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Citations for Millipore Sigma :
751 - 800 of 3379 citations for Beta NGF Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Protein from gels were transferred onto PVDF membranes and probed with NSF (SYSY, mouse monoclonal) and beta actin (Sigma, mouse monoclonal) antibodies overnight with shaking ...
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bioRxiv - Physiology 2021Quote: Recipient mice were rendered diabetic before islet transplantation with a single intravenous injection of the beta cell toxin streptozotocin (STZ 200 mg/Kg; Sigma, MO) dissolved fresh in 100 mM sodium citrate (pH 4.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The beta subunit of complex V and proteins bound to it were immunoprecipitated using an antibody to the beta subunit of ATP synthase (Millipore, MAB3494) in accordance with the Pierce™ Co-Immunoprecipitation Kit (Cat.No:26149) ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibody staining was carried out by placing coverslips upside down onto a drop of primary antibody staining mix (mouse-anti-beta-tubulin, catalog no. T5293, Sigma-Aldrich, diluted 1:300 in PBS containing 2% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... 810-2 was dissolved in a vehicle (VEH) solution of 20% hydroxy propyl beta cyclodextrin (HPβCD; Sigma, St. Louis, MO, USA) in acetate buffer [0.1 M acetic acid (from glacial acetic acid stock ...
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bioRxiv - Physiology 2021Quote: The BHB concentration was quantified using the Beta-Hydroxybutyrate Assay Kit (MAK041) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of BHB was expressed in ng/μg protein.
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bioRxiv - Microbiology 2019Quote: ... Primers (Table S1) used for PCR amplification of the beta-lactams genes (blaTEM, blaSHV, blaCXT-M and blaOXA) were acquired from Sigma Aldrich, USA ...
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bioRxiv - Cell Biology 2020Quote: ... Intraperitoneal glucose tolerance test was performed by fasting overnight and injecting 2 g/kg D-glucose solution at 2 weeks after mouse beta cells were ablated with one high dose (150 mg/kg) of streptozotocin (Cat. No. S0130-1G, Sigma-Aldrich). Blood was collected in heparin-coated tubes at fed state ...
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bioRxiv - Neuroscience 2021Quote: ... The biochemical assay of GCase activity was carried out in a buffer containing 4-methylumbelliferyl beta-d-glucopyranoside substrate (Cat. M3633, Sigma Aldrich); after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU ...
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bioRxiv - Plant Biology 2023Quote: ... except that 3 µl beta-mercaptoethanol per sample replaced 1,4-dithiothreitol (DTT) and 0.5% polyvinylpyrrolidone (PVP, mw 360 000, Sigma Aldrich, Steinheim, Germany) was added to the extraction buffer ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes and suspended in medium (DMEM 85% v/v, Corning; FBS 15% v/v, VWR; 1x Penicillin-Streptomycin, VWR; 50 µM beta-mercaptoethanol, Sigma-Aldrich). 10,000 viable cells were plated per well in white polystyrene 384-well microplates (Greiner Bio-One ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were electrophoresed on polyacrylamide gels transferred onto PVDF membranes and probed using the following antibodies: mouse anti-beta-Actin (Sigma #A1978), mouse anti-Vinculin (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Human EndoC-βH1 and rat INS1E beta cells were cultured as previously described [45] and incubated with lentiviruses expressing an NNAT-targeting shRNA (Sigma-Aldrich) or Silencer Select siRNAs (Ambion) ...
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bioRxiv - Microbiology 2023Quote: ... about 50 μg proteins from each sample were reduced with 10 mM tris (2-carboxyethyl) phosphine (TCEP, Adamas-beta) and then alkylated with 40 mM iodoacetamide (IAA, Sigma-Aldrich). Proteins were pre-digested with 0.5 μg trypsin (Hualishi Tech ...
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bioRxiv - Developmental Biology 2023Quote: ... On addition of Osteogenic media (DMEM + Primocin + 10% FBS + 50 µM ascorbic acid + 10 nM dexamethasone + 10 mM beta-glycerophosphate disodium (Sigma, G9422), the cells form mineralized nodules indicating their osteogenic ability as seen in Supplementary Figure 1.
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bioRxiv - Neuroscience 2024Quote: ... For staining the following primary antibodies were used: For labeling of nerve fibers mouse anti-beta-Tubulin III (1:200, Sigma, T8578); for pre-synaptic terminal staining rabbit anti-Synapsin ...
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bioRxiv - Bioengineering 2022Quote: Human ovarian cancer A2780 and Adriamycin resistant human ovarian cancer A2780-R cells were procured from Sigma Inc ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Genetics 2022Quote: ... Human Erythroleukemia (HEL) and human TF-1 cells were cultured in RPMI-1640 medium (Sigma Aldrich #R8758) supplemented with 10% Fetal Bovine Serum (Sigma Aldrich #F0926 ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte- human fibrinogen mixture (Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte-human fibrinogen mixture (Sigma-Aldrich) and 2µL of the mixture is carefully pipetted into the microwell ...
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bioRxiv - Cancer Biology 2021Quote: ... CLK2 (1:1000, sigma, HPA055366-100UL), phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50. Beta-Tubulin (1:5000, Sigma Aldrich, #T7816) and Beta-Actin (1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... After 24 hours cells were treated with 100 μM 5,6-dichloro-1-bold beta-D-ribofuranosylbenzimidazole (DRB) (287891, Sigma St. Louis, MO) for 3 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and Zbtb16 (1:250, Santa-Cruz, Mississauga, ON) Beta-actin was used as a reference protein (1:5000, Sigma-Aldrich, Oakville, ON). Blots were scanned by Amersham Imager 600 imaging system (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: The following primary antibodies were used in the study: mouse anti-tubulin beta III isoform (Tuj1),clone TU20 (MAB1637, Millipore, 1:1000); rabbit anti- β3-Tubulin ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated overnight at 4 °C with anti-hACE2 antibody (1:1000; R&D AF933) and anti-beta actin (1:2000; CST 4970) in 5% BSA (Sigma-Aldrich A9647) in TBST ...
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bioRxiv - Immunology 2022Quote: ... penicillin-streptomycin (Hyclone, Chicago, IL), 10% heat-inactivated fetal bovine serum (Hyclone, Chicago, IL) and beta-mercaptoethanol (Sigma-Aldrich, St. Louis, MO). Cell lines have been recently authenticated and were periodically tested for mycoplasma species and were confirmed negative.
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bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
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bioRxiv - Neuroscience 2023Quote: ... equivalent to about 5 mg/kg/day) was prepared in vehicle made of 0.45% (2-hydroxypropyl)-beta-cyclodextrin (β-CD; both from Sigma-Aldrich, France), and administered in drinking water for 28 days ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA/DNA and alpha/beta tubulin were tested using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 µg of PfCoSP was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... a second set of tissue from each rat was incubated in a cocktail of rabbit anti-cFos (1:1000) and a mouse monoclonal antiserum against dopamine beta-hydroxylase (DbH; 1:5000; Millipore, MAB308; AB_2245740), followed by a cocktail of Cy3-conjugated donkey anti-rabbit IgG (red ...
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bioRxiv - Genetics 2023Quote: Tissue lysates in RIPA buffer containing protease and phosphatase inhibitors as above were used for ELISA measurements of IL-1 beta (#RAB0275, Sigma Aldrich, GmbH) and IL-6 (#RAB0309 ...
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bioRxiv - Cell Biology 2023Quote: ... Microtubules were linked to PEG-silanized coverslips by incubating chambers with 33 μg/mL monoclonal anti-beta-tubulin III (neuronal) antibody (Sigma-Aldrich, T8578) for 5 minutes prior to washing with 10 mg/mL BSA for 2 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized human haptoglobin phenotype 1-1 (Hp) and human α,β haemoglobin (Hb) were purchased from Sigma Aldrich. Deglycosylation enzyme PNgase F was from New England BioLabs Inc ...
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bioRxiv - Biophysics 2021Quote: PAI-1 (human recombinant, Sigma-Aldrich, Germany)
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bioRxiv - Biophysics 2021Quote: Fibrinogen (human plasma purified protein, Sigma-Aldrich)
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bioRxiv - Biophysics 2021Quote: ... PAI-1 (human recombinant, Sigma-Aldrich, Germany), Plasminogen (human plasma purified protein ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human thrombin (1 U/ml, Sigma) was added to the bottom of a 3.0 µm costar polycarbonate transwell membrane insert (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... sativa–derived recombinant human albumin (Sigma-Aldrich), and 213μg/ml L-ascorbic acid 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 10μM recombinant human Gastrin (Sigma-Aldrich G9145), 50ng/mL recombinant human HGF (Peprotech 100-39H) ...
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bioRxiv - Cell Biology 2019Quote: Primary human osteoblasts were purchased from Sigma. Co-culture cells were titrated to determine the highest viable seeding density to allow continuous culture for the duration of the osteoclast differentiation assay ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 IU human chorionic gonadotropin (hCG, Sigma) was injected 76 h later (14:00 on day 4 of estrous cycle ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 nM human gastrin I (Sigma-Aldrich), 500 nM A 83-01 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... coated with human plasma fibronectin (Millipore Sigma) diluted to 100 μg/mL in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Human B-lymphoid (Sigma-Aldrich, Buchs Switzerland), 293T Flp-in T-REX (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... 10% human serum (Sigma, St. Louis, MO), 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Developmental Biology 2020Quote: ... and human chorionic gonadotropin (hCG) (Sigma Aldrich). Fertilized oocytes were then transferred into M2 medium (Millipore ...
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bioRxiv - Bioengineering 2019Quote: ... human tenascin-C (Millipore Sigma, Catalog #CC065), and human plasma fibronectin (Millipore Sigma ...