Labshake search
Citations for Millipore Sigma :
8001 - 8050 of 10000+ citations for 6 CHLORO 4' METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then refixed in 4% PFA for 5 min at RT then incubated in 2xSSC (Sigma) for 2 x 5 min RT ...
-
bioRxiv - Biophysics 2020Quote: A polyethylene glycol (PEG) solution (w/v, 6%) was made by dissolving PEG powder (Molecular weight 300,000; Sigma-Aldrich Co Ltd, MO, US; product of USA) in Milli-Q water at 50 °C with magnetic stirring for 8 h (25) ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then blocked in 1% BSA for 20 minutes before being incubated in a primary antibody against acetylated α tubulin (1:4000 dilution, clone 6-11B-1, Sigma-Aldrich, St. Louis, MO, USA), GFP (1:1000 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Antibodies used in this study were: anti-acetylated α-tubulin (Antibody registry ID: AB_477585, clone 6-11B-1, mouse IgG2b, Sigma, diluted 1:250 in blocking solution), anti-SV2 (AB_2315387 ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... They were then placed under isoflurane anesthesia (1.5% in 30% oxygen and 70% nitrous oxide) in a stereotaxic apparatus (David KOPF Instruments, Tujunga, CA, USA) and received injection of 6-OHDA (Sigma-Aldrich Co., St. Louis, MO, USA) at four sites targeting the dorsomedial striatum bilaterally (AP ...
-
bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO], 20 ng/ml bFGF [Sigma], and 20 ng/ml EGF [Sigma]) before collection for single-cell analysis ...
-
bioRxiv - Plant Biology 2021Quote: The dried samples were derivatized for 2 hours at 37 °C in 50 μI of 20 mg ml-1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
-
bioRxiv - Immunology 2020Quote: Perigonadal white adipose tissue (WAT) was digested in 6 ml of Collagenase II buffer (Collagenase II 2mg/ml; Sigma Aldrich, USA, 0.5 % BSA in 1X PBS) at 37 °C with shaking at 200 r.p.m for 20 min ...
-
bioRxiv - Plant Biology 2022Quote: ... and the residue was derivatized for 120 min at 37 °C (in 40 μl of 20 mg ml−1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37 °C with 70 μl of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
-
bioRxiv - Neuroscience 2023Quote: ... animals received the first of 6 injections of either vehicle (propylene glycol) or progesterone (>99% progesterone, purified from rats, Sigma-Aldrich Co. St. Louis, MO, USA) at a dose of 4 mg/kg (Shear et al. ...
-
bioRxiv - Physiology 2023Quote: Intracellular succinate concentration was measured in cells under normoxic conditions and after exposure to 1 and 6 h hypoxia using a commercial colorimetric assay (Sigma catalog # MAK184, St. Louis, MO, USA) according to manufacturer instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... and the residue was derivatized for 120 min at 37°C (in 40 μl of 20 mg ml-1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37°C with 70 μl of N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
-
bioRxiv - Biophysics 2023Quote: Cell adhesion on collagen fibers (Fig. 7a -d) is prevented either by adding pluronic surfactant (1 vol% in PBS, Sigma-Aldrich, cat. no. 9003-11-6), or by calcium ion chelation with 5 mM ethylenediaminetetraacetic acid (EDTA ...
-
bioRxiv - Immunology 2024Quote: ... we used inhibitors of ERK1/2 (Trametinib, SelleckChem, Catalog # S2673), p38 (Losmapimod, Selleckchem Catalog #S7215), NF-kB (BAY11-7082, Catalog # S2913) and JNK inhibitor (Millipore-Sigma Catalog # CAS 129-56-6) 2 hr before stimulation with R848 or Poly I:C until sample collection at 8 or 24hrs for mRNA or protein levels of antiviral and inflammatory cytokines and chemokines.
-
bioRxiv - Cancer Biology 2021Quote: ... Lentivirus containing supernatants were harvested and transduced into AML cell lines with 4 μg/ml sequabrene (Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2021Quote: ... Sonicated chromatin was incubated at 4°C overnight with either an Anti-H3K4me3 (Millipore 04-745; 1 µg) or Anti-H3K27me3 (Millipore 07-449 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Membranes were probed overnight at 4°C with mouse anti-BRAF [clone 3C6] (Sigma-Aldrich, cat# WH0000673M1; RRID:AB_1840330) at 2.5 µg/ml dilution ...
-
bioRxiv - Cancer Biology 2021Quote: ... The cell lines were regularly passaged every 3-4 days by dissociation with Trypsin-EDTA 0.25% (Sigma Aldrich). The cell lines were regularly subjected to monthly screening for mycoplasma infection ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were incubated in presence of 2mM 5FUrd for 20 minutes followed by fixation in 4% FA (Sigma). They were permeabilized with triton X100 (0.5 % in PBS ...
-
bioRxiv - Immunology 2021Quote: ... Mice also received four doses of a 50mg/mL solution of 4-hydroxy-tamoxifen (Millipore Sigma, cat. T176) in DMSO (AmericanBIO ...
-
MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Cells were fixed with 4% paraformaldehyde for 15 min and afterwards permeabilized/stained with DAPI (D9542, Sigma-Aldrich) in 1xPBS containing 0.05% Triton X-100 for 10 min ...
-
bioRxiv - Cell Biology 2020Quote: ... HL-60 cells were infected in complete medium supplemented with 4 µg/ml polybrene (Sigma TR-1003-G) and selected with 1 µg/ml puromycin (Gibco A1113803 ...
-
bioRxiv - Biophysics 2020Quote: ... The cell pellets were suspended in 5 ml pure water at 4 ℃ with 0.4 % TritonX-100 (Sigma-Aldrich), 2 μl DNAase Ⅰ(Sigma Aldrich ...
-
bioRxiv - Developmental Biology 2021Quote: Dechorionated 4 ss embryos were treated with 30 μM KN-93 (Wako, Japan) or DMSO (Sigma Aldrich, Germany) in 1x Yamamoto’s Ringer Solution at 28 °C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... equilibrated with 0.01 M HEPES/0.5mM EDTA (pH 7.4) and eluates were concentrated using Amicon Ultra-4 Centrifugal Filter Units (Millipore).
-
bioRxiv - Developmental Biology 2021Quote: ... we treated Tg(ins:Flag-NTR);Tg(ubi:Switch);Tg(drl:CreERT2) zebrafish embryos with 10 μM 4-OHT (Sigma-Aldrich) in E3 medium in 90-mm Petri dishes ...
-
bioRxiv - Biophysics 2022Quote: ... HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl] ethanesulfonic acid) was purchased from EMD Millipore (Billerica, MA USA) and NaCl (sodium chloride ...
-
bioRxiv - Cell Biology 2021Quote: ... The sections were stained with 4% aqueous uranyl acetate and citrate mixture (Sigma Chemical Co., St. Louis, USA).
-
bioRxiv - Neuroscience 2021Quote: ... LRRK2 was immunoprecipitated from 4 mg of lysate using 20 μl anti-FLAG-M2 agarose beads (Sigma, #A2220) for 2 h in rotation at +4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... a click chemistry reaction cocktail was added to the cells (100 mM Tris HCl pH 7.6, 4 mM CuSO4, 2.5 µM azide-fluor488 (Sigma), 100 mM Sodium ascorbate (Sigma) ...
-
bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated overnight at 4°C with one of the following primary antibodies: FLAG (Sigma-Aldrich, F3165), γH2AX (Millipore ...
-
bioRxiv - Immunology 2021Quote: ... was cultured overnight shaking at 37oC and subcultered to an optical density (A600) of 0.4-0.8 for 4 hours in yeast extract peptone dextrose (YEPD; Sigma) medium ...
-
bioRxiv - Molecular Biology 2021Quote: ... and incubated at 4°C overnight on a rotating mixer in the presence of Protease Inhibitor Cocktail (Sigma). Then affinity resin was washed consecutively with 1 mL each of the following solutions ...
-
bioRxiv - Microbiology 2019Quote: ... Microspheres were washed with 50 mM MES potassium salt (4-morpholineethane sulfonic acid, Sigma Aldrich, St. Louis, MO) pH 5.0 to 10000 beads/µl ...
-
bioRxiv - Neuroscience 2019Quote: ... Recording pipettes (tip resistance 4-6MΩ) were filled with intracellular solution containing 120mM potassium D-gluconate (G4500, Sigma), 1mM MgCl2 ...
-
bioRxiv - Bioengineering 2020Quote: ... was used at the concentration 4 μM and the ROCK inhibitor Y27632 (Sigma-Aldrich, cat. No. Y0503,10mM stock) at 10 μM ...
-
bioRxiv - Microbiology 2019Quote: ... incubated with 10 μM of the cysteine protease inhibitor l-transepoxysuccinyl-leucylamido-(4-guanidino)butane (E64, Sigma-Aldrich) for up to 6 hours under standard culture conditions until at least 50 % of schizonts had developed into parasitophorous vacuole enclosed membrane structures (PEMS ...
-
bioRxiv - Plant Biology 2020Quote: ... RNA was extracted from 4-week-old Arabidopsis leaves using the Spectrum plant total RNA kit (Sigma-Aldritch) according to manufacturer’s instructions.
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... We then injected the pectoral muscle of 4 females with 34 mg mitotane (Sigma-Aldrich, Cat. No. 25925), dissolved in 400 µl sterile peanut oil (Acros Organics ...
-
bioRxiv - Systems Biology 2020Quote: ... and cells were resuspended in 7 mL of ice-cold 4% formaldehyde (F8775, Millipore Sigma, St. Louis, MO) in PBS (P0195 ...
-
bioRxiv - Cell Biology 2020Quote: ... membranes were probed at 4°C with primary antibodies: polyclonal anti-UCP1 (1:500, Sigma-Aldrich cat#U6382), and anti-actin (1:10000 ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... 10 μl of 4 mg/ml 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB, Sigma-Aldrich, St. Louis, MO, USA) in reaction buffer pH 8 was added to wells containing samples and standards ...
-
bioRxiv - Biochemistry 2019Quote: ... and rotated overnight at 4 °C in the presence of 45 µl anti-FLAG M2 affinity gel (Sigma). The following morning ...
-
bioRxiv - Immunology 2020Quote: ... Mice also received two consecutive doses of a 50mg/mL solution of 4-hydroxytamoxifen (Millipore Sigma, cat. T176) in DMSO (AmericanBIO ...
-
bioRxiv - Neuroscience 2019Quote: ... cells at 3–4 DIV were incubated with culture medium containg 0–0.8 mM methylmethanesulfonate (MMS, Sigma, 129925) or 0–8 µM etoposide (Sigma ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were transfected with a total of 300 ng DNA (per well of a 4-well 3.5 cm dish) using 0.75 µl Genejuice (Merck Millipore) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2019Quote: ... 700-1000 µg total protein was incubated overnight at 4°C with 1:100 anti-FLAG (Sigma, F1804) or anti-Myc (Sigma ...
-
bioRxiv - Genomics 2020Quote: ... Cells were treated with EGF (0.1μg/mL) and concurrently labelled with 500μM 4-thiouridine (4sU; Sigma Aldrich T4509) for 15 minutes ...
-
bioRxiv - Cell Biology 2019Quote: ... and collected by centrifugation at 400 g for 2 min before fixation with 4% Paraformaldehyde (PFA, Sigma-Aldrich) at room temperature for 10 min ...
-
Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: ... The biotinylated extracts were incubated overnight at 4 °C with 100 μl of streptavidin-agarose suspension (S1638, Sigma). The next day ...