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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected with TMB/E HRP substrate (Millipore Sigma, ES001) and 1 N HCl was used to stop the reaction ...
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bioRxiv - Neuroscience 2022Quote: ... and unspecific binding was blocked with TBS containing 10% goat serum (Millipore) or donkey serum (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... nonspecific binding was determined by the coaddition of 10 μM atropine (Sigma). The following day ...
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bioRxiv - Microbiology 2023Quote: ... The capsid binding inhibitor PF74 was purchased from Sigma-Aldrich (CAT# SML0835). The HIV-1 protease inhibitor Saquinavir (ARP-4658 ...
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bioRxiv - Neuroscience 2023Quote: ... for D2 binding and 20 nM mianserin hydrochloride (Sigma, cat#M2525, Canada) with 1 µM SCH-39166 hydrobromide (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with anti-Flag binding beads (Sigma, M8823) at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and filtered using 0.22 μm low-binding Durapore PVDF membrane filters (Millipore). 3RCF and 4RCF tau proteins were further purified by FPLC using size exclusion Superdex-75 and Superdex-200 columns (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... The column was equilibrated with binding buffer [100 mM sodium phosphate (Sigma), 150 mM NaCl (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: Ligand binding to commercial wild type HSA (non-defatted, Sigma-Aldrich A1653) and to HSA1 variant (His-tagged ...
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bioRxiv - Molecular Biology 2023Quote: ... binding was visualized using 3-amino-9-ethylcarbazole (AEC) (Sigma-Aldrich, Germany) and slides were counterstained using hematoxylin.
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bioRxiv - Genetics 2023Quote: ... The generic Cas12a binding region was designed and ordered from Sigma-Aldrich®.
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bioRxiv - Microbiology 2024Quote: ... goat ionized calcium binding adaptor molecule 1 antibody (anti-Iba1,1:500; Millipore), and mouse myelin basic protein antibody (anti-MBP ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein staining solution (REVERT Total Protein Stain kit, LI-COR Inc., # 926-11010; Pyronin Y (Sigma-Aldrich # P9172)
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bioRxiv - Cell Biology 2021Quote: ... The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
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bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cultures were stimulated with 20 ng/ml PMA for 3 hours and assessed with a mouse IFN-γ ELISA kit (Sigma-Millipore). For airway epithelial cell culture ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected and serum insulin levels were determined in triplicate using a rat/mouse insulin Enzyme-Linked Sorbent Assay (ELISA) kit from Millipore (EZRMI-13K) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... Phosphorylated ERK was measured by preparing epithelial cells as described above (Colon epithelial cell isolation) and using a Phospho-Erk1 (pThr202 / pTyr204 + Erk2 (pTyr185/187) and pan-Erk1 / 2 ELISA Kit from Sigma (Cat. RAB0349) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Plasma concentrations of acylated ghrelin and leptin were determined by commercial ELISA kits according to the manufacturers’ instructions (Spi Bio #A05117.96 and Millipore #EZML-82K, respectively).
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bioRxiv - Bioengineering 2021Quote: ELISpot plates (Millipore IP Filter plate) were coated with 20 µg/mL RBD in sterile PBS overnight at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ELISpot plates (Millipore IP Filter plate) were coated with 20μg/mL Spike in sterile PBS overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... ELISpot plates (Millipore IP Filter plate) were coated with 20 µg/mL heat-inactivated uricase in sterile PBS overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: The PLA assay was performed to determine the in situ binding between LC3 and ATG9a or ATG9b by using the Duolink® In Situ Red Starter Kit purchased from Sigma Aldrich (catalog number DUO92101), following the protocol provided by the manufacturer.
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bioRxiv - Microbiology 2019Quote: ... The supernatant was collected avoiding to disturb the pellet and was mixed with 750 microliters of Binding buffer of Total Spectrum RNA kit (Sigma–Aldrich, Saint Louis, MO, USA). Subsequently the extraction proceeded following manufacturer instructions.
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bioRxiv - Physiology 2020Quote: ... follicle stimulating hormone (FSH)/luteinizing hormone (LH) (ELISA, EMD Millipore), AMH (ELISA ...
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bioRxiv - Immunology 2022Quote: ... 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma) was added and after 20 minutes the reaction was terminated by adding Stop Reagent for TMB Substrate (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Pramlintide was quantified by ELISA (Merck Millipore, cat # EZHA-52) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: Total ATP content in a pool of 5 oocytes was determined using the Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit (FLASC, Sigma) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was finally visualized by a 0.1M PB solution containing 5% 3,3’-diaminobenzidine (DAB) (Sigma, Germany) and 0.02% H2O2 ...
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bioRxiv - Neuroscience 2020Quote: ... The protein solution was reconstituted into mesophase using molten monoolein spiked with 5% (w/w) cholesterol (Sigma) in 2:3 ratio of protein solution ...
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bioRxiv - Neuroscience 2020Quote: ... The protein extracts were run on a 5%–12% gel and transferred to nitrocellulose (NC) membranes (Millipore). After blocking in a milk solution (5% powdered milk in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were incubated overnight with 5 µL of Flag or V5 antibody-conjugated beads (Sigma-Aldrich) or with 1 µl of p50 antibody together with 20 µl of Protein A magnetic beads (Life Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to nitrocellulose membranes before blocking with 5% milk (Marvel) or bovine serum albumin (Sigma) w/v in PBS-T (0.1% Tween-20 in PBS) ...
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bioRxiv - Bioengineering 2022Quote: ... supernatant protein was removed by acidification with 5 g L-1 trichloroacetic acid (Supelco from Sigma-Aldrich), incubation for 20 min in room temperature and centrifugation at 20,238 x g for 10 min in a table-top centrifuge (Eppendorf 5424 ...
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bioRxiv - Molecular Biology 2023Quote: ... magnetic protein A/G beads were prepared with 5 µg of rabbit anti-AGO2 (Millipore, #03-110) or normal rabbit IgG antibodies (from the Magna RIP kit) ...
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bioRxiv - Immunology 2023Quote: ... protein samples were dried and reconstituted in 40 µl of 10% formic acid/5% DMSO (Sigma-Aldrich). Samples were analyzed as previously described [Van Herwijnen 2016] with a QExactive Plus instrument (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cas9 protein (CP01, PNAbio) was reconstituted in nuclease-free water and 5% Phenol Red Solution (Sigma Aldrich), following the guidelines in (71) ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins were loaded in the Laemmli sample buffer supplemented with 5% 2-mercaptoethanol (Sigma, catalog #: M3148) to reduce the disulfide bonds ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Cancer Biology 2024Quote: ... and up to 100 µg of protein per sample were reduced with 5 mM dithiothreitol (Sigma Aldrich) and incubated in the dark for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Cancer Biology 2022Quote: An in situ proximity ligation assay was performed to identify protein-protein interactions in cells using a Duolink In Situ Red Starter Kit (Sigma) per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein concentration in the samples was measured using the Bicinchoninic Acid Protein Assay Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Immunology 2023Quote: ... The protein concentration in the supernatant was measured by the BCA protein assay kit (Sigma-Aldrich, cat. No. BCA1-1KT).
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bioRxiv - Developmental Biology 2021Quote: ... Plates were sealed by piping a border of vaseline and 5% (v/v) mineral oil (Sigma, #M841-100 ml) and covered with a 22×22 Fisherbrand Cover Glass (# 12-541-B) ...
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bioRxiv - Neuroscience 2022Quote: ... and then plated into 24-well (1.8 x 10 5 cells per well) plates coated with 50 μg/ml poly-d-lysine (Sigma). After removal of the coating solution ...
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bioRxiv - Neuroscience 2019Quote: ... brains were dissected on an ice-cold plate in modified Schneider’s medium (SMactive) (71) with an addition of 5 mM Bis-Tris (Sigma) and then mounted on a glass-bottom dish (35 mm MatTek petri dish ...
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bioRxiv - Bioengineering 2020Quote: ... 48-well culture plates were coated with 5% w/v Pluronic™ F-127 (Sigma-Aldrich, St. Louis, MO) to make the surface of the tissue culture plastic non-adherent to cells [30] ...