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Citations for Millipore Sigma :
8551 - 8600 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cas9-sgRNA vector and a control vector (pEGFPN1) in the ratio: 2:1:1 using PEI (18) from Sigma Aldrich, Inc ...
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bioRxiv - Developmental Biology 2021Quote: Embryos used for long-term imaging or imaging after 1 dpf were treated with 0.003% 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) to prevent melanin pigment formation.
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bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
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bioRxiv - Cell Biology 2020Quote: ... For AsiSI or I-PpoI nuclease induced DSBs, cells were treated with Shield-1 (1 μM, AOB1848, Aobious) and 4-OHT (2 μM, H7904, Sigma) for 5 hours before washing with PBS and adding back media.
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bioRxiv - Plant Biology 2021Quote: ... 1 g of the infiltrated leaf tissue was ground in 4 mL of extraction buffer (1/2 tablet of Sigma Complete™ mini protease inhibitor (per 15 mL) ...
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bioRxiv - Microbiology 2021Quote: Test sera were heat-inactivated at 56°C for 30 minutes to destroy any complement activity and serially diluted 1:2 in cell culture media containing 1% foetal calf serum (Sigma). Virus was diluted to give 100-250 foci in the virus-only control and then added to the serum dilutions before incubation for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... then added to cells and incubated for 2 hours before discarding and adding a 2.4% (w/v) microcrystalline cellulose and 2% FCS (mixed 1:1) overlay (Sigma-Aldrich). The assay was incubated at 37°C and 5% CO2 for 70 hours then fixed in 4% PFA for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were rinsed in de-ionized water and re-fixed in freshly made Phosphomolybdic/Phosphotungstic/dH2O (1:1:2) acid solution for 8 min (#HT153, #HT152, Sigma), and then they were stained in Aniline Blue solution for 5 min (#HT154 ...
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bioRxiv - Developmental Biology 2022Quote: ... to induce brain regional patterning, EBs were treated individually with SAG (1:10,000) (Milli- pore, 566660) + IWP-2 (1:2,000) (Sigma-Aldrich, I0536) for ventral identity and with cyclopamine A (1:500 ...
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bioRxiv - Biochemistry 2020Quote: ... These were mounted in a drop of in 0.1 M phosphate buffer containing 1:1 of 2% methyl cellulose (Sigma-Aldrich) and 2.3 M sucrose on TEM grids with a carbon-coated Formvar film (Taab Laboratory Equipment Ltd) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EDTA and 2 mM PMSF) and eluted with 1 mg/ml FLAG® Peptide (Sigma, cat. no. F3290) in wash buffer overnight on a rotator at 4°C for anaylsis by mass spectrometry.
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Developmental Biology 2021Quote: ... 70nm Sections were picked up using a 1 :1 mixture of 2.3M sucrose and 2% methyl cellulose (cat# M-6385, Sigma-Aldrich) as a pick-up solution and sections were transferred to carbon and formvar coated mesh grids (cat# H100-Cu ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated with rabbit anti-TMEM43 polyclonal antibody (1:100, NBP1-84132, Novus) (Ab1-2) and mouse anti-FLAG (1:500, F1804, Sigma) for overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 1 - 2 µl of solutions containing 0.1 or 1% of the non-ionic detergent Triton X-100 (TrX; Sigma-Aldrich), dissolved in 0.1 M phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were treated with 1 x 10-5 M 5-fluoro-2-deoxy-uridine and with 1 x 10-5 M uridine (Sigma) for 3 days ...
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bioRxiv - Molecular Biology 2023Quote: ... These cultures were then sub-cultured (1:100 dilution) in 2 mL of TYG medium containing doxycycline (0.016 µg mL-1, Sigma-Aldrich), tetracycline (0.05 µg mL-1 ...
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bioRxiv - Immunology 2022Quote: ... murine lung and liver tissue samples were transferred into 2 ml tubes containing 1 mL of digestion media (1 mg/mL Collagenase D (Sigma 11088882001 ...
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bioRxiv - Microbiology 2022Quote: ... was previously isolated on the International Streptomyces Project 2 medium (ISP2, dextrose 4 g.L-1 [Sigma-Aldrich, St. Louis, MO, USA], yeast extract 4 g.L-1 [Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in appropriate secondaries diluted in 2% bovine serum in PBS for 1 h at 20-25°C and counterstained with Hoechst 33257 (1:1000; Sigma). For brain tissue incubated in anti-INO80 antibody (ab118787 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1/2.5/1 (v/v/v)) containing 10 µl of 2-isopropylmalic acid (0.5 mg/ml, Sigma-Aldrich, Tokyo, Japan) as an internal standard (IS) ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of each culture was removed and mixed 1:1 with freshly made 2% formaldehyde in PBS (Sigma-Aldrich) and stored at 4°C for analysis by flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of each culture was removed and mixed 1:1 with freshly made 2% formaldehyde in PBS (Sigma-Aldrich) and stored at 4°C for analysis by flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... the coupling of the PEG-acetal linker with HPV16 or fcHPV16 PsV particles was performed by immersing the PEG-coated cantilevers (∼1-to-2) in (w/v) 1% citric acid (Sigma) in milli-Q water for 10 mins ...
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bioRxiv - Plant Biology 2023Quote: ... fresh sporangia produced after crossing T0 Mprop mutants to wild-type (Tak-1 or Tak-2) were sterilised in 1 % (w/v) sodium dichloroisocyanurate (Sigma) solution for 3 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-MBP (1:500; Serotec) antibodies (OPCs) or 2 h incubation with mouse anti-GFAP (1:200, Sigma) (astrocytes) ...
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bioRxiv - Molecular Biology 2023Quote: ... the seeds were sowed on half strength Murashige and Skoog (1/2 MS) media with 0.8% sucrose and 1% Phytagel (Sigma-Aldrich) and kept the plates in the dark at 4 °C for 2 days for stratification treatment ...
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bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...
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bioRxiv - Biochemistry 2023Quote: ... Flow chambers were prepared with the following steps: 1) incubated for 2 min with block containing 25 μl of 1% Pluronic F-127 (Sigma), 50 μg/ml casein in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Uniprot P50750-1) and Cyclin T1 (residues 2-259; Uniprot O60563-1) were synthesized and cloned into pFastBac dual (Sigma). Each open reading frame codes for N-terminal 6-His tags and TEV cleavage sites ...
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bioRxiv - Systems Biology 2024Quote: ... Routine cells passaging was carried out at a 1:6 ratio every 2 days using 1× Trypsin–EDTA solution (Sigma) at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... They were then sowed on half strength Murashige and Skoog (1/2 MS) media containing 0.8% sucrose and 1% Phytagel (Sigma-Aldrich), followed by a stratification treatment at 4 ℃ in the dark for two days ...
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bioRxiv - Microbiology 2024Quote: ... α-tubulin was detected as a loading control with the mouse monoclonal antibody B-5-1-2 (Sigma, T5168, 1/4000) for 2 hrs at 25°C ...
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bioRxiv - Cell Biology 2024Quote: ... GTM3L cells were cultured in DMEM with 1% FBS and 1% PSG and exposed to 2% Polyethylene glycol 300 (PEG300; Sigma) for 2 or 5 days.
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bioRxiv - Plant Biology 2024Quote: ... and the pellets were frozen in liquid N2 before being re-suspended in 350 µl of lysis buffer (50mM Tris pH 6.8, 2% SDS, 1 mM DTT, 1 mM PMSF, 1X cOmplete protease inhibitor without EDTA – Sigma Aldrich) and incubated at 37 °C for 30 min with strong agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was stained with a freshly prepared 1:1 (vol/vol) mixture of 2% K4Fe (CN)6 (ferrocyanide or Prussian blue; Sigma) and 2% 11.6 M HCl (resulting in a final HCl concentration of 0.116 M ...
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bioRxiv - Biochemistry 2024Quote: ... Bacterial and fungal samples were resuspended in a ratio of 1:2:1 cell pellet: lysis buffer:acid-washed glass beads (Sigma #G8772). Mammalian cell lines and T ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with avertin (2, 2, 2-tribromoethanol, Sigma Aldrich, St. Louis, MO) or isoflurane for stereotaxic surgery (Model 1900 Stereotaxic Alignment System ...
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bioRxiv - Immunology 2023Quote: ... 10 µM N-acetylcysteine (NAC) or 2 mM 2-desoxyglucose (2-DG, both Sigma) were added during cultivation ...
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bioRxiv - Immunology 2023Quote: ... After general anesthesia with i.p.-administered Avertin (2, 2, 2-tribromoethanol, Sigma-Aldrich #T48402) the blood was collected with heparinized capillaries (Fisher Scientific™ #22-260950 ...
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bioRxiv - Biochemistry 2023Quote: ... Irgacure 2959 (2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone) were purchased from Sigma Aldrich for preparation of crosslinked polymer.
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 mM 2-Deoxy-D-glucose (Sigma-Aldrich), and 1 mM Sodium azide ...
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bioRxiv - Biophysics 2022Quote: ... 0.1% 2-mercaptoethanol (60-24-2, Sigma-Aldrich), and 0.1% leukaemia inhibitory factor (LIF ...
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bioRxiv - Plant Biology 2022Quote: ... 2 mM CaCl2 + 2 mM EGTA (Sigma MFCD00004291), 500 μM MgCl2 in transparent flasks ...
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bioRxiv - Immunology 2022Quote: ... 2-Amino-2-norbornanecarboxylic acid (BCH; Sigma, #A7902), α-(Methylamino)isobutyric acid (MeAIB ...
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bioRxiv - Immunology 2022Quote: ... 100 mM 2-deoxyglucose (2-DG) (Sigma-Aldrich). Non-glycolytic acidification of media (ECAR following injection of 2-DG ...
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bioRxiv - Neuroscience 2024Quote: ... 2-methyl-2-thiazoline (2MT; M83406, Sigma Aldrich) presented in a Plexiglas arena (43 × 27 × 19 cm ...
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-ME (Sigma 60-24-2). Cell Stimulation Cocktail (Tonbo # TNB-4975-UL100 ...