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Citations for Millipore Sigma :
7701 - 7750 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Cumulus oocyte complexes were harvested 14 h after hCG injection and placed in human tubal fluid (HTF) medium supplemented with 4 mg/ml bovine serum albumin (BSA, A3311; Sigma-Aldrich, St. Louis, MO) covered with paraffin oil (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2023Quote: ... Then we added a series of two-fold dilutions of purified human SP-A (0-10 µg/ml, 100µl/well) in TBST containing either 5 mM CaCl2 (Sigma-Aldrich, Saint Louis, MO, USA) or 10 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: The following primary and secondary antibodies were used at indicated concentrations: anti-human α-actinin1 (mouse monoclonal, BM75.2, Sigma Aldrich, A5044; WB 1:1000), anti-human talin (mouse monoclonal ...
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bioRxiv - Cell Biology 2023Quote: 3μm sections of formalin fixed and paraffin-embedded (FFPE) human carotid artery samples from the Munich Vascular Biobank25 were mounted on 0.1% poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) pre-coated SuperFrost Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... the cells were spun down and the supernatants were collected for analysis by Human T Cell 17-plex panel (Sigma-Aldrich, Saint Louis, Missouri). For NK cell stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies ...
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bioRxiv - Genetics 2024Quote: ... Oviducts were dissected and oocytes from 2 animals were collected in 30 μl drops of HTF medium (EmbryoMax® Human Tubal Fluid Millipore, MR-070-D). Sperm was collected from 2 cauda epididymides in 50 μl drops of HTF medium ...
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bioRxiv - Biochemistry 2023Quote: McArdle RH7777 rat hepatocytes (McA cells) stably expressing human apoB100[31] were grown in culture plates coated with type I collagen from calf skin (Sigma Aldrich, St. Louis, MO) as previously described[32] and cultured in growth media composed of Dulbecco’s modified – Eagle’s medium (DMEM ...
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bioRxiv - Developmental Biology 2023Quote: ... Cumulus-oocyte complexes were placed in a 100-μL droplet of human tubal fluid medium supplemented with 4 mg/mL bovine serum albumin (BSA; A3311; Sigma Aldrich, St. Louis, MO) (Minami ...
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bioRxiv - Bioengineering 2024Quote: ... dissociated hiPSC-Cardiomyocytes and primary cardiac fibroblasts were mixed in a 3:1 ratio and resuspended in fibrinogen by mixing the cell solution with 33 mg/mL human fibrinogen (Sigma-Aldrich, cat. no. F3879) and RPMI-B27 (described above ...
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bioRxiv - Cell Biology 2024Quote: ... Serum-free DMEM was then discarded and cells were incubated in serum-free DMEM containing 80 µg/mL human HDL (Millipore, LP3, MW 175,000-360,000) or LDL (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies for DNA damage assays were diluted in PBS with 10% SuperBlock at the following dilutions: anti-human γH2AX Ser139 (EMD Millipore; JBW301; 1:200), goat anti-mouse IgG AF546 (Invitrogen ...
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Building a robust chromatin immunoprecipitation (ChIP) method with substantially improved efficiencybioRxiv - Plant Biology 2020Quote: ... and the sonicated chromatin was purified using protein centrifugal filters (30 kDa cutoff, Millipore, Billerica, MA, USA) to 300 µl ...
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bioRxiv - Plant Biology 2020Quote: Proteins were resolved on SDS-PAGE followed by immunoblotting using α-HA high affinity antibody 3F10 (Sigma) and α-c-Myc Antibody (9E10) ...
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bioRxiv - Biophysics 2021Quote: ... The His6-SUMO-C1Bδ fusion protein was expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma). For the natural abundance preparations ...
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bioRxiv - Cancer Biology 2021Quote: ... PreScission protease-eluted supernatant was added to 100 mL of S-protein agarose (EMD Millipore, 69704-3) to capture S-tagged protein ...
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bioRxiv - Biophysics 2021Quote: Cells were trypsinized using Trypsin-EDTA 0.05% and protein lysates were extracted in RIPA buffer (EMD Millipore) with Halt protease and phosphatase inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... where purified proteins (~10 μg) were run on TruPAGE precast 4–20% gradient acrylamide gels (Sigma-Aldrich) in TEA–Tricine running buffer with the Spectra Multicolor Broad Range protein ladder (ThermoFisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cell protein was extracted using a Tris-Triton lysis buffer with protease inhibitor cocktail (Sigma). Protein concentrations were assessed using a BCA assay (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... lysates were clarified and Flag-tagged protein were precipitated using anti-Flag M2 magnetic beads (Sigma Aldrich). After several washes in IP buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 µg proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membrane (PVDF, Millipore, IPVH00010). The membranes were incubated in blocking buffer (5% w/v nonfat dry milk in Tris-buffered saline ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by 8% to 12% SDS–PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Blocking and probing with antibodies were performed as previously described (Iréna Lassot et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with either HRP-linked goat anti-mouse IgG antibody (dilution 1:10,000; Sigma-Aldrich) or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown to 70% confluency before induction of protein expression using 1 μg/ml tetracycline (Sigma), and media supplementation with 50 μM biotin for protein labeling ...
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bioRxiv - Cell Biology 2020Quote: ... expression of EGFP fusion proteins was induced by addition of 1 μg/mL doxycycline (Sigma-Aldrich, D9891) and EGFP expressing cells were positively selected by FACS using a SONY MA900 to sort into media lacking doxycycline ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mg protein was incubated with 50 μl equilibrated anti-FLAG M2 affinity gel (Sigma–Aldrich, A2220) on a rotating wheel for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins (25-50µg) were resolved on 8 to 15% SDS-PAGE and blotted onto PVDF membrane (Millipore). Membranes were blocked for 1 h at room temperature with TBS-0.1% Tween20 and 5% milk or 1% bovine serum albumin (BSA) ...
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bioRxiv - Cell Biology 2020Quote: ... RNA immunoprecipitation was conducted using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma 17-700) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Target protein expression was detected using Immobilon™ Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were resolved by SDS/PAGE under reducing condition and transferred to PVDF membranes (Merck Millipore). After blocking ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were purified using anti-HA affinity matrix (Sigma; rat anti-HA clone 3F10 antibodies) and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... then 25 ug total protein was separated by 10% SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk and probed with the indicated primary antibodies following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Biophysics 2020Quote: ... Separated proteins were transferred to a nitrocellulose membrane and immunoblotted with an antibody recognizing Orai1 (Sigma Aldrich). Each experiment was performed at least 3 independent times and analysed with the program ImageJ (NIH ...
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein were run on SDS-polyacrylamide gel and transferred to Immobilon-P membrane (Millipore). Membranes were incubated with anti-YAP (Novus) ...
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bioRxiv - Molecular Biology 2022Quote: ... The centrifuged protein pellet was then resuspended in a urea/thiourea buffer containing protease inhibitors (PefaBloc; Sigma) prior to reduction with dithiothreitol and alkylation with iodoacetamide ...
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged RtcB proteins were affinity-purified from the lysates with anti-Flag M2 affinity-gel (Sigma) followed by extensive washes ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein quantification was performed using the BCA reagent according to the directions of the supplier (Sigma-Aldrich). Following β-mercaptoethanol (1% ...
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bioRxiv - Neuroscience 2021Quote: Both monomeric and fibrillar proteins were desalted first using the Amicon Centrifugal Filter (Millipore Sigma, Burlington, MA) before analyzed by ESI-MS ...
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bioRxiv - Molecular Biology 2021Quote: ... 4°C) and then incubated with pre-blocked Protein G beads (30ul of 50% slurry) (Sigma, USA) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, 0.2 μm pore, Millipore Corp., MA, USA) and incubated overnight at 4°C with primary antibody solution containing polyclonal rabbit anti-mouse ApoA-II antiserum (diluted 1:3000 ...
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bioRxiv - Immunology 2022Quote: ... All cells were incubated for 72 hours with Myelin Oligodendrocyte Protein (MOG) (2.5ug/ml; Sigma-Aldrich, M4939), with or without rIL-12 (25ng/ml ...
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bioRxiv - Genomics 2019Quote: ... Clarified lysates were incubated overnight with Protein A dynabeads conjugated to mouse anti-FLAG (Sigma Aldrich F3165) or anti-IgG antibody overnight at 4C ...
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bioRxiv - Immunology 2021Quote: ... 50μL of RIPA buffer for protein extraction ready-to-use-solution (Sigma-Aldrich®, Product No. R0278) containing Triton X100 1% and SDS 0.1% was added to the apical surface of AECs and incubated for 15 minutes on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore, Billerica, MA, USA) at 4,830× g at 4 °C for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... Effects on protein synthesis rates were assessed by SDS-PAGE and immunoblotting using puromycin antibody (Millipore, #MABE343).
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bioRxiv - Microbiology 2019Quote: ... The protein content was measured using the BCA Kit (Bicinchoninic Acid, Sigma-Aldrich, St Louis, MO, USA) and bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2019Quote: ... following which the proteins were transferred from gel to membrane and probed using Anti-FLAG antibody (Sigma).