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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the collected droplets were broken with 1H,1H,2H,2H-perfluoro-1-octanol (Sigma-Aldrich) to collect the capsules ...
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bioRxiv - Bioengineering 2022Quote: ... PDMS moulds were then functionalised using Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma, 448931). A few drops of silane were placed on a glass petri dish and into a desiccator together with the PDMS moulds ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 ml of fresh neuron culture media containing 1 μM 4-hydroxytamoxifen (4-OHT; Sigma H6278) was added into each well of neuron culture ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured for 4 hours with 4 µM monensin (Sigma-Aldrich, Saint Louis, MO, USA), fixed ...
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bioRxiv - Cell Biology 2021Quote: ... Cytokines: interferon gamma (IFNγ) (SRP3058) and human Interleukin-4 (IL-4) (I4269) were from Sigma-Aldrich, UK ...
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bioRxiv - Cell Biology 2021Quote: ... IP was performed by incubating 4 hours at 4°C with Flag M2 agarose beads (Sigma). Finally ...
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bioRxiv - Plant Biology 2022Quote: ... The quantification of GUS staining was done using 4-methylumbelliferyl ß-D-glucuronide (4-MUG; Sigma) as a substrate with excitation at 365 nm and emission at 455 nm read using a plate reader POLARstar Omega (BMG Labtech ...
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bioRxiv - Developmental Biology 2022Quote: ... a single dose of 1 mg of 4-hydroxytamoxifen (4-OHT, H7904, Sigma, ≥98% Z (trans) isomer ...
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bioRxiv - Neuroscience 2022Quote: Fixation was performed following 24 hrs of recombinant protein treatment using 4 % PFA / 4% Sucrose (Sigma) in 1x PBS at RT for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe (strain SHY1058) cultures were labeled with 5 mM 4-thiouracil (4-thioU) (Sigma-Aldrich #440736) for 4 min ...
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bioRxiv - Genomics 2022Quote: ... 0.01% [w/w] 4-hydroxy- 2,2,6,6-tetramethylpiperidin-1-oxyl (4-hydroxy-TEMPO, Sigma-Aldrich, cat# 176141), 0.2% [w/w] tetramethylethylenediamine (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Animals expressing inducible Cre (CreERT2) received a single injection of 4-hydroxytamoxifen (4-OHT; Sigma #H6278) on P21 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μl of 0.5 nmol α-Cyano-4-hydroxycinnamic acid (4-CIN, Sigma Aldrich, Cat #: C2020) dissolved in ACSF containing DMSO (Wang ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1 % tetramethylethylenediamine (TEMED, Roth) and 0.005 % 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (4-hydroxy-TEMPO, Sigma) were added to activate the polymerization reaction ...
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bioRxiv - Cell Biology 2023Quote: Cells were fixed with ice-cold (4°C) DMEM containing 4% paraformaldehyde (Sigma-Aldrich, Cat# P6148) for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: Activity-dependent recombination was triggered using 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich, Catalog no. H6278, USA). A 20 mg/ml solution of 4-OHT was prepared in ethanol by shaking at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Cre-mediated recombination in the organoids was induced by adding 4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich; final concentration 2 µM ...
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bioRxiv - Genomics 2024Quote: ... Cells were then fixed with 4% PFA and stored at 4 °C in PBS (Sigma-Aldrich). For scRNA-seq and snATAC-seq ...
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bioRxiv - Developmental Biology 2024Quote: ... and then fixed for 48 hours at 4 °C in a 4% paraformaldehyde solution (Sigma-Aldrich). Following fixation ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 cells at ∼70% viability were spun down and incubated 3 min at 4°C in 25µl of a detergent buffer containing 0.2% Igepal CA-630 (Sigma, #I8896), 0.2% Tween 20 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Retinal organoids (n=3-4) for each time point were homogenized using a Dounce Tissue Grinder (Sigma-Aldrich, UK) and processed using SYBR™ Green Fast Advanced Cells-to-CT ™ Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium (BCIP /NBT) liquid substrates for AP-enzyme (SIGMA) were added for overnight incubation at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (ammonium salt) (PI(4,5)P2) (Sigma 850155P), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed in PBS and incubated with 3 μg/ml DAPI (4’,6-diamidino-2-phenylindole; D8417 from Sigma Aldrich) at RT for 30min ...
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bioRxiv - Physiology 2020Quote: The reduction of yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Germany) was used to measure cellular metabolic activity as a proxy for cell viability [19,22] ...
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bioRxiv - Neuroscience 2020Quote: ... They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane, 2 membranes per animal, 0.4 μm Millicell, Merck Millipore) in 50% BME (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2 6 H2O and 5-Bromo-4-chloro-3-indolyl β-D-galactoside or X-gal (Sigma, B4252 ...
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bioRxiv - Plant Biology 2021Quote: ... Col-0 plants grown on soil for 3-4 weeks were spray-treated with 0.5 mM SA (Sigma-Aldrich) and tissues harvested at indicated time-points for further analysis.
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally washed 3 times in PBS-Tween 20 0.1% and mounted in Mowiol 4-88 (81381, Sigma).
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bioRxiv - Neuroscience 2022Quote: ... GLASTCreERT2;Sun1-GFP mice at ∼3 weeks of age were intraperitoneally injected with 4-OHT (Sigma-Aldrich, #H6278-50mg) in corn oil (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... organoids in Matrigel on day 3 or 4 after passage were incubated with 100 μM rhodamine 123 (Sigma-Aldrich) in HBSS (Wako ...
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bioRxiv - Cancer Biology 2022Quote: Human melanoma cells were seeded in 6-well plates with 0.1-1 mL lentivirus in a final volume of 3 mL with 4 µg/mL polybrene (Millipore). Cells were spun at 1,000 x g for 2 h at 30 ºC ...
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bioRxiv - Molecular Biology 2021Quote: ... The unbroken cells were removed by centrifugation (3,000 x g; 4°C; 5 min; Sigma 3-16KL; rotor 11180). The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 250 µL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) (20 mg/ml) (Sigma-Aldrich, UK) and Isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2022Quote: A model of hyperalgesic priming was used to induce chronic widespread pain in mice with consecutive injections of carrageenan into the right gastrocnemius muscle 4 days apart (i.m. 3% 30μl, Sigma Aldrich). Control mice only received one injection on D0 (30).
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bioRxiv - Immunology 2021Quote: ... and two pools of S2 peptides (pools 3 and 4) (final concentration of 2μg/mL) on IPVH-membrane plates (Millipore) coated with 5μg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Developmental Biology 2020Quote: ... were post-fixed with 4% para-formaldehyde and blocked with PBS solution containing 0.3% Triton-X 100 and 3% normal Donkey serum (Sigma). Antibody dilution buffer consisted of PBS containing 1% BSA and 0.3% Triton-X 100 ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell disruption was carried out by vortexing (3 cycles, 5 min each) at 4°C using 0.2 ml of glass beads (425-600 μm; Sigma). For the Rpb3 immunopurification ...
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bioRxiv - Cell Biology 2022Quote: ... was revealed with 5-bromo-4-chloro-3-indolyl β-D galactoside (X-gal) (Sigma-Aldrich, Carlsbad, CA, USA). Diploid cells were grown on SC medium supplemented with 20 mg/L adenine hemisulfate ...
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bioRxiv - Microbiology 2024Quote: ... Coloring reactions were performed with nitro blue tetrazolium/5-Bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) solution (Sigma), and then sections were washed in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
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bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
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bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...
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bioRxiv - Biochemistry 2024Quote: ... Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP, Sigma Aldrich) dissolved in 100 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged MSL proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Elution buffer ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were concentrated to ∼ 3-4 mg mL-1 using a 100 kDa MWCO centrifugal concentrator (Millipore Sigma) for cryo-EM sample preparation.
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bioRxiv - Cell Biology 2023Quote: The cell proliferation assay was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay (Sigma). All cancer cell lines were seeded in 24-well plates (2×104 cells/well) ...