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Citations for Millipore Sigma :
801 - 850 of 976 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was discarded and sample was resuspended in room temperature (RT) Liver Digest Buffer [0.15 mg/mL collagenase type XI (Sigma Aldrich; C9407), 0.3 U/mL dispase (Corning ...
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bioRxiv - Developmental Biology 2024Quote: ... They were blocked in 1% BSA in PBT for 1hr at RT and incubated overnight at 4°C with the monoclonal mouse anti-FLAG M2 antibody (Sigma, F1804) diluted 1:500 in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2024Quote: Retinal cryosections were rehydrated in 1X phosphate buffered saline (1X PBS) for 20 minutes at room temperature (RT) followed by incubation in 1.75 µg/mL Nile Red (Sigma, Cat# N3013) in 100% acetone for 1-hour at RT in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was stained with DAPI (1 mg/ml in water) for 5 min at RT and coverslips were mounted in fluoroshield (Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: Tissues were then washed with wash buffer and blocked for 11 hours at RT with 1X TBS with 5% (v/v) normal donkey serum (Sigma-Aldrich). The first antibody cocktail was prepared in 1X TBS-T 5% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... iPSC-CMs were cultured on Matrigel-coated coverslips and fixed at day 60 of differentiation in 4% Roti-Histofix (Carl Roth) at RT for 10 min and blocked with 1% Bovine Serum Albumin (BSA; Sigma-Aldrich) in PBS (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... We added 3 μl instead of 4 μl of master mix containing only 1.7 μl instead of 2.7 μl of 1 M Trehalose (Sigma-Aldrich) directly to the 2 μl cell lysate without a SPRI bead cleanup step ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were spun at 10,000 x g for 10 minutes and bacterial pellets were lysed by addition of BugBuster Master Mix (Merck Millipore) for 25 minutes at room temperature with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... spun at 10,000 x g for 10 minutes and bacterial pellets lysed by addition of BugBuster Master Mix (Merck Millipore) for 25 minutes at room temperature with gentle agitation ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated at a concentration of 2.5x105 per well in 12-well plates along with increasing volumes of master pool viral supernatant (0, 25, 100, 200, 500, 1000, and 2000µL) and polybrene (10µg/mL, EMD Millipore). Cells were then centrifuged at 1,500rpm for 2 hours at 37°C and incubated at 37°C overnight ...
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bioRxiv - Microbiology 2019Quote: ... a pellet corresponding to a 250 ml culture was resuspended in 1.5 ml of BugBuster Master Mix (MD Millipore) supplemented with 50 μl of DNAse I (5mg/ml) ...
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bioRxiv - Biophysics 2019Quote: ... The SU-8 master was silanized using vapor deposition of Trichloro(1H,1H,2H,2H-perfluoro-octyl) silane (Sigma) under vacuum ...
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bioRxiv - Microbiology 2023Quote: ... The cell suspensions were centrifuged at 10,000 x g for 10 minutes and bacterial pellets were lysed by addition of BugBuster Master Mix (Merck Millipore) for 25 minutes at room temperature with gentle agitation ...
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bioRxiv - Microbiology 2023Quote: ... 10 ng per well in triplicate was used with the FastStart Universal SYBR Green Master [Rox] (Sigma-Aldrich, USA). Primer pairs targeting 5’ UTRs of the selected genes were designed to amplify fragments less than 300 bp (Table S5) ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA corresponding to the fxn gene was amplified using KOD hot start master mix (Cat. N° 71842 Millipore) and FwDdFXNOE and RevDdFXNOE as primers (Table 1) ...
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bioRxiv - Pathology 2023Quote: ... cDNA was measured by quantitative PCR with FastStart Universal SYBR Green Master (Rox) (04913850001, Sigma-Aldrich, St. Louis, MO) using QuantStudio 6 (ThermoFisher) ...
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bioRxiv - Plant Biology 2023Quote: ... The negative masters were treated with a solution of 3-aminopropyltriethoxysilane (015-28251, Sigma-Aldrich, St. Louis, MO, USA) overnight under ambient conditions once before use ...
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bioRxiv - Biochemistry 2020Quote: ... Twenty μL of protein-compound mix was aliquoted per qPCR tube (Labtidebiotech, P01-0803E) followed by the addition of 5 μL of 1:400 diluted SYPRO Orange (Sigma, S5692). After incubation on ice for 20 minutes ...
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bioRxiv - Immunology 2019Quote: ... They were further characterized for expression of pluripotency genes using qPCR (REX1, SOX2, NANOG and HTERT) and immunofluorescence (TRA-1-60 (Millipore; MAB4360), SSEA-3 (Millipore ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from DUSP16 and KSR1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3 primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from IFIH1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3; primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of the diluted DNA was used as template in a qPCR reaction with SYBR Green JumpStart Taq ReadyMix (Sigma-Aldrich). Primer sequences targeting Cryptosporidium GAPDH were as follows ...
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bioRxiv - Cell Biology 2020Quote: ... Veeco Probes) with a spring constant of 60 pN/nm were functionalized by N1-(3-trimethoxysilylpropyl)diethylenetriamine (30 min, RT, Sigma Aldrich, 06666) followed by heating (30 min ...
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bioRxiv - Cell Biology 2020Quote: ... BSA was coupled to the PEG (3000) for 1h at RT with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 100 mM, Sigma Aldrich, 39391) and N-hydroxy-succinimide (NHS ...
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bioRxiv - Cell Biology 2019Quote: ... were treated for 20 min at room temperature (RT) with 200 µl of Bind-silane solution—a mixture of 714 µl 3-(Trimethoxysilyl)propyl methacrylate (3-TMP, Sigma-Aldrich, M6514), 714 µl of acetic acid in 10 ml of 96% ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... cells were treated with 4N HCl at RT for 30 min and incubated with mouse anti-BrdU at 1:100 (B2531, Sigma, Milan, Italy) in blocking solution (0.1% BSA in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... washed once with 5 mL ice-cold NETN250 buffer and lysed for 15 min at room temperature (RT) in ice-cold NETN250 buffer supplemented 5 mM MgCl2 and 125 U/mL benzonase (Novagen 71206-3). After addition of EDTA and EGTA to respective final concentrations of 5 mM and 2 mM to inactivate the benzonase and centrifugation for 30 min at 16,100 × g ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 1 hour at RT with the following antibodies diluted in blocking buffer: fibronectin (Sigma-Aldrich, St. Louis, MO) and α-smooth muscle actin (α-SMA ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Cell Biology 2021Quote: ... RPE organoids were fixed in 4 % paraformaldehyde (AppliChem GmbH, Darmstadt, Germany) for 20 min at room temperature (RT) and subsequently equilibrated to 30 % sucrose (Sigma-Aldrich, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 1 h incubation at RT in secondary antibodies (Anti-Rabbit: A0545; Anti-Mouse: A6782, Sigma-Aldrich Quimica S.A., Madrid, Spain) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h at RT followed by nuclear counterstaining with DAPI (1 μg/ml in 1 x PBS; Sigma-Aldrich, Germany) for 10 min at RT ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... and 0.4% Triton X-100 in Tris-bufered saline (TBS) at room temperature (RT) for 1 h and then incubated with primary antibodies against Sftpc (AB3786, Millipore; 1:500), Pdpn (8.1.1 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times with PBS and incubated 2 h at RT in the dark with FITC-coupled α-rabbit antibodies (1:200 in BSA-PBS, Sigma Aldrich). After washing and drying ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidified chamber at RT for 1 hr with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidity chamber at RT for 1 hour with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were rinsed once with PBS and fixed for 15 min at RT with 4% PFA (Sigma−Aldrich, cat. no. 158127) in 0.1 M phosphate buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... were incubated for 1 h at RT and after washing coverslips were incubated for 4 min with DAPI (1:4000, Sigma-Aldrich #D8417) and mounted with ProLong Gold Antifade reagent (Invitrogen #P10144).
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bioRxiv - Neuroscience 2020Quote: ... After rinsing three times for 10 minutes in TBS at RT sections were incubated with 4?,6-Diamidin-2-phenylindol (Dapi, Sigma, Cat# D9542) for 10 minutes at RT and washed once for 10 minutes in TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were rinsed three times with PBS and incubated 1 hour at RT with secondary antibodies and DAPI (1/10000, Sigma-Aldrich D9564) diluted in the same solution and rinsed 3 times with PBS before mounting on slides with VectaShield® Vibrance mounting medium.
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bioRxiv - Biochemistry 2022Quote: ... and a mixture of 3,3′-diaminobenzidine and urea-hydrogen peroxide (SigmaFast DAB tablets, Sigma-Aldrich, St Louis, MO, 30-60 sec, RT). The peroxidase/DAB reaction was stopped by rinsing the sections with 0.05M Tris-HCl buffer ...
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bioRxiv - Cell Biology 2022Quote: ... shaking at 60 °C) and alkylated (addition of 12 µL x 500 mM iodoacetamide, Sigma, in AB; 30 min. shaking at RT). Next ...
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bioRxiv - Neuroscience 2022Quote: ... Washed cells were first incubated for 1 h at room temperature (RT) with blocking solution (3% bovine serum albumin [BSA, Sigma, A7906] and 0.5% Triton X-100 [Sigma, X100] in 1xPBS) and then with primary antibodies for 2-3 h at RT ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... slides were dried in dark conditions at RT and a glass coverslip was mounted on top of each slide using Fluoromount mounting medium (Sigma Aldrich, USA). Z-stack images with a step size of ∼ 2 μm were acquired with an UPlanSApo 20× or a PlanApoN 60x objective using an inverted confo-cal microscope (FV1200 ...
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bioRxiv - Cancer Biology 2023Quote: ... HUVECs on gelatin-coated plates were incubated with gcEVs labeled with the fluorescent dye PKH-26 (Sigma-Aldrich, 10 min at RT). After washing ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... 500 μL of each lysate sample was incubated for 1 h at RT with 10 μL of 10 mM biotin picolyl azide (in DMSO) (Sigma Aldrich 900912), 10 μL of 50 mM TCEP (in H2O) ...
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bioRxiv - Plant Biology 2023Quote: ... A 90-min incubation at RT on a tube rotator was followed by supplementation with 10 mg/mL collagenase type II (Sigma-Aldrich, USA) in Ca2-free OR2 and ...
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bioRxiv - Cell Biology 2023Quote: ... Fixation was followed by blocking (1h, RT) with 3% bovine serum albumin (BSA/0.01% Tween™20/PBS) (Sigma, Cat#A-7906). Samples were incubated with primary antibodies (overnight ...