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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for 2 Triisopropylsilyl Oxazole 5 Carbaldehyde since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 1% FBS and gluMax along with 5 μM CHIR99021 and 2 μM retinoic acid (R2625, Sigma-Aldrich) for 72 hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Exponentially growing cells were pulse-labeled for 20 minutes with 25 µM 5-iodo-2-deoxyuridine (I7125, Sigma-Aldrich), followed by a second 20-minute pulse with 250 µM 5-chloro-2-deoxyuridine (C6891 ...
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bioRxiv - Neuroscience 2024Quote: 6–8-week-old mice received three intraperitoneal (i.p.) injections of 5-Bromo-2′-deoxyuridine (BrdU, Sigma Aldrich #B9285) at 100 mg/kg (i.p ...
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bioRxiv - Molecular Biology 2024Quote: ... A 3 ml sucrose cushion (10 mM Tris-HCl pH 7.5, 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 µg/ml cycloheximide [Sigma, C4859] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... media was aspirated from cells and replaced with 5 mg/mL 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, #M5655, Sigma Aldrich) solution in base media ...
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bioRxiv - Neuroscience 2024Quote: ... and then in 0.175 M sodium acetate (14.36 mg/ml in ddH2O, pH 6.8, adjusted with glacial acetic acid, 2 × 5 min; Cat# S8750, Sigma-Aldrich). Sections were reacted in 0.5 mg/ml 3 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Glass micropipettes were backfilled with 133 mM of 5- or 6-carboxyfluorescein with a negative charge (-2) (Sigma-Aldrich) diluted in potassium acetate 0.1 M (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were washed and followed by 3-hour incubation with 0.5 mg/mL MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) (Sigma-Aldrich). Cell apoptosis was measured with Annexin V Apoptosis Detection Kit I (BD Pharmingen ...
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bioRxiv - Cell Biology 2024Quote: ... 30 µl of secreted medium and 70 µl of LAAO assay solution (0.1 M Tris-HCl pH 8.0, 5 mM L-leucine, 2 mM OPD (o-phenylenediamine dihydrochloride) (Sigma, P9187), 33.3 µg/ml peroxidase (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: 5-FU and 5’-deoxyadenosine (5’-dAdo) were obtained from Millipore-Sigma Canada Ltd ...
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bioRxiv - Genetics 2022Quote: ... 5’-fluoroorotic acid (5’FOA; Sigma) was used at a concentration of 1 g/L in PMG containing 45 mg/L uracil ...
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bioRxiv - Molecular Biology 2024Quote: ... 5-Fluoroorotic acid (5-FOA; Sigma), Blasticidin (Invivogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5-fluorouracil (5-FU; Sigma, #F6627) was diluted to 10 mM in water and stored at room temperature (RT) ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibodies were washed away with PBS (3x 5 min) whereafter 4’,6-diamidino-2-phenylindole (DAPI, 1:500, Sigma) was added for 10 minutes to localize the cell nuclei ...
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bioRxiv - Microbiology 2021Quote: ... Single-cell suspensions were achieved using a 100 µm cell-stainer (Becton Dickinson) and a 5 mL syringe plunger in RPMI supplemented with 2% heat-inactivated fetal bovine serum (Sigma) and 100 µg/mL DNASE1 (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... accumulation was assessed by measuring the levels of the oxidized form of the cell-permeant 5-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) (Sigma). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... saline or 6-OHDA (5 μg/μL; 2 μL in total, at a rate of 0.5 μL/min, Sigma, Canada) were injected unilaterally in the dorsal striatum ...
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bioRxiv - Immunology 2021Quote: Three- to 4-week-old mice were injected intraperitoneally twice with 0.25 mg 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) 2 hours apart ...
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bioRxiv - Physiology 2022Quote: ... Complete skin was separated from the body and incubated in 2 ml dispase II solution (5 mg/ml in 50 mM HEPES/KOH pH 7.4, 150 mM NaCl; Sigma-Aldrich) over night at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: N2a cells were treated for 2 hours with vehicle or Torin1 and then for 5 minutes with 100 μg/mL of cycloheximide (Sigma). Cells were collected in cold PBS supplemented with 100 μg/mL of cycloheximide and then lysed on ice for 15 min in mammalian lysis buffer (100 mM KCl ...
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bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (5 μM) and propidium iodide (PI) (50 μg/mL) (Sigma-Aldrich, St. Louis, USA) and incubated at room temperature for 30-60 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... or 1000 nM of 2-(4-amino-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-1H-indol-5-ol (PP242; Sigma, P0037), (2 ...
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bioRxiv - Neuroscience 2021Quote: ... These coverslips were left to grow at 37°C and 5% CO2 and were treated with 2 µl Ara-C (Sigma) on day in vitro 1 (DIV1 ...
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bioRxiv - Neuroscience 2020Quote: ... Worms were transferred at the L4 larval stage to NGM plates containing 50 μM 5-fluoro-2’deoxyuridine (FUDR, Sigma). The day of the timed egg laying was considered day 0 for lifespan analysis.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in B2 with 2% nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP, Sigma) until adequate colour development ...
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bioRxiv - Cell Biology 2021Quote: ... which were diluted in the fluorescence dilution buffer (PBS supplemented with 5% FBS and 2% bovine serum albumin) containing 0.1% saponin (Sigma-Aldrich). After extensive washing with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were separated by chromatography by injecting 2-10 μl of sample on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μM, EMD Millipore). Flow rate was set to 150 μl/min ...
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bioRxiv - Biochemistry 2021Quote: ... and PsnB variants (single concentrations at 0.4-2 μM) were co-incubated at 37°C in a buffer containing ATP (5 mM; Sigma-Aldrich), NADH (400 μM ...
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bioRxiv - Cell Biology 2021Quote: ... the rest pieces were digested by shaking in 2 mL of 5% (v/v) FBS in RPMI medium containing DNase I (Sigma) (150 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 30 minutes at 37°C prior to lysis with 2% SDS in PBS containing 5 U/μl benzonase (Sigma). Cell lysates were heated for 10 minutes at 95°C and centrifuged at 21000 x g for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... was performed by intraperitoneal (IP) injection of 0.5 ml bacterial suspension containing 2×107 CFU/ml and 5% (wt/vol) mucin (Sigma-Aldrich). During experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were irradiated using 8 J/m2 UV and labelled for 7 hours with 20 µM 5-Ethynyl-2’-deoxyuridin (EdU) and 1 µM Floxouridine (Sigma). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... both paraformaldehyde and methanol fixed cells are incubated with primary antibody diluted in the antibody dilution buffer (PBS supplemented with 5% FBS, 2% bovine serum albumin and 0.1% saponin (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Transduction was performed at a multiplicity of infection (MOI) of 2 in the presence of 5 µg/ml polybrene (Sigma). Cells were selected with 0.5-2.5 and µg/ml puromycin (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... and then stained for 2 minutes at room temperature using filtered 0.5% Solvent Black 3 (CAS Number 4197-25-5; Sigma 199664) dissolved in 75% ethanol ...
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bioRxiv - Microbiology 2020Quote: A549 cells infected with arboviruses or ACE2-SK-N-SH cells infected with SARS-CoV-2 (MOI=0.05-5) were treated with the RIPK2 inhibitor GSK583 (28) (Sigma-Aldrich) for 24 hours ...
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bioRxiv - Cell Biology 2021Quote: ... but the slides had to be deparaffinized first with the following serial incubations: 2×5 minutes xylenes (Sigma, cat# 534056) twice ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times with PBS and incubated for 2 h with blocking and permeabilization buffer (1% BSA, 5% goat serum, 0.15% saponin (Sigma Aldrich)) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 µl of a solution of 5 mg/mL MTT [3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma Chemical Co.) was added ...
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bioRxiv - Cancer Biology 2021Quote: Dried polar samples were resuspended in 100 μL water and 2 μL were injected into a ZIC-pHILIC 150 x 2.1 mm (5 μm particle size) column (EMD Millipore). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... The matrix consisted of a 9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid (super-DHB, Sigma-Aldrich) at a final concentration of 10 mg/ml dissolved in chloroform ...
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bioRxiv - Systems Biology 2022Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described.62
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bioRxiv - Cell Biology 2022Quote: ... cells were grown on coverslips and incubated at 37°C for 2 hrs with either actinomycin D (5 μg/ml, Sigma) or DRB (50 μg/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were exposed to 5 μM CHIR99021 (Axon) between days 2 and 3 and then supplemented with 100 nM RA (Sigma) until day 5 ...