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Citations for Millipore Sigma :
7851 - 7900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... cells were labeled with Phalloidin-Tetramethyl rhodamine-B (1/1000) (Sigma-Aldrich) for 1 hour at RT and Hoechst (1X ...
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bioRxiv - Cell Biology 2024Quote: ... and detected by western blotting using anti-tubulin (Sigma, T5168, 1∶1000) and anti-GFP (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: mouse anti-PV (1:1000, Sigma, #P3088), rabbit anti-PV (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-Fractin (N-terminal fragment of actin) (1:1000, Millipore, AB3150) Abs were incubated with agitation in PBT-BSA for 1 hr at RT and 72 hrs at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used included: rabbit anti-Nervana 2.1 (1:1000; Sigma Millipore) and mouse anti-Futsch/22C10 (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used included: rabbit anti-Nervana 2.1 (1:1000; Sigma Millipore) and mouse anti-Futsch/22C10 (1:2000 ...
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bioRxiv - Genetics 2021Quote: ... coli OP50 supplemented with or without vitamin B12 (methylcobalamin or MeCbl, Sigma, Cat #13422-55-4; adenosylcobalamin or AdoCbl, Sigma, Cat #13870-90-1) and propionic acid (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... glycerol stock containing the uropathogenic Escherichia coli strain UTI89 was used to inoculate a Miller Luria Broth (LB) agar plate (Sigma-Aldrich, St. Louis, MO). The plate was incubated for 24 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... mice were weighed and immediately administered an intraperitoneal injection of lipopolysaccharide (7.5 × 105 EU/kg) from Escherichia coli O111:B4 (Millipore Sigma, Temecula, CA; Cat# L4391). Two hours post-LPS injection ...
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bioRxiv - Immunology 2020Quote: ... Classically activated M1 phenotype was achieved by exposure to 100 ng/mL LPS from Escherichia coli O111:B4 (Sigma Aldrich; St. Louis, MO, USA) and 20 ng/mL recombinant human IFN-γ (PeproTech ...
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bioRxiv - Immunology 2023Quote: Human FM explants were treated with either a no treatment (NT) media control or 100ng/ml LPS isolated from Escherichia coli O111:B4 (Sigma-Aldrich, St Louis, MI) for 48 hours ...
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bioRxiv - Immunology 2022Quote: ... 2-4×104 bone marrow-derived DCs (BMDCs) were re-plated in 96-well round-bottom plates and stimulated with 1.0 μg/ml LPS (Sigma L2762: Escherichia coli 26:B6) and different concentrations of peptides for 24 hours ...
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bioRxiv - Immunology 2023Quote: Propionate SCFA (catalog number P1880) and lipopolysaccharide (LPS, catalog number L4391, from Escherichia coli O111:B4) were purchased from Sigma-Aldrich (St. Louis, MO). Seahorse XF calibrant solution (part number 100840-000) ...
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bioRxiv - Immunology 2023Quote: ... Lipid A purified from Salmonella minnesota (Re-595) and lipopolysaccharide (LPS) from Escherichia coli (O55:B5) were purchased from Sigma-Aldrich (Merck, Darmstadt, Germany). Pam3CSK4 ...
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bioRxiv - Biochemistry 2021Quote: ... lung or intestinal tissue were fluorescently labeled via reductive amination with 2-aminobenzamide (2-AB) (Sigma-Aldrich Canada)42 ...
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bioRxiv - Biophysics 2022Quote: ... HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl] ethanesulfonic acid) was purchased from EMD Millipore (Billerica, MA USA) and NaCl (sodium chloride ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal bovine serum (FBS) and 1 ng/ml fibroblast growth factor-2 (FGF-2; Millipore). Cells were grown in tissue culture treated 24 well plates and 5% CO2 at 37°C.
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bioRxiv - Microbiology 2019Quote: ... 2 % [w/v] glucose) with or without 2 mM of t-BOOH (Luperox® TBH70X, Sigma-Aldrich, USA) and approx ...
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bioRxiv - Microbiology 2019Quote: ... using either 2-Deoxy-D-galactose (DOG) at a final concentration of 2 mg/mL (Sigma-Aldrich, D4407) or Azidothymidine (AZT ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5 µL g-1 solution of the photoinitiator 2-hydroxy-2-methyl propiophenone (Irgacure 1173; >97 %, Sigma-Aldrich) was added at 4°C temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM lipids: POPC: POPE: cardiolipin with a 2:2:1 weight ratio) and 160 μL MSP1D1 (Sigma, 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were suspended in ice cold HBSS (2% serum) containing 2 μg/ml Propidium iodide (Sigma-aldrich,USA) for dead cell discrimination ...
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bioRxiv - Cell Biology 2020Quote: ... TEBP-1-His5 and TEBP-2-His5 were expressed in Rosetta 2 (DE3) pLysS Competent Cells (Novagen,#71401). An overnight culture was grown in LB containing the respective antibiotic ...
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bioRxiv - Biophysics 2020Quote: ... Cystamine (Cys) and N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES ≥ 99.5 %) were also purchased from SIGMA. All samples were prepared in 0.1 M HEPES buffer ...
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bioRxiv - Microbiology 2019Quote: ... while intracellular GBS were recovered after 2 h infection and 2 h incubation with 100 μg gentamicin (Sigma) and 5 μg penicillin (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... 1.5 μL g−1 hydrogel of the photoinitiator 2-hydroxy-2-methyl propiophenone (Irgacure 1173; >97 %, Sigma-Aldrich) were mixed in at a temperature of 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... samples were incubated with 2 % (v/v) primary anti-tubulin antibody (clone B-5-1-2, Sigma-Aldrich) in blocking buffer for 60 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 2 ml of PBS with 2 mM EDTA and 25 μM cisplatin (Sigma-Aldrich, St. Louis, MO, USA) was added to the cells ...
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bioRxiv - Microbiology 2022Quote: ... 150 μM (2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide (EF5 compound; Sigma-Aldrich, EF5014), 0.5 μM MitoSOX™ fluorescent probe (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... To asses mitochondrial fuel usage OCR was measured subsequent to the addition of the following drugs in different combinations as indicated: 3 μM bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES) (SML0601, Sigma), 2 μM UK-5099 (PZ0160 ...
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bioRxiv - Neuroscience 2022Quote: ... the noradrenergic neurotoxin N-(2-Chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP4) (10 mg/kg, i.p., Sigma-Aldrich) or vehicle (0.9% saline ...
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bioRxiv - Developmental Biology 2023Quote: Stock solution of nocodazole (2 mg/mL) was prepared by mixing 2 mg of nocodazole (Sigma-Aldrich, #M1404) with 1 mL of DMSO (J.T.Baker ...
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bioRxiv - Biochemistry 2022Quote: ... also contained 1-2 µg/uL α-MBPAF546 or 1-2 µg/uL mouse α-BRCA2 (Ab1, Millipore) plus 1-2 µg/µL goat α-mouse IgGAF546 (Molecular Probes).
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bioRxiv - Bioengineering 2023Quote: ... in 100 mM HEPES buffer (N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid, pH 5.25, Sigma, St. Louis, MO) mixed with 3.5 kDa PEG-DT (JenKem ...
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bioRxiv - Cell Biology 2023Quote: ... N=4 (2 females, 2 males) mice were given 12.5 mg/kg sodium nitrite (52mg/L, 23713, Sigma) in 10% blackcurrant squash (Robinsons) ...
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bioRxiv - Microbiology 2023Quote: ... Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) and 2-chloroacetamide (CAA) used for proteomics analysis were from Sigma Aldrich. The Bicinchoninic acid (BCA ...
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bioRxiv - Molecular Biology 2023Quote: Metabolic quenching and nucleotide phosphate metabolite pool extraction were performed by adding 1 ml ice-cold 2:2:1 acetonitrile:methanol:water with 0.1% formic acid with 1x MS-Stop phosphatase inhibitor (Sigma-Aldrich). Samples were spiked with 10 µl 100 mM mix of adenosine-15N5-5′-triphosphate ...
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bioRxiv - Bioengineering 2023Quote: ... cells were induced for Ngn2 and DjCas13d expression using 2 ug/mL Doxycycline (2 ug/mL, Sigma D3072). On day 3 ...
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bioRxiv - Biochemistry 2022Quote: ... 5-Fluoro- 2’-deoxycytidine and 6-thio-2’-deoxyguanosine were obtained from Sigma-Aldrich (Saint- Louis, MO, USA). Phosphodiesterase I was purchased from Affymetrix (Cleveland ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then incubated for 2 hours with 3 µg/ml 5-bromo-2’-deoxyurine (Sigma-Aldrich, B5002). Cells were then detached using trypsin/EDTA (GIBCO ...
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bioRxiv - Physiology 2023Quote: ... mice were treated with a single dose of 2 mg 5-ethynyl-2′-deoxyuridine (EdU, 900584, Sigma Aldrich) in PBS by intraperitoneal injection 2 h before euthanasia by cervical dislocation ...
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bioRxiv - Microbiology 2023Quote: Cells were lysed with 2 × Laemmli sample buffer (Bio Rad Laboratories, CA, USA) containing 10% 2-mercaptoethanol (Sigma) and boiled for 10 minutes at 100℃ ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total of 150 µL of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone; Sigma-Aldrich) dissolved in 100% ethanol (Wako ...
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bioRxiv - Cancer Biology 2024Quote: ... we used 2’,7’-Bis (2-carboxyethyl)-5 (6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) (Sigma-Aldrich, Darmstadt, Germany). Cells were starved overnight ...
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bioRxiv - Plant Biology 2020Quote: ... phosphatase inhibitor cocktail 2 (Sigma-Aldrich; P5726) and phosphatase inhibitor cocktail 3 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... 0.5% Phosphatase inhibitor cocktail 2 (Sigma-Aldrich). MAPK phosphorylation was determined using an anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Biophysics 2021Quote: ... Zn(NO3)2 (0.5 mM; Sigma-Aldrich), Mg(NO3)2 (0.5 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.1% 2-mercaptoethanol (Sigma Cat#M-7522), 500,000U Recombinant Mouse LIF Protein (Millipore Cat#ESG1107) ...
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bioRxiv - Biophysics 2020Quote: ... 2% MEM amino acids (50x, M5550, Sigma), 1% Penicillin Streptomycin (10,000 units/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-glutamine (G7513, Sigma-Aldrich), and penicillin/streptomycin (P0781 ...