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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for Bcl 2 Binding Component 3 BBC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... coupled with anti-IL-2 Rα antibody (Sigma, clone 7G7/B6).
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 2 µg of antibody (anti-V5, Millipore, AB3792) in PBS for 90 min at 4° C ...
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bioRxiv - Cell Biology 2021Quote: ... 4°C) and the cell pellets washed twice with binding buffer (100μl/well) and resuspend in PI solution (Sigma; 50μg/ml in binding buffer; 100μl/well) and stored at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... samples were incubated with 2 % (v/v) primary anti-tubulin antibody (clone B-5-1-2, Sigma-Aldrich) in blocking buffer for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... in Biotin binding buffer (5 mM Tris-HCl pH 7.5 [Sigma] ...
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bioRxiv - Microbiology 2019Quote: High-binding 96-well plates (Cat# CLS3361; Millipore Sigma, Burlington, MA) were either coated directly with 500 ng/well of recombinant DENV2 16681 E protein (Cat# DENV2-ENV-500 ...
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bioRxiv - Immunology 2022Quote: ... Binding intensity was revealed by addition of TMB reagent (Sigma, France) for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Digested glycans were cleaned using a PVDF protein-binding membrane (Millipore) and analyzed as described above.
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bioRxiv - Molecular Biology 2019Quote: ... six repeats of CASTing selected binding sites synthesized by SIGMA-ALDRICH were introduced upstream of the TKmini promoter ...
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bioRxiv - Synthetic Biology 2019Quote: ... ribosomal binding sites and terminators were ordered as oligonucleotides (Sigma-Aldrich) or were obtained from previous studies (32 ...
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bioRxiv - Plant Biology 2021Quote: ... One mL of lysate binding buffer (1 M LiCl Sigma-Aldrich, Cat ...
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bioRxiv - Immunology 2022Quote: ... Binding intensity was revealed by addition of TMB reagent (Sigma, France) for 6 min ...
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bioRxiv - Cell Biology 2022Quote: For binding assays with recombinant KAP (Sigma Aldrich, Cat. No. SRP5175) or CAK (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... Digested glycans were cleaned using a PVDF protein-binding membrane (Millipore) and analyzed as described above.
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bioRxiv - Genetics 2019Quote: ... filtered by using a 0.45 μm low protein binding membrane (Millipore), and then centrifuged at 30,000 rpm and 4°C for 2.5 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... and nonspecific binding was blocked with 10% normal donkey serum (Sigma) for 4 hours at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant rabbit anti-pan-ADP-ribose binding reagent MABE1016 (Merck Millipore) was used 1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Unspecific binding was blocked with 10% goat serum (Millipore, Cat. #S26) and cultures were incubated overnight at 4°C with primary antibodies diluted in TBS/10% goat serum ...
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bioRxiv - Bioengineering 2022Quote: ... anti-Fatty acid binding protein 1 (FABP1) (1:50, Sigma-Aldrich) and anti-cytokeratin 20 (CK20 ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a 0.45-µm low-protein-binding Durapore filter (Millipore), and stored at −80°C until use ...
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bioRxiv - Immunology 2023Quote: ... HEL-binding cells were stained with 50 ng/mL HEL (Sigma) or HEL3x (R ...
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bioRxiv - Plant Biology 2023Quote: ... The membranes were probed with anti-GAL4 DNA Binding domain (Sigma) antibody for the OsExo70F3 protein in pGBKT7 and with the anti-GAL4 activation domain (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... Surface binding was detected by development with BM-Purple (Sigma 11442074001). Images were obtained with DMIL microscope/Canon DS126311 camera (LEICA ...
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bioRxiv - Biochemistry 2023Quote: ... filtered through a 0.45 mM low protein-binding PVDF filter (Millipore) and applied to a 1 mL StrepTrap HP column pre-equilibrated in Strep-start buffer (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... for the fibronectin-binding assay and 10 μg of fibrinogen (Sigma) for the fibrinogen-binding assay in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... and slices were incubated overnight at 4 °C in a PBST-based antibody carrier solution containing 3% normal goat serum and rabbit anti-TPH2 primary antibodies (Millipore, ABN60) diluted 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by primary antibody incubation in 10% sheep serum at 4°C for 3 days (Anti-SCN5A antibody produced in rabbit (Sigma SAB2107930) 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Unspecific antibody binding was prevented by incubating the blots in blocking solution containing 5% non-fat milk or 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in 1x Tris-buffered saline with 0.1 % Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Bioengineering 2021Quote: ... 39.5 wt% TEGDMA and 1 wt% camphorquinone(the three basic and critical hydrophobic components for all the dental adhesives, all purchased from Sigma) was produced in our lab according to an open formula of Heliobond (an enamel adhesive ...
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bioRxiv - Cell Biology 2022Quote: ... and consists of the following components mixed together shortly before use: 10 volumes of 0.25 M zinc sulfate (Sigma-Aldrich), 2 volumes of 36.5-38% (w/v ...
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bioRxiv - Synthetic Biology 2019Quote: ... To test the effects of mucin addition43 and iron depletion,44 overnight-grown cultures were inoculated in a 1:200 dilution in LB supplemented with different components [0.4% (w/v) of bovine submaxillary gland mucin (Sigma-Aldrich), or 200 µM of 2,2’-dipyridyl (Alfa Aesar) ...
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The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... An aliquot of homogenate was used for bicinchonic-acid protein-level determination using commercial components (Sigma, #B9643, #C2284, and #P0914). Glycogen content was assayed by hydrolyzing glycogen into glucose with amyloglucosidase (Sigma ...
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bioRxiv - Biophysics 2020Quote: Enzymatic degradation was targeted to the two main components of cartilage ECM: collagen network with collagenase D (0.29 U/mg, Sigma Aldrich) and proteoglycans with trypsin (T 4174 ...
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bioRxiv - Microbiology 2022Quote: ... the plates were washed 3 times with 1x PBS to remove the supernatant and the degradation of the pre-coated ECM components was detected using mouse anti-fibronectin (F7387, 1:5,000; Millipore Sigma), and rabbit anti-laminin (L9393 ...
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bioRxiv - Zoology 2023Quote: ... Fatty acids were identified by comparing the known fatty acids standards (Supelco 37 Component FAME Mix, Sigma-Aldrich, Darmstadt, Germany).
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bioRxiv - Synthetic Biology 2024Quote: ... RNPs were produced by complexing a two-component gRNA (Edit-R, Dharmacon Horizon) to Cas9 protein with the addition of a PGA (Sigma) electroporation enhancer ...
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bioRxiv - Developmental Biology 2023Quote: ... at E21.5 rat fetuses were placed under isoflurane anesthesia and intracardiac perfusion was performed with PBS to wash out blood components followed by 1mg/ml 10,000 Da FITC (Sigma Aldrich) in PBS (pH 7.0 ...
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bioRxiv - Pathology 2020Quote: Ela-Cre AT-1fl/+ and Ela-Cre AT-1fl/fl mice at 2-3 months of age were treated with tamoxifen (MP Biomedicals, 3mg/40g BW dissolved in 98% corn oil [Sigma] and 2% ethanol [Sigma]) by oral gavage once daily for three days to induce Cre recombinase activity ...
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bioRxiv - Microbiology 2019Quote: ... A 200 μL aliquot of a solution containing 100 μg/μL of XTT (2, 3-(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) (Sigma-Aldrich, St Louis, MO, USA) and 10 μg/μL of PMS (phenazine methosulfate ...
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bioRxiv - Cancer Biology 2020Quote: ... Moncolonal pan-RAS antibody (clone Ab-3; OP40-100UG; 1:1000) was obtained from Millipore. Rabbit polyclonal antibodies against SHP2 (sc-280 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed twice with PBS and anti-Gal-3 antibody (1:50; Sigma-Aldrich) was added ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma, #A2220). For the IP ...
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bioRxiv - Molecular Biology 2019Quote: ... or anti-Strep mouse IgG monoclonal primary antibody (Sigma, 71590-3; 1:1000-1:2000) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were diluted in DMEM high 3 glucose media containing 0.7% Low IgG BSA (Sigma), 1X Sodium Pyruvate ...
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bioRxiv - Microbiology 2020Quote: ... and probed with appropriate antibodies in PBS with 3% milk and 0.1% Tween20 (Sigma-Aldrich). Rabbit and mouse anti-SFP1 serum were used at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were probed using antibodies for ATG16L1 (MBL M150-3) and ACTB/actin (Sigma, A5441). Primary antibodies were detected using IRDye labelled secondary antibodies (LI-COR biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Coverslips were blocked with 3% BSA and incubated with primary antibodies (RPA - ab79398, H2AX - Millipore-05-636 ...
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bioRxiv - Microbiology 2024Quote: ... and co-IPs carried out using 3 μg monoclonal anti-FLAG M2 antibody (Sigma-Aldrich) or 30 μg polyclonal FlgV antibody or 30 μg polyclonal KhpB antibody and 20 μl of Pierce protein A/G magnetic beds ...
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bioRxiv - Physiology 2024Quote: ... Primary antibodies were used at described dilutions in 3% bovine serum albumin (BSA; Sigma-Aldrich) in Tris-buffered saline with Tween 20 and incubated overnight (4°C) ...