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Citations for Millipore Sigma :
8101 - 8150 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the Akt/mTOR phosphoprotein magnetic bead panel 11-plex 96-well plate assay (Merck Millipore, Darmstadt, Germany). This assay uses paramagnetic beads (6.5 μm diameter ...
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bioRxiv - Developmental Biology 2022Quote: ... Total amount of glucose was determined by adding 100 μl of glucose (HK) assay reagent (Sigma, G3293). Plate was incubated for 15 min ...
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bioRxiv - Immunology 2022Quote: ... culture medium was removed and cells were washed once with assay medium consisting of DMEM (Sigma-Aldrich), pH buffered at 7.35 ...
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bioRxiv - Cell Biology 2022Quote: Assessing CFTR function was performed by forskolin-induced organoid swelling assay (2hr with 5μM Forskolin (Sigma-Aldrich)) as described (69) ...
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bioRxiv - Biophysics 2022Quote: For the initial thermal aggregation assays 550 nM citrate synthase from porcine heart (Sigma-Aldrich C3260-5KU) in a 1:2 protein:DNA strand concentration (1.1 µM DNA ...
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bioRxiv - Cell Biology 2022Quote: ... LATS1 kinase was immunoprecipitated from 500 µg of protein lysates and subject to Oxyblot assay (#S7150, Millipore) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Time course assay coverslips were incubated with mouse anti-acetylated α-tubulin (1:2000, Sigma Aldrich, T6793) and rabbit anti-ARL13B (1:800 ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged for 15 min at 20 000 g and protein concentration was assessed using Bradford assay (Sigma). 50 µg of protein for each sample was transferred in fresh tubes ...
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bioRxiv - Physiology 2024Quote: ... Immediately prior to the assay the media was changed to glycolytic stress test medium (Sigma Aldrich D5030) supplemented with 143 mM NaCl and 2mM L-glutamine (Gibco 25030 – 081) ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was collected and total protein content determined with a BCA Protein Assay (Sigma, Poole, UK). A total of 20μg protein extract for each sample was separated by electrophoresis on Nu-PAGE™ 4-12% ...
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bioRxiv - Immunology 2024Quote: ... the culture supernatant was used for cytokine quantification by a 32-plex Luminex assay (Milliplex, Millipore Sigma) in accordance with the manufacturer’s recommendations.
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bioRxiv - Synthetic Biology 2023Quote: Glutamate and maltose concentrations were determined using enzymatic colorimetric assays purchased from Sigma-Aldrich (St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was retrieved and protein concentration was measured by BCA protein assay (Novagen, (Smith et al. 1985). Protein concentrations were adjusted in all the samples to 800 µg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... and differentiated 3T3-L1 adipocytes (2.5x103 cells/well) in DMEM assay medium composed of DMEM (Sigma #5030) supplemented with 31.6 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The effect of KLF5 knockdown on cell viability/proliferation was measured by the MTT assay (Sigma M2128) at 72hrs (or at the indicated time point ...
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bioRxiv - Immunology 2023Quote: ... Cell concentrations and viability were determined using the ViaCount assay using a Guava easyCyte machine (Millipore-Sigma).
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bioRxiv - Cancer Biology 2023Quote: ... Its effect was measured using the 3-(4,5-dimethylthiazol-2yl)-diphenyltetrazolium bromide (MTT) colorimetric assay (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cholesterol depletion and supplementation assays were performed in the presence of both 40 μM pravastatin (Sigma-Aldrich) and 50 nM of Shh-N for 24 h ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were glucose-starved for 60 minutes in seahorse assay base media containing 2 mM glutamine (Sigma). Glycolysis was measured upon glucose injection (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Cell concentrations and viability were determined using the ViaCount assay using a Guava easyCyte machine (Millipore-Sigma).
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bioRxiv - Plant Biology 2023Quote: ... and subjected to western blot assay using HRP-conjugated anti-FLAG M2 (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Molecular Biology 2023Quote: ... Protein lysates for DNA-RNA hybrid pull-down assays were treated with 10 µL RNaseA (Sigma # R6148) or with 10 µL RNaseH (NEB # M0297S ...
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bioRxiv - Biophysics 2023Quote: ... the assay buffer for the standards and the ALP substrate solution (0.04 g phosphatase substrate (Sigma Aldrich) in 10 mL alkaline buffer solution (1.5 M ...
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bioRxiv - Neuroscience 2023Quote: ... Flies used in optogenetic assays were reared on food containing 0.25mM all-trans-retinal (Sigma-AldrichSigna-Aldrich) in darkness ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 20 μl of imaging buffer (assay buffer supplemented with the protocatechuic acid (PCA, Sigma Aldrich)/protocatechuate 3,4-dioxygenase (PCD ...
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bioRxiv - Systems Biology 2023Quote: ... membrane permeability was quantified for each assay by addition of FITC-dextran (4000 MW, Sigma Aldrich 46944) (0.5 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... The assay deep-well plate was sealed with an AeraSeal breathable membrane (Sigma-Aldrich, cat. No. A9224) and incubated at 37°C anaerobically for 5 h ...
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bioRxiv - Biophysics 2024Quote: ... malachite green assay (Malachite green oxalate salt, Sigma-Aldrich, cat M6880, Ammonium molybdate tetrahydrate, Sigma, cat 09878) was performed ...
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bioRxiv - Molecular Biology 2024Quote: ... Hepatic triglycerides were measured using the Triglyceride Quantification colorimetric-/fluorometric assay (MAK266, Sigma-Aldrich, St. Louis, US). A hematoxylin and eosin (HE ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability was assessed using colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay following by the manufacturer’s instructions (Sigma). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: The cell death detection kit (In Situ Cell Death Detection Kit, Sigma-Aldrich) was used to perform TUNEL analysis according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Trichrome stain (Masson) kit and senescence cells histochemical staining kit (Sigma-Aldrich, Germany); DAB substrate kit (Vector Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Proteins were loaded on PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Samples were heated at 100 °C for 10 min in a 4X loading buffer ...
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bioRxiv - Immunology 2021Quote: ... Arginase activity in the CECs or splenocytes lysates and cell supernatant was determined using Arginase Activity Assay (Sigma) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Plaque assays were undertaken in vero E6 cells using a 1.2% (w/v) microcrystalline cellulose overlay (Sigma-Aldrich). Cytokines/inhibitors were used at the following concentrations ...
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bioRxiv - Cell Biology 2022Quote: Migration assays were performed in transwell polycarbonate membrane cell culture inserts with a 5µm pore (Corning, Sigma-Aldrich) using 1.25×105 hTERT and primary MSCs ...
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bioRxiv - Cell Biology 2019Quote: Bulk kinetic dextran release assays were performed with OG-LUV loaded with fluorescein-dextrans (70 kD)(Sigma; FD70S) or cascade blue (CB)-dextrans (10 kD)(Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Annexin V-FITC apoptosis assay was carried out as described in manufacturer’s protocol (APOAF, Sigma. St. Louis, MO). After transfected with Npac RNAi or control RNAi plasmid in 6-well dishes and selected for 4 days ...
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bioRxiv - Plant Biology 2019Quote: ... The MUG assay buffer is same as that of the extraction buffer supplemented with 1 mM MUG (Sigma). The reaction was incubated at 37°C water bath for 20 min ...
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bioRxiv - Bioengineering 2019Quote: ... GAG quantities were determined using a modified dimethyl methylene blue (DMMB) assay.[72] Shark chondroitin sulfate (Sigma; C4348) was included as a standard ...
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Cancer Biology 2019Quote: ... The Duolink® in situ proximity ligation assay (PLA) was performed according to the manufacturer’s instructions (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... Then the medium was collected and replaced with stimulation medium (basal assay medium containing 100 µM histamine, Sigma) for 20 min ...
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bioRxiv - Bioengineering 2020Quote: ... GAG was quantified using a modified dimethyl methylene blue (DMMB) assay [42] with Shark chondroitin sulfate (Sigma, C4348) as a standard ...
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bioRxiv - Biochemistry 2021Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma-Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Biochemistry 2020Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Plant Biology 2021Quote: ... The stress assays were performed on CM plates (Holliday 1974) supplemented with 750 µM calcufluor white (Sigma-Aldrich), 3 mM hydrogen peroxide (H2O2) ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed a final time and the assay was developed using the SigmaFast pNpp reagent (Sigma Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... and for flippase specific assays in the presence of an ATP regeneration system (0.5 mM Phosphoenolpyruvate (Sigma-Aldrich) and 100 U ml−1 pyruvate kinase (Sigma-Aldrich)) ...