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Citations for Millipore Sigma :
7901 - 7950 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Pyruvate (100 mM; Millipore), and UDP-GlcNAc (77 mM ...
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bioRxiv - Microbiology 2024Quote: ... Uridine (100 mM; Millipore), Pyruvate (100 mM ...
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bioRxiv - Cell Biology 2024Quote: 100% Ethanol (Sigma #E7023)
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bioRxiv - Cell Biology 2024Quote: ... 100 µM blebbistatin (Sigma) and 10 µM ML7 (Tocris Bioscience) ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... TritonX-100 (T9284500ML, Sigma), tryptone (DF0123-17-3 ...
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bioRxiv - Biochemistry 2019Quote: ... 300 µl of 10% methanol (LC-MS grade) spiked with stable isotope-labelled amino acids (L-amino acid mix, Sigma-Aldrich, Co., St. Louis, MO, USA) was added to each sample ...
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bioRxiv - Neuroscience 2021Quote: Cortices (∼250 mg each) were dissected from freshly removed brains in ice cold PBS containing 100 µg/ml cycloheximide (CHX; Sigma-Aldrich, catalog # 01810). The tissue was then cut into smaller pieces with a sterile scalpel and Dounce homogenized on ice in lysis buffer (5 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... 1mM DTT) supplemented with 100 µg/mL lysozyme and 1 tablet of EDTA-free complete protease inhibitor (Sigma-Aldrich, St. Louis, MO, USA). Resuspended cells were lysed by sonication ...
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bioRxiv - Genomics 2020Quote: ... microglia differentiation media was exchanged with HEK293T media (DMEM + 10% heat-inactivated FBS + final 2mM L-Glutamine) and supplemented with 100 hg/ml lipopolysaccharide (LPS, Sigma Aldrich L4391-1MG) or PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
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bioRxiv - Neuroscience 2021Quote: ... 0.8 M guanidinium chloride, 8 U/ml proteinase K, 0.5% Triton X-100, pH 8.0; all chemicals are from Sigma-Aldrich, St. Louis, MO, USA), and incubated ON at 50°C in a humified chamber.
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bioRxiv - Cancer Biology 2021Quote: ... were grown in Dulbecco’s 90% Dulbecco’s MEM (4.5 g/L glucose) with penicillin– streptomycin (100 U/ml) and 10% fetal bovine serum (Sigma-Aldrich, St Louis, MO, USA) in 5% CO2 cell culture incubator ...
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bioRxiv - Cancer Biology 2021Quote: ... The cross-links were reversed by overnight incubation at 65 °C in the presence of Proteinase K (100 μg/ml; Sigma-Aldrich; Cat#: P2308). After cross-link reversal ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 mg/mL of lyophilized powder or controls in Millipore water were added to 100 mg/L indigo carmine (Sigma-Aldrich catalog 131164) and incubated at RT ...
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bioRxiv - Plant Biology 2020Quote: ... 20 μL of (GlcNAc)6 was added in a final concentration of 10 μM to trigger ROS production in the absence or presence of 100 μL of effector protein in a final concentration of 50 μM in measuring solution containing 100 μM L-012 substrate (FUJIFILM, Neuss, Germany) and 40 μg/mL horseradish peroxidase (Sigma-Aldrich, Missouri, USA). Chemiluminescence measurements were taken every minute over 30 min in a CLARIOstar microplate reader (BMG LABTECH ...
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bioRxiv - Cell Biology 2020Quote: ... The PDMS coated surface of the 50 mm FluoroDish dish as well as the side of the coverslip bearing the PDMS pillars structures were incubated with 100 μg/ml BSA (Sigma-Aldrich, #A9647-100G) in PBS for 30 min at room temperature ...
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bioRxiv - Physiology 2022Quote: ... were used to induce elevated apoptosis and permeability.[29] The cells were grown for 5 days before incubation with TNF-α and chx (TNF-α/chx) in the presence or absence of the RS isoforms or heparin (100 µg/mL; Sigma-Aldrich). TNF-α/chx was removed after 3.5 h of incubation and the cells were allowed to recover for 20 h in the presence or absence of RS or heparin.
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bioRxiv - Immunology 2021Quote: 6 × 106 cells/channel of human umbilical vein endothelial cells (HUVECs, ATCC-PCS-100-013) were seeded into the fibronectin (50 μg/mL, Sigma-Aldrich cat. # F0895) coated channels of a 48-well microfluidic plate (Bioflux ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The pellet was resuspended in 1 mL PBT (1X PBS, 0.1% Triton X-100, 2% BSA, 0.2U/µL protease inhibitor (Sigma Aldrich cOmplete protease inhibitor tablet), and 0.2 U/µL SUPERase-in RNAse inhibitor (Invitrogen)) ...
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bioRxiv - Plant Biology 2019Quote: ... surface sterilized and stored at 4 °C for 3 days before growing on MS agar (1.1% w/v) containing 100 µg/mL kanamycin (Sigma-Aldrich, St. Louis, MO). The proportion of WT to dgk4-1 seeds were scored after 7 days of growth.
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bioRxiv - Bioengineering 2020Quote: ... The lower layer of the trans-well plate was filled with 100 ng/ml of HMGB1-containing DMEM with various concentrations of FSP-ZM1 or AMD3100 (Sigma, St Louise, USA). After 24 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then 4 µL of ligation mix was transformed into 50 µL of home-made chemically competent E.coli DH5alpha obtained from New England Biolabs (C2987I) and selected on LB Agar plates containing 100 µg/mL Ampicillin (Sigma-Aldrich, A0166-25G)
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bioRxiv - Synthetic Biology 2021Quote: ... Clones were selected with lacZ based blue-white screening method on LB agar plates containing 100 µg/mL Ampicillin (Sigma-Aldrich, A0166-25G), 100 µM IPTG (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2021Quote: ... spun down again and resuspended the cells in 200 µL of 1X PBS (phosphate buffered saline) with 1.5 μl 4′,6- diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 100 ng/ml). DAPI stains dead cells ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were propagated at 37°C with agitation to an OD600 of 0.6 in modified M9 minimal medium containing 100 μg/mL ampicillin (Sigma Aldrich, St. Louis, MO), 1 g/L NH4Cl (or 15NH4Cl for NMR (Cambridge Isotopes ...
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bioRxiv - Microbiology 2022Quote: ... Cells were harvested ~24 hours after transfection and resuspended in 500 µl of lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10% glycerol, 0.5% IGEPAL, 100 μg/ml RNaseA, 1 tablet of Sigma-Aldrich cOmplete Protease Inhibitor Cocktail). Resuspensions were vortexed ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue sections pre-stained with DiIC18 for the indicated time were incubated for 30 min at RT with 100 µg/ml digitonin (Sigma Aldrich; cat. D141) dissolved in 3% bovine serum albumin (BSA)/PB ...
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bioRxiv - Microbiology 2024Quote: ... Monocyte-derived macrophages were derived by treating the cell lines with 100 ng/ml of phorbol 12-myristate 13-acetate (PMA; Sigma, St. Louis, MO) for 48 h followed by a 24 h incubation in medium alone before experiments ...
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bioRxiv - Cell Biology 2024Quote: ... permeabilized with 100 μL of permeabilization buffer (0.5% TritonX-100 in PBS) for 1 hour with shaking at room temperature and counterstained with DAPI (Sigma-Aldrich, 10 μg/ml) in 0.1% TritonX-100 in PBS for 45 minutes ...
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bioRxiv - Cell Biology 2024Quote: BODIPY probe fluorescence intensity decay curves were also recorded in colloid solutions containing different concentrations (10, 20, 50, 100 and 200 mg/ml) of porcine stomach mucin Type II (Sigma-Aldrich, ON, Canada). Mucins isolated from porcine stomach were dissolved in 1 M NaOH and the pH subsequently adjusted to ∼2 with HCl 56.
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bioRxiv - Physiology 2024Quote: ... the excess of reagents was eliminated by performing several washing steps with Milli-Q water using 4 mL cellulose membrane centrifugal filters (Amicon, MilliPore, 100 kDa, Merck). All MNP suspensions were sterilised by filtration using 0.22 μm MilliPore® filters before addition to cell cultures ...
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bioRxiv - Neuroscience 2024Quote: HEK293T cells cultured in 60 mm dishes were lysed in 1 ml PBS with 1% TritonX-100 plus protease inhibitors (cOmplete™, Mini Protease Inhibitor Cocktail, Sigma-Aldrich #11836153001) and phosphatase inhibitors (1mM NaPPi ...
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bioRxiv - Immunology 2023Quote: Thawed PBMCs were incubated with 100 µl of a stimulation mixture containing 5 ng/ml PMA (Sigma-Aldrich, St Louis, MO; P1585-1MG) and 500 ng/ml ionomycin (Sigma-Aldrich ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 37°C and 5% CO2 for 48 hours in RPMI 1640 containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma, St. Louis, MO, USA), 2mM L-Glutamine (Thermo-fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were collected by centrifugation at 2500 x g for 5 min and resuspended in 1 mL of infiltration buffer (10 mM MgCl2:6H2O, 10 mM MES-KOH, 100 μM acetosyringone (Sigma-Aldrich, Missouri, USA)) ...
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bioRxiv - Microbiology 2023Quote: ... Phage was concentrated to 1 mL via ultrafiltration using Amicon™ 100 K Ultra-15 Centrifugal Filters (Sigma-Aldrich, St. Louis, Missouri, USA) at 4 ...
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bioRxiv - Neuroscience 2023Quote: ... the isolated ganglia were immediately transferred into a 24-well plate containing digestion solution (1 ml high glucose DMEM and 100 μl Collagenase Type IV (Sigma; C1889-50 mg) and incubated at 37°C for 40 min ...
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bioRxiv - Cell Biology 2023Quote: ... The following reagents were used for cell treatments at given concentrations: Lipopolysaccharide (Escherichia coli O55:B5, #L2880, Sigma-Aldrich, 100□ng□ml-1), recombinant human TNFα (rHuTNF ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in double filtered sea water for 60 minutes on a shaking plate followed by three 1 minute 1X PBS Tween (1X Phosphate Buffered Saline: Gibco #70011-044, 0.5% Tween 20: Sigma-Aldrich P1379-100 mL) washes followed by three 10 minute 1X PBS Tween washes ...
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bioRxiv - Genetics 2023Quote: ... all His-SIRT5 were equalized to 320 µg/ml with exchange buffer (25 mM Tris/HCl pH 8.0, 100 mM NaCl) containing 20x Sypro Orange (#S5692, Sigma-Aldrich, delivered at 5000x). Samples (25 µl ...
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bioRxiv - Genomics 2023Quote: ... 10 mL ATCC vitamin supplement and 1 mL vitamin K-3 solution (0.14 g vitamin K-3 in 100 mL 95% ethanol)24 reduced by the addition of 50% Oxyrase (SAE0013; Sigma, St. Louis, MO) were added in an anaerobic chamber ...
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bioRxiv - Microbiology 2023Quote: ... and 100 μL per well virus solution with or without 1μg/mL TPCK-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA, Cat# 4352157) was added per well ...
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bioRxiv - Cell Biology 2023Quote: ... the Anti-Flag M2 Affinity Gel was incubated with 2x vol of 100 µg/ml Flag-Peptide (in TBS, Sigma-Aldrich, cat.no. F3290) at 4 °C and 350 rpm agitation for 30 min ...
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bioRxiv - Plant Biology 2024Quote: ... followed by a final wash using the LiCl wash buffer (0.25 M LiCl, 1% (v/v) NP-40 (Sigma-Aldrich NP40S-100 mL), 1% Sodium Deoxycholate ...
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bioRxiv - Cell Biology 2022Quote: ... 1% Triton-X-100) supplemented with phosphatase inhibitor (1:100 P5726, Sigma Aldrich) and protease inhibitor (1:10 11836170001 ...
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bioRxiv - Neuroscience 2020Quote: ... 10, 15, 10, 10 min) followed by propylene oxide (100%, 100%) (Sigma-Aldrich), infiltrated first with a 50/50 propylene oxide/epoxy mixture ...
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bioRxiv - Bioengineering 2024Quote: ... cells were permeabilized with 0.1% Triton® X-100 (Sigma-Aldrich, X-100) in PBS for 10 mins ...
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bioRxiv - Microbiology 2023Quote: Pelleted interphases were resuspended in 100 µL of 100 mM TEAB (Sigma-Aldrich) and reduced with 20 mM DTT (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... cells were permeabilized with 0.1% Triton® X-100 (Sigma-Aldrich, X-100) in PBS for 10 mins ...