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Citations for Millipore Sigma :
7951 - 8000 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The reactions were then incubated at 37 °C for 60 min and stopped by adding 1 µl of stop buffer (0.4% SDS, 30 mM EDTA, and 6% glycerol) and 1µl of proteinase K (20 mg/ml, Sigma) into the reaction for another 10 min incubation at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... IVT transcripts were analyzed by 6% TBE polyacrylamide gel electrophoresis and stained by acridine orange (AO) (Sigma Aldrich). Fluorescence gel images were obtained by a ChemiScope 6000 Imaging System (CLINX) ...
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bioRxiv - Biochemistry 2023Quote: ... 10% (w/v) sucrose] supplemented with 100 µM concentration of the membrane-impermeant fluorophore 5(6)-carboxyfluorescein (Sigma) at 45 °C to form liposomes with a final lipid concentration of 8 mM ...
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bioRxiv - Cell Biology 2024Quote: ... 6-Diazo-5-oxo-L-norleucine (DON) was used at final concentration of 2,5µM (Sigma Aldrich, ref. D2141). 2-Desoxy-D-glucose (2-DG ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) and mounted in Mowiol (Sigma-Aldrich, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... we used 2’,7’-Bis (2-carboxyethyl)-5 (6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) (Sigma-Aldrich, Darmstadt, Germany). Cells were starved overnight ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed with 3.6% PFA after 6 days and plaques were counted manually after staining with 0.1% crystal violet (Sigma).
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bioRxiv - Biophysics 2024Quote: ... 90,000 U2OS cells/well were seeded in 6-well with 8 ug/ml polybrene (Millipore #TR-1003-G) addition to the growth medium and infected with 4ul of lentiviral particle containing supernatant ...
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bioRxiv - Cancer Biology 2024Quote: ... 6 sec) and then vapor silanized with Trichloro (1H,1H,2H,2H-perfluoro-octyl) silane (Si; Sigma, 448931). To make the 1st PDMS ...
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bioRxiv - Microbiology 2024Quote: ... The plate was then rinsed with PBS and counter-stained with 4′,6-diamidino-2-phenylindole DAPI (Sigma) for 15 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cells were washed with acidic MEM buffer (containing 15 mM HEPES with final pH 6.7-6.8 adjusted with acetic acid (Millipore Sigma)) after 45-60 minutes of calcium phosphate precipitate incubation to remove residual precipitates.
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bioRxiv - Biophysics 2021Quote: ... 2 μL of 100 mM 5,5′-dithiobis-(2-nitrobenzoic acid) (Sigma Aldrich, dissolved in 50 mM NaH2PO4, pH 7.5) was immediately added and fully mixed by pipetting before incubating at room temperature for 20 min ...
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bioRxiv - Biophysics 2021Quote: ... An oxygen scavenger system [50 or 100 nM protocatechuate 3,4-dioxygenase (OYC Americas) and 2 mM protocatechuic acid (Sigma)] and 2 mM Trolox were added to suppress Cy5 blinking and photobleaching29 ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Molecular Biology 2020Quote: ... the fresh cXEN derivation medium was changed to a solution of standard XEN medium supplemented with 0.01 μM all-trans retinoic acid (Sigma) dissolved in DMSO plus 10 ng/ml activin A ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were resuspended in 800 µl 20% (w/v) trichloroacetic acid and lysed with 0.5 mm glass beads (Sigma) in a FastPrep FP120 (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were resuspended in 1600 µl 20% (w/v) trichloroacetic acid and lysed with 0.5 mm glass beads (Sigma) in a FastPrep FP120 (Thermo ...
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bioRxiv - Cell Biology 2019Quote: ... glass-bottom dishes (IWAKI, Shizuoka, Japan) were treated with 2% acetic acid (WAKO) and 0.2% 3-(Trimethoxysilyl)propyl methacrylate (SIGMA) in 80% ethanol for 2 min ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Peptides were separated using a 250 min gradient ranging from 2 % to 90 % mobile phase B (mobile phase A: 2% acetonitrile [Scharlab S.L.], 0.1 % formic acid [Sigma-Aldrich] ...
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bioRxiv - Immunology 2020Quote: ... mosquitoes were kept at 26.0°C (± 0.5°C) in an ultrasonic humidity cabinet and provided with 8 % Fructose and 0.05 % 4-Aminobenzoic acid (both Sigma) feeding solution from this point forward ...
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bioRxiv - Developmental Biology 2020Quote: ... digestion reaction was stopped by reducing the pH < 2 through the addition of trifluoroacetic acid (TFA, ≥ 99.0 %, Sigma-Aldrich) to a final concentration of 0.4 % (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µL of the reaction was removed and quenched by adding 60 µL of 0.75 % formic acid (FA, Sigma) and 0.25 mg/mL porcine pepsin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and performed western blotting analysis.Western blotting: Protein concentration of lysates was determined with Bicinchoninic Acid Kit (#BCA1, Sigma Aldrich). Equal amount of proteins was loaded on the poly-acrylamide gels ...
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bioRxiv - Neuroscience 2021Quote: ... Kyoto University) and mouse anti-glutamic acid decarboxylase of 67 kDa (GAD67; 2 μg/mL; MAB5406, Millipore, RRID: AB_2278725) in Triton-PBS containing 1% v/v donkey serum (Jackson Immunoresearch ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 g/l NaCl) supplemented with 1.7 g/l yeast nitrogen base without NH4Cl and amino acids (Sigma Y1251). 1 g/l 15NH4Cl and 4 g/l 13C-glucose were supplemented for 15N and 13C labelling respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Aliquots of stock solutions were complexed with fatty-acid–free BSA (10% solution in 150 mM NaCl; Sigma-Aldrich) by stirring for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were acid-washed and treated with fibronectin (10 μg/ml for 30 min at 37°C, Sigma F4759) to enhance cell spreading and adhesion ...
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bioRxiv - Biochemistry 2019Quote: ... The peptide digest was dissolved in 21 μL of LC-MS grade water containing 0.1% formic acid (F0507-100ML, Sigma). The digest was filtered with a 0.65 µm pore-size micro-centrifuge filter before it was loaded to an HPLC auto-injection sample.
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bioRxiv - Cell Biology 2019Quote: ... were coated with a 100 µL solution of 20 µg/mL porcine fibronectin (prepared from whole blood) and 0.4 mg/mL bovine serum albumin (BSA, endotoxin-free, fatty acid free; A8806, Sigma) dissolved in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Evolutionary Biology 2019Quote: All chemicals used to stain for tartrate resistant acid phosphatase activity (Hadler et al., 2008) were purchased from Sigma. TRAP Basic Incubation Medium is comprised of 9.2 g sodium acetate anhydrous ...
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bioRxiv - Cell Biology 2020Quote: ... The zona pellucida of individual embryos was removed by brief incubation and pipetting in prewarmed acid Tyrode’s solution (Sigma). Embryos were transferred to a Fluorodish (WPI ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal volumes of lavage samples and a 20 mg/mL sinapic acid matrix solution (Sigma Aldrich, St. Louis, MO) in 30/70 ACN/H2O with 1% TFA were mixed together for a final concentration of 2,500 cells/μL ...
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bioRxiv - Immunology 2020Quote: ... cells were immediately transferred to pre-chilled tubes and washed in cold ddPBS before trichloroacetic acid (TCA, Sigma Aldrich)/acetone (Fisher chemical ...
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bioRxiv - Immunology 2019Quote: ... 7 μm cryosections were stained with Hemalum solution acid (Carl Rorth; T865.1) and Eosin Y aqueous solution (Sigma, 201192A). Human type VII collagen was stained by immunofluorescence using anti-human type VII collagen antibody (anti-NC-1 domain of type VII collagen (LH7.2 ...
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bioRxiv - Bioengineering 2019Quote: ... Synthetic Complete (SC) medium was prepared using 6.7 g/L Yeast Nitrogen Base without amino acids from Sigma-Aldrich, 1.92 g/L Synthetic Drop-out supplement without histidine from Sigma-Aldrich and 76 mg/L histidine ...
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bioRxiv - Developmental Biology 2019Quote: ... To block high fat meal induced EEC silencing, sodium tauroursodeoxycholic acid (TUDCA; 0.5mM, T0266) or orlistat (0.1mM, Sigma O4139) were added to the high fat meal solution and zebrafish were treated for the indicated time.
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bioRxiv - Immunology 2019Quote: ... The experiment was initiated by adding 3 µCi [9,10−3H]-palmitic acid complexed to 5% BSA (lipids free; Sigma) and ...
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bioRxiv - Biochemistry 2019Quote: ... Antibodies were diluted to 17.5 μg/ml in 100 μl 0.1M 2[N-Morpholino]ethanesulfonic acid (MES)-buffer (M2933, Sigma-Aldrich), pH 4.5 ...
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bioRxiv - Immunology 2019Quote: ... Embryos were grown at 28.5°C and kept under anesthesia with egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma) during bacterial injections ...
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bioRxiv - Cell Biology 2019Quote: ... Then 2,5 Mл culture medium (α-MEM, 10% FBS, 100 μM L-ascorbic acid 2-phosphate (Sigma-Aldrich, USA), 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2019Quote: DNA concentration of patient sputum was determined by the reaction of 3,5-diaminobenzoic acid dihydrochloride (DABA; Sigma Aldrich, MO) with DNA aldehydes ...
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bioRxiv - Neuroscience 2019Quote: ... The spinal cords were dissected and fixed in 4% paraformaldehyde (PFA) and 5% saturated picric acid (Sigma-Aldrich, P6744) in phosphate buffer saline (PBS ...
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bioRxiv - Immunology 2019Quote: ... To analysed caecal crypts and goblet cell counts sections were stained with periodic acid Schiff’s reagent (PAS) and counterstained with Meyers haematoxylin(Sigma). To analyse mucin sulphation ...
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bioRxiv - Genetics 2021Quote: ... and resuspended in 250 µL of Qiagen buffer P1 and 200 µL of acid-washed glass beads (0.5 mm; Sigma). Samples were vortexed on high for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... plates were washed 10 times with PBS/Tween and substrate was added (o-phenylenediamine dihydrochloride tablets) and then quenched with 0.2 N sulfuric acid (Sigma) after colour appears visible ...
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bioRxiv - Immunology 2021Quote: ... The digested products were then diluted with formic acid to a final concentration of 0.1% and cleaned up using ZipTip C18 (Merck Millipore). Data was obtained on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Vaginal plugs were assessed and pregnant mice with gestation day (GD) 17 were injected intravenously (i.v.) with 5mg/kg PolyI:C (Polyinosinic-polycytidylic acid; P9582, Sigma, USA). Aliquots of 5mg/ml were prepared by resuspending in the sterile 0.9% saline and were stored at −20°C until further use ...
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bioRxiv - Plant Biology 2020Quote: ... The peptides were dissolved in 2% acetonitrile (v/v) /0.1% formic acid (v/v) and filtered with Ultrafree-MC Centrifugal Filters (PVDF 0.45 μm; Millipore, USA). Peptides were identified using an EASY-nLC 1000 liquid chromatograph coupled to an Orbitrap Mass Spectrometer (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... product formation was quantified using a 0.05 – 22 µM calibration curve of the free EDANS acid (Sigma PN A6517). Product concentrations were adjusted for inner filter absorbance effects with correction factors generated by comparing the fluorescence of 2 µM EDANS in solution with each concentration of substrate used to that with no substrate ...