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Citations for Millipore Sigma :
751 - 800 of 2304 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and cells were overlaid with virus growth medium containing Avicel microcrystalline cellulose (final concentration of 1.2%) (Sigma-Aldrich). The plates were then incubated at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
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bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated in 1.5 mL virus-containing media supplemented with 0.5 mL fresh media and 0.8 μg/mL polybrene (Millipore Sigma). The 6-well plate was covered with aluminum foil to keep cells in the dark ...
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bioRxiv - Microbiology 2022Quote: ... A27(-) and H3(-) virus stocks were generated in the absence of isopropyl β-d-thiogalactopyranoside (IPTG; Sigma 16758). Plaque forming units /ml and particle counts - calculated from the optical density at 260 nm44 - were determined for each purified recombinant VACV MV stock.
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bioRxiv - Cancer Biology 2022Quote: ... filtered (0.45 µm) and CDX cells were infected with 1 mL virus containing 12 µg/mL polybrene (Sigma), followed by selection with Puromycin (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... the virus-containing media was removed and N2 media with doxycycline (DOX, 2 µg/mL, Sigma, catalog #: D9891) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were transduced with lenti-shPGC1α RNA virus with 1:2000 dilution polybrene (Millipore, TR-1003-G) for 24 h and then media was changed ...
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bioRxiv - Pathology 2023Quote: ... Stock virus was produced after three serial passages in Vero E6 cells using Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 2 % fetal bovine serum ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The virus suspension was immediately by passed through a Sephadex G-10 (Sigma-Aldrich, St. Louis, MO, USA) filter column via centrifugation (5 minutes ...
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bioRxiv - Immunology 2023Quote: ... virus-infected or HBSS-injected larvae were exposed to either dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO), DMSO-solubilized ramipril (Cayman Chemical Company ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Virus containing media was added to ATG3 KO HeLa cells with 8 μg/mL polybrene (Sigma-Aldrich, H9268). After a day ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was concentrated to a volume of 0.25 mL in a 100KD cutoff Amicon centrifugal filter (Millipore UFC910096) pretreated with a series of DPBS+ Pluronic F68 (Fisher 24-040-032 ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates incubated with anti-flag M2 beads (Sigma-Aldrich, St. Louis, USA) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then treated with 50U/ml of Benzonase (Novagen #70664), for 30min in a 37°C water bath ...
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bioRxiv - Cell Biology 2019Quote: ... Pre-cleared cell lysates were incubated with anti-FLAG beads (Sigma, A2220) or anti-HA beads (Sigma ...
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bioRxiv - Genetics 2021Quote: ... The adjusted lysates were combined with anti-VSV-G agarose beads (Sigma) that had been washed once with IP buffer and reconstituted to a 50/50 bead/buffer slurry ...
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bioRxiv - Genomics 2020Quote: ... Protein concentration of tissue lysates was determined using bicinchoninic acid (Sigma-Aldrich). Proteins were denatured with gel loading buffer (50 mM Tris [pH 6.8] ...
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bioRxiv - Developmental Biology 2021Quote: ... crude cell lysates were diluted in 2x Laemmli buffer (Sigma-Aldrich, #S3401) and denatured for 5 min at 95°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the lysates were loaded onto Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) and incubated for 2 h at 4°C with overhead shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysates were combined with 20μL of ANTI-FLAG M2 (Sigma-Aldrich) magnetic beads that had been pre-washed 2x with Buffer A then incubated for 2 hr on a rotating wheel at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Filtered lysates were incubated with the anti-FLAG beads (Sigma-Aldrich, M8823) for 2 hrs at room temperature (22 °C) ...
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bioRxiv - Immunology 2021Quote: ... Lysates were resolved on SDS-PAGE and transferred to PVDF membranes (Millipore), followed by immunoblotting as previously described (Haniuda et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... cleared lysates were incubated with Anti-FLAG® M2 Magnetic Beads (SIGMA) for 2h and washed two times in lysis buffer and one time in TBS ...
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bioRxiv - Molecular Biology 2019Quote: ... lysates were incubated with anti-FLAG M2 ® magnetic beads (Sigma M8823). After incubation ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclear lysate was used for immunoprecipitation with Flag M2 Magnetic Beads (Sigma) for 2h at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... whole cell lysates (WCLs) were precleared using protein A Sepharose (Sigma-Aldrich) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Each cell lysate was incubated with ANTI-FLAG M2 affinity gel (Sigma) at 4°C overnight on a shaker at 10 rpm ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were sonicated and 10 % v/v β-mercaptoethanol added (Sigma-Aldrich). Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... Subsequent lysates were supplemented with 10% Protease Inhibitor Cocktail (PrIC; Sigma-Aldrich), 1% Phosphatase Inhibitor Cocktail 2 (PhIC ...
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bioRxiv - Bioengineering 2020Quote: ... The lysate was filtered with Amicon 30MWCO filters (Millipore Sigma, Catalog #UFC8030) according to the manufacturer’s protocol and washed three times to exchange the buffer with the reaction buffer (45mM sodium phosphate ...
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bioRxiv - Bioengineering 2020Quote: ... total protein was collected from cell lysate in RIPA buffer (Sigma, R0278) containing 1% phosphatase inhibitor cocktail 3 (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were then subjected to preclearing with Sepharose 4B beads (Sigma) at 4 °C for 1 h with agitation ...
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bioRxiv - Cell Biology 2021Quote: ... the lysate was incubated with 25 U benzonase HC nuclease (Sigma-Aldrich) at 37 °C for 30 min before IP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were incubated with Anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) for 4 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2021Quote: Whole-cell lysates were prepared with RIPA buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Systems Biology 2021Quote: ... whole-cell lysates were incubated with Concanavalin A-Sepharose 4B (Sigma-Aldrich). Whole-cell lysates were rotated at 4°C for 1 hour with 100 ml of 50% Con-A slurry ...
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bioRxiv - Neuroscience 2020Quote: ... Cleared lysates were incubated with 10 μg of 2A8 antibody (EMD-Millipore) (Nelson et al. ...
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bioRxiv - Microbiology 2021Quote: ... Protein content of the cell lysates was determined using BSA assay (Sigma).
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were subjected to SDS-PAGE and transferred to PVDF membrane (Millipore). Membranes were blocked in skim milk or BSA and probed overnight with the following antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were vortexed 4 x 45 sec with glass beads (Sigma Aldrich), maintained on ice between pulses ...
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bioRxiv - Cell Biology 2021Quote: ... lysates were incubated with anti-M2 FLAG-conjugated agarose beads (Sigma-Aldrich) at 10 ul bed volume per 1 mg of protein and incubated for 1 hour at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was applied to 0.5 mL of glutathione agarose (Sigma-Aldrich) per liter of culture for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Whole-cell lysates were prepared with RIPA buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were then supplemented with 5-10 mM imidazole (Sigma #I5513), added to 1 ml of washed Ni2+-NTA agarose (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was then treated with Benzonase nuclease HC (#71206-3, Millipore) for 45 minutes at 37 oC ...
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bioRxiv - Pathology 2022Quote: ... The lysate was then treated with Benzonase nuclease HC (#71206-3, Millipore) for 45 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Then cell lysate was added to Streptavidin Agarose conjugated beads (Millipore, USA) and incubated for 2 h with gentle rotation ...