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Citations for Millipore Sigma :
7851 - 7900 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... gondii antibodies detection was conducted using an anti-monkey IgG FITC conjugate produced in rabbit (Sigma-Aldrich®, USA). T ...
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bioRxiv - Microbiology 2023Quote: ... and cells were probed with anti-FLAG polyclonal primary antibody produced in rabbit (Sigma-Aldrich Cat# F7425, RRID: AB_439687) overnight at 4° C ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with anti-FLAG polyclonal primary antibody produced in rabbit (Sigma-Aldrich Cat# F7425, RRID: AB_439687) at 4° C overnight Then the membrane was washed 3 times in PBS-T (0.5% tween 20 in 1 x PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... blocking solution was removed and the sample was incubated in anti-fibronectin I antibody from rabbit (Sigma-Aldrich, #F3648), diluted 1:500 in blocking solution ...
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bioRxiv - Microbiology 2024Quote: ... They were then incubated for 1h with an anti-rabbit IgG conjugated to horseradish peroxidase (HRP) (A9169; Sigma-Aldrich) as the secondary antibody at a dilution of 1:10,000 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubating with the secondary antibodies (anti-Mouse MINUS, DU092004, Sigma-Aldrich; anti-Rabbit PLUS, DUO92002, Sigma-Aldrich) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were in blocking buffer (5.0% rabbit serum (Labtech) + 0.1% Triton TM X-100 (Sigma-Aldrich, Dorset, UK) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The proximity ligation reaction and visualization of signal was performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for rabbit and human antibodies (Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Biochemistry 2024Quote: ... anti-p-Histone H2A.X Ser139 (ψH2A.X, mouse monoclonal, 05-636) and pan-ADP-ribose binding reagent (rabbit Fc tag, MABE1016) were obtained from Millipore. anti-DHFR (rabbit polyclonal ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with primary antibodies overnight at 4C (DARPP-32 1:200 Abcam ab40801; FoxP1 1:500 Abcam ab16645; GAD65/67 1:500 Millipore AB1511; MAP-2 1:1000 Millipore MAB3418) and then washed three times with PBS before an overnight 4C incubation in secondary antibody (Alexa-fluor rabbit 555 IgG ...
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bioRxiv - Genetics 2021Quote: ... all diluted in a 1:1 mixture of PBT and 3% BSA: mouse anti-α-tubulin (1:500, Sigma T6199), rabbit anti-Spd-2 (1:4.000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% ITS-X supplement (WAKO) and purified BSA (3%, WAKO)) and N2B27 (DMEM/F12/GlutaMax + Neurobasal at 1 : 1 (Sigma), 1 × B27 (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... and then modified with 1:1 volume ratio with 1 mg/ml biotin-5-fluorescein conjugate (Sigma-Aldrich, cat #53608) followed by three washes with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... 1 g L−1 (NH4)2-H-citrate and 1 mL antifoam (Antifoam 204, Sigma-Aldrich, St. Louis, MO, USA). The medium was supplemented with with 2 mL L−1 trace elements solution and (1 M ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell Signalling Technology), ERK (1: 1000, Cell Signalling Technology), p-ERK (1: 1000, Cell Signalling Technology) and GAPDH (1: 5000, Sigma) overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... five flies of each sex and genotype were placed into a vial with 2 ml of 1 X dyed medium (2.5 % yeast, 2.5 % sucrose, 1 % agar and 1 % Brilliant Blue FCF – Sigma Aldrich, USA). After 2 h of feeding ...
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bioRxiv - Neuroscience 2019Quote: ... for the final differentiation in neural differentiating medium (NDM) (Neurobasal 1% NEAA, 1% N2 supplement and 1% P/S) supplemented with 1µM of cAMP (Sigma-Aldrich) and 10 ng/mL of BDNF ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or α/β-tubulin (1:1 mixture) (1:100 in PBS; 2 hr at 37°C) (Sigma; T9026, T4026, USA). FITC-conjugated anti-mouse IgG (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated in 5% horse serum in 0.5% Triton-X TBS for 1 hour before overnight incubation with 1:500 anti-GFP or 48 hours 1:300 anti-UBE3A (3E5 Sigma) and 1 hr 1:500 Alexa-Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... two media containing either commercialized mixed bile salts (0.5 g L−1, 1:1 sodium cholate: sodium deoxycholate, cat# 48305, Sigma-Aldrich) or a primary bile salts composition (0.25 g L−1 sodium cholate and 0.25 g L−1 sodium chenodeoxycholate ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Biochemistry 2020Quote: ... The full-length MtaLonA were prepared by supplementing the growing medium with 1 g l−1 of 15NH4Cl and 4 g l−1 of 2H7/12C6-glucose in 99.8%-2H2O (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... anti-γH2AX (JBW301, 1:1000), anti-P-Ser15-p53 (1:1000) and anti-ATM Ser1981 (10H11.E12, 1:200) were from Millipore. Anti-P-KAP1Ser824 (1:1000) ...
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bioRxiv - Developmental Biology 2021Quote: ... and then with Theobroma cacao extract fraction 5 (100 µg.mL-1) or (-)-epicatechin (80 µg.mL-1) (Sigma-Aldrich, HW101708-1) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS/0.1% Triton X-100 and incubated with the secondary antibody (1:500) and 1 mg ml−1 DAPI (Sigma, D9564) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... 1:100 mouse anti-ASCL1, Guillemot lab; 1:800 rat anti-BrdU, Abcam ab6326; 1:800 chicken anti-GFAP, Millipore AB5541 ...
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bioRxiv - Neuroscience 2020Quote: ... 12565501) with 0.01M phosphate-buffered saline and processed immediately for zonula occludins-1 (ZO-1; 1:500, Millipore Sigma, MABT11) and Bmal1 (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and transfer vector at 1:1:1 molar ratio using the X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich; # 6365787001) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl and 0.1% Tween 20) were performed before incubating the membrane with the anti-FLAG antibody 1:5000 for 1 h (Sigma). Next ...
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bioRxiv - Genomics 2022Quote: ... Cells are then resuspended in 10ml Sucrose/Glycerol buffer (1:1) (Sucrose Buffer: 0.3M Sucrose; 1% Triton-X (Sigma Aldrich); 10mM HEPES (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: Transfected COS-7 cells were washed three times with ice cold PBS and scraped in buffer K (1 mM Tris-HCl, 1 mM EGTA and 1 mM MgCl2, pH 7.6) containing protease inhibitor cocktail (Sigma, # P2714). Cells were mechanically lysed by passing them 10 times with 301/2-gauge needle and small fractions were used to measure the total protein using a Coomassie protein assay (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse monoclonal anti-Tau-1 (05-838, 1:1000), mouse monoclonal anti-MAP2 (05-346, 1:500), rat monoclonal anti-tubulin (MAB1864, 1:500) from Millipore; chicken polyclonal anti-GFP (ab13970 ...
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bioRxiv - Genomics 2020Quote: ... The blocking solution was aspirated and replaced with the same solution with primary antibodies (MAP2-Ck, Abcam ab5392, 1:500; Nanog-Gt, R&D AF1997, 1:200; TRA-1-60 IgM-Ms, Millipore MAB4360 ...
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bioRxiv - Plant Biology 2021Quote: ... 10%glycerol, 1 mM DTT, 1 mM EDTA, 0.5% Triton X-100 and 1% [v/v] protease inhibitor cocktail; Sigma-Aldrich). Insoluble debris was pelleted by centrifugation for 20 min with 4000 rpm at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... This wash step was repeated 3 times and the chloroform fraction was finally dried befor e being redissolved in 200 ml of 50% methanol prior to be loaded into a conditioned (1 CV methanol, 1 CV MiliQ water, 1 CV acetonitrile (Sigma) and 1 CV Mili-Q Water ...
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bioRxiv - Molecular Biology 2019Quote: ... and SmB(residues 1-91) [pCDFDuet-HT-B(1-91)-D3(1-75)] were constructed in a single pCDFDuet vector (Novagen) with N-terminal His(6)-tag followed by Tobacco Etch Virus (TEV ...
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bioRxiv - Genomics 2021Quote: ... The beads were washed two times with high-salt wash (50 mM Tris-HCl, pH 7.4, 1 M NaCl, 1 mM EDTA, 1% Igepal CA-630 (Sigma I8896), 0.1% SDS ...
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse monoclonal anti-Na+/K+ ATPase (1:500; SantaCruz) and anti-acetylated tubulin (1:2,000; clone 6-11B-1, Sigma Aldrich). Goat anti-mouse or rabbit IgG conjugated with HRP were used for secondary antibodies (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... and cultured with RPMI 1640 plus GlutaMAX + 1% (v/v) PS + 1% (v/v) B-27 + 1 μM thyroid hormone (T3) (Cat. No. T6397, Sigma) + 10 μM ALK5 inhibitor + 10 μM zinc sulfate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... the retinas were dissected and rinsed several times with 1× PBS and incubated for 15 minutes at 1:1000 dilution of PBS with 1 mg/ml Nile Red (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked with 5 % BSA/PBS for 1 h before overnight incubation with primary antibodies in 1 % BSA/PBS (RAB5 21435 1:200 Cell Signalling and anti-Integrin-β1 MAB1997 1:1000, Millipore) at 4°C prior to washing and incubation with secondary antibodies (anti-rabbit AlexaFluor-546 [A11-35] and anti-mouse AlexaFluor-633 [A21052] Thermofisher ...
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bioRxiv - Developmental Biology 2020Quote: ... buffered to pH 8.3 with 1 M bicine) supplemented with 1 g/l of and Antibiotic antimycotic solution (1:100, Sigma, A5955). To dissociate into single cells ...
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bioRxiv - Genetics 2021Quote: ... Samples were then digested for 15min at room temperature using an enzymatic solution (1% driselase, 0.5% cellulase, 1% pectolyase, 1% BSA, all from Sigma-Aldrich), and washed 3 times in PBS ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2021Quote: ... Positive transformants were selected on LB medium (1% tryptone, 0.5% yeast extract, 1% NaCl; Oxoid) supplemented with ampicillin (100 µg mL−1, Sigma Aldrich) for 24h at 37°C ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Samples were dehydrated with methanol series (50%, 75%, 95%, 100%, 100%) and overnight incubated in 1:1 methanol : BABB (BenzylAlcohol-BenzylBenzoate 1:2, both Sigma)) ...
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bioRxiv - Molecular Biology 2021Quote: ... infected in BGM containing Adeno-GFP (1×1010) or Adeno-ADH5 (1×1012) and differentiated in BGM containing 0.25nM 3-Isobutyl-1-methylxanthine (IBMX, Sigma, I5879), 1uM Rosiglitazone (Sigma ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of each culture supernatant were mixed with 50 μL of Griess reagent [1:1 (v/v) 0,1% N-(1-naphthyl) ethylenediamine dihydrochloride (NEED, Sigma-Aldrich) in water ...