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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Mouse anti-FLAG M1 antibody and mouse anti-Sulfotyrosine antibody (Sigma) were used to detect the purified wild type C5aR and C5aRΔTyr with Y11F and F14F mutations respectively in the western blotting assays.
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-β-actin (Sigma, mouse monoclonal antibody, 1:5000). After incubation with HRP-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal mouse anti-mouse GFAP (1:2000; Sigma G-A-5), and monoclonal rat anti-mouse IL-6 (1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse anti-β-actin (Sigma, mouse monoclonal antibody, 1:5000). After incubation with HRP-conjugated secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Mouse Monoclonal anti-mouse Glutamin Synthetase (GS-6, 1:250, Millipore), Rabbit Polyclonal anti-SDS (PA5-58704) ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-NeuN (mouse monoclonal, 1:400, Millipore; catalog #MAB377, RRID:AB_2298772). This step was followed by incubation with secondary antibodies for 2h at RT to visualize primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... in a 10-cm dish transfected one day post seeding with packaging plasmids (1 μg VSV.G, 2 μg psPAX2 and 4 μg of transfer plasmid using PEI (Sigma-Aldrich). Virus containing supernatant was collected 72 hours post transfection ...
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Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: ... The plasmid mixture with the total concentration of 5mg/ml plasmid (equal molar mixture if there are multiple plasmids) containing 1mg/ml Fast green (Sigma) was injected into the embryonic lateral ventricle through a glass pipette ...
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bioRxiv - Bioengineering 2021Quote: ... DNA oligonucleotides and the GenElute HP Plasmid Miniprep and Midiprep kit used for plasmid purifications were acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase ...
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bioRxiv - Biophysics 2021Quote: ... Cells were harvested by centrifugation at 6000xg for 10min and the pellet was re-suspended in LB/kanamycin for plasmid extraction and purification using a GenElute plasmid miniprep kit (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... we used a dual-luciferase reporting system based on a plasmid containing the sequence for Renilla luciferase and the multiple cloning site (MCS) from the plasmid pMLuc-1 (Novagen) upstream of the firefly luciferase sequence in the plasmid pISO (addgene) ...
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bioRxiv - Plant Biology 2022Quote: ... coli cells that contained pL4440-CP were grown in LB supplemented with carbenicillin (100 μg/mL) followed by plasmid DNA extraction with the High Pure Plasmid Isolation Kit (Sigma). For the in vitro synthesis ...
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bioRxiv - Molecular Biology 2023Quote: ... and was mixed with 1 µg total DNA (100 ng receptor plasmid + 200 ng mGs plasmid + 700 ng salmon sperm DNA (Sigma) solubilized in 100 µL OptiMEM ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Genomics 2024Quote: ... and plasmid DNA was purified from the resulting cell pellet using a GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich #NA0310) following the manufacturer’s recommended protocol with elution in 3 mL H2O ...
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bioRxiv - Plant Biology 2020Quote: ... using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich). Inserts were sequenced (GATC Services ...
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bioRxiv - Biophysics 2021Quote: ... coli expression plasmid vector pET-28b (Novagen, MA) was used to construct all the gp17 WT and mutant clones (37) ...
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bioRxiv - Biochemistry 2019Quote: ... of the plasmid and BacVector3000 baculovirus DNA (Novagen) using the calcium phosphate co-precipitation method ...
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bioRxiv - Microbiology 2019Quote: Expression plasmids were transformed into BL21(DE3) (Novagen) and selected overnight at 37°C on Luria-Bertani (LB ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid-pET28a was purchased from Novagen (Germany). Ni-NTA agarose was purchased from G-Biosciences (USA) ...
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bioRxiv - Developmental Biology 2019Quote: ... Antigens were made using a pET28b (+) plasmid (Novagen) for expression of 6XHis tagged proteins ...
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bioRxiv - Biochemistry 2019Quote: ... all expression plasmids were created in pET21a (Novagen; N-terminal T7•tag® and C-terminal His•tag® ...
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bioRxiv - Biochemistry 2021Quote: ... in conjunction with a pET-21A plasmid (Novagen) containing WT Cre (provided by Gregory Van Duyne ...
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bioRxiv - Biophysics 2021Quote: ... Plasmids were transformed into Rosetta(DE3) pLysS (Novagen) E ...
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bioRxiv - Microbiology 2022Quote: ... and inserted into the expression plasmid pET30a (Novagen). Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3 ...
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bioRxiv - Neuroscience 2023Quote: ... gRNA expressing plasmids were purchased from Sigma Aldrich (U6-gRNA:hPGK-puro-2A-tBFP ...
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bioRxiv - Immunology 2023Quote: ... Expression plasmids based on the pET28a backbone (Novagen) were constructed by Gibson Assembly75 ...
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bioRxiv - Biochemistry 2024Quote: ... and then cloned into a pET28 plasmid (Novagen) with T4 DNA ligase (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: Recombinant plasmids were transformed into Tuner cells (Novagen) in Luria-Bertani (LB ...
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bioRxiv - Biochemistry 2024Quote: ... into the pET-28a expression plasmid (EMD Millipore). Protein was expressed and purified as described above ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with anti-mouse NeuN or anti-mouse parvalbumin (Millipore, MAB377 ...
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bioRxiv - Neuroscience 2020Quote: ... and 3) mouse anti-NeuN (mouse monoclonal, Millipore, cat # MAB377, 1:100); and mouse anti-S100β (Sigma S2532 ...
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bioRxiv - Microbiology 2021Quote: ... a mouse anti-FLAG primary (Sigma Mouse Anti-FLAG M2 monoclonal antibody) was used ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-mouse β-tubulin III (1:400, Millipore Sigma, catalog T2200), mouse humanized anti-GM2 (1:400 ...
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bioRxiv - Immunology 2022Quote: ... ng mouse IgG was generated using purified mouse IgG (Sigma Cat #15381); absorbance values from this standard curve were used to convert sample absorbance signals into mass equivalents for both anti-S and anti-N antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-pERK1/2 (T185/Y187) and mouse anti-Flag M2 (Sigma); and mouse anti-H3 and rabbit anti-H3K4 trimethyl (Active Motif).
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bioRxiv - Cancer Biology 2022Quote: ... For metastases delimitation cytokeratin (mouse anti-mouse cytokeratin pan-mixture, ascites, Sigma) and luciferase (rabbit anti-firefly luciferase ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was recovered from 150 ml of this overnight culture using the GenElute™ HP Plasmid Midiprep Kit (Sigma-Aldrich) as per the manufacturer’s instructions except for a modified elution step ...
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bioRxiv - Immunology 2022Quote: ... 150 ng of either pSPAX2 (for lentiviral plasmids) or HIT60 (for retroviral plasmids) and 150 ng pVSVg were diluted in 20 μl IMDM medium (Sigma-Aldrich) and mixed with 20 μl IMDM medium containing 1.2 μl of 1 mg/mL polyethylenimine (PEI ...
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bioRxiv - Microbiology 2019Quote: ... These clones were grown separately overnight in lysogeny broth (LB) medium and used for plasmid extraction with GenElute Plasmid Miniprep Kit (Sigma-Aldrich). PCR amplifications ...
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bioRxiv - Neuroscience 2022Quote: ... whole plasmids (Entry plasmids containing cmk-1 coding DNA sequences) were amplified with the KOD Hot Start DNA Polymerase (Novagen, Merck). Primers were phosphorylated in 5’ and were designed to contain the desired point mutation(s ...
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bioRxiv - Cancer Biology 2023Quote: ... the Delta-VPR packaging plasmid, and transfer plasmid (either pLJC2, pLentiCRISPR-v1, or pLentiCRISPRv2-Opti backbone) using X-tremeGENE 9 Transfection Reagent (Sigma-Aldrich). The medium was exchanged with fresh DMEM+2S 16 hr after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-HA (Sigma) was used at 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... αFlag (mouse, F1804, Sigma), αFlag-HRP (mouse ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-HA (Sigma, H3663 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse-anti-FLAG (Sigma), rabbit-anti-HA (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... raised in mouse (Millipore), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... anti-mouse IgG (Sigma) for VHA-B and chemiluminescent substrate (Peqlab) ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-MLH1 (Millipore). Images were collected using a DeltaVision system (Applied Precision ...
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bioRxiv - Cancer Biology 2020Quote: ... RalB (mouse, 04037; Millipore). The following secondary antibodies were used ...