. #A293], 0.8 mM β-NAD [Sigma, Cat. N1511], 60 mM (NH4)2SO4 [Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 100,000 x g (membrane fraction) centrifugations were suspended in 100 μL of 2X loading buffer (4% SDS, 10% β-mercaptoethanol, Sigma-Aldrich, 20% (v/v) glycerol ...
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bioRxiv - Microbiology 2020Quote: ... 1:2000 (SARS-1-S, MERS-S and SARS-2-S) or 1:5000 (229E-S)] and mouse anti-β-actin (Sigma-Aldrich, A5447, 1:5000). As secondary antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... The blot was then stripped and re-probed with a 1:5000 dilution of anti-β-Actin antibody (Sigma catalogue #A2066, St. Louis, MO, USA) in TBST with 1% milk ...
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bioRxiv - Neuroscience 2019Quote: The following drugs were used for behavioral pharmacology experiments: the non-selective β-adrenergic receptor (βAR) antagonist DL-propranolol hydrochloride (Sigma-Aldrich, St. Louis, MO), the α1AR antagonist prazosin hydrochloride (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... yeast cells were pre-cultured for 24h in SD–URA at 30°C and then shifted to SD-LEU medium supplemented with 1 µM β-Estradiol (Sigma- Aldrich, St. Louis, MO), incubated for 16-18h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Oocytes were mechanically homogenized and membrane proteins were extracted using DDM (100 mM phosphate buffer, 150 mM NaCl 0.5 % n-dodecyl-β-D-maltoside, SIGMAFAST Protease Inhibitor Cocktail (Sigma-Aldrich, St. Louis, MO, USA); pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... inhibition of CHIT1 enzyme activity was measured using fluorogenic substrate 4-methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside (4MU-GlcNAc3; Sigma-Aldrich, St Louis, MO) following hydrolysis measuring liberated fluorescent 4- in McIlvain buffer (100 mmol/l citric acid and 200 mmol/l sodium phosphate [pH 5.2] ...
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bioRxiv - Immunology 2022Quote: ... membranes were incubated with PBS-T or TBS-T complemented with 5 % BSA and primary antibodies specific for β-actin (Sigma-Aldrich, A5441, 1:2000), calnexin (Enzo Life science ...
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bioRxiv - Immunology 2022Quote: The primary and secondary antibodies that were used throughout our studies include the following: mouse anti-β-tubulin (1:5000; catalog # T8328-200ul, Sigma-Aldrich, St. Louis, MO), mouse anti-GAPDH (1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... homogenized in extraction buffer A (10mM Tris-HCl, 0.4M sucrose, 10mM MgCl2, 2mM PMSF, 5mM β-mercaptoethanol, 1µg/ml protease inhibitor (Sigma-Aldrich, catalog No. 200-664-3), incubated with gentle shaking for 20min ...
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bioRxiv - Developmental Biology 2024Quote: ... For each IVF session one epididymis from 12-week-old WT and one from Trim66-null were transferred into 90 μL of capacitation medium, consisting of TYH (Takeo & Nakagata, 2011) with 0.75 mM methyl-β-cyclodextrin (MBCD, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany). Spermatozoa were allowed to disperse from the tissue and incubated for 30 min in a 5% CO2 incubator at 37°C.
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bioRxiv - Immunology 2023Quote: ... we removed the head kidneys from RNAlater and placed them in a tube with ∼10 ceramic beads (1.4 mm) and 600 µL of RTL Buffer enriched with 6 µL β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA) to avoid sample degradation by RNases ...
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bioRxiv - Neuroscience 2023Quote: ... Enzymatic activity of beta-hexosaminidase (combined A and B isoforms) was determined by hydrolysis of 4-methylumbelliferyl-N-acetyl-β-glucosaminide (EMD Millipore Chemicals, Darmstadt, Germany) using 1.25 mM substrate in the incubation mixture ...