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Citations for Millipore Sigma :
7701 - 7750 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Samples were prepared in fully deuterated 400 mM SPB for all measurements and contained ∼1 mM 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid (Sigma-Aldrich) as a frequency reference.
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bioRxiv - Cell Biology 2023Quote: ... Cells (or fibers) were then blocked with 3% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) diluted in PBST for 1 hr at room temperature to block non-specific binding sites ...
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bioRxiv - Cell Biology 2023Quote: ... Ovarian cells were isolated by enzymatic digestion in 3 mL of Dulbecco’s Modified Eagle’s Medium (DMEM) (cat# D6429, Sigma) containing 4 mg collagenase (cat# C5138-100MG ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, St Louis, MO, USA) was added to selected wells and cells were incubated at 37°C for 4h ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were extensively washed in lysis buffer and elution was carried out with 3×FLAG peptide (Sigma-Aldrich), HA peptide (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... in 3% (w/v) BSA were added for 1 hour before addition of 300 nM DAPI (Sigma Aldrich) and ProLong® Gold Antifade (Cell Signaling Technology) ...
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bioRxiv - Biochemistry 2024Quote: ... SidK(1-278)-3×FLAG (∼0.85 mg) was bound to 1.5 mL of M2-FLAG affinity gel resin (Sigma) and flow through was recirculated onto the column 6-7 times ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were washed 3 times in PBS before addition of 2% Triton X– 100 (Sigma-Aldrich) PBS solution to lyse the cells for enumeration of the intracellular bacteria ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then blocked in 0.45-μm filtered 3% (w/v) bovine serum albumin (BSA; Sigma-Aldrich A7906) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were washed in PBS and permeabilised in PBS supplemented with 3% FBS and 0.1% Saponin (Sigma-Aldrich) for 5 minutes at RT ...
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bioRxiv - Biophysics 2023Quote: ... antisense strand: 5’-GUA UCU CUU CAU AGC CUU A(dT)(dT)-3’) were synthesized by Sigma Aldrich. Early Endosome Antigen 1 (EEA1 ...
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bioRxiv - Cell Biology 2023Quote: ... Imidazole was removed by 3 cycles of concentration and dilution using Vivaspin (Millipore Sigma, Cat# GE28-9322-25). TEV enzyme was added at about 1/10 of the target protein mass and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... membranes spotted with different lipid species were blocked with 3% fatty acid free BSA (w/v; Sigma, 7030) in 0.1% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... the input media were centrifuged at 3,200 g for 30 min with 3 kDa MWCO filter units (Millipore); after centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitors (prepared in-house according to Phosphatase inhibitor cocktail 1, 2 and 3 from Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed with 0.5% glutaraldehyde and incubated for 3 min at RT with 5 mg/ml sodium borohydride (Sigma). After washes ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
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bioRxiv - Plant Biology 2024Quote: Leaves of 3-4-week-old Nicotiana benthamiana plants were agroinfiltrated and sprayed with 10 μM estradiol (Sigma) 24 h after infiltration to induce protein expression ...
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bioRxiv - Bioengineering 2023Quote: ... 1000 μM) prepared from a 0.5 M stock solution of 3-MB (Sigma-Aldrich, St Louis, MO, USA) solubilized in a 0.5 M NaOH solution and filtered through a 0.2 μm filter for sterilization (Minisart 0.2 μm syringe filter ...
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bioRxiv - Microbiology 2024Quote: ... a collagen raft mixture was prepared on ice via combining 3 mg/ml collagen (Millipore-Sigma, #08-115) with 10X E-Media (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... a collagen raft mixture was prepared on ice via combining 3 mg/ml collagen (Millipore-Sigma, #08-115) with 10X E-Media (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 10 μM of the agonist N-[(3R)-1-Azabiciclo[2.2.2]oct-3-yl]furo[2,3-c]pyridine-5-carboxamide (PHA-543613, from Sigma, PZ0135) for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: PESTANAL® analytical grade tefluthrin (2,3,5,6-tetrafluoro-4-methylbenzyl 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylic acid) was purchased from Sigma Aldrich. Tefluthrin stock solutions were prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2024Quote: 1.5 x 106 YTS NK cells were cultured in 3 mL of media containing 1.8 mL of previously obtained viral media and 8μg/mL polybrene (Sigma). The culture plates were spun for 2 hours at 400 x g and then placed in an incubator overnight at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Stained zebrafish larvae were anesthetized with 0.016% (m/v) Ethyl 3-aminobenzoate methanesulfonate salt (Tricaine; Sigma-Aldrich, A5040) and embedded in 1.8% low melting point agarose (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... The number of viable cells was quantified using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; M2128, Sigma) assay at 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... corrected for saturation and normalized to an external reference (sodium 3-(trimethylsilyl) propionate-2,2,3,3-d4 (TSP; Sigma-Aldrich)) and to cell number (64).
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bioRxiv - Microbiology 2024Quote: ... After 24 h media/inhibitor was aspirated and replaced with 20 μl of 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) and incubated at 37°C in 5% CO2 for 3 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Bands were stained in 5-bromo-4-chloro-3-indolyl-phosphate and nitro blue tetrazolium solution (Sigma-Aldrich). The membrane image was acquired using a digital steel camera EOS M6 mark II with a lens EF16-35 mm F4L IS USM (Canon Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... slides were cleared in Xylene (2 × 3 min) and coverslipped with DPX mounting medium (Cat# 44581, Sigma-Aldrich). Sections were photographed with an epifluorescence microscope (Model BX51 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total of 150 µL of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone; Sigma-Aldrich) dissolved in 100% ethanol (Wako ...
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bioRxiv - Bioengineering 2024Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and collagenase type 1 was purchased from Sigma-Aldrich. RNA extraction kit (Iran) ...
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bioRxiv - Cell Biology 2024Quote: Treatments for the LC3 assay included 3 hours with mTOR inhibitor Torin1 (Merck-Millipore, final concentration 250 nM), Bafilomycin A1 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 min at RT and then permeabilized for 3 min with 0.2% Triton-X 100 (Millipore Sigma) Over-extracted cells were treated with 0.4% Triton-X 100 ...
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bioRxiv - Cancer Biology 2024Quote: ... the PDMS stamp is placed on a silanized coverslip pretreated with 2% (3-Trimethoxysil) propyl-methacrylate (Sigma #M6514)) ...
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bioRxiv - Genetics 2024Quote: Adult zebrafish (3 months post fertilization) were euthanized using a 25x tricaine solution (4000 mg tricaine powder (Sigma), 979 ml distilled water ...
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bioRxiv - Microbiology 2024Quote: ... the animal’s drinking water was replaced from days 3-8 with water containing 5% L-arabinose (Sigma Aldrich). InducTn-seq samples were collected 8 days post inoculation by plating fresh feces on solid LB containing 50 µg/ml kanamycin ...
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bioRxiv - Microbiology 2024Quote: ... They were washed three times with PBS and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich, A3294) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was applied to 3 kDa MWCO filters (Amicon Ultra-0.5 Centrifugal Filter; Merck Millipore, Darmstadt, Germany), and centrifuged at 14,000 × g for 45 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... They were maintained in a Petri dish containing embryo medium complemented with 3% Methylcellulose (Sigma-Aldrich, USA, #274429) to allow dorsal and lateral pictures of the whole body on glass microscope slides ...
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bioRxiv - Cancer Biology 2024Quote: ... 3-(2-Methyl-5-nitro-imidazol-1-yl)-N-(2,2,2-trichloro-1-phenylamino-ethyl)-propionamide) (Sigma-Aldrich SML1503). Drugs were diluted in culture media at treatment.
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bioRxiv - Cell Biology 2019Quote: ... Elastic stain kit (Verhoeff van Gieson) and Masson’s Trichrome kit (all from Sigma-Aldrich), each according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... Elastic stain kit (Verhoeff van Gieson) and Masson’s Trichrome kit (all from Sigma-Aldrich), each according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Developmental Biology 2022Quote: ... using 1:4 phosphate buffer from the Total SOD kit (Sigma-Aldrich, kit 19160) for SOD activity ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HMGB1 levels were measured using a commercial kit (HMGB1 ELISA kit A76696, Sigma-Aldrich). The assay was performed as described in the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... BSA and lysozyme were quantified with a quantification kit (Rapid-kit, Sigma-Aldrich, UK) using GloMax Discover microplate reader (Promega) ...
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bioRxiv - Microbiology 2023Quote: A colorimetric assay kit (Phenolic Compounds Assay Kit MAK365, Sigma-Aldrich, St. Louis, USA) determined the total phenol content ...
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bioRxiv - Genetics 2021Quote: ... Then human 293T cells were transduced with lentiviral particles of plkoSCR (Mission plko.1 puro; SHC002) or shCNBP human (Mission plko.1 puro TRCN0000311158, SIGMA-ALDRICH) at a MOI=5 for 72h ...