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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for Recombinant Mouse Icam2 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). For analysis of neutrophil activation ...
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bioRxiv - Cell Biology 2023Quote: ... was mixed with unconjugated Fc-antibody (5 µg/ml, Sigma-Aldrich, # I2136) in HBSS and incubated for 30-60 minutes at room temperature with gentle agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Hybridoma cells were selected in DMEM/10% FCS containing HAT (Sigma, MO) and 1% BM-Condimed (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich; St. Louise, MO), penicillin streptomycin glutamine (100 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Biochemistry 2024Quote: ... the supernatant supplemented with 1% Pen/Strep and 10% FCS (Sigma-Aldrich) and stored in the dark at 4°C.
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bioRxiv - Bioengineering 2024Quote: ... or horseradish peroxidase-conjugated goat anti-human IgG Fc antibody (Sigma, A0170) diluted 1:20,000 in the blocking buffer for an hour at 37°C ...
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bioRxiv - Biochemistry 2020Quote: 3XFLAG-tagged Nth1 was immunoprecipitated using Anti-FLAG™ M2 magnetic beads (Sigma, product number: M8823). Magnetic beads were washed three times with wash buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Plant Biology 2021Quote: ... Affinity purification of tagged RPL18-containing polysomes was carried out using anti-FLAG beads (Sigma-Aldrich) incubated with the supernatant at 4 °C for 3 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Neuroscience 2022Quote: ... To immunoprecipitate FLAG-tagged proteins the supernatant was incubated with anti-FLAG M2 Affinity Gel (Sigma) for 1h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Sequences were inserted into the Bgl Ⅱ/Acc65I (TOYOBO) digested FLAG-tagged vector pCMV-FLAG4 (Sigma). pCMV-FLAG4 was used as a control.
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: Purified FLAG-tagged CSB proteins were immobilized on pre-equilibrated Anti-FLAG M2 Magnetic Beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Genetics 2020Quote: ... Epitope-tagged proteins were immunoprecipitated with either anti-Flag- or anti-HA-coated magnetic beads (Sigma and Pierce ...
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bioRxiv - Biophysics 2020Quote: ... pH 7.4 for affinity purification of the FLAG-tagged cHMM on M2 mAb-Sepharose beads (Sigma). Bound cHMM was eluted with 0.1 mg⋅mL−1 FLAG peptide (Sigma) ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged Srb10/ Cdk8 was recovered by immunoprecipitation with anti-FLAG-conjugated M2 agarose beads (Sigma). Samples were washed two times in KLB followed by two times in kinase assay buffer (KAB ...
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bioRxiv - Biochemistry 2022Quote: ... The strep-tagged complex was eluted from the resin using buffer containing 5 mM desthiobiotin (Sigma), followed by SEC.
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Microbiology 2023Quote: ... and FLAG-tagged UL31 plasmid constructs using of X-tremeGENE HP DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and FLAG-tagged UL31 plasmid constructs using of X-tremeGENE HP DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was carried out in freshly made blocking buffer (5% FCS, Sigma-Aldrich, Gillingham ...
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bioRxiv - Immunology 2022Quote: ... in 10 ml VLE DMEM (Biochrom) containing 10% heat-inactivated FCS (Sigma-Aldrich), 0.1% 2-mercaptoethanol (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Spleens were gently meshed in FACs buffer (PBS containing 3% FCS from Sigma) using a 40μM cell strainer to prepare single cell suspensions ...
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bioRxiv - Molecular Biology 2019Quote: ... MDCKII cells were grown in DMEM + 10%FCS + Antibiotic/Antimycotic solution (Sigma A5955)(Williams et al. ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 10% FCS tested to be dox-free (Sigma-Aldrich, Taufkirchen, Germany) and penicillin and streptomycin (final concentrations 100 units/ml and 100µg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in which the plate was coated with anti–human Fc (Sigma, Catalog # FAB3700259), and after overnight incubation at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the media was replaced by FCS-free medium containing 0.1% BSA (Sigma-Aldrich) and cells were stimulated with 100 μl milk EVs or EV-depleted controls in the presence or absence of 5 μg/ml Poly I:C (Invivogen) ...
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bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO, USA), 10mM HEPES ...
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bioRxiv - Synthetic Biology 2020Quote: ... cell media was supplemented with 500 μg/mL 5-FC (Sigma, MO, USA). For control conditions ...
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bioRxiv - Immunology 2019Quote: ... before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich], 90% FCS [Sigma-Aldrich]). This method preserves immune cell viability ...
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bioRxiv - Immunology 2019Quote: ... before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich], 90% FCS [Sigma-Aldrich]). This method preserves immune cell viability ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
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bioRxiv - Plant Biology 2020Quote: ... using either 0.5 μg of recombinant histone H3 (Millipore, Cat. #14-411), 2 μg of chicken core histones (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Cell Biology 2020Quote: ... 14-3-3 antibody and active recombinant AMPK (α2β1γ1) were from Millipore. Precast Tris-Glycine polyacrylamide gels and 10X tris-glycine gel electrophoresis buffer were from BioRad ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Genetics 2020Quote: ... 0.5 U DNA recombinant Taq polymerase in buffer (pH= 8.0; Sigma, Germany), 1x PCR buffer (pH=8.7 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 ng/mL of recombinant basic fibroblast growth factor (Sigma-Aldrich). RAW 264.7 macrophages were cultured in complete media composed of high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM (1x ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...