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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for 3 Methyl 1 6 naphthyridine 2 carboximidamide hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... and 1 mM (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox; 238813-5G, Sigma-Aldrich). To obtain images for training the DeepSTORM model ...
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bioRxiv - Neuroscience 2021Quote: ... The nuclei were stained with DAPI (1:1000, 4’, 6-diamidino-2-phenylindole dihydrochloride; Sigma). The absence of labeling when using sense probes was used to confirm the specificity of the anti-sense probes ...
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bioRxiv - Systems Biology 2021Quote: ... diluted 1:200 in blocking reagent) and 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) was used at 200 ng/mL for nuclei staining ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were stained using 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich #D8417, 1:1000).
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bioRxiv - Immunology 2024Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, 1 µg/ml, Sigma, USA) for 10 min in the dark.
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were stained with DAPI (4′, 6-diamidino-2-phenylindole, 1 μg/mL, Sigma-Aldrich). Images were acquired on a point-scanning confocal microscope SP8 Leica with an oil 40x objective 1.25 N.A ...
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bioRxiv - Biophysics 2023Quote: ... Nucleus were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1:1000; Sigma-Aldrich) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... The sections were then stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:3000, Sigma) diluted in TBS for 1 min and then finally rinsed three more times with TBS ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1 μg/mL; Sigma-Aldrich) and coverslipped with Vectashield mounting media (Vector Labs ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were counterstained with 4′,6-diamidino-2-phénylindole (DAPI; 1 μg/mL; Sigma-Aldrich) and cover slipped with Vectashield mounting medium (Vector Labs ...
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bioRxiv - Neuroscience 2024Quote: ... Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma, #D9542) during 10 minutes in PBS1X ...
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bioRxiv - Developmental Biology 2020Quote: ... paws were incubated twice for 5min with methanol 50% BABB (1/3 benzylalcohol and 2/3 benzylbenzoate, from Sigma), and then three times in pure BABB for 5min each (or until the sample is cleared) ...
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bioRxiv - Molecular Biology 2021Quote: ... selected for 2-3 weeks with 1 µg/ml of puromycin (Sigma- Aldrich), and surviving cells were fixed with methanol and stained with Giemsa (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich) added fresh] or RIPA buffer [25 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... and (3) GluA2 (mouse anti-glutamate receptor 2; Millipore; used at 1:2000). Cochlear pieces were incubated in species appropriate secondary antibodies coupled to Alexa Fluors in the red ...
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bioRxiv - Cancer Biology 2021Quote: ... or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437, Sigma Aldrich) for 24 hours prior to collection of cells for RNA extraction.
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM PMSF and phosphatase inhibitor cocktail 2 and 3 from Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 to 3 million cells were cross-linked using 1% formaldehyde (Sigma, F8775) for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5mM Na3VO4 and 1% each of phosphatase inhibitor cocktails 2 and 3 (Sigma) and set on ice for 10 minutes prior to sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 3:2:1 in HEK 293T cells (Sigma Aldrich) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044). Lysates were cleared at 17,000g ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM PMSF and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich, Switzerland). The insoluble fraction was then sonicated using a fine probe 15 times at 0.5 sec pulse at an amplitude of 20% ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 mM EDTA) supplemented with phosphatase inhibitor cocktail 2 and 3 (Sigma), and protease inhibitor cocktail (cOmplete Protease Inhibitor ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The starting materials methyl 4-nitrobutyrate and methyl 4- oxobutanoate were obtained commercially (Sigma-Aldrich). Compounds CP-24 to CP-30 were formed by transesterification of dimethyl CP-23 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the leaves from five-week-old Col-0 plants were sprayed 6 times over 48 h with methyl jasmonate (MeJA) (Sigma-Aldrich, Cat # 392707 ...
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bioRxiv - Cell Biology 2024Quote: ... islets isolated from chow-fed C57BL/6 WT mice were pre-incubated in 20 mM cholesterol loaded onto methyl-β-cyclodextrin (MβCD) (C4951, Sigma Aldrich) diluted in 1X Krebs-Ringer bicarbonate-Hepes (KRBH ...
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bioRxiv - Neuroscience 2021Quote: ... animals received daily intraperitoneal (I.P) injections of L3,4-dihydroxyphenylalanine methyl (L-DOPA, 3 day at 3mg/kg, then 6mg/kg, Sigma-Aldrich) and the peripheral DOPA decarboxylase inhibitor bensezaride hydrochloride (12mg/kg ...
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bioRxiv - Microbiology 2023Quote: ... embryos were mechanically dechorionated and anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine, Sigma-Aldrich). Zebrafish embryos were individually infected by microinjection with 1 nl of P ...
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bioRxiv - Cell Biology 2024Quote: ... 3) paraquat: worms were placed on standard NGM/OP50 plates treated with 25 mM paraquat (methyl viologen; Sigma Aldrich) for 24 hours ...
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bioRxiv - Biochemistry 2020Quote: ... the acid insoluble fraction was incubated for 18 hours at 4°C with 500 μL of 6 M guanidine hydrochloride/100mM TRIS buffer (pH 7.4, Sigma-Aldrich, U.K.). The buffer was exchanged into 100 μL of 50 mM ammonium acetate (Scientific Laboratory Supplies ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was washed 3 times in a solution of 0.3 M guanidine hydrochloride (Sigma, Cat. No. SRE0066) in 95% ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... they were incubated with 4 mg/mL of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma-Aldrich, Schnelldorf, Germany) dissolved in 100 mM 2-(N-morpholino ...
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bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...
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bioRxiv - Bioengineering 2024Quote: ... the matrices were incubated with 4 mg/mL N-(3- dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma‒Aldrich, Schnelldorf, Germany) dissolved in 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Systems Biology 2023Quote: ... 6 M Urea and 1% Sodium Dodecyl Sulphate supplemented with 100mM phenylmethylsulfonyl fluoride and 1% phosphatase inhibitor cocktail 3 (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Developmental Biology 2021Quote: Larvae at 2-6 dpf were first anesthetized with 0.03 % Ethyl 3-aminobenzoate methane sulfonate salt (Sigma-Aldrich, St. Louis, MO, USA) and pinned onto a Sylgard-filled recording chamber (I-2450 ...
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bioRxiv - Plant Biology 2022Quote: ... DSF supplementation was performed by adding with 5 μM of cis-11-methyl-2-dodecenoic acid (Sigma) to the media.
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bioRxiv - Biophysics 2022Quote: ... We dissolve 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM) powder (Sigma Aldrich) in HEPES buffer (pH 7.18 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... airway mechanics were measured by challenging pigs to increasing concentrations of methacholine hydrochloride (MeCh) (0, 0.5, 1, 2, 4, 8, 16, and 32 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) via an Aeroneb™ nebulizer (Aerogen Ltd ...
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bioRxiv - Biochemistry 2021Quote: ... 250 μL Protease Inhibitor Cocktail (18 mM 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) (Sigma, No. A8456), 1.7 mM bestatin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was reduced with a final concentration of 10 mM Tris(2 - carboxyethyl)phosphine hydrochloride (TCEP) (Sigma) and alkylated with 40 mM 2-chloroacetamide (CAA ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µg of protein was reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma Aldrich) for 2 min and alkylated with 20 mM chloroacetamide (CAA ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: To measure symplasmic connectivity using the HPTS (8-Hydroxypyrene-1, 3, 6-trisulfonic acid trisodium salt, SIGMA-ALDRICH) dye movement assay ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich) and 5 ng/ml FGF2 (Peprotech) ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Cell Biology 2024Quote: ... two siRNA sequences targeting different regions of NOK were designed as #1: 5’-GCAAGAAACAUUCAUGCAU-3’ and #2: 5’-GUCUUUCCCAGGGACACAA-3’ (SASI_Hs01_00043410 and SASI_Hs02_00351937, Sigma, St. Louis, MO, USA). The control siRNA is a scrambled sequence (5’-UUCUCCGAACGUGUCACGUdTdT-3’ ...
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bioRxiv - Biophysics 2020Quote: ... Imaging was done in a solution of HM containing 1% Methyl Cellulose (Sigma) in order to increase the viscosity of the solution and allow more precise visualization of tissue and bead movement ...