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Citations for Millipore Sigma :
7651 - 7700 of 10000+ citations for 192 IgG Mouse Monoclonal Cy3 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Then the sections were incubated overnight at 4°C in 0.1% PBST containing the monoclonal antibody NeuN (Sigma-Aldrich, 1:1500). After three washes in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... 15-fold dilution of the result sample was loaded and resolved in 12% SDS-PAGE followed by immunoblot analysis anti-polyHIS monoclonal antibodies (Sigma, H1020). Scale-up experiments were done in order to purify His:SAE2UFDCt and perform subsequent MS analysis ...
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bioRxiv - Microbiology 2021Quote: ... Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich, 1:3000 dilution in 2% Milk in 1X TBS + 1% Tween-20) ...
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bioRxiv - Developmental Biology 2019Quote: ... H9 hESCs were nucleofected using the Lonza P3 Primary Cell 4D-Nucleofector X Kit and monoclonal colonies were selected for 7-10 days with 1 μg/ml of puromycin (Sigma-Aldrich). Tetracycline hydrochloride (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated with the rabbit anti-NeuN (1:800, Beyotime Biotechnology) or monoclonal anti-Dicer 1(1:300, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the plates were washed and incubated with an anti-IpaA (dilution 1/2000e) polyclonal primary antibody3 or anti-vinculin (dilution 1/2000e) Vin11Vin.5 monoclonal antibody (Sigma-Aldrich) in binding buffer containing 0.2% BSA for one hour at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Detection of FLAG-tagged proteins transferred onto a PVDF membrane was performed with monoclonal anti-FLAG M2-peroxidase (HRP) antibodies (Sigma-Aldrich) and Lumi-Light Western Blotting Substrate Kit (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... berghei ANKA 2.34 infected blood containing asexual parasite and gametocyte stages with a parasitaemia of 5-6% and gametocytaemia of 2-3% was mixed with 150 μL of PBS containing either α-Pbc43 or the isotopic monoclonal UPC10 (negative control) (Sigma) antibodies to yield final antibody concentrations of 50 ...
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bioRxiv - Microbiology 2021Quote: ... Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich, 1:3000 dilution in 2% Milk in 1X TBS + 1% Tween-20 ...
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bioRxiv - Microbiology 2021Quote: ... permeabilized with 0.2% Triton X-100 (in Phosphate Buffered Saline [PBS]) and subjected to an immunofluorescence assay (IFA) using a monoclonal antibody (MAb) anti-ACE2 (Sigma-Aldrich), and then incubated with a goat anti-rabbit IgG (goat anti-rabbit IgG ...
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bioRxiv - Microbiology 2021Quote: ... Protein complexes were isolated from the post nuclear fraction of the NP-40-lysed cells using magnetic beads loaded with Flag-specific monoclonal antibody (MAb) (Sigma, Mo) as described elsewhere (17) ...
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bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight at 4°C with a 1:5000 dilution of monoclonal rabbit anti-HA antibodies (Sigma-Aldrich) or 1:5000 dilution of monoclonal rabbit anti-FLAG antibodies (Sigma-Aldrich).
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bioRxiv - Genomics 2020Quote: ... Incubation with monoclonal anti-ATPIF1 (ATP synthase subunit IF1) antibody diluted in staining buffer (PBS with 1% bovine serum albumin (BSA) (Sigma-Aldrich)) was performed overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of lysate proteins were incubated with 2 µl of anti-Flag monoclonal antibody pre-adsorbed on protein A-Sepharose beads (cat. no. A2220, Sigma-Aldrich) overnight at 4°C.
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bioRxiv - Immunology 2022Quote: ... the anti-SOSV mix was diluted to 1:250 in permeabilization buffer while monoclonal anti-FLAG M2 (Sigma-Aldrich, cat. # F3165) was diluted 1:500 in permeabilization buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... the accumulated lysates were incubated with protein A/G magnetic beads (Thermo-Fisher) pre-coupled with anti-Flag antibody (M2 monoclonal antibody, Sigma-Aldrich) for 2 h at 4°C with rotation ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 1X TBST containing 5% milk and probed with a 1:2,000 dilution of monoclonal α-FLAG-HRP (Sigma-Aldrich), 1:5,000 dilution of E ...
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bioRxiv - Microbiology 2023Quote: ... after blocking with BSA and skim milk as described in (Basu and Altuvia 2021) and probed with FLAG M2 monoclonal antibody (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour and incubated overnight at 4°C with primary antibodies to label cells containing tyrosine hydroxylase (anti-tyrosine hydroxylase monoclonal, 1:1000 Millipore, MAB318) and mCherry (anti-dsRed polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation of nicks were carried out according to standard ChIP protocols using Protein G-coupled Dynabeads coated with monoclonal anti-biotin antibodies (Sigma-Aldrich) antibody ...
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bioRxiv - Microbiology 2023Quote: ... Cells were treated 1 h prior to infection with a 1:50 dilution of monoclonal antibody 1987 clone P4C10 (EMD Millipore) directed against β1 integrins.
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated in PBS supplemented with the pan-flavivirus anti-Env monoclonal antibody 4G2 (NovusBio, dilution 1:1000) at the presence of 0.05% Saponin (Sigma-Aldrich, France) for 1h ...
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bioRxiv - Neuroscience 2023Quote: Cytoplasmic and nuclear fractions were then subjected to western blotting and labelled with rabbit monoclonal anti-GAPDH (Sigma, G9545, 1:5,000) antibody and rabbit polyclonal anti-LSD1 antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... The upper part was used for protein detection using a specific monoclonal antibody against Cas9 (1:1000 dilution, #MAC133, Sigma-Aldrich) and Alexa555 secondary antibody for fluorescence detection (iBright ...
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bioRxiv - Physiology 2023Quote: ... blots were stripped of connexin or ZO-1 antibodies and reprobed with an actin-specific monoclonal antibody (Ω-actin, 1:5000, Sigma). Intensities of Cx43 and ZO-1 bands were normalized to those of actin and quantified using the Las-3000 software.
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with anti-Sulf-1/2 primary antibody (anti-Sulf-1: rabbit polyclonal, Sigma, SAB1410410, anti-Sulf-2: rabbit monoclonal clone 2B4, Sigma, MABC584) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein loading was determined using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000) from Sigma-Aldrich (A3854). Quantitation of western blot band intensity was done using Image J software.
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bioRxiv - Neuroscience 2024Quote: ... A rat anti-D1DR monoclonal antibody was used for D1DR-receptor staining (1:500; Sigma-Aldrich, St Louis, MO; Catalog #D2944). To enhance the GFP signals ...
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bioRxiv - Cell Biology 2024Quote: ... The axons were labeled with a monoclonal antibody against neuron-specific class III p-tubulin (clone Tuj-1 Babco) and the nuclei with DAPI (Sigma Aldrich). Pictures of explant were taken using a Leica fluorescent microscope and a Photometrics Coolsnap fx monochrome CCD camera (Roper Scientific ...
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bioRxiv - Microbiology 2024Quote: ... with levels for each clone determined by immunoblotting using a blend of monoclonal antibodies for the detection of VP1 (MAB8566, Sigma-Aldrich). For VLP production ...
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bioRxiv - Microbiology 2024Quote: ... Gradients were fractionated by manual collection from the top and fractions containing VLPs were identified by western blot analysis using a blend of monoclonal antibodies for the detection of VP1 (MAB8566, Sigma-Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... and incubated overnight with a mouse IgG2b monoclonal antibody (clone HUTS-4) directed against active β1 integrin and α-smooth muscle actin (clone 1A4, FITC-conjugated; Sigma), followed by 1 hour incubation with anti-mouse IgG2b Alexa-647 ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was probed with the indicated primary antibody (rabbit anti-GFP [1:1500, Abcam, #ab290], mouse anti-RFP [1:1000, Chromotek, #6G6], and mouse anti-2A [1:1000, Sigma, #3H4]), washed with TBST ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with anti-mouse NeuN or anti-mouse parvalbumin (Millipore, MAB377, 1:100 and Sigma, P3088, 1:1000 respectively) overnight at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were blocked in Odyssey blocking buffer for 30 min at room temperature and incubated with primary antibodies (Mouse anti-V5 antibody, Invitrogen R960-25, 1:1000 dilution; Mouse anti-Flag antibody (Sigma F1804), 1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... IF 1:500), mouse anti-cortactin (05-180, IF 1:400), mouse anti-talin (T3287, IF 1:200) all from Sigma-Aldrich; rabbit anti-EEA1 (ALX-210-239 ...
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bioRxiv - Biochemistry 2022Quote: ... mouse anti-GBP130 (1:500), mouse anti-EXP2 (1:5000, 37), rabbit anti-Nluc (1:400, 65) and mouse anti-HA (1:500, Sigma-Aldrich). After primary antibody was removed ...
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bioRxiv - Biophysics 2022Quote: ... The α-tubulin antibody was revealed using Abberior STAR Red goat anti-mouse (Abberior) for the custom microscope or Alexa 546 goat anti-mouse (Sigma Aldrich) for the Nikon-based microscope ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-βactin (AC-15, 1:20000) and mouse anti-FLAG (F-3165, 1:7000; IF 1:300) from Sigma-Aldrich; rabbit anti-TOP1 (A302-589A,1:5000) ...
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bioRxiv - Neuroscience 2023Quote: ... which were blocked in 5% dry milk powder in TBS-T for 1 hour and then incubated in primary antibody solutions (Rabbit anti NUDT6, Proteintech 1:800; mouse anti FGF2, Millipore, 1:500; mouse anti ß-actin, Sigma, 1:7500 ...
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bioRxiv - Immunology 2023Quote: ... Antibody-stained cells were subsequently probed with Duolink In Situ PLA Probe anti-mouse PLUS and anti-mouse MINUS probes (Sigma/Merck). Next ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was then pre-cleared for 1 h with 25 μL of IgG agarose beads (Sigma) at 4°C with constant rotation ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
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bioRxiv - Cell Biology 2020Quote: ... For microinjection, we used affinity-purified rabbit anti-GM130 (Wei et al., 2015) and rabbit IgG (Sigma).
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bioRxiv - Developmental Biology 2021Quote: ... 150 µL of 1% BSA-PBS containing 1:200 anti-rabbit FITC-IgG secondary antibody (Sigma, F6258) were added and incubated 1-2 h at RT in the dark ...
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bioRxiv - Immunology 2021Quote: ... Plasma samples from four Danish non-pregnant women without malaria exposure and purified human IgG (Sigma-Aldrich) were included as negative controls.
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bioRxiv - Molecular Biology 2020Quote: ... This step is repeated substituting IgG for the Anto-pan Ago antibody (clone 2A8; Millipore, Burlington, MA) (4 μl) ...