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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Multiplex ELISA was performed on a Luminex FlexMAP 3D (Millipore Sigma) using a premixed 32-plex cytokine magnetic bead panel (Millipore Sigma ...
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bioRxiv - Physiology 2021Quote: ... 100 μl of 3,3’,5,5’-tetramethylbenzidine (TMB) Elisa substrate (T0440; Sigma) was added and the reaction stopped after 10 min using 2 M H2SO4 ...
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bioRxiv - Genetics 2020Quote: ... Adiponectin ELISA (mouse Adiponectin, EZMADP-60K, EMD Millipore Corporation, Darmsbalt, Germany) and Cholesterol Assay (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Leptin concentrations were determined by ELISA (Millipore, St. Charles, MI, USA). The blood glucose levels were measured using a Glucocard glucometer (Arkray ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Cytokines and CCL5 were measured by Multiplex ELISA (MCYTOMAG-70K, Millipore) or Enzyme Linked Immunosorbent Assay (ELISA ...
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bioRxiv - Immunology 2023Quote: ... All ELISA assays were developed using TMB liquid substrate system (Sigma), stopped with 2N sulfuric acid ...
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bioRxiv - Immunology 2022Quote: ... Components C through F are the same for all 5 kits (Sigma-Aldrich) and are as follows ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 µg total protein was denatured for 5 min at 70°C in 1X Laemmli Buffer (Sigma Cat # S3401-10VL), resolved on SDS-acrylamide gels and electroblotted to nitrocellulose ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies at appropriate dilutions (Supplementary Table 3) were prepared in blocking buffer with 0.1% Tween 20 (Sigma, 9005-64-5). The membrane was incubated in the primary antibody solution overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were first washed with 1x PBST (1x PBS with 20% Tween20) and then in blocking serum (5% serum (Cat. No. D9663, Sigma-Aldrich), 1% bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were blocked in PBS + 0.1% Tween 20 + 5% powdered milk for 1 h and then incubated with anti-HA (Sigma-Aldrich, H6908) or Anti-Actin monoclonal antibody made in mouse (ThermoFisher Scientific AM4302 ...
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bioRxiv - Physiology 2022Quote: ... 117 NaCl, 10 KCl, 1 MgCl2, 10 creatine, 20 taurine, 5 adenosine, 2 L-carnitine, 10 glucose, 10 HEPES [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then stained with 20 µl MTT 5 mg/ml stock solution (Thiazolyl Blue Tetrazolium Bromide: Sigma-Aldrich Cat: 5655) for 4 hours and then dissolved in DMSO ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
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bioRxiv - Microbiology 2022Quote: ... 5% lysed horse blood (defibrinated 50% stock, Hatunalab AB Cat. N° 139) and 20 mg/mL β-NAD (Sigma, Lot# SLCD5502). Prior to use the 50% horse blood stock was freeze thawed five times and clarified via centrifugation twice for 30 minutes at 18,000 rpm at 4 °C and then filtrated using 0.2 μm membrane filter ...
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bioRxiv - Microbiology 2020Quote: 13C-NMR samples were prepared containing 430 μl bacterial sample in a 5 mm NMR tube (Norell, Morganton, NC, USA) with 20 μl D2O (Sigma Aldrich) and 10 μl of 200 mM 13C-urea (chemical shift and concentration reference ...
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bioRxiv - Neuroscience 2019Quote: ... 25-40 animals were incubated for 3h at 20°C in 5% (w/v) of blue food dye (Erioglaucine disodium salt, Sigma-Aldrich) in E ...
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bioRxiv - Neuroscience 2019Quote: ... Animals were glued with Wormglu along the body to a 5% agarose pad on a glass slide and covered with M9 or 20 mM histamine (Sigma, H7125) and a coverslip.
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bioRxiv - Cancer Biology 2019Quote: ... After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA) in phosphate-buffered saline (PBS) with 0.5% Tween 20 (Sigma-Aldrich, Belgium), the membranes were incubated overnight at 4°C with the primary antibodies (Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... tubes were thawed on ice, and 75 μL of sample was combined with 5 uL of 20 mM internal standard (norleucine and norvaline, (Sigma-Aldrich) and U-13C6 glucose (Cambridge Isotope laboratories)) ...
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bioRxiv - Bioengineering 2021Quote: ... The primary antibody buffer was 3% horse serum and 5% DMSO dissolved in 1X PBS with 0.2% Tween-20 (Sigma Aldrich, P2287) and 10 μg/mL heparin (Sagent Pharmaceuticals ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS was incubated in each well for 5 minutes and cells were washed with 2x SSCT (2x SSC with 0.1% TWEEN® 20 (P9416-100ML; Sigma-Aldrich). For storage ...
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bioRxiv - Biochemistry 2021Quote: ... Input (5% of total) was collected and the remainder was mixed with 20 μL of anti-FLAG Sepharose 4B beads (Sigma Aldrich) at 4 °C for 2 h on a rotator ...
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bioRxiv - Bioengineering 2021Quote: ... once with a 1% SDS-TRIS pH 11 solution (5% v/v of 20% sodium dodecyl sulfate #05030, from Sigma Aldrich and 2.4 % w/v TRIS base PBP151-500 from Fisher Scientific in reverse osmosis water ...
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bioRxiv - Neuroscience 2020Quote: ... hyposmia was induced by bilateral intranasal injections with 20 μL of 5% (0.17M) zinc sulfate (Sigma-Aldrich, catalog #7446-19-7) in male C57BL/6N mice ...
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bioRxiv - Molecular Biology 2022Quote: ... The extracted proteins were analyzed using 5–20% gradient sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) electrophoresis and transferred onto Immobilon Transfer Membranes (Millipore, INYC00010) for immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... Printed discs were incubated in 0.1 mg/mL FITC-labelled dextran molecules (size either 3-5 kDa, 10 kDa, 20 kDa or 70 kDa (Sigma-Aldrich)) dissolved in PBS with 20mM CaCl2 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... for 5 days at 37°C in PTwH buffer (PBS; 0.5% Tween-20, Fisher, BP337; 10mg/L Heparin, Sigma-Aldrich, H3393). After 2 days of washing with PTwH ...
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bioRxiv - Neuroscience 2021Quote: ... 2 x 100% Methanol in double distilled H2O for 30 min each) followed by an overnight step in 5% H2O2 / 20% DMSO (276855, Sigma Aldrich) / Methanol at 4°C to reduce background fluorescence and increase permeability ...
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bioRxiv - Immunology 2022Quote: ... followed by blocking for 1 h with 5% nonfat milk in Tris-buffered saline-Tween 20 buffer and probing with antibodies against Strep Tag (SAB2702215, Sigma-Aldrich), γ-catenin (sc-514115 ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were washed with wash buffer (10% formamide in 1X SSC and 0.1% Tween-20) for 5 min, then incubated with the hybridization mix (10% formamide, 1X SSC, 10% Dextran sulfate (w/v) (Sigma, D8906), 100nM each in situ probe ...
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bioRxiv - Systems Biology 2019Quote: ... The extracted proteins were analyzed by 5-20% gradient SDS-PAGE and transferred onto Immobilon transfer membranes (Millipore, Billerica, MA, USA) for immunoblotting analyses ...
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bioRxiv - Molecular Biology 2020Quote: ChIP experiments were performed in AtT-20 cells as previously described (29) using 5 μg of antibody (anti-FLAG-M2, mouse monoclonal, Sigma F3165). ATACseq was performed as previously described (4) ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated for 3 h at 35°C in extracellular solution (bubbled with 20% O2, 5% CO2 and 75% N2) containing 7.5 μM propidium iodide (PI; Sigma-Aldrich, 81845) and isolectin B4 conjugated to Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Synthetic Biology 2020Quote: ... and started with a batch phase using 400 mL synthetic medium containing 20 g/L of glucose and 5 mL Antifoam 204 (Sigma A6426). The temperature was maintained at 30°C and the pH was kept constant at 5.0 by dropwise addition of a 10M KOH solution ...
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bioRxiv - Immunology 2020Quote: ... Nonspecific binding sites were blocked by incubation in 20% normal goat serum (NGS) and 5% bovine serum albumin/PBS (BSA, Fraction V; both Sigma-Aldrich). Cryosections were stained with anti TNF-α ...
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bioRxiv - Microbiology 2019Quote: ... and susceptibility to chlorhexidine was tested with discs (Hardy) impregnated with 5 μl of a 20% chlorhexidine gluconate solution (Sigma-Aldrich). Species confirmation was performed with MALDI-TOF (Bruker Biotyper ...
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bioRxiv - Plant Biology 2020Quote: ... and added to the growth medium to obtain different final concentrations (0.1, 1, 5, 10, 20, 50 µM. Lincomycin (Sigma-Aldrich L6004) was added directly to the growth medium to obtain 0.5 mM as described before (Martín et al. ...
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bioRxiv - Genomics 2019Quote: ... The dishes were then transferred to wet ice and 400 ul of lysis buffer (1X polysome buffer (20 mM Tris-Cl pH 7.4, 150 mM NaCl, 5 mM MgCl2, with 1 mM DTT (Sigma Aldrich #43816) and 100 ug/ml cycloheximide added freshly ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were blocked in %5 BSA for 2 h at room temperature and incubated with anti-desmin antibody (1:20; Sigma, D8281) for two overnights ...
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bioRxiv - Biochemistry 2022Quote: ... for 24 hours and serum starved with OptiMEM for 48 hours prior to fixation with 3% PFA/PBS for 3 minutes in room temperature followed by a 5 min incubation at −20 °C with pre-cooled methanol (Sigma Aldrich). Fixed cells were blocked with 3% BSA (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... the column was filled by syringe with 5 mL of thrombin solution (20 U/mL in PBS, pH 7.3) (Sigma-Aldrich, USA) and sealed to cleavage GST-Tag ...
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bioRxiv - Immunology 2022Quote: ... Tissues were then washed with wash buffer and blocked for 1 h at room temperature with 1x PBS IHC Wash Buffer with Tween 20 and 5% (v/v) normal donkey serum (Sigma-Aldrich). Two antibody panels were prepared ...
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bioRxiv - Immunology 2022Quote: ... The first antibody cocktail was prepared in 1x PBS IHC Wash Buffer with Tween 20 with 5% (v/v) normal donkey serum (Sigma-Aldrich) and 0.5 mM EDTA (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
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bioRxiv - Physiology 2022Quote: ... Samples were attached to rheometer platforms with fibrin glue by applying 5 μL each of 20 mg/mL bovine plasma fibrinogen (341573, Sigma-Aldrich) and 100 U/mL bovine thrombin (T4648 ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 10 min 100% EtOH) and cleared (3 x 5 min) in xylene (247642, CAS: 1330-20-7, Sigma Aldrich). Finally ...
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bioRxiv - Biophysics 2023Quote: ... We blocked the membrane for 1 hour with blocking buffer: 5% bovine serum albumin (BSA, BioShop) in tris-buffered saline (BioShop) with Tween 20 (Millipore Sigma) (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... were blocked with 5% blotting grade nonfat milk (Lab Scientific, #M0841) in tris buffered saline buffer with 0.1% tween 20 (Sigma-Aldrich, #P1379). The following primary antibodies (company ...
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bioRxiv - Cell Biology 2022Quote: ... or mECs per 25 µl droplet were plated overnight as hanging drops in a 20% methylcellulose (9004-67-5, Sigma-Aldrich) in EGM2 mixture ...