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Citations for Millipore Sigma :
801 - 850 of 3834 citations for Recombinant Human AKT3 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... lysates were clarified and Flag-tagged protein were precipitated using anti-Flag M2 magnetic beads (Sigma Aldrich). After several washes in IP buffer ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were purified using anti-HA affinity matrix (Sigma; rat anti-HA clone 3F10 antibodies) and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged RtcB proteins were affinity-purified from the lysates with anti-Flag M2 affinity-gel (Sigma) followed by extensive washes ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed three times and FLAG-tagged proteins eluted using 3X FLAG peptide (Sigma; cat. F4799). Rhodamine-Azide labeling reactions were performed using 1.7 mM TBTA ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were detected with horseradish peroxidase (HRP)-conjugated anti-FLAG antibody (Sigma Aldrich, A8592-1MG). All western blots were developed using the ECL Plus Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged Arc was immunopurified using 50 µL of anti-FLAG M2 Magnetic Beads (Sigma-Aldrich, M8823) while endogenous TNIK was pulled-down with 10 µL of anti-TNIK antibody (BD Biosciences ...
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bioRxiv - Plant Biology 2020Quote: ... The FLAG-tagged PCRK2 and PBL19 proteins were immunoprecipitated using 15 μl M2 beads (Sigma; Cat. #A2220). Biotinylation was detected with Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Plant Biology 2021Quote: ... The FLAG-tagged NRG1C and SAG101 proteins were immunoprecipitated using 20 µl M2 beads (Sigma; Cat. #A2220) for co-immunoprecipitation and biotinylation was detected by Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... coli EF-G (704 aa, C-terminally His6-tagged) cloned into pET24a+ plasmid (Novagen, kanamycin resistance vector) was transformed into an E ...
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bioRxiv - Microbiology 2020Quote: ... HA3-tagged proteins were detected using a monoclonal rat α-HA3 (1:1000; clone 3F10; Sigma-Aldrich). Actin ...
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bioRxiv - Microbiology 2021Quote: ... FLAG®-tagged MprF was detected both by a mouse anti-FLAG® primary antibody (Sigma-Aldrich) and goat antimouse secondary antibody (LI-COR ...
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bioRxiv - Biochemistry 2022Quote: ... and HA-tagged full length CASK proteins were incubated with 1μg anti-Flag M2 antibody (Sigma, F3165) at 4°C over night ...
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bioRxiv - Cell Biology 2022Quote: Flag-tagged proteins were captured from 1 mg total cell lysate using anti-Flag affinity matrix (Sigma) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged zebrafish Grk1a or Grk1b was isolated using anti-FLAG M2 Affinity Gel (#A2220) from Millipore-Sigma as described previously (15 ...
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bioRxiv - Plant Biology 2021Quote: ... HA-tagged proteins were detected with an Anti-HA-Peroxidase antibody (Sigma-Aldrich, St. Louis, USA, 12013819001). For the RACB ubiquitination screening ...
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bioRxiv - Biochemistry 2021Quote: ... Flag-tagged H2B was purified by anti-Flag immunoprecipitation with ANTI-FLAG® M2 Affinity Gel (Millipore) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: PTP-tagged protein expression was demonstrated by immunoblotting with a rabbit polyclonal anti-protein A antibody (SIGMA) as described previously [13] ...
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bioRxiv - Biochemistry 2020Quote: ... for mFc tagged antigen versions or goat-anti-hIgG(Fc) conjugated with HRP (1:70000, A0170, Sigma) if hFc-tagged antigens had to be detected ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation of Flag-tagged proteins were carried out with the anti-FLAG M2 beads (Cat. A2220, Sigma) in RIPA buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3). ∼0.2 μg of GST-OsBZR1 ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma, #SAB4301135). Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... Flag-tagged proteins were detected using the anti-Flag HRP-coupled antibody (mouse monoclonal, A8592; Sigma-Aldrich) diluted 1:5000 (v/v) ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Genetics 2019Quote: Localization of tagged proteoforms in embryos was visualized by immunohistochemistry using Mouse Anti-FLAG M2 (F1804 Sigma), followed by biotinylated goat anti-Mouse IgG (BA-9200 ...
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bioRxiv - Immunology 2022Quote: ... Cells were treated with EGFP tagged esiRNA that served as an NC (NC-esiRNA-EGFP, Sigma-Aldrich). About ...
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bioRxiv - Cell Biology 2022Quote: ... Immunodetection of ProteinA (ProtA)-tagged proteins was performed using Peroxidase Anti-Peroxidase Soluble Complex antibody (Sigma-Aldrich) in a 1:3,000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed in TBST and incubated with the appropriate HRP-tagged secondary antibody (Sigma-Aldrich) for 3 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged proteins were incubated with 30 μl pre-washed anti-FLAG M2 affinity gel (Sigma, #A2220) for 3 h at 4 °C and washed four times with IP wash buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: PDT-tagged proteins - anti-HA antibody (Sigma, cat# 11666606001, dilution 1:1000), mf-Lon - anti-Flag (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2023Quote: ... expressing C-terminally 3x Myc-tagged TTP or ZFP36L1 were seeded onto poly-lysine (Sigma-Aldrich, P4832) pretreated glass coverslips for 48 h ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Genetics 2024Quote: ... APOBEC3C tagged with the superFLAG epitope was detected by immunoblotting with an anti-FLAG mouse antibody (Sigma anti-FLAG M2 antibody F3165 ...
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bioRxiv - Cell Biology 2020Quote: ... and human chorionic gonadotropin (Sigma), 48 hours apart ...
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bioRxiv - Biochemistry 2020Quote: ... human cathepsin S (Millipore Sigma), bovine spleen cathepsin C (Millipore Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... 20nM human insulin (Sigma-Aldrich), 2 μM rosiglitazone (Cayman) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% human serum (Sigma-Aldrich), 1x MEM Vitamins (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% human serum (Sigma-Aldrich) and 100 μg/ml primocin (Invivogen) ...
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bioRxiv - Cell Biology 2022Quote: ... 5% human serum AB (Sigma), penicillin-streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... Human Nuclei (1:300, Millipore), Human GFAP (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... Human CTSD ELISA (Millipore #1317) was performed on the culture media according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... and human IFN-γ (Millipore) were used for stimulation of cells ...
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bioRxiv - Bioengineering 2020Quote: ... or human plasma FN (Sigma) solutions ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... and human Fn (Sigma-Aldrich) as described previously (Bao et al. ...
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bioRxiv - Immunology 2021Quote: ... A human IgG (Sigma #I2511) or IgM (Sigma #I8260-1MG ...
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bioRxiv - Bioengineering 2021Quote: ... Human serum albumin (Sigma, USA) was used as a control ...
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bioRxiv - Bioengineering 2021Quote: ... human insulin (Sigma-Aldrich, I2643), single strand DNA (Sigma-Aldrich ...