Labshake search
Citations for Millipore Sigma :
8751 - 8800 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... and goat anti-mouse IgG antibody HPR conjugate (1:10,000 EMD Millipore, 12-348; 12-349). Western blot detection was done with ChemiDoc XRS+ System-Bio-Rad and quantified using ImageJ software.
-
bioRxiv - Cancer Biology 2024Quote: ... The primary antibodies and concentrations are as follows: mouse anti-Flag (Millipore Sigma F3165, 1:1000), rabbit anti-LHPP (Millipore Sigma ...
-
bioRxiv - Neuroscience 2024Quote: Mouse brains were homogenized in PBS containing 1% Triton X-100 and protease inhibitor cocktail (Sigma) and incubated on ice for 30 min ...
-
bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated with an antibody recognizing the neoepitope of CSPGs (mouse, 1:50000, Millipore), anti-NCAN (sheep ...
-
bioRxiv - Developmental Biology 2024Quote: ... The primary antibody used was a mouse anti-FLAG M2 monoclonal antibody (1 : 2000, Sigma, F1804). The secondary antibodies used were Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody ...
-
bioRxiv - Immunology 2024Quote: Luminex cytokine assay was performed utilizing the Mouse Cytokine/ Chemokine Magnetic Bead Panel (Millipore# MCYTOMAG-70K). Reagents were prepared as per kit instructions (including wash buffers ...
-
bioRxiv - Biophysics 2024Quote: ... and anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma), revealed with donkey anti-mouse secondary antibodies conjugated to Alexa Fluor 555 ...
-
bioRxiv - Cell Biology 2024Quote: The fixed chips were first stained with mouse anti-CD31 (PECAM-1, 1:100; Sigma-Aldrich) solved in antibody diluent made of 3% Bovine Serum Albumin (BSA ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were immunostained with mouse anti-TOMM20 antibody (1:100 dilution, clone 4F3, Sigma-Aldrich #WH0009804M1), rabbit anti-LAMP1 antibody (1:100 dilution ...
-
bioRxiv - Molecular Biology 2024Quote: ... supplemented with 1:10000 dilution of anti-mouse alpha-Tubulin (Clone B-5-1-2; SIGMA) for 1 to 3 h at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Staining was performed against glial fibrillary acidic protein (mouse anti GFAP−Cy3™, Sigma-Aldrich, C9205), Secondary antibody was anti-mouse Alexa Fluor 488 (Thermo-Fisher ...
-
bioRxiv - Biophysics 2024Quote: ... COS-7 cells were labeled with primary antibodies (mouse anti-β-tubulin, 1:600, T4026, Sigma; rabbit anti-Tom20 ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated in secondary antibodies (Goat anti-mouse CF633; Sigma-Aldrich; catalog #SAB4600139; 1:1000) diluted in 1% normal goat serum and 1% fish gelatin in 1% PBSTw overnight in 4° C ...
-
bioRxiv - Neuroscience 2024Quote: ... for 1 hour then incubated with anti-α-synuclein 5G4 antibody (1:50,000, mouse, Millipore MABN389) in 2% milk in PBS with 0.3% triton X-100 at room temperature overnight ...
-
bioRxiv - Cancer Biology 2024Quote: ... The Atto 647N conjugated Goat anti-mouse-IgG was purchased from Sigma-Aldrich (catalog number 50185). DAPI (catalog number H21486) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein samples were transferred to nitrocellulose membranes (Amersham) upon which M2 Anti-FLAG mouse antibodies (Sigma) were used to visualize 3X-FLAG fusion peptides.
-
bioRxiv - Immunology 2024Quote: ... 20 ng/mL recombinant mouse GM-CSF (Miltenyi-Biotec) and 0.5 μM ß-estradiol (Sigma-Aldrich) and transduced with ER-HoxB8 retrovirus using Lentiblast Premium (OZ Biosciences).71 After two weeks ...
-
β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... adding the wells with peroxidase-conjugated secondary anti-mouse IgG antibodies (Sigma-Aldrich, diluted 1:1,000) and incubating for 1h at ambient temperature ...
-
bioRxiv - Immunology 2024Quote: ... the cells were stained with mouse anti-myeloperoxidase (MPO) antibody (1:500, #MA1-80878, Sigma-Aldrich) for 1 h at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... using rabbit PLA plus and mouse PLA minus probes and red Duolink detection reagents (Sigma-Aldrich). Finally ...
-
bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells transfected with the pSpCas9(BB)-2A-Puro plasmid (sgRNAs 1) were treated with 1 μg/mL puromycin (Sigma-Aldrich, 58-58-2) 24 hours after transfection and were selected for 96 hours ...
-
bioRxiv - Immunology 2022Quote: ... coli transformed with plasmid pET42 expressing N-terminally GST- and HIS-tagged human IL-18 were grown at 37°C in Lysogeny broth (LB) medium containing kanamycin (25 μg/ml) as selection marker for the pET42a plasmid (Cat No 70561, Novagen, Merck, Overijse, Belgium). When the optical density at 600 nm (OD600 ...
-
bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
-
HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: Nef and LMP7 genes were cloned into pGEX-6P-1 (GE, Boston, MA, USA) and pET-28a plasmids respectively (Novagen, Madison, WI, USA). pGEX-6P-1 empty plasmid and pEGX-6P-1-Nef plasmid were respectively transformed into BL21 (DE3 ...
-
bioRxiv - Immunology 2020Quote: ... monoclonal antibodies were produced by transient co-transfection of 293-F cells with human heavy chain and light chain antibody expression plasmids using polyethylenimine (PEI) (Sigma-Aldrich, catalog #408727). Seven days after transfection ...
-
bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
-
bioRxiv - Biophysics 2022Quote: ... were cultivated to 85% confluency in 35 mm culture dishes and transfected with 1.0 μg plasmid DNA using polyethyleneimine (Sigma, St. Louis, MO, USA). After 12 h at 37 °C in 5% CO2 ...
-
bioRxiv - Cell Biology 2022Quote: ... from NIH3T3 cells co-expressing Flag-NDRG1 WT or S336A plasmids with GFP-Cdc42 were incubated with 30 μl of ANTI-FLAG® M2 Affinity Gel (Sigma-Aldrich, A2220) and eluted with 3X FLAG® Peptide (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2022Quote: ... coli DH10B (remaining plasmids) were used as cloning strains and electroporated with desalted ligation/Hot Fusion reaction (MF-Millipore membrane, VSWP, 0.025 μm).
-
bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 5 µg of EPS15 or DECR1 expression plasmids using X-tremeGENE™ 9 DNA Transfection Reagent (Sigma-Aldrich, 6365787001) using 1:3 DNA:reagent ratio ...
-
bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
-
bioRxiv - Molecular Biology 2023Quote: Two-fold dilution series from 40 to 1.25 plasmids per 5µl were prepared using sterile filtered water for molecular biology (Sigma-Aldrich, Saint-Quentin Fallavier, France). Eight repeats of the six dilutions were analyzed per plate ...
-
bioRxiv - Immunology 2023Quote: Chitinase activity in the culture supernatant of CHIT1 plasmid-transfected HEK293T cells was measured by using a fluorescence assay kit (Sigma-Aldrich Cat. # CS1030). A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate ...
-
bioRxiv - Genetics 2023Quote: ... WT WTC11 hiPSCs were co-transfected with the pC13N-iCAG-dCas9-VPR-P2A-mCherry repair template and pZT-C13-L1 and pZT-C13-R1 plasmids using GeneJuice transfection reagent (Sigma; cat. no. 70967). Edited hiPSCs were selected using G418 (InvivoGen ...
-
bioRxiv - Plant Biology 2023Quote: ... Expression of the plasmid in floral tissue of T2 plants was confirmed by GUS staining assay following the manufacturer’s protocol (Millipore Sigma, Burlington, MA, USA) (Figure S6) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Individual cell clones were picked and transfected with pKH011-puro guide plasmids together with piggyBAC transposase and selected with 2 μg/mL Puromycin (Millipore Sigma, Cat# 540411). Cells were subsequently maintained with 6 μg/mL Blasticidin and 2 μg/mL Puromycin ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were transfected with siRNAs/plasmid as described earlier and RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit (Sigma Aldrich, # RTN70). RNA was reverse transcribe using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher #4368814) ...
-
bioRxiv - Biochemistry 2021Quote: ... followed by TBST-Blotto containing two primary antibodies: monoclonal mouse anti-FLAG M2 (1:2,000 dilution, Sigma) and monoclonal rabbit anti-RNA polymerase beta (1:2,000 dilution ...
-
bioRxiv - Biophysics 2019Quote: ... Primary antibodies against Z01 (mouse monoclonal IgG1 Thermo Scientifique 33-9100) and Vangl1 (rabbit Sigma Aldrich HPA025235) were diluted at 1/200 and 1/500 respectively in blocking medium and incubated 45min at RT ...
-
bioRxiv - Cancer Biology 2021Quote: ... Homogeneous loading and transfer were evaluated using β-actin antibodies (Sigma, Clone AC15, Mouse, 1:10,000 dilution). Membranes were labelled with the appropriate IRDye conjugated secondary antibody (LI-COR ...
-
bioRxiv - Cell Biology 2020Quote: The following commercial antibodies were used in this study: anti-β-actin (mouse; Sigma, A1978; RRID: AB_476692), anti-emerin (rabbit ...
-
bioRxiv - Microbiology 2020Quote: ... Slides were subsequently stained for mesenchymal lineage cells using a mouse anti-vimentin (Sigma; Cat. No. HPA001762), at a 1:1000 dilution in antibody diluent overnight at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with either HRP-linked goat anti-mouse IgG antibody (dilution 1:10,000; Sigma-Aldrich) or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000 ...
-
bioRxiv - Cell Biology 2020Quote: ... We then incubated cells in serum free DMEM with 1/1000 mouse anti-Flag antibody (Sigma F1804) for 60 min at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: Commercially available polyclonal (pAb) or monoclonal (mAb) antibodies were used for Tubulin (Mouse mAb; Sigma, [DM1A] T6199), Plk1 (Mouse mAb ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 μg/ml prolactin (mouse recombinant for qPCR, western blot, immunohistochemistry and contraction control; Sigma or Peprotech) or sheep pituitary prolactin for time-lapse and confocal imaging ...
-
bioRxiv - Immunology 2021Quote: ... Binding serum antibodies were detected using HRP-conjugated anti-mouse IgG (Cat #A9044, Sigma Aldrich, 1:10,000), IgG1 (Cat #1070-05 ...
-
bioRxiv - Genetics 2021Quote: ... Covance #048) (Ouyang et al., 2013) and mouse anti-α-Tubulin (1:5,000 working dilution; Sigma #T6074).