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Citations for Millipore Sigma :
8501 - 8550 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... α-Smooth Muscle antibody (A5228, SIGMA).
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bioRxiv - Molecular Biology 2024Quote: ... and M2 Flag antibody (A8592, Sigma). Western blot data for in vitro binding assays were analyzed using ImageJ software ...
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bioRxiv - Microbiology 2024Quote: ... Anti-FLAG antibody (Sigma-Aldrich, F1804) and allophycocyanin (APC)-conjugated anti-human IgG Fc antibody (Jackson ImmunoResearch,109-136-098 ...
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bioRxiv - Genomics 2024Quote: ... A mouse anti-FLAG antibody (Sigma) was used to detect spike protein expression.
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bioRxiv - Cell Biology 2019Quote: ... the captured proteins were eluted by 20 µl 2× SDS-PAGE loading dye and detected by western blot using anti-Flag antibody (Sigma, 1:3000, Cat# F1804).
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bioRxiv - Immunology 2021Quote: ... the membranes were washed in PBS-T for 40 min and the bound proteins were visualized with anti-FLAG-HRP antibodies (1:10000 in PBS-T for 1h, Sigma-Aldrich, Cat. no: A8592). All steps were performed at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with one or more of the following primary antibodies overnight at 4°C: AGO2 1:1500 dilution (Sigma-Aldrich SAB4200085 Lot#087M4841V), GW182 1:10,000 dilution (Bethyl A302-329A-T Lot#A302-329-T-2) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then collected on magnetic beads and resuspended in 50 µl Antibody Buffer (Wash buffer with 0.05% Digitonin and 2mM EDTA) containing one of the following antibodies in 1:100 dilution: H3K4me3 (Millipore 05-1339 CMA304, Lot 2780484), H3K27me3 (Cell Signaling #9733S C36B11 ...
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bioRxiv - Neuroscience 2019Quote: ... One mid rostro-caudal IC section from each animal was immunolabelled for GABA (rabbit anti-GABA antibody 1:1000, Sigma-Aldrich Cat# A2052, RRID:AB_477652), and the neuronal marker NeuN (mouse anti-NeuN antibody 1:1000 ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... The membranes were washed three times with PBS (5 min each time) and were then incubated with secondary peroxidase-conjugated antibody (1:15,000; Sigma-Aldrich, St. Louis, MO, USA). The antibody-bound proteins were detected using Pierce ECL western blotting substrate (Thermo Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... and heated to 90°C.[8] The samples were run, transferred, and probed with 1:500 dilution of primary antibodies (ALIX, CD9, TSG101 (Millipore Sigma, Burlington, MA, USA), calnexin (Cell Signaling Technology Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... antibody was used as a pan-neuronal marker and membranes were reprobed with mouse monoclonal anti-β-actin antibody (1:10,000; Sigma, Saint-Quentin Fallavier, France) to confirm equal protein loading ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins bound to beads were resolved on an SDS-PAGE and the presence of Nck was determined by immunoblot analysis using anti-Nck antibody (Millipore #06-288; 1:1000). As a negative control anti-Grb2 (BD Transduction #610112 ...
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bioRxiv - Cell Biology 2021Quote: ... the hiPSC-CMs were fixed and permeabilised with the FIX and PERM™ Cell Permeabilization kit and labelled with an antibody specific for α-actinin (1:250, Sigma-Aldrich #A7811), followed by an Alexa Fluor 350-conjugated secondary antibody (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... diluted anti-YFP antibody or other specific antibodies followed a 1:10,000 (v/v) diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or a goat anti-mouse antibody (Sigma-Aldrich, St. Louis, MO, USA). The detection signal was developed using the ECL substrate kit as instructed (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Proteins from WCLs and exosomes were resolved and imaged by SDS-PAGE and western blot analysis as described above using antibodies for FLAG (Sigma, F-1804; 1:5000), Alix (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-mouse HRP labeled secondary antibody (cat no: A9044; used at 1:5000 dilution for western blotting analysis) were obtained from Sigma-Aldrich (St. Louis, MO). Anti-rabbit secondary antibody Alexa 555 labeled (cat no ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Cell Biology 2022Quote: ... The proteins in the bound (B) and unbound (UB)/flow through were analyzed by Western blotting using anti-GST rabbit polyclonal antibody (Sigma #G7781, 1:5000 dilution).
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bioRxiv - Cell Biology 2022Quote: ... transferred to a nitrocellulose membrane, and probed with the following antibodies: anti-FLAG M2-HRP (HRP, horseradish peroxidase) conjugate (Sigma, A8592; dilution 1:10,000), rabbit anti-dynein heavy chain DYNC1H1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in blocking solution with the following primary antibody: 1:1000 mouse anti S-100 β subunit (S2532, Sigma-Aldrich, St. Louis, MO). Sections were washed and then incubated for 2h in blocking solution with the following secondary antibody at a 1:500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... Immunostaining was performed in cryostat sections (14 µm) using the mouse monoclonal anti FLAG M2 antibody (1:500; Ref F1804; Sigma-Aldrich, St Louis, Missouri) and the anti Sp8 C18 antibody (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... the cells were incubated for 1 h in the dark and at RT with 30 μL drops containing secondary antibody and DAPI (Sigma, 10 236 276 001) diluted 1:1000 in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... catalog # BP151-500) in PBS for 1 hour followed by overnight incubation at 4°C with the γ-H2AX antibody (Millipore Sigma, catalog # 05636I) using a 1:150 dilution and the H3K9me3 antibody (Millipore Sigma ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were blocked with 5% milk in Tris buffered saline (TBS) and probed with antibodies to HCMV IE1 and IE2 (Merck Millipore; MAB-8131; 1/5000), pp52 (Santa Cruz Biotechnology ...
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bioRxiv - Physiology 2019Quote: ... Secondary antibodies (see Table S1) were used at 1:500 dilution in 0.1% Tween-20/PBS with DAPI (Sigma-Aldrich, St. Louis, MO, USA). Samples were treated in the secondary antibody solution at 4°C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... 40 μm sections of PFA-fixed mouse brains were stained with the following antibodies: mouse anti-Gad67 (cat. no.: MAB5406; Millipore; 1:1000; RRID: AB_2278725); chicken anti-GFP (cat ...
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bioRxiv - Neuroscience 2019Quote: The level of RET phosphorylation in the cells was analyzed by Western blotting of immunoprecipitated RET with phospho-tyrosine-specific antibody (1:1500 Merck Millipore Cat# 05-321, RRID:AB_309678) as described previously (Leppanen et al. ...
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bioRxiv - Cell Biology 2019Quote: ... All mutations were confirmed by Sanger DNA sequencing and expression was confirmed by western blotting with anti-Myc primary antibody (1:2000 dilution; Merck Millipore #06-340, Billerica, MA).
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bioRxiv - Pathology 2020Quote: ... and 0.1% Triton-X 100 in PBS) and stained overnight at 4° C with antibodies against PRG2 (1:1000 dilution, rabbit; Sigma-Aldrich cat. no. HPA038515), HLA-G (1:10,000 dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in primary antibody overnight at 4° C (chick α-GFP, Abcam, Cat# AB13970, Lot# GF305729-1; mouse α- γ-tubulin, Sigma-Aldrich, Cat# T6557 ...
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bioRxiv - Microbiology 2019Quote: ... Blots were probed with the following antibodies diluted 1:5000 in 1X TBS-T containing 5% nonfat dry milk: rabbit anti-FLAG (Sigma-Aldrich Cat# F7425, RRID:AB_439687), mouse anti-FLAG (Sigma-Aldrich Cat# F1804 ...
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bioRxiv - Microbiology 2020Quote: ... Periplasmic and cytoplasmic fractions were separated electrophoretically using SDS-PAGE gels after which Western blotting was performed according to standard protocols using the following antibodies: anti-PhoA (Millipore MAB1012 diluted 1:2,500), anti-mouse-HRP (Abcam ab6789 diluted 1:5,000) ...
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bioRxiv - Developmental Biology 2020Quote: ... Standard IF staining was carried out by incubation of tissue sections with primary antibodies overnight at 4 °C at the following dilutions: mouse anti-acetylated tubulin (Sigma cat. # T7451; 1:1000), mouse anti-RAB11 (BD Transduction Laboratories cat ...
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2020Quote: ... Infected cells were also fixed and stained as above except that a post-fixation step using 100% methanol for 5 min was included and an OmpB antibody (11) and Hoechst (Sigma, B2261, 1:2500 dilution) was used instead of the anti-Rickettsia I7205 antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 hours at room temperature and incubated in the primary antibodies against anti-acetylated α-tubulin (mouse monoclonal, 1 mg/ml, Sigma Cat #T7451, USA), diluted in the blocking solution (2 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... One to three 12 μm sections were chosen randomly from both sides for staining as previously established6 using an anti-CGRP antibody (1:5000, Sigma C8189, anti-rabbit polyclonal), an anti-P2X3 antibody (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... The parafilm was washed with water and blocked with 1x phosphate-buffered saline (PBS) containing 3% (w/v) BSA and then incubated in α-HA antibody (Sigma; 1:1.500 dilution) diluted in PBS buffer and 3% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... at 20°C overnight and then treated with the secondary antibody (AlexaFluor 488-conjugated goat anti-mouse IgG; Sigma-Aldrich; 1:1000 in PBS) at 20°C overnight ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 5 μg of sheared chromatin were then used per immunoprecipitation with 1 μg of the following specific histone antibodies: anti-H3K4me3 (Millipore, cat. No. 07-473), anti-H3K4me1 (Diagenode ...
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bioRxiv - Cell Biology 2022Quote: ... The rabbit TgG monoclonal antibody for the detection of cleaved Notch1 (Val1744) (D3B8) (Cell Signalling Technology, cat. no. 4147) was diluted 1:200 in 1x Duolink Antibody Diluent (Sigma Aldrich, cat. no. DUO92002). The cells were incubated with the primary antibody overnight at 4°C and washed at room temperature three times for 5 min each with 1x Duolink In situ Wash Buffer A (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Neurons were stained with the following primary antibodies overnight at 4°C: mouse monoclonal anti-β-III-Tubulin (Tuj1, 1:1000; Millipore cat. No. MAB5564) and rabbit polyclonal anti-SARS-CoV-2 (COVID-19 ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled RNAs in the eluate were then detected by autoradiography and proteins were subjected to WB using an anti-Flag antibody (Sigma-Aldrich F3165; 1:10000).
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bioRxiv - Microbiology 2022Quote: ... the culture medium containing S-VSV were pre-treated to neutralize any residual G*-VSVΔG-GFP by incubating with anti-VSV-G antibody (Millipore, Cat #: MABF2337, 1:1,000 dilution) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for at least 20 hours at room temperature with a combination of the following primary antibodies in PBS-TX with 2% NGS or NDS: guinea pig anti-VGLUT1 (1:1000; AB5905, Sigma-Aldrich, St. Louis, USA), guinea pig anti-VGLUT2 (1:20,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were then incubated with the following primary antibodies diluted 1:200 in PBS overnight at 4°C: β-spectrin non-erythrocyte (hpa012685, Sigma, St. Louis, MO), collagen type II (ab34712 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then blocked for 3 hours in PBST and 5% normal goat or donkey serum at room temperature after which they were incubated in primary antibodies (rabbit anti-GABA 1:500, Sigma-Aldrich Cat# A2052, RRID:AB_477652) for at least 72 hours at 4°C ...