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Citations for Millipore Sigma :
8401 - 8450 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE and analyzed by WB using anti-Flag (Sigma-Aldrich F3165; 1:7500), anti-RPL3 (Proteintech 11005-1-AP ...
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bioRxiv - Plant Biology 2022Quote: ... Either total or immunoprecipitated proteins were separated on a SDS-PAGE gel and transferred to PDVF membrane (Millipore) for immunoblot analysis using anti-HA (ab18181 ...
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bioRxiv - Systems Biology 2022Quote: The proteins were extracted from the frozen cell pellets by lysing the cells with 1% SDC (Sigma, D6750) in HNN Buffe pH 7.8 (50 mM HEPES ...
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bioRxiv - Physiology 2022Quote: ... by adding 20 μL of magnetic beads (Magna ChIP Protein G Magnetic Beads C #16-662, Sigma-Aldrich) and a combination of two anti BMAL1 antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... the same total protein extracts were incubated with Endoglycosidase H at 37 °C for 3 h (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing the proteins were concentrated with Amicon® Ultra-15 Centrifugal Filters Ultracel®-100K (Merck-Millipore, Burlington ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was filtered through 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA) and stored as 1.0 mL aliquots at −80 °C until crystallization experiments ...
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bioRxiv - Cell Biology 2022Quote: ... An equal 20-40 ug of proteins were separated by SDS PAGE and transferred to PVDF membranes (Millipore). Western blots were performed as standard procedures ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X PPI] and precleared by 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred to a 0.45 μm Immobilon-FL PVDF membranes (IPFL00010; EMD Millipore, Burlington, MA, USA) using the eBlot™ L1 Fast Wet Transfer System (GenScript ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Millipore, IPFL00010). PVDF membrane was masked with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein quantification for each sample was conducted by BCA Assay in accordance with kit instructions (Millipore Sigma 71285M).
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated to 10 mL using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore) and loaded into SnakeSkin Dialysis Tubing ...
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bioRxiv - Biophysics 2024Quote: ... The instrument was calibrated using a protein standard mixture: β-amylase (Sigma-Aldrich, 56, 112 and 224 kDa), and thyroglobulin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated via 4-15% SDS-PAGE electrophoresis and transferred to a PVDF membrane (IPVH 00010, Millipore). Membranes were immersed in blocking solution (5% nonfat milk in 0.1 % Tween 20 in 1X TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The RIG-I protein fractions were pooled and concentrated using a 30 kDa Amicon Ultra filter (Millipore #UFC903008). Lastly ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribonucleoprotein (RNP) complexes (dCas9-gRNA) were formed by mixing the annealed gRNA constructs with dCas9 protein (Sigma-Aldrich) in 2:1 molar ratio (600 nM ...
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bioRxiv - Molecular Biology 2024Quote: ... or with 1-5 μg of the appropriate antibody and then with Protein G Sepharose beads (Sigma, P3296). The following day ...
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bioRxiv - Cell Biology 2024Quote: ... Target proteins were probed using their respective antibodies (Table S1) in 5% Bovine Serum Albumin (BSA, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
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bioRxiv - Microbiology 2024Quote: ... the proteins on the gels were transferred onto an activated polyvinylidene difluoride (PVDF) membrane (Immobilon from Millipore, # IPFL00005) and then blocked with 1X TBS-0.1% Tween-20-5% dry milk for 1 hour at room temperature on a rocker ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing protein were pooled and concentrated in a 10K Amicon Ultra-4 spin concentrator (Millipore 234753) down to 500 µL ...
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bioRxiv - Biophysics 2023Quote: ... Lysate containing FLAG-tagged target protein was purified using ANTI-FLAG M2 Affinity Gel resin (Sigma-Aldrich, USA) and eluted with 3x FLAG peptide (APExBIO ...
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bioRxiv - Microbiology 2023Quote: ... The remaining RNA was combined with 25 μl of Magna ChIP Protein A+G magnetic beads (Merck Millipore) coated in 5μl of anti-m6A antibody (Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... 30-100 μg of protein were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Protran, Sigma-Aldrich). Membranes were blocked in PBS or TBS containing 3% BSA or 5% skimmed milk ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was dialysed against water then concentrated using an Amicon Ultra-Ultracel 5 kDa centrifugal filter (Millipore). Antibodies were raised in two guinea pigs by Eurogentec.
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bioRxiv - Systems Biology 2023Quote: ... Proteins were then separated on 12% SDS PAGE and transferred to a PVDF membrane (Millipore Sigma, 0.2 μM). Membranes blocked with 5% milk were then probed with anti-HA (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing desired protein were pooled and concentrated to 500 μL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
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bioRxiv - Immunology 2023Quote: ... Proteins were then precipitated by addition of 800 µL of ice-cold 55% trichloroacetic acid (TCA) (Sigma Aldrich). Samples were left on ice for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 30 µg of total protein extracts were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). After the nonspecific binding sites had been blocked ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing purified protein were united and concentrated on Amicon-Ultra-15 10 K filtration units (Merck-Millipore, Darmstadt ...
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bioRxiv - Cancer Biology 2022Quote: ... Ras protein level in the pull down was analyzed by by immunoblotting using RAS antibody (Sigma-Aldrich #OP40). Total was lysate was also subjected to immunoblotting to determine RAS expression level as the input.
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bioRxiv - Molecular Biology 2023Quote: ... An equivalent amount of protein lysate was subjected to SDS-PAGE and immunoblotting with antibodies against FLAG (Sigma), V5 (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... Protein was detected using HRP-conjugated anti-mouse secondary antibody (Amersham) and Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... the proteins bound to the Flag antibodies were eluted using 400 µg/mL of 3xFlag peptide (Sigma, F4799) during 30 min at 4°C under agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were then electroblotted to methanol–activated polyvinylidene fluoride (PVDF) membranes (Sigma-Aldrich, Cat. No. IPVH00010). Upon transfer ...
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bioRxiv - Cell Biology 2023Quote: ... Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore, IPVH00010). The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight ...
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bioRxiv - Cell Biology 2023Quote: ... biotinylated proteins were selectively eluted by incubating the beads in 100 µL of 5 mM dithiothreitol (DTT, Sigma), 5% SDS ...
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bioRxiv - Immunology 2023Quote: ... at 4°C overnight and then with protein A agarose/salmon sperm DNA slurry (Millipore, Cat# 16-157) at 4°C for 1h ...
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bioRxiv - Plant Biology 2023Quote: ... The eluted protein was concentrated to 1 mg/ml using Amicon Ultra 10 kDa MWCO filters (Millipore Sigma). Size-exclusion chromatography was performed using a Superose 6 10/300 GL column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were separated on denaturing polyacramide gels before transfer to a 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were extracted from 400,000 to 500,000 cells with RIPA buffer with the addition of proteinase inhibitor (Sigma) and phosphatase inhibitors (Sigma) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sized proteins were concentrated to >50 mg mL-1 using 10 kDa molecular weight cut-off concentrators (Millipore), flash frozen on liquid nitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-glial fibrillary acidic protein (GFAP) antibody (dilution; 1:100, Sigma-Aldrich (G6171), St ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was performed on total-protein lysate using 1 μg of rabbit polyclonal TTC29 antibody (HPA061473, Sigma-Aldrich) and 15 μl of Protein A/G magnetics beads (Bio-Ademtec ...
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bioRxiv - Microbiology 2023Quote: ... the eluted proteins were concentrated in an Amicon®Ultra-15 Centrifugal Filter Unit (Millipore; 10 kDa cutoff) and incubated with the SUMO protease Ulp1 (Ulp1:His-SUMO-tH2 = 1:500 w/w ...
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bioRxiv - Microbiology 2023Quote: ... and cells were probed using primary antibodies to VZV proteins IE62 (mouse mAb; EMD Millipore MAB8616; 1:200), capsid-ORF23 (polyclonal rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore; 05317) using a wet blot transfer system (BioRad) ...