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Citations for Millipore Sigma :
801 - 850 of 7897 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... apoptotic cell death in 1-day old zebrafish whole-mounts was detected according to a modification of the ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Merck Millipore) protocol ...
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bioRxiv - Microbiology 2020Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Physiology 2021Quote: ... For the RT-qPCR the Kappa Mix (Sigma) was used and the primers concentration was 10 µM ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilized at RT with 0.25% TritonX100 (Sigma #T9284) for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 mg Fast Blue RT (Sigma-Aldrich) in 100 ml of 50 mM 2-amino-2-methyl-1,3-propanediol buffer (pH 9.8) ...
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bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
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bioRxiv - Synthetic Biology 2021Quote: ... Green fluorescence images were used for the detection of sfGFP and red fluorescence images were used for the detection of the sulforhodamine B dye (230162, Sigma-Aldrich), used to detect the correct flow of inputs.
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bioRxiv - Cancer Biology 2020Quote: Reverse transcription (RT) reactions were carried out using 1 µg of RNA using Transcriptor RT enzyme (Catalog No. 03531287001) from Sigma Aldrich. IR minigene PCRs were performed as previously reported ...
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bioRxiv - Developmental Biology 2021Quote: ... Up to 16 epiblasts were pooled per 600µL of Accutase (Sigma-Aldrich) for dissociation into single cells prior to staining ...
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bioRxiv - Molecular Biology 2019Quote: ... supernatant was incubated with protein A+G magnetic beads (Millipore 16-663) for 1h for preclearing at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and stained with 16 μg/mL propidium iodide (Sigma-Aldrich cat#287075) with 0.25 mg/mL Ribonuclease A for 30 mins at room temperature ...
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bioRxiv - Immunology 2021Quote: ... or 16 nM of phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich). After the 2h incubation period ...
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bioRxiv - Molecular Biology 2020Quote: ... The Nsp10/16 heterodimer was supplemented with 2 mM SAM (Sigma Aldrich) and concentrated at 21°C to 4.0 mg/ml using a 30 kDa cut-off centrifugal concentrator Amicon Ultra-15 (Millipore) ...
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bioRxiv - Microbiology 2022Quote: ... for 16 h and 5 mM adenosine triphosphate (ATP, # A26209, Sigma-Aldrich) for the time required for the analysis (1 to 8 hours) ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation at 16 162 xg (Sigma 1-14, Sigma Laborzentrifugen GmbH) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation at 16 162 xg (Sigma 1-14, Sigma Laborzentrifugen GmbH) for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... STR (0, 0.64, 2, 3.2, 16, 80, 400 μg/ml, Sigma S9137) or indicated combinations ...
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bioRxiv - Microbiology 2022Quote: ... Doxycycline for a Tet-off strain [16] was purchased from Sigma-Aldrich Chemical Co ...
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bioRxiv - Immunology 2022Quote: ... 12 and 16 after electroporation and transferred to TRI Reagent (Sigma-Aldrich). Combinatorial tonic signaling screens were performed in a similar way (harvest day 4 and day 16) ...
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bioRxiv - Cell Biology 2023Quote: ... OCT sections (16-μm thick) were then permeabilized in 0.5% Triton (Sigma) and 0.2% Tween (Sigma #P1379 ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl Magna ChIP™ Protein A Magnetic Beads (Sigma, 16-661) and antibodies (1 µg per 1 million-cell chromatin ...
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bioRxiv - Biophysics 2024Quote: ... cells were incubated overnight (∼16 h) with 2 mM thymidine (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with the anti-MPM2-Cy5 (EMD Millipore, 16-220), diluted in PBS (1:200) ...
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bioRxiv - Cancer Biology 2024Quote: ... for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125) for LONP1 immunoprecipitation on a rotating platform at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The linear constructs were gel purified using a PCR cleanup kit (Sigma-Aldrich, Australia), confirmed by Sanger sequencing and concentrated to ∼500 ng/μl.
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bioRxiv - Microbiology 2019Quote: ... The digests were then purified with either GenElute PCR Clean-Up Kit (Sigma-Aldrich) or QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... DNA from tail tips was isolated using Extract-N-Amp Tissue PCR kit (Sigma) and PCR genotyping undertaken as described (The Jackson Laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed with a commercial kit (Sigma JumpStart™ Taq ReadyMix™, P2893) and 1-μL of purified DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines tested negative for mycoplasma using LookOut Mycoplasma PCR Kit (Sigma, MP0035). All cells were maintained at 37°C in a humidified incubator with 5% CO2 and were grown in media supplemented with 10% dialyzed fetal bovine serum (Sigma-Aldrich F0392) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Annealed fragments were purified using the GenEluteTM PCR Clean-Up Kit (Sigma Aldrich, NA1020). Bi-directional precursor construct (pZ073 ...
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bioRxiv - Genetics 2023Quote: ... Amplification of the tagmented DNA was performed using the KAPA2G Robust PCR kit (Sigma) and custom P5 and P7 indexing primers ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was purified with RedExtract-N-Amp tissue PCR kit (XNAT-1000RXN, Sigma-Aldrich) using directions provided by the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted using the REDExtract-N-Amp Tissue PCR Kit protocol (Sigma-R4775) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... to quantify protein vicinity in HeLa cells and in neurons on coverslips as indicated in figures were performed using the Duolink In Situ Detection Reagents Orange kit (Sigma-Aldrich, DUO92007). Cells were fixed ...
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bioRxiv - Genomics 2021Quote: ... Alkaline phosphatase testing for the assessment of pluripotency before and after auxin treatment was performed using the Alkaline Phosphatase Detection Kit (EMD Millipore, SCR004).
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bioRxiv - Developmental Biology 2020Quote: Terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) was performed on frozen retinal cryosections using the ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA). TUNEL staining was performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: The in-situ interaction of NICD and active β-catenin was determined using the Duolink™ II secondary antibodies and detection kits (Sigma–Aldrich ...
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Multiple steps mediate ventricular layer attrition to form the adult mouse spinal cord central canalbioRxiv - Developmental Biology 2019Quote: ... TUNEL assay was performed on 20 μm thick cryosections using ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, Cat. # S7100) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed for 1 min in 4% formaldehyde and stained for AP activity using the Alkaline Phosphatase Detection Kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biochemistry 2019Quote: The detection of apoptotic cells was based on the nick-end (TUNEL) labelling method using the ApopTag® in situ detection kit (S7100, Merck Millipore, USA), according to the manufacturing protocol.
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bioRxiv - Cancer Biology 2019Quote: ... TUNEL staining was performed on deparaffinized sections to detect late-stage apoptotic cells using the ApopTag peroxidase in situ apoptosis detection kit (Millipore, Burlington, MA), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed using an ApopTag® In Situ Peroxidase Detection Kit (S7100, Merck Millipore, USA) according to the manufacturer’s recommendations and as previously described (36) ...
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bioRxiv - Cancer Biology 2020Quote: ... and processed on ABI 3500Xl Genetic Analyzer and mycoplasma testing was conducted with the Venor™ GeM Mycoplasma Detection Kit (Cat# MP0025-1kt, Sigma-Aldrich) both were done as recent as 1/9/2020 ...
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bioRxiv - Cell Biology 2021Quote: ... No contamination of mycoplasma was tested in every batch using the VenorGeM Mycoplasma Detection Kit (SIGMA-Aldrich MP-0025, St.Louis MO, USA), following the procedure indicated by the provider ...
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bioRxiv - Cancer Biology 2020Quote: Detection of an interaction between BRCA1 and the cavin or CAV1 proteins was assessed using the Duolink™ II Detection Kit (Sigma Aldrich) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2021Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using the ApopTag® Peroxidase in situ Apoptosis Detection Kit (EMD Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) was performed on frozen retinal cryosections using the ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA). TUNEL staining was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: The number of apoptotic cells were determined using the In Situ Cell Death Detection Kit (Sigma-Aldrich, 11684795910, St. Louis, MO, USA) in paraffin-embedded tissue sections ...
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bioRxiv - Physiology 2020Quote: ... TUNEL staining was performed using a protocol modified from the ApopTag peroxidase in situ apoptosis detection kit (Merck-Millipore, Cat no. S7100). Paraffin sections of adipose tissues were prepared as above ...
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bioRxiv - Microbiology 2022Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining of paraffin-embedded sagittal sections was performed using Apoptag Peroxidase In Situ Apoptosis Detection Kit (EMD Millipore, S7100), per the manufacturers’ instructions ...