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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 4-Chloro-2-Methylthiopyrimidine (Sigma, 145289), GW3965 (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×105 KP cells (n=3) were plated in 6-well plates overnight in RPMI medium (Sigma). After 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: 2×105 KP cells (n=3) were plated in 6-well plates overnight in RPMI medium (Sigma). After 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×105 KP cells (n=3) were plated in 6-well plates overnight in RPMI medium (Sigma). After 24 hours ...
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bioRxiv - Bioengineering 2020Quote: ... nalidixic acid (NA; N8878, BCBW6556), and 4′,6-diamidino-2-phenylindole (DAPI; D9542, 28718-90-3) were purchased from Sigma Aldrich, USA ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... 2 mM Ca(NO3)2 ×24H2O (13477-34-4, Sigma), 2.5 mM KH2PO4 (7778-77-0 ...
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bioRxiv - Neuroscience 2021Quote: Retinal lesions were induced by a single intravitreal injection of 0.5 - 1 nL of freshly prepared 100 mM N-Methyl-D-aspartic acid (NMDA, M3262, Sigma). Briefly ...
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bioRxiv - Physiology 2024Quote: ... Some vessel segments were incubated with L-NAME (N-nitro-L-arginine methyl ester 100 μmol.L-1, Sigma-Aldrich), The mechanical activity was isometrically recorded using a force transducer and a data acquisition system (DATAQ Instruments ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then treated for 15 min up to 4 h at 37°C with 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, #M5655-1G) in 5 mM PBS ...
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bioRxiv - Bioengineering 2019Quote: ... any excess solution was carefully removed with a Kimwipe before being frozen in 2-Methyl Butane (Sigma-Aldrich). Each experiment was repeated five times ...
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bioRxiv - Immunology 2019Quote: ... MEK1/2 inhibitor MEKi-A (AZD6244) was formulated in sterile vehicle with 0.5% (Hydroxypropyl)methyl cellulose (Sigma-Aldrich) and 0.1% polysorbate 80 (v/v ...
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bioRxiv - Immunology 2022Quote: ... and 95% - for at least 2 hours each before incubation with a methyl salicylate solution (Sigma-Aldrich, M6752) for 30-60 minutes in a custom metal chamber with 0.2mm coverslip glass bottom ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were removed and rapidly frozen by immersing in 2-methyl butane (Cat. M32631, Sigma-Aldrich, Darmstadt, Germany) previously chilled in dry ice ...
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bioRxiv - Biophysics 2022Quote: Methyl-β-cyclodextrin-treated cells were incubated in 10 mM methyl-β-cyclodextrin (Sigma-Aldrich, C4555-5G) for 15 min before imaging ...
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bioRxiv - Bioengineering 2023Quote: ... methyl-β-cyclodextrin (MβCD; Sigma-Aldrich, cat# C4555, 1 mM) was added to the medium of some groups for 1 h at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... the nuclei were labeled with a 1:10,000 dilution of DAPI (4’,6-Diamidino-2-phenylindole; Sigma, D9542) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... DNA staining was achieved by addition of 1 μg/ml 4',6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Coverslips were mounted using mounting medium (20 mM Tris pH8 ...
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bioRxiv - Plant Biology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich catalogue no D9542, 1:600 dilution). Figure 4 shows images of seedlings from 1 of 6 experiments ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were stained by addition of 1 µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) containing FluoromountG (Southern Biotech) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:10,000 dilution with milliQ water, Sigma-Aldrich) for 10 minutes ...
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bioRxiv - Physiology 2020Quote: ... Nuclei were counter-stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 1/5000) (Sigma-Aldrich, Dorset, UK). Imaging was performed on a Leica TCS SP5 confocal microscope (Leica Microsystems ...
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bioRxiv - Immunology 2020Quote: Cell suspensions were stained for viability with either 1:3000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) or 1:1000 Zombie Aqua (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... with 1:30,000 of the fluorescent marker of nucleic acids 4’,6-diamino-2-fenilindol (DAPI; Sigma-Aldrich). We took photographs of the cells using an epifluorescence microscope (DMIL LED ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) 1:1,000 (Sigma-Aldrich Sweden AB, Stockholm, Sweden). Invasion of the cells was measured by counting the nuclei of the cells that migrated through the filter pores towards the chemoattractant (6% FBS) ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated with DAPI (4’,6-Diamidino-2-phenylindole dihydrochlorid) solution(D9542, Sigma-Aldrich, diluted 1:10000) for 7 minutes at RT and subsequently mounted in ProLong Gold Antifade mounting medium (cat ...
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bioRxiv - Biophysics 2020Quote: ... (D9891), 2-aminoethoxydiphenyl borate (2-APB) (D9754) and poly(methyl methacrylate) (PMMA, Mw~15,000) (200336) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Thapsigargin (586005 ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 μl of 5mg/ml MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma) was added to each well ...
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bioRxiv - Microbiology 2019Quote: The mitochondria-dependent reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) to formazan was used to assess cell viability ...
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bioRxiv - Cancer Biology 2019Quote: Cell viability was accessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) assay ...
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bioRxiv - Cancer Biology 2019Quote: Cell viability was accessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) assay ...
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bioRxiv - Physiology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay from Sigma (St. Louis, MO), and D-luciferin from Gold Biotechnology (St ...
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bioRxiv - Biophysics 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were procured from Sigma Aldrich, St ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay (Sigma). The absorbance was measured using a Synergy™ 4 plate reader (Bioteck ...
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bioRxiv - Cell Biology 2022Quote: ... MTT reagent [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma-Aldrich, St. Louis, MO), dissolved in PBS ...
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bioRxiv - Pathology 2019Quote: ... a cell-permeant NO scavenger 2-(4-car-boxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) (Sigma) at a high concentration of 500 μM was included ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure D-2959) photoinitiator (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Systems Biology 2020Quote: BALB/cJ mice were subjected to cutaneous Oxazolone (Oxa) challenge by applying 5% 4-Ethoxymethylene-2-phenyl-2-oxazolin-5-one (Sigma-Aldrich) in acetone and olive oil topical to the skin as described [74] ...
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bioRxiv - Immunology 2022Quote: Mice were sensitized on the shaved back-skin for two consecutive days with 50 μl of 5% Oxa (4-Ethoxymethylen-2-phenyl-2-oxazolin-5-on, Sigma-Aldrich) diluted in methanol and acetone (1:1 ...
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bioRxiv - Microbiology 2019Quote: ... 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Sigma-Aldrich, USA), sodium hydroxide (Sinopharm Chemical Reagent Co. ...
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bioRxiv - Immunology 2022Quote: ... spleens were fixed for 2 h at 4°C in 2 mL of 4% paraformaldehyde (Millipore Sigma) followed by an overnight incubation at 4°C in 2 mL of 30% sucrose (Millipore Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 2 h or 4-hydroxytamoxifen (4-OHT, Sigma, 10 µM) for 4 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 µM of the nonspecific nitric oxide synthase inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME [Sigma Aldrich], n=5), or 1.0 mM P188 and 100 µM L-NAME (n=5) ...
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bioRxiv - Genomics 2021Quote: ... 10 mM MgCl2,10 mM Tris pH 7.4, 1 mM CaCl2) with increasing amounts (0, 0.5, 1, 2, 5, 7.5, 10 U) of MNase (Sigma) at 30°C for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... the samples were dissolved in 50 μL of 20 mg/ml methoxyamine hydrochloride in pyridine for 1.5 h at 33°C followed by derivatization with N-Methyl-N-(trymethylsolyl)trifluoroacetamide (MSTFA, Sigma Aldrich) for 2 h at 35°C.
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bioRxiv - Plant Biology 2021Quote: ... The sample was then derivatized by adding 60 μL N-methyl-N-(trimethylsilyl)trifluoro acetamide (MSTFA) (Cat# 69479; Sigma-Aldrich) and incubating for 30 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Cell Biology 2019Quote: ... and 2 mg/ml N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) (Sigma, 03450) in 50 mM HEPES were added on top of the hydrogels and incubated for 30 min at RT ...