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Citations for Millipore Sigma :
8001 - 8050 of 10000+ citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies included: 1:100 Alexa Fluor 488-conjugated anti-α-tubulin (mouse monoclonal DM1A, Millipore-Sigma #16-323), 1:200 rabbit anti-phosphorylated histone H3 (S10 ...
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bioRxiv - Molecular Biology 2019Quote: ... 927-40000) and subsequently incubated with primary antibody overnight at 4°C (1:1000 anti-CTCF C-terminus Millipore 61311 and 1:2000 anti-TBP Abcam ab51841 ...
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bioRxiv - Cell Biology 2020Quote: ... incubated overnight at 4 °C in primary antibody: rat anti-Laminin-α2 (1:250, Cat. No L0663, Sigma-Aldrich), rabbit anti-Collagen Type I (1:250 ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were incubated with monoclonal mouse ANTI-FLAG® M2 antibody (Sigma-Aldrich, F1804-50UG, dilution 1:100) overnight at 4°C and then with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Blot was stripped and re-probed for total receptor using HRP conjugated anti-FLAG M2-antibody (Sigma, 1: 5000). The signal was normalized with respect to total receptor and quantified using ImageLab software (BioRad).
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bioRxiv - Biochemistry 2020Quote: ... Analysis of binding was conducted by western blot with the following antibodies: anti-FLAG (Sigma Aldrich, A9469, 1:1000), anti-tubulin antibodies E7 (DSHB ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed with PBST and incubated with a goat anti-human Fc−peroxidase antibody diluted 1:5,000 (Sigma) in blocking buffer for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were then washed and incubated with a 1:5,000 dilution of peroxidase-conjugated anti-human Fc antibody (Sigma) for 1 hr ...
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bioRxiv - Cell Biology 2022Quote: ... Dilutions of antibodies used for immunoblotting were as follows: mouse anti-Flag at 1:7,500 (clone M2; Sigma-Aldrich), mouse anti-Myc at 1:1000 (9E10 ...
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bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with primary antibody in blocking solution (1:500 mouse anti-gamma tubulin (Sigma Aldrich, GTU-88), 1:3000 mouse anti-ARL13B (Biolegend ...
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bioRxiv - Cell Biology 2019Quote: ... 3% goat serum) and then incubated with mouse anti-tropomyosin-5 antibody (1:200; clone 2G10.2, Millipore, Burlington, MA) which recognizes Tpm3.1 and Tpm3.2 ...
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bioRxiv - Bioengineering 2019Quote: ... The antibodies used were mouse anti-human HSA (1:500; clone HSA-11, A6684; Sigma Aldrich; St. Louis, MO) and 10 nm colloidal gold-conjugated goat anti-mouse IgG (1:40 ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were incubated overnight at 4 °C with monoclonal anti-FLAG M2 antibody (1:1000, F1804, Sigma-Aldrich, Germany) and polyclonal rabbit anti-H3K9me3 antibody (1:100 ...
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bioRxiv - Developmental Biology 2019Quote: ... proteins were transferred to a PVDF membrane and immunoblotted with an anti-phosphotyrosine antibody (1:1000 dilution, Upstate/Millipore) for chemiluminescence detection.
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bioRxiv - Cell Biology 2019Quote: ... Flow cells were first incubated with 30 μl of 1:100 dilution of monoclonal anti-β-Tubulin antibody (Sigma) for 10 minutes in a humidified chamber ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Developmental Biology 2019Quote: The primary antibodies and concentrations used were: mouse anti-alpha tubulin (1:500; Sigma-Aldrich, Inc. Cat.# T9026. RRID:AB_477593). Secondary antibodies are listed in the Key Resources table.
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Systems Biology 2020Quote: ... Dll1 was detected with a 1:50 dilution of rat anti-Dll1 antibody (PGPM-1F9, MABN2284 from Sigma-Aldrich). After incubation the cultures were washed three times with 1% Triton-X-100 ...
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bioRxiv - Microbiology 2020Quote: ... Actin was detected using anti-actin antibody (1:2000 in 5% BSA/TBS-T, Clone AC-74, Sigma-Aldrich). Antibodies were detected using Goat anti-Rabbit IgG-HRP conjugate (1:10000 ...
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bioRxiv - Microbiology 2020Quote: ... The polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with appropriate antibodies: anti-Arp (dilution 1:1,000; Sigma); anti-FHOD1 (dilution 1:1,000 ...
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bioRxiv - Pathology 2021Quote: ... 96-well plate was coated with 1 μg/ml monoclonal anti-lubricin antibody clone 9G3 (Merck Millipore, Billerica, MA) in TBS at 4°C over-night ...
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bioRxiv - Genetics 2021Quote: ... a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374, used at 1:10,000 for the Western blot analyses, Merck Millipore); a rabbit anti-LC3B polyclonal antibody (#2775 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used for assessing the neuronal localization of S100a4 were anti-NeuN (MAB377, Sigma-Aldrich, dilution 1:200) and anti-S100a4 (ab41532 ...
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bioRxiv - Neuroscience 2020Quote: ... or AAVretro-EF1a-Cre primary antiserum (1:100 mouse-anti-Cre Recombinase antibody, clone 2D8, Millipore Sigma Cat# MAB3120) mixed with donkey serum ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 293T or AL1 cells were incubated for 3 hours with anti-Myc mouse monoclonal antibody (Sigma-Aldrich; 1:500), and secondary staining was performed using anti-mouse AlexaFluor568 antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... PPTg sections were incubated with a goat anti-ChAT primary antibody (1:100, Millipore, catalog# AB144P-200UL, lot# 2854034) for 60 hours at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with 1:2000 alkaline phosphatase-conjugated anti-DIG antibodies (Sigma/Roche, 11093274910). After washes and prior to development ...
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bioRxiv - Cell Biology 2020Quote: ... H4S1 phosphorylation was detected using the anti-phospho histone H4/H2A S1p antibody at 1:1000 (Upstate/Merck-Millipore); sf-GFP-Sps1 was detected using JL-8 anti-GFP antibodies (Takara/Clontech ...
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bioRxiv - Neuroscience 2021Quote: ... were labelled by calcitonin gene-related peptide (CGRP) immunoreactivity using a monoclonal anti-CGRP antibody (1:5000; Sigma #C7113) raised in mouse ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2020Quote: ... A 5 μm section was deparaffinized and stained with anti-MAP2 antibody (Sigma-Aldrich, 1:1000 dilution, Cat# M4403) (the details of this procedure are described elsewhere (Shichita et al ...
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bioRxiv - Microbiology 2021Quote: ... spirochetes were incubated for 60 min with goat anti-mouse gold-labeled antibody at 1:30 dilution (G7652; Sigma). The grid was further washed ...
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bioRxiv - Cell Biology 2021Quote: ... the following primary antibodies were used at the indicated dilutions: mouse anti-γ-tubulin 1:500 (T5326; Sigma Aldrich), rabbit anti-mCherry 1:500 (ab167453 ...
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bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-acetylated tubulin (clone 6-11-B1, Sigma-Aldrich, T6793; 1:1,000), mouse-anti beta-actin (Sigma ...
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bioRxiv - Biochemistry 2020Quote: All antibodies were from commercial sources: anti-FLAG M2 peroxidase was obtained from Sigma Aldrich (F4042, 1:1,000 dilution), anti-HIF1α was from BD Biosciences (610958 1:1,000 dilution) ...
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bioRxiv - Genetics 2020Quote: ... blots were incubated with the following antibodies: monoclonal mouse anti-GFP (Sigma, MO; clone 7.1 and 13.1, 1:1000), mouse anti-Tubulin (DHSB ...
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bioRxiv - Microbiology 2022Quote: ... followed by 4x 8 min in PBS and then incubated in PBS-5% dried semi-skimmed milk with 1:10,000 anti-mouse HRP-linked secondary antibody (A9044, Sigma) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Secondary incubation was performed with 1:20 000 dilution of HRP-conjugated anti-human IgG antibody (Sigma Aldrich SAB701283) for 1 hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 3-5 times with 1x TBST for a total of 20 min and then incubated for an hour with 1:3,000 dilution of secondary antibody (goat anti-mouse alkaline phosphatase conjugate, Sigma). The membrane was washed again with 1x TBST 3-5 times for a total of 20 min and developed using 5-bromo-4-chloro-3-indolylphosphate (BCIP)/ nitro-blue tetrazolium (NBT ...
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bioRxiv - Pathology 2022Quote: ... and incubated in 1:100 mouse anti-cytokeratin CAM5.2 monoclonal antibody (item 452M-94,Sigma-Aldrich, St. Louis, MO) or 1:1000 anti- CD163 monoclonal antibody (Novus Biologicals ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were washed and then treated with a 1:1000 dilution of mouse anti-FLAG antibody (clone M2, Sigma) for 60 minutes then washed 3x in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... after RNAscope detection of C3 some slides were co-labelled with an anti-GFAP antibody (1:400, mab360, Millipore) according to the immunohistochemistry methods.