Labshake search
Citations for Millipore Sigma :
8851 - 8900 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... AtMPK4 and AtMPK6 was performed using anti-MAP kinase-antibody (1:20000 dilutions) as primary antibody and anti-mouse IgG (Sigma) protein (1:15000 dilutions ...
-
bioRxiv - Biochemistry 2019Quote: ... anti-actin (A4700) and anti-tubulin antibody (T6199) and goat anti-human IgG–HRP antibodies were obtained from Sigma-Aldrich, Dorset ...
-
bioRxiv - Genetics 2021Quote: ... 10 μg of chromatin was immunoprecipitated with the one of the following antibodies: 5 μg CTCF antibody (07-729, Merck-Millipore), 5 μg RAD21 antibody (Abcam ...
-
bioRxiv - Cell Biology 2021Quote: ... The membranes were stained with primary antibodies diluted 1:1,000 in TTBS followed by incubation with horse peroxidase-labeled secondary antibody (Millipore Sigma) diluted 1:10,000 in 2% non-fat milk in TTBS ...
-
bioRxiv - Biochemistry 2021Quote: ... Pierce anti-HA beads for immunoprecipitation of HA-tagged GAPDH was purchased from Thermo Fisher (Cat. # 88836) Rabbit polyclonal antibody (Cat. # G4595) and mouse monoclonal antibody against GAPDH (Cat. # G8795) were purchased from Sigma Aldrich. Rabbit polyclonal antibody against Peroxiredoxin-SO3 (Cat ...
-
bioRxiv - Molecular Biology 2021Quote: ... The immunoblotting was performed with the following primary antibodies (overnight at 4°C) and secondary antibodies (1h at RT): mouse-anti-Flag (M2 monoclonal, #F3165, 1:3000, Sigma), rabbit-anti-GAPDH (monoclonal ...
-
bioRxiv - Neuroscience 2020Quote: ... Anti-α-tubulin primary antibody as well as secondary antibodies coupled to horseradish peroxidase (HRP) were obtained from Sigma-Aldrich. AlexaFluor-labelled secondary antibodies were obtained from ThermoFisher ...
-
bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation reactions were performed using anti-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, F1804, Sigma-Aldrich). The complex of chromatin antibody was captured with protein G beads (Invitrogen) ...
-
bioRxiv - Plant Biology 2021Quote: ... Immunoblot of phosphorylated MAPKs was performed using anti-phospho p44/42 MAPK antibody as the primary antibody and peroxidase-conjugated goat anti-rabbit IgG (Sigma) as the secondary antibody.
-
bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
-
bioRxiv - Immunology 2021Quote: ... that utilizes the same human antibodies.12 Antibodies were prepared 1:500 in carbonate-bicarbonate coating buffer (SRE0034, Sigma-Aldrich), added to each well of a Costar 96-well Assay plate (3369 ...
-
bioRxiv - Microbiology 2021Quote: ... We just changed the secondary antibody and for this step horseradish peroxidase (HRP)-conjugated rabbit anti-bovine IgG antibody (Sigma) was used ...
-
bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were immunodetected using 1:2500 dilution of the rabbit anti-TbKH polyclonal antibody and 1:15,000 dilution of goat anti-rabbit-HRP antibody (Sigma-Aldrich). The chemiluminescent protocol was used for developing as indicated below.
-
bioRxiv - Neuroscience 2022Quote: ... blocked in PBST and 10% NGS for 2–3 hours at room temperature, and incubated in rat anti-mCherry antibody (1:2000, Life Sciences) and rabbit anti-TH antibody (1:1000, Millipore) at 4°C for four nights ...
-
bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with an alkaline phosphatase-conjugated secondary antibody directed against the first antibody (Sigma Aldrich, Taufkirchen, Germany) for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT ...
-
bioRxiv - Neuroscience 2022Quote: ... Mouse monoclonal antibodies against MAP2 (M4403, IC: 1:100) and mouse monoclonal antibodies against GAPDH (WB: 1:1000) were from Sigma. Mouse monoclonal antibodies against GAD65 (GAD-6 supernatant ...
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
-
bioRxiv - Cell Biology 2022Quote: ... A mouse anti-myosin heavy chain antibody was used as primary antibody (1:200; Sigma Aldrich, St. Louis, MO, USA) and Alexa 488 donkey anti-mouse IgG antibody as secondary antibody (Life Technologies ...
-
bioRxiv - Cell Biology 2022Quote: The mouse monoclonal anti-GABAA α1 subunit antibody (clone BD24) (catalog #: MAB339) and mouse monoclonal anti-GABAA β2/β3 antibody (catalog #: 05-474) were obtained from Millipore. The rabbit monoclonal anti-GABAA α1 subunit antibody (catalog # ...
-
bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-β-actin antibody (catalog #: A1978) and mouse monoclonal FLAG antibody (catalog #: F1804) came from Sigma Aldrich. The fluorescent anti-β-actin antibody Rhodamine came from Biorad (catalog # ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by incubation in 1:50 dilution of biotinylated anti-lucifer yellow antibody (RRID: AB_2536191) in universal antibody dilution solution (Sigma Aldrich) for 3 days at 4°C with occasional gentle agitation ...
-
bioRxiv - Cell Biology 2019Quote: ... or incubations with species specific biotinylated antibodies (Vector, 1:100), then streptavidin-HRP antibody (Vector, 1:200) before development with diaminobenzidine (Sigma). Counterstaining was performed using toluidine blue ...
-
bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibodies were suspended in antibody buffer containing 1% bovine serum albumin (BSA, Sigma Aldrich, cat. Number A9418) and 0.1% Triton X-100 in D-PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... Blotting was performed according to standard procedures using primary antibodies as follows: Two Lpd-specific antibodies were purchased from Sigma (HPA016744 recognising the N-terminus ...
-
bioRxiv - Microbiology 2021Quote: ... except that the primary antibody for viral detection was a monoclonal antibody against the coronavirus group antigen (Milipore Sigma #MAB9013) and the secondary was a horseradish-peroxidase-conjugated goat-anti-mouse antibody (enQuire BioReagents # Q2AB1).
-
bioRxiv - Genetics 2022Quote: ... then resuspended by 50μL antibody buffer (washing buffer plus digitonin 1000:1, EDTA, pH = 8.0, 2mM) with H3K4me2 antibody (Sigma Millpore 07030) diluted at ratio of 1:100 ...
-
bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
-
bioRxiv - Neuroscience 2019Quote: ... sections were incubated in 3% normal goat serum in PBS for 1h and incubated overnight at 4°C with the following primary antibodies: (1) rabbit monoclonal antibody against NeuN (1:1000, MAB377, Millipore), (2 ...
-
bioRxiv - Biochemistry 2019Quote: ... the presence of the viruses was revealed by immunostaining in infected cells with a polyclonal rabbit antibody that recognizes the MOPV Z protein (Agrobio, France) and a phosphatase alkaline-conjugated polyclonal goat anti-rabbit antibody (Sigma) and 1-Step NBT/BCIP substrate (Thermo Fisher scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... similarly performed as in co-immunoprecipitation except that the primary antibody used is anti-PCNA antibody (cat#SAB2108448; Sigma-Aldrich) in 1:750 dilutions.
-
bioRxiv - Cell Biology 2019Quote: ... gels were transferred onto 0.45 μm PVDF membranes using a semidry transfer apparatus before being subjected to immunoblot analysis using specific primary antibodies and secondary antibodies (Sigma). Protein detection was performed using ECL chemiluminescent reagent (GE Healthcare ...
-
bioRxiv - Cell Biology 2019Quote: ... Bound primary antibodies were detected using peroxidase-coupled secondary anti-rabbit antibody (7074, Cell signalling) and enhanced chemiluminescence (WBLUF0500, Millipore). Blots were exposed digitally using the ChemiDoc MP System (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2020Quote: ... for 1 hour at room temperature followed by incubation overnight at 4°C with indicated antibodies NFIB (HPA003956, rabbit antibody, 1:1000 dilution, Sigma); AR (ab74272 ...
-
bioRxiv - Cell Biology 2020Quote: ... washed three times with TBST and incubated with the primary antibody – the polyclonal anti-Dam1p (Keating et al., 2009) or monoclonal anti-GFP antibody (#11814460001 from Sigma) overnight at 4 °C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The primary antibodies were diluted in Antibody diluent (PBS with 0.3% Triton X-100 and 1% bovine serum albumin (A9647, Sigma Aldrich)) and applied to the sections overnight at 4 °C in a humidified chamber ...
-
bioRxiv - Microbiology 2021Quote: Isotyping of the monoclonal antibodies and polyclonal sera responses was performed using the Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich), according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... The following primary antibodies were used for the Western blotting: mouse antibodies against Flag (Sigma-Aldrich, St. Louis, MO, USA) and mouse antibody against β-actin (Proteintech Group ...
-
bioRxiv - Cell Biology 2020Quote: ... The target protein was detected by specific primary antibodies followed by secondary horseradish peroxidase (HRP)-conjugated antibodies (for less abundant proteins) (Sigma) or fluorescence-labeled secondary antibodies (for abundant antigens ...
-
bioRxiv - Plant Biology 2021Quote: ... GSTU5 was detected by the anti-GST primary antibody (Novex, 1:500) and anti-mouse secondary antibody (Sigma, 1:5000).
-
bioRxiv - Immunology 2020Quote: ... Immunofluorescence staining using a goat anti-LYVE1 antibody (R&D) followed by incubation with an Alexa647-conjugated secondary antibody together with Hoechst33342 (Sigma) was performed on 7 µm sections without acetone fixation ...
-
bioRxiv - Neuroscience 2021Quote: ... IHC-1:250, no. MAB5374), and Anti-polyglutamine (poly-Q) antibody (WB-1:5000, no. P1874) antibodies were obtained from Millipore-Sigma ...
-
bioRxiv - Physiology 2020Quote: ... Brains were then preincubated in 5% normal goat serum in PBT for 3 hours at room temperature with gentle agitation followed by incubation in 1:50 dilution of biotinylated anti-lucifer yellow antibody (RRID: AB_2536191) in universal antibody dilution solution (Sigma Aldrich) for 3 days at 4°C with occasional gentle agitation ...
-
bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated with primary antibodies specific for TSC22D4 (home-made, by PINEDA Antibody Service, Flag-HRP (#A8592, Sigma), HA (#2999 ...
-
bioRxiv - Cancer Biology 2020Quote: ... serum samples used to detect levels of IgG by anti-IgG-HRP antibody (Bio-Techne) or IgM by anti-IgM-HRP antibody (Sigma) on 100μg/ml NP-KLH-coated 57 ...
-
Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... RPN11- 3xFLAG tagged subunits were detected by monoclonal anti-FLAG antibodies (Monoclonal ANTI- FLAG® M2 antibody #F3165, Millipore Sigma). The lysate from a wild-type strain with untagged RPN11 was used as a negative control ...
-
bioRxiv - Neuroscience 2022Quote: ... syp-mCh and SSF-tagged opioid receptors using the lipofectamine method were incubated for 15 minutes with Alexa-647 conjugated anti-FLAG antibody (1/1000, M1 antibody from Sigma, Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher ...
-
bioRxiv - Microbiology 2022Quote: ... samples were washed with 1 ml PBS buffer three times and vacuum-infiltrated for 20 min with 0.1 mg/ml of primary mouse anti-1,3-β-glucan antibody (Biosupplies; Sydney, Australia) for β-1,3-glucan labelling or 0.1 mg/ml mouse MOPC-104E primary antibody (Sigma-Aldrich) for α-1,3-glucan labelling ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Secondary antibody HRP conjugated-rabbit anti-goat IgG (cat#: AD106P) and primary anti-MPO antibody (cat#: AF3667) were purchased from Sigma and R&D systems ...
-
bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 1 h with a 1:5000 dilution of the secondary antibody (anti-rabbit IgG horseradish peroxidise-conjugated antibody, Sigma) in antibody buffer and then washed three-times for 15 min in TTBS ...