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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Moreover, a non-targeting control siRNA (NT siRNA, or siCON) with the sequence 5’-CGUACUGCUUGCGAUACGGUU-3’ was used (Sigma-Aldrich). Cells were transfected with 22 pmol of siRNA oligonucleotides/well using Lipofectamine RNAiMAX (ThermoFisher Scientifc ...
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bioRxiv - Bioengineering 2024Quote: ... silicone plates were plasma treated for 5 minutes followed by incubation with 10% (3-aminopropyl)triethoxysilane (APTES; #440140; Sigma-Aldrich) in ethanol at 60°C for 60 minutes ...
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bioRxiv - Biophysics 2024Quote: ... labelled BGZP-C were prepared in 25 mM 2H-glycine buffer at pH 11.0 or 25 mM phosphate buffer at pH 6.0 in either straight 3 mm tubes (Wilmad, Vineland, NJ) or 5 mm susceptibility-matched microcells (Shigemi, Sigma-Aldrich). Separate samples ...
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bioRxiv - Biophysics 2024Quote: ... The reaction was stirred under N2 for 5 minutes before 0.71 mg (1.05 eq) of 3-morpholinopropylamine (Sigma-Aldrich Inc) was added ...
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bioRxiv - Microbiology 2024Quote: ... and their lungs were minced with scissors and digested in 3 ml of RPMI-1640 with 5% heat-inactivated FBS containing 1 mg/ml collagenase D (Sigma) and 30 µg/ml DNAseI (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 minutes and washed 3-4 times in PBS before being mounted in Mowiol mounting media (Millipore, 475904-M).
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bioRxiv - Microbiology 2024Quote: ... mice were intranasally administered with 3 µg of the RSP protein and 5 μg of cholera toxin (CT; Sigma-Aldrich) as immunological adjuvant ...
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bioRxiv - Bioengineering 2024Quote: ... or 3-5×105 pelleted cells were lysed in TRI Reagent (“LS” for fluid EV samples, conventional for cells, Merck Millipore). Liquid chloroform was added to the lysates at a 1:5 v/v ratio and vigorously mixed for 30 s ...
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bioRxiv - Pathology 2024Quote: ... infiltrated leaves were treated with PBS buffer containing 2% dimethyl sulfoxide (DMSO; control) or an equal volume of DMSO with 5 mM 3-MA (Sigma) for inhibition of autophagy ...
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bioRxiv - Biochemistry 2024Quote: ... and 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (PI(4,5)P2) in chloroform: methanol (Millipore MX0488): water (20:9:1 ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of cell suspension was mixed with 5 µL of fibrinogen solution (6 mg/mL, final concentration 3 mg/mL, 8630, Sigma) by pipetting up and down three times ...
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bioRxiv - Biochemistry 2024Quote: RNA oligonucleotides derived from MALAT1 (5’-AACUU AAUGU UUUUG CAUUG GACUU UGAGUU -3’) were synthesized with or without m6A modification (Sigma). Oligonucleotides and the indicated pri-miRs were radiolabeled at 5’ ends with γ32P-ATP using T4 polynucleotide kinase ...
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bioRxiv - Developmental Biology 2024Quote: ... and then stained for 2 minutes at room temperature using filtered 0.5% Solvent Black 3 (CAS Number 4197-25-5; Sigma 199664) dissolved in 75% ethanol ...
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bioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) in de-ionised (DI ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) as counterstain.
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bioRxiv - Molecular Biology 2021Quote: ... 4% 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS, Sigma)] ...
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bioRxiv - Neuroscience 2022Quote: ... 3 3’-diaminobenzidine tetrahydrochloride hydrate (DAB) (Sigma SIG-32750) in sodium acetate ...
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bioRxiv - Bioengineering 2021Quote: ... 3- [(3- Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS, Sigma RES1300C), digitonin (Sigma D141) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 3-3’ diaminobenzidine tetrahydrochloride (DAB, Sigma Aldrich) staining ...
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bioRxiv - Microbiology 2022Quote: ... 3-amino-9-ethylcarbazol (3-AEC; Sigma Aldrich, Germany) was used as a chromogen ...
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bioRxiv - Microbiology 2021Quote: ... 3-Amino-1,2,4-triazole (3-AT, A8056, Sigma Aldrich). Bovine liver catalase (C1345 ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µM CHIR99021/GSK-3 Inhibitor (Millipore Sigma 361571), and 1 µM PD0325901/MEK1/2 Inhibitor (Millipore Sigma 444968 ...
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bioRxiv - Microbiology 2021Quote: ... 3-(3-hydroxy-phenyl)propionate (Sigma Cat Number PH011597) and 3-hydroxycinnamic acid (CAS Number 14755-02-3) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% 3-3′ diaminobenzidine tetrahydrochloride hydrate (DAB, Sigma D5637) and 4% sucrose in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Interleukin-3 (IL-3, Cat# SRP4135-10UG, Sigma, USA), Alexa Fluor 488 EGF complex (Cat# E13345 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 0.5 nM neurotrophin-3 (NT-3, Sigma #SRP6007). Cells were released by trituration through fire-polished glass pipettes of decreasing diameter ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/ml 3′,3′-diaminobenzidine tetrahydrochloride hydrate (Sigma), 24 units/ml catalase from bovine liver (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... and activating transcription factor 3 (ATF-3, HPA001562, Millipore). After three washes with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 mM 3-methoxybenzylamine (3-MBZ, Sigma-Aldrich #159891), and 0.5 mM benzylamine (BZA ...
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bioRxiv - Biochemistry 2020Quote: Gene expression of endogenous CENP-C was inhibited using a single siRNA (target sequence: 5’-GGAUCAUCUCAGAAUAGAA-3’ obtained from Sigma-Aldrich), which targets the coding region of CENP-C mRNA ...
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bioRxiv - Cell Biology 2020Quote: ... Inserts were then washed 3 times for 5 min in 0.3% TritonX (Simga; T8787) in PBS and blocked for 1 hr at RT in 5% normal donkey serum (NDS, Millipore; S30), 1% BSA 0.3% TritonX in PBS ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies at appropriate dilutions (Supplementary Table 3) were prepared in blocking buffer with 0.1% Tween 20 (Sigma, 9005-64-5). The membrane was incubated in the primary antibody solution overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µL of cells were transferred into 100 µL of staining solution (Phosphate Buffer Saline + 3 µM propidium iodide (Sigma-Aldrich) + 200 nM YP (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... by plating them on NB agar medium (2% glucose, Ade, Ura) supplemented with different concentrations (5-50mM) of 3-aminotriazole (3AT; Sigma-Aldrich) histidine inhibitor ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized oocytes were collected from 3-4 week-old superovulated C57Bl6 females prepared by injecting 5 IU each of pregnant mare serum gonadotrophin (PMSG) (Sigma Aldrich) and human chorionic gonadotropin (hCG ...
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bioRxiv - Bioengineering 2021Quote: ... The primary antibody buffer was 3% horse serum and 5% DMSO dissolved in 1X PBS with 0.2% Tween-20 (Sigma Aldrich, P2287) and 10 μg/mL heparin (Sagent Pharmaceuticals ...
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bioRxiv - Neuroscience 2020Quote: ... cell death caused by drugs were detected by double staining the cells with 5 μl Hoechst 33342 (23491-52-3, Biotech, China) and Propidium iodide (PI) (P4170, Sigma, USA) for 15 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked at room temperature with either 5% (w/v) non-fat milk or 3% (w/v) BSA (Sigma- Aldrich) in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: ... sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418, SIGMA)) ...
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bioRxiv - Microbiology 2021Quote: Metabolites were extracted from cell pellets through addition of 50 μl chloroform and vigorous vortexing, followed by 200 μl methanol/ultrapure water (3:1, containing 5 μM scyllo-inositol (Sigma I8132) as internal standard ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Cell Biology 2021Quote: ... validated lentiviral constructs (pLKO.1) expressing Mission shRNA targeting the following region 5’-GCTAGATTTACTAATGCCA-3’ in CPAP or scrambled shRNA were purchased from Sigma-Aldrich. For generation of stable cells ...
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bioRxiv - Molecular Biology 2022Quote: Human hPSCs cultured on MEFs were harvested using collagenase IV as big aggregates and settled 3 times in washing media (DMEM [Thermo Fisher Scientific], 5% Newborn Calf Serum [Sigma] ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was resuspended in lysis buffer (100 mM Tris, pH 7.4, 150 mM NaCl, 5 mM imidazole, 3 mM βME, and protease inhibitor cocktail from Sigma-Aldrich: P8849), lysed by sonication ...
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bioRxiv - Developmental Biology 2022Quote: ... RNAs immersed in Trizol were added to phase-lock heavy gel tubes (Brinkmann Instruments, INC., #955-15-404-5) and mixed with 100 uL of 1-bromo-3-chloropropane (BCP) (Sigma, #B9673), followed by room temperature incubation for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 6-Bromo-4-((dimethylamino)methyl)-5-hydroxy-1-methyl-2-((phenylthio)methyl)-1H-Indole-3-carboxylic acid ethyl ester monohydrochloride (Arbidol) (Sigma Aldrich) was dissolved in ethanol at 10mg/ml and diluted to target concentration in infection media.
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bioRxiv - Cell Biology 2022Quote: ... Printed discs were incubated in 0.1 mg/mL FITC-labelled dextran molecules (size either 3-5 kDa, 10 kDa, 20 kDa or 70 kDa (Sigma-Aldrich)) dissolved in PBS with 20mM CaCl2 (Sigma ...