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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... mouse recombinant IFN-β (Millipore) diluted in DPBS with 0.1% Bovine serum albumin BSA (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Genetics 2019Quote: ... 15 U recombinant DNase1 (Sigma) and 10 U DNA polymerase I (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant DNAse I (Sigma) in MACS buffer (PBS/2mM EDTA/ 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
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bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
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bioRxiv - Microbiology 2021Quote: Recombinant mouse adiponectin (Sigma, #SRP3297) and GFP protein (Sino Biological ...
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bioRxiv - Physiology 2022Quote: Lyophilised recombinant Aβ42 (#AG912, Millipore) and scrambled control peptide (ScrAβ42 ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: Recombinant Mpro (Sigma Aldrich, SAE0172) was resuspended in 10% aqueous glycerol to a concentration of 1 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse anti-T7 primary antibody (Millipore Sigma, 69522) was applied at a dilution of 1:1000 for 2 hrs at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... After Immunoprecipitations using T7 agarose (69026, MERCK Millipore), Anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Cancer Biology 2019Quote: ... T7-Tag (Catalog Number 69522) from EMD Millipore, or β-Actin clone AC-15 (Catalog Number A5441 ...
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bioRxiv - Genomics 2022Quote: ... in a 12-well cell culture plate coated with Human recombinant laminin 511 (BioLamina, Cat. LN511-0202) and fixed in 1xPBS with 3.7% formaldehyde (Millipore Sigma, Cat. F8775-25ML) for 10 mins and then permeabilized in ice-cold 70% (vol./vol. ...
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bioRxiv - Cell Biology 2023Quote: ... East Brunswick, NJ, USA), human recombinant Stem Cell Factor (100ng/mL, supernatant SCF producing cell line) and dexamethasone (1μM; Sigma, St. Louis, MO, USA).5 EBL cultures were kept at a density of 0.7-1.5 million/mL by dilution with fresh medium ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Epidemiology 2019Quote: ... and recombinant heat labile toxin β subunit protein [15,52,53] from enterotoxigenic Escherichia coli (ETEC LT β subunit) were purchased from Sigma Chemical (St ...
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bioRxiv - Bioengineering 2021Quote: ... Columns were washed with 20 column volumes PBS and recombinant proteins were eluted with 100 mM β-d-lactose (Sigma) prepared in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were overexpressed in E.coli BL21 (DE3) at 17 °C overnight and then purified by Glutathione-agarose (Sigma #G4510). Briefly ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Biochemistry 2022Quote: ... and fractions judged by SDS-PAGE to contain the recombinant protein were pooled and desalted by size exclusion chromatography using 30K centrifugal spin column (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... For capture of antibodies and Fc-Fusions a CM5-S-Series sensor chip (Cytiva) was functionalized with 2500RU recombinant Protein A (Sigma) using EDC/NHS Coupling Kit (Biacore/Cytiva BR-1000-50 ...
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bioRxiv - Plant Biology 2019Quote: ... using a VP-ITC microcalorimeter (MicroCal) as described [34] after extensive dialysis of the purified recombinant protein in Chelex 100 (Sigma) to remove contaminating divalent cations ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Cell Biology 2019Quote: ... Immune sera were generated by immunization of 6 weeks-old female NMRI mice (Charles Liver Laboratories) via subcutaneous injection of 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma-Aldrich) followed by a boost after 4 weeks with 50 µg of recombinant protein ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biophysics 2021Quote: Catalytic activity was evaluated in vitro using 20 pmol of purified recombinant proteins in a 200 µl reaction buffer supplemented with 20 mM p-nitrophenyl phosphate (Sigma) as substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Immunology 2021Quote: ... and cloned into the mid-copy T7-FNS2 phage display vector as described previously,17 and packaged using the T7 Select Packaging Kit (EMD Millipore). Library quality assessment was performed as described in Results.
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bioRxiv - Cell Biology 2022Quote: ... and dextran tagged with fluorescein (40,000 MW, Sigma Aldrich) were resuspended in 100 μl HBSS (4 mg/ml ...
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bioRxiv - Developmental Biology 2019Quote: Biotin-tagged OSTN was labeled by Cy3-streptavidin (Sigma). Equal volume of 25 μM biotin-tagged OSTN and 0.1 mg/ml Cy3-streptavidin were incubated with on ice for 15 minutes at 4 C ...
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bioRxiv - Cell Biology 2021Quote: ... the TEV-eluted samples were collected and incubated with 20 μL of Anti-Flag agarose beads (Sigma) for 2 hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The TEV cleaved gB was eluted from the beads with TBS pH7.4 containing 1mg/ml lauroylsarcosine (Sigma) and 1mg/ml Amphipol 8-35 (Anatrace) ...
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bioRxiv - Neuroscience 2019Quote: Protein lysates were collected from regional human astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...