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Citations for Millipore Sigma :
7651 - 7700 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Mitochondrial pellets were lysed in PBS with 1% n-dodecyl-β-D-maltoside (DDM; Sigma-Aldrich) and 1X protease-phosphatase inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... larvae used in live-imaging experiments were treated with E3 + 0.2 mM N-phenylthiourea (PTU, Sigma) beginning at 1 day post fertilization (dpf) ...
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bioRxiv - Microbiology 2023Quote: ... The glucose in HMM was replaced with equimolar amounts of N-acetyl-glucosamine (A3286, Sigma Aldrich) in indicated experiments ...
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bioRxiv - Microbiology 2023Quote: ... Samples were esterified with 200 µL of 0.5 N methanolic HCl (Sigma Aldrich, St. Louis, MO) at 65 °C for 1.5 hours with occasional vortexing ...
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bioRxiv - Physiology 2023Quote: ... HPLC-grade water and N-(1-Naphthyl)ethylenediamine dihydrochloride (NEDC) matrix were obtained from Sigma-Aldrich. Slides were prepared similar as previously described (21) ...
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bioRxiv - Developmental Biology 2023Quote: ... Ten (10) μL of injection standard (N-pentadecane, 100 μg/mL; 629-62-9, Sigma-Aldrich) were added and the samples were split into 2 vials for targeted and untargeted analysis.
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bioRxiv - Cell Biology 2022Quote: ... 20µl of room-temperature N,O-bis(trimetylsilyl)trifluoroacetamide (BSTFA) and 1% trimethylchlorosilane (TMCS) (Sigma Aldrich) was added to each sample ...
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bioRxiv - Cell Biology 2023Quote: ... and chromatin labeling using conjugated H3K27me3 (Origene, TA347154 conjugated using mix-n-stain CF555 kit, Sigma), H3K4me3 (C15410003 ...
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bioRxiv - Cancer Biology 2022Quote: ... Embryos were maintained at 28 °C and incubated with PTU 0,003% weight/volume (N-Phenylthiourea, Sigma) in E3 medium ...
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bioRxiv - Developmental Biology 2023Quote: ... the culture medium was also supplemented with 0.003% (w/v) N-phenylthiourea (PTU, Sigma-Aldrich #P7629). The IACUC committee at the University of Wyoming approved all animal procedures.
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bioRxiv - Genetics 2023Quote: ... as described previously,21 in a total volume of 100µL 50mM N-ethylmaleimide (Sigma, cat.no.04259) and 50µL of 12% sulfosalicylic acid (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... solution (50mM sodium phosphate, 2mM N-acetyl cysteine, 2mM EDTA, all from Sigma-Aldrich, pH 6.5) for 16-18h ...
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bioRxiv - Microbiology 2023Quote: ... D30 and D53 were soaked before harvest with N-Acetyl Neuraminic Acid (Sigma-Aldrich, Gillingham, UK) at a concentration of 10mM.
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bioRxiv - Molecular Biology 2023Quote: ... coli N-terminal domain (NTD; residues 1-130) of ProQ were cloned into pET15b vector (Novagen), as described (Stein et al ...
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bioRxiv - Cancer Biology 2023Quote: ... and indicated cell lines were enriched with 2 mM N-Acetylcysteine (Sigma-Aldrich, 616-91-1).
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bioRxiv - Immunology 2023Quote: The purified libraries were indexed by PCR using Extract-N-Amp PCR ReadyMix 2x (Sigma, E3004) and sample-specific i5 primers (10x genomics PN2000095 ...
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bioRxiv - Cancer Biology 2023Quote: ... For SK-N-SH assays compounds were administered at the following concentrations: 0.107 uM doxorubicin (Sigma), 3.18 uM cisplatin (Cambridge Bioscience) ...
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bioRxiv - Bioengineering 2024Quote: ... This gelatin stock was then adjusted to pH 7.4 using 1 N sodium hydroxide (Sigma-Aldrich). The gelatin was then sterile filtered and stored at 4 °C for up to 6 months ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted using the RED Extract-N-Amp Tissue PCR Kit (Sigma, St. Louis, MO) according to the protocol of Shazib et al ...
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bioRxiv - Bioengineering 2024Quote: ... and pancreatin from porcine pancreas 8 x USP (P/N: 7545) were purchased from Sigma Aldrich Inc. ...
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bioRxiv - Plant Biology 2019Quote: ... Transgenic BIN2-YFP-TbID and YFP-TbID seedlings were grown on 14N MS medium (1/2 MS without nitrogen source [PhytoTechnology Laboratories], NH4NO3 [0.5 g/L, Sigma], KNO3 [0.5 g/L, Sigma], pH 5.7) or 15N MS medium (1/2 MS without nitrogen source [PhytoTechnology Laboratories] ...
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bioRxiv - Cell Biology 2020Quote: ... DPSCs were cultured in the mineralization-inducing fluid (5% FBS, 10 mmol/L β-sodium glycerol phosphate, Sigma; 10−7mol/L dexamethasone, Sigma; 50 ng/mL ascorbic acid, Sigma) containing 0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cordycepin was added to the 3’ end of in vitro transcribed mRNA by substituting 50 mM Cordycepin (3’-deoxyadenosine) 5’-triphosphate sodium salt (Sigma-Aldrich) in 10 mM Tris pH7 for ATP in a poly(A)-tailing reaction with a Poly(A ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and blocked for 1-hr with 3% Normal Bovine serum in TBS containing 3% triton-X-100 (TBST, CAS-9002-93-1, Sigma, USA). Following blocking ...
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bioRxiv - Cancer Biology 2019Quote: ... The membrane was UV-crosslinked for 3 minutes/each side and hybridized with probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) and developed as described62 ...
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bioRxiv - Neuroscience 2019Quote: ... ten times into red fluorescent dye (3 mg Di-I [1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate] per 100 μL acetone; Sigma-Aldrich, St. Louis, MO). The probe was inserted several hundred microns into the cortex at each of the four designated points and left in place for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... The CGCaP composites were then crosslinked via carbodiimide chemistry in a PBS solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Sigma-Aldrich) and N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Neuroscience 2020Quote: ... the eluate was washed 3 times with PBS in an Amicon Ultra-4 Centrifugal Filter Unit (NMWL 3 kDa, Millipore, UFC800324) and finally concentrated to < 250 µl ...
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bioRxiv - Bioengineering 2020Quote: ... The film crosslinking level was controlled by incubating the coated scaffolds in 30 or 70 mg/mL 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma, France). The film crosslinking level has an effect on the film stiffness as shown in previous studies [23] ...
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bioRxiv - Bioengineering 2020Quote: Crosslinking of scaffolds were prepared by rehydrating scaffolds in PBS overnight with 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDAC, Sigma-Aldrich) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Cancer Biology 2020Quote: ... PRL-3 over expressing construct was made by cloning full length PRL-3 cDNA into p3XFLAG-CMV-14 expression vector (Sigma, E7908)
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Immunology 2022Quote: ... Cells were exposed to LPS (100 ng/mL) for 3 hr with ethanol or HNE (0.3-3 µM, Millipore Sigma, 393204) in appropriate growth media ...
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Biophysics 2021Quote: ... was performed using a pre-annealed DNA-RNA template (DNA45: 5’-TTCTTT ATCTCTTATTTCCTTCTATTTTCCACGCGCCTTCTATTT-3’; RNA13: 5’-[6FAM]-GGCGCGUGGAAAA-3’, Sigma Aldrich). The reaction buffer comprised 25 mM HEPES pH 7.2 ...
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bioRxiv - Microbiology 2022Quote: ... and 20 uL of 120 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (Pierce; dissolved in 47% ACN, 47% water and 6% HPLC-grade pyridine (Sigma Aldrich)) were added ...
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bioRxiv - Synthetic Biology 2020Quote: ... indole-3-propionic acid (order no. 57400) and indole-3-butyric acid (order no. i5386) have been obtained from Sigma-Aldrich. All ligands have been dissolved in 50mM Tris / 300mM NaCl pH8 buffer with if necessary 1% dimethyl-sulfoxide (DMSO) ...
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bioRxiv - Neuroscience 2019Quote: ... KO2 along with Cas9 donor plasmid were used as template DNA and amplified with F 5’-GAAGAAGAATGGCCTGTTCG-3’ and R 5’-GCCTTATCCAGTTCGCTCAG-3’ with KOD Xtreme (Novagen, #71975). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were then chemically cross-linked to the membrane during 90 min at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma Aldrich). Membranes were prehybridized for 30 min in PerfectHyb™ plus (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... and Grace Bio-Labs reusable CultureWellTM gaskets (3 mm x 1 mm, well capacity 3-10 ul, Sigma-Aldrich, GBL103250-10EA) were cleaned 3 times sequentially in 100% isopropanol and Milli-Q H2O ...
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bioRxiv - Developmental Biology 2022Quote: ... Medium was refreshed 3 days after seeding and 3 days later cells were fixed with citrate/acetone/3% formaldehyde and stained with Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... in 0,5× TBE buffer for 1h at 350 mA of current and EDC-cross-linked for 45 min at 60°C with a solution containing 33 mg/ml of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Sigma Aldrich), 11 ng/ul of 1-methylimidazol and 0.46% of HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... 53 from the RNAi Consortium (clones TRCN0000087290 and TRCN0000087292 with target sequence 5’-GCGGGAGTATAGTGAGTTTAA-3’ and 5’-CGGACAGTTTGGCACAATCAA-3’, respectively, distributed by Sigma-Aldrich, Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (Supplementary Table 1) in 3% dried milk (Marvel) or 3% BSA (Sigma Aldrich) at room temperature for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... DHEA sulfate (5-androsten-3β-ol-17-one-3-sulfate) and testosterone (4-androsten-17β-ol-3-one) were purchased from Sigma Aldrich. 11β-hydroxyandrostenedione (11β-hydroxy-4-androstene-3,17-dione) ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 10 min 100% EtOH) and cleared (3 x 5 min) in xylene (247642, CAS: 1330-20-7, Sigma Aldrich). Finally ...
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bioRxiv - Physiology 2023Quote: Tissue from the LV of 3 sedentary and 3 exercised mice of each sex was fixed in RNAlaterTM (Millipore Sigma, MFCD03453003), and RNA sequencing was performed by Genewiz from Azenta Life Sciences ...