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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Virus was then removed and cells were overlayed with 3 mL of DMEM with 0.8% methylcellulose (Sigma), 1% FBS ...
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bioRxiv - Physiology 2020Quote: Virus carrying the gene of interest was used to infect cell lines with 10mg/mL polybrene (Millipore). Media was replaced after 24 h and cells were sorted for GFP positivity after 48-72 h post-infection ...
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bioRxiv - Genetics 2019Quote: ... The resulting myoblasts were then infected with a retroviral cocktail containing Myf6-CTAP virus and polybrene (Millipore) for 8 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Suspensions of L-cells (from ATCC) for virus production were maintained in Joklik MEM medium (Sigma-Aldrich) supplemented with 1% L-Glutamine (Gibco.) ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant containing the virus was collected after 72 hours and filtered through a 0.22 micron PES (Millipore) filter ...
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bioRxiv - Genomics 2021Quote: ... Harvested media containing the desired virus were filtered through Millex-HV 0.45-µm PVDF filters (Millipore, SLHV033RS) and further concentrated with 100,000 NMWL Amicon Ultra-15 centrifugal filter units (Amicon ...
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bioRxiv - Cell Biology 2021Quote: ... supernatant containing propagated virus was filtered through an Amicon Ultra 15 (100 kDa) centrifugal filter (Millipore Sigma) at ~4000 rpm for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... virus was purified using centrifugal filter devices (Centricon Plus-70 and Biomax 100.000, Millipore Corp., Bedford, MA) and stocks with a titre between 3×109 and 3×1010 viral genome equivalents (vge ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa or U2OS cells were transduced with virus for 48 h with 10 μg/ml polybrene (Sigma), then optimized for protein expression via fluorescence sorting or puromycin selection.
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus containing media were harvested 48-64 h later and filtered by 0.45 μm Steriflip filter (Millipore). HepG2 and Huh7 cells were infected with 1 ml virus containing medium with 8 μg/ml polybrene for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was replaced with virus-containing medium supplemented with 8 µg/mL of polybrene (Sigma-Aldrich). Approximately 24 hours post-viral treatment ...
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bioRxiv - Immunology 2022Quote: ... The supernatant containing virus particles was collected after 48h and filtered via 0.45 μm filter (Roth/Millipore). Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15-Cas9 cells were infected with pooled CRISPR library virus using polybrene (8 µg/ml, Sigma) using a low multiplicity of infection (MOI = 0.75 ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Microbiology 2023Quote: ... RNA from the supernatant of cell culture infected with the virus was isolated using the Trizol (Sigma) method described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... NF1-MET cells were infected with 500 μL of 0.45 μm filtered virus with polybrene (EMD Millipore), and 48 hours post infection transduced cells were selected for by 2 μg/mL puromycin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 100 TCID50 of the virus in Intesticult media supplemented with 500 μM sodium glycochenodeoxycholate (GCDCA; Sigma, G0759) was added to 5-day differentiated HIE monolayers in triplicate on a 96-well plate ...
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bioRxiv - Microbiology 2020Quote: ... Human serum-supplemented medium was made through addition of complete human serum (Sigma Aldrich) into CDM to a final concentration of 5% (v/v) ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in an equal volume of human serum from human male AB plasma (Sigma) and incubated for 16 h at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10 % heat-inactivated human serum from human male AB plasma (Sigma-Aldrich), 2.5 μM zoledronic acid (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for immunostaining: anti-CREST/anti-centromere antibodies (human, Antibodies Inc.; 1:100) anti-Tubulin (mouse monoclonal, Sigma; 1:5000). DNA was stained with 0.5 µg/ml DAPI (Serva ...
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Microbiology 2020Quote: ... Freshly washed erythrocytes were diluted to 1% hematocrit in 1X PBS containing 1% BSA and coincubated for an hour with antibodies against the human glycophorin AB (GYPAB) (1:400, Sigma Aldrich UK), GYPA (Clone E4 ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were washed five times and incubated for 1 h with 1 µg/mL HRP-conjugated goat anti- human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with CR3022 anti-S antibody (1 ug/mL)128 overnight followed by anti-human IgG-HRP-conjugated goat secondary antibody (Sigma, 1:500) for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... and human chorionic gonadotropin (Sigma), 48 hours apart ...
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bioRxiv - Biochemistry 2020Quote: ... human cathepsin S (Millipore Sigma), bovine spleen cathepsin C (Millipore Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... 20nM human insulin (Sigma-Aldrich), 2 μM rosiglitazone (Cayman) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% human serum (Sigma-Aldrich), 1x MEM Vitamins (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% human serum (Sigma-Aldrich) and 100 μg/ml primocin (Invivogen) ...
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bioRxiv - Cell Biology 2022Quote: ... 5% human serum AB (Sigma), penicillin-streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: ... Human CTSD ELISA (Millipore #1317) was performed on the culture media according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... and human IFN-γ (Millipore) were used for stimulation of cells ...
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bioRxiv - Bioengineering 2020Quote: ... or human plasma FN (Sigma) solutions ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... and human Fn (Sigma-Aldrich) as described previously (Bao et al. ...
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bioRxiv - Immunology 2021Quote: ... A human IgG (Sigma #I2511) or IgM (Sigma #I8260-1MG ...
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bioRxiv - Bioengineering 2021Quote: ... Human serum albumin (Sigma, USA) was used as a control ...
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bioRxiv - Bioengineering 2021Quote: ... human insulin (Sigma-Aldrich, I2643), single strand DNA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... hemoglobin (human, Millipore Sigma #G4386), gamma-globulin (human blood ...
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bioRxiv - Systems Biology 2021Quote: ... 0.02% human-EGF (Sigma, E5036), and 0.01% cholera toxin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... human plasma-derived apoE (Sigma), or DMSO (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... against human Sept9 (Sigma-Aldrich, Cat#HPA042564 and HPA050627 ...
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bioRxiv - Cell Biology 2021Quote: ... C5 deficient serum human (Sigma); Phalloidin-Alexa 488 & 647 (Molecular Probes) ...
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bioRxiv - Cell Biology 2021Quote: ... in human serum (P2918, Sigma) for 30 min at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... 20 nM human insulin (Sigma), 2 μM Rosiglitazone ...
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bioRxiv - Bioengineering 2022Quote: ... human insulin (Sigma- Aldrich, I2643), single strand DNA (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... 10% human serum (Sigma-Aldrich), 10% goat serum (Jackson Immuno) ...