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Citations for Millipore Sigma :
751 - 800 of 4062 citations for Recombinant Human OXSR1 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The primary immunization was carried out using 450 μg of GST cleaved PfCD protein and an equal volume of Freund’s complete adjuvant (F5881 # Sigma-Aldrich) via the intraperitoneal route ...
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bioRxiv - Immunology 2020Quote: ... 1mM EDTA, and protease inhibitors (aprotinin, leupeptin, PMSF) GST-Hhex fusion proteins were purified over glutathione-Sepharose 4B beads (Sigma) and eluted with 10 mM glutathione according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: A peptide corresponding to residues 1 – 159 of HyYap was expressed as GST-fusions using the GEX4t-1 vector (Millipore) in E ...
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bioRxiv - Developmental Biology 2019Quote: Peptides corresponding to the C-terminal portions of HyFat (residues 4191 – 4393) and HyDs (residues 9144 - 9799) were expressed as GST-fusions using the GEX4t-1 vector (Millipore) in E ...
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bioRxiv - Cell Biology 2019Quote: ... After washing 3–4 X with 1× PBS-T the strips were incubated with 1:5000 mouse anti-GST antibodies (Sigma). The secondary antibodies (anti-rabbit-HRP or anti-mouse-HRP ...
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bioRxiv - Cancer Biology 2019Quote: ... Activated p38MAPKα was added to a final concentration of 60 pM for reactions with 1nM non-phosphorylated GST-MK2 (Millipore Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... phosphorylation of peptides by the active Pro-directed kinase GST-CDK2/Cyclin A2 (100 ng purified from Sf9 cells, Sigma) was also confirmed in the presence or absence of the ATP competitive inhibitor ...
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bioRxiv - Systems Biology 2020Quote: ... the cDNA was inserted in frame with a 6xHis-GST TEV-cleavable tag into a modified version of pET-30b (Novagen). The NCK2 SH3 shuffled chimeras were constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant GST fusion proteins were eluted by resuspending the resin in elution buffer containing 15 mg/mL reduced L-glutathione (Sigma) and incubated at 4°C for 4 hours ...
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bioRxiv - Plant Biology 2022Quote: ... A 1–month–old ICR mouse (SLAC laboratory animal) was injected with 50 μg GST–CAR9 protein emulsified with Complete Freund’s adjuvant (F5881, Sigma–Aldrich). Two weeks later ...
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bioRxiv - Plant Biology 2021Quote: ... GSTU5 was detected by the anti-GST primary antibody (Novex, 1:500) and anti-mouse secondary antibody (Sigma, 1:5000).
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bioRxiv - Cell Biology 2019Quote: ... Elution was done by incubation of the beads in 30µl GST elution buffer [50 mM Tris-HCl (pH 8.0) and 20mM reduced glutathione (Sigma-Aldrich, #G4251)] at room temperature for 20mins ...
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bioRxiv - Biochemistry 2021Quote: ... FH-nsp13 and GST-nsp13 were affinity purified from insect cell lysates using anti-FLAG M2 Affinity Gel (Sigma-Aldrich) or Glutathione Sepharose 4 Fast Flow resin (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: A Rab11/Ypt32 construct harbouring a GST tag at the N-terminus was expressed recombinantly in Rosetta2 cells (EMD Millipore) and purified using the procedure described previously (21) ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-Cter-α4 or GST-β3) was mixed with 1.5 μL of active Ccnd1-Cdk4 complex purified from baculovirus (Sigma C0620), 10 mM ATP ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant insulin human (≥ 98%), somatostatin-14 (≥ 97%, human HPLC grade), and urocortin-3 (≥ 97%, human HPLC grade) were all purchased from Sigma-Aldrich. Taxonomy will only be indicated for insulin ...
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bioRxiv - Biochemistry 2020Quote: 3XFLAG-tagged Nth1 was immunoprecipitated using Anti-FLAG™ M2 magnetic beads (Sigma, product number: M8823). Magnetic beads were washed three times with wash buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Plant Biology 2021Quote: ... Affinity purification of tagged RPL18-containing polysomes was carried out using anti-FLAG beads (Sigma-Aldrich) incubated with the supernatant at 4 °C for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 constructs were visualised with primary antibody mouse α-FLAG (F1804; 1/200; Sigma) for 2 hours at 37°C in a humidified chamber ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Neuroscience 2022Quote: ... To immunoprecipitate FLAG-tagged proteins the supernatant was incubated with anti-FLAG M2 Affinity Gel (Sigma) for 1h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Sequences were inserted into the Bgl Ⅱ/Acc65I (TOYOBO) digested FLAG-tagged vector pCMV-FLAG4 (Sigma). pCMV-FLAG4 was used as a control.
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: Purified FLAG-tagged CSB proteins were immobilized on pre-equilibrated Anti-FLAG M2 Magnetic Beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Genetics 2020Quote: ... Epitope-tagged proteins were immunoprecipitated with either anti-Flag- or anti-HA-coated magnetic beads (Sigma and Pierce ...
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bioRxiv - Biophysics 2020Quote: ... pH 7.4 for affinity purification of the FLAG-tagged cHMM on M2 mAb-Sepharose beads (Sigma). Bound cHMM was eluted with 0.1 mg⋅mL−1 FLAG peptide (Sigma) ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged Srb10/ Cdk8 was recovered by immunoprecipitation with anti-FLAG-conjugated M2 agarose beads (Sigma). Samples were washed two times in KLB followed by two times in kinase assay buffer (KAB ...
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bioRxiv - Biochemistry 2022Quote: ... The strep-tagged complex was eluted from the resin using buffer containing 5 mM desthiobiotin (Sigma), followed by SEC.
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Microbiology 2023Quote: ... and FLAG-tagged UL31 plasmid constructs using of X-tremeGENE HP DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and FLAG-tagged UL31 plasmid constructs using of X-tremeGENE HP DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Cancer Biology 2024Quote: ... and equilibrated before FLAG tagged proteins were immunoprecipitated using anti-Flag M2 agarose beads (Sigma #A2220).
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Plant Biology 2020Quote: ... using either 0.5 μg of recombinant histone H3 (Millipore, Cat. #14-411), 2 μg of chicken core histones (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Cell Biology 2020Quote: ... 14-3-3 antibody and active recombinant AMPK (α2β1γ1) were from Millipore. Precast Tris-Glycine polyacrylamide gels and 10X tris-glycine gel electrophoresis buffer were from BioRad ...
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bioRxiv - Genetics 2020Quote: ... 0.5 U DNA recombinant Taq polymerase in buffer (pH= 8.0; Sigma, Germany), 1x PCR buffer (pH=8.7 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 ng/mL of recombinant basic fibroblast growth factor (Sigma-Aldrich). RAW 264.7 macrophages were cultured in complete media composed of high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM (1x ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Bovine erythrocyte ubiquitin and recombinant hsNEDD8 were purchased from Sigma-Aldrich (U6253) and BostonBiochem (UL-812 ...
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bioRxiv - Microbiology 2020Quote: ... A set of recombinant RT standards (EMD Millipore, Molsheim, France; catalog# 382129) ranging from 5.12e3 nU/mL to 5.12e9 nU/mL was used to estimate the nU/mL of reverse transcriptase activity in the FIV stock.