Labshake search
Citations for Millipore Sigma :
7551 - 7600 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... and incubated for 2.5 h at 37°C and 5% CO2 in 2% FBS DMEM supplemented with 100 µg ml-1 cycloheximide (#C4859, Sigma-Aldrich) to prevent viral proteins synthesis ...
-
bioRxiv - Biochemistry 2023Quote: ... 50 µL of the culture was spread onto pre-warmed plates containing 100 µg/mL ampicillin (Sigma-Aldrich, St. Louis, MO) for antibiotic selection and incubated at 37 °C overnight ...
-
bioRxiv - Immunology 2023Quote: ... injected into the left and right flank with 100 μl of an emulsion of MOG35-55 peptide (500 μg/ml) (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (made from incomplete Freund’s adjuvant (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with buffer H containing 0.1 M NaCl and 100 μg/mL of FLAG peptide (Sigma Aldrich, #F3290). Peak fractions were pooled and loaded onto a MonoQ 5/50GL column (Cytiva ...
-
bioRxiv - Microbiology 2023Quote: ... coli strains were grown at 37 °C in Luria-Bertani broth (LB, Difco) or on LB agar (Difco) supplemented with ampicillin (100 μg/mL, Sigma-Aldrich) when required.
-
bioRxiv - Biochemistry 2023Quote: ... 100 mM NaCl pH 7.3 and concentrated to 22 mg/mL using Amicon Ultra-4 centrifugal filters (Millipore, Billerica MA, USA).
-
bioRxiv - Neuroscience 2023Quote: ... The nodose ganglia were then transferred to a well containing 1 ml HBSS- and 100 µl Trypsin (Sigma; T9935-50 mg) and were incubated 5 min at 37°C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... 100 µl of the resultant culture was plated on both LB agar plates and LB agar plates supplemented with 100 µg/ml rifampicin (Sigma–Aldrich), with appropriate dilutions ...
-
bioRxiv - Immunology 2023Quote: ... Intracellular cytokine production was analyzed using flow cytometry after 4 hours of polyclonal restimulation with 100 ng/mL phorbol myristate acetate (PMA, P8139, Sigma-Aldrich) and 1 µg/mL ionomycin (I0634 ...
-
bioRxiv - Molecular Biology 2023Quote: U2OS were synchronized in late G2 and damaged locally with the UVC lamp as described above prior to release in metaphase in medium containing 100 ng/ml Colcemid (Sigma-Aldrich) for 2h at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.75 IU/kg of human insulin (Actrapid 100 IU/mL, Novo Nordisk) for insulin tolerance tests (ITT) or 2 g/kg pyruvate (Sigma-Aldrich) for pyruvate tolerance tests (PTT ...
-
bioRxiv - Immunology 2023Quote: ... Cells were cultured for 5 days at 37°C in a humidified 5% CO2 atmosphere under continuous high-dose 100 ng/mL lipopolysaccharide (LPS; Sigma- Aldrich) stimulation or PBS control conditions ...
-
bioRxiv - Bioengineering 2023Quote: ... LL37-SM hydrogels were prepared as 100 μL discs and submerged in 1 mL of phosphate buffer saline (PBS, Sigma-Aldrich) and incubated at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... each fraction was digested at 37°C for 90 min in a mix of proteinase K (final concentration 100 μg/ml; Sigma-Aldrich) and 1% SDS ...
-
bioRxiv - Cell Biology 2022Quote: ... These cells at a final confluency reached 25% and 100% when they were activated with LPS at 1 μg/ml (Sigma # L2630) for 6h ...
-
bioRxiv - Cell Biology 2022Quote: ... ME cells were briefly washed with DMEM/F12 and cultured with CDM2 supplemented with 100 ng/ml ActivinA and 250 nM LDN193189 (SML0559-5MG, Sigma-Aldrich) for 24 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... COC was transferred into a fresh 100 μl drop of M2 medium with hyaluronidase (concentration 0,1 mg /ml) (hyase, from bovine testes, H4272, Sigma-Aldrich®), covered with high viscous paraffin oil (P14501 ...
-
bioRxiv - Biochemistry 2023Quote: ... 1% sodium dodecyl sulfate (SDS), 1% Triton X-100 (Muinao et al., 2018)) with 5 μL/mL of protease inhibitor mixture (Sigma-Aldrich), and 1 μL/mL Benzonase Nuclease (250 U/μL ...
-
bioRxiv - Biophysics 2023Quote: ... SA-β-galactosidase activity: Cells were seeded on glass coverslips (Lab-tek, SPL Life Sciences) coated with 100 μg/mL fibronectin (Sigma-Aldrich) and β-galactosidase activity was measured following the manufacturer’s instructions (Senescence β-Galactosidase Staining Kit ...
-
bioRxiv - Biochemistry 2023Quote: ... The cells were resuspended in 500 μL sterile water and each replicate was plated on a BioAssay dish (245mm × 245mm) containing SC+leucine+methionine+histidine medium with 100 μg/mL methotrexate (Sigma-Aldrich) and 1 mM sulfanilamide (Sigma-Aldrich) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... cells and expressed in liter amounts in 2xYT media (FORMEDIUM, UK) in the presence of 100 μg/mL of ampicillin (SIGMA Co). Cells were grown at 37°C to an OD600 of 0.6 ...
-
bioRxiv - Cell Biology 2023Quote: ... the liquid culture phase was collected as a starter culture and diluted 1:100 in fresh BSFB (final volume of 2 L) supplemented with 5 µg/mL vancomycin (Sigma-Aldrich), and incubated on a shaker platform in a humidified ...
-
bioRxiv - Neuroscience 2023Quote: ... of the rostral-dorsal striatal wall in newborn male and female mice was dissected out and mechanically dissociated in DMEM/F-12 containing 100 units/mL penicillin‒streptomycin (P0781, Sigma‒Aldrich) to obtain NSCs ...
-
bioRxiv - Microbiology 2023Quote: ... Viruses were purified to remove viral culture fluid using Amicon® Ultra-0.5 ml centrifugal filters (100 kDa MWCO; Millipore UFC5100) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... and SKR-2-containing protein complexes were eluted overnight in 100 µl of the lysis buffer containing 2 mg/mL of 3xFlag peptide (Sigma F4799). Eluted protein samples were digested with trypsin and analyzed by MudPIT in the Mass Spectrometry and Proteomics Facility at Johns Hopkins School of Medicine.
-
bioRxiv - Immunology 2023Quote: ... Extracellular nitrite released from BMDMs (1×105 cells/well) stimulated with either HK-ST (MOI = 10) or 100 ng/ml LPS (Sigma, #L7895) was measured using a colorimetric assay based on the Griess reaction (Green et al. ...
-
Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... The blocking solution was made up fresh and for every mL of solution one adds 14.6 mg Malemide [100 mM](Sigma cat#129585) and 5.35 mg NH4Cl [100 mM](Sigma cat#A9434 ...
-
bioRxiv - Biochemistry 2023Quote: ... to ensure a minimum of 100x of the complexity of each library and only then the 100 mL cultures were midi prepped (Millipore Sigma) and the DNA concentration was assessed by NanoDrop spectrometer ND-1000.
-
bioRxiv - Neuroscience 2023Quote: ... and single cells seeded and grown as adherent cultures in flasks coated with 100 μg/ml Poly-L-ornithine (Sigma-Aldrich) and 5 μg/ml mouse laminin in media composed of advanced DMEM/F12 ...
-
bioRxiv - Neuroscience 2023Quote: ... samples were first permeabilized in PBS 0.5% Triton X-100 for 2h at room temperature and incubated in 10µg/mL DAPI (Sigma-Aldrich-Merck, D9542) for 30min ...
-
bioRxiv - Bioengineering 2023Quote: ... 1x106 cells per well were seeded in a 96-well round-bottom plate and stimulated with 100 μg/mL free OVA (Sigma-Aldrich) for 4 days ...
-
bioRxiv - Immunology 2023Quote: ... 3-4 and 7-9 were treated with 100 µL of embryo water plus 1 µg/mL 2′,7′-Dichlorofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) in 0.4% DMSO ...
-
bioRxiv - Microbiology 2023Quote: ... cells were seeded on coverslips in 12-well plate and challenged with 100 pg/ml LPS (Sigma, L4391; diluted in PBS) for 12 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... was diluted to 0.01 mg/mL concentration in 100 µL of 25 mM Tris/HCl buffer containing various concentrations of SAHS proteins or BSA (Sigma-Aldrich). 50 µL of each sample was stored at 4°C while the other 50 µL was dried using a Savant SPD131DDA SpeedVac (Thermo Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell pellets were lysed in 6 mL RIPA buffer (FUJIFILM) containing 1 mM EDTA and 1:100 protease inhibitor cocktail (Sigma-Aldrich). The lysate was rotated at 4°C for 30 min ...
-
bioRxiv - Systems Biology 2023Quote: ... Then the pellet was resuspended in 1 mL 100 mM sodium acetate (pH 4.5) supplemented with amyloglucosidase powder (Sigma-Aldrich, 10115) to a final concentration of 35 U/mL ...
-
bioRxiv - Molecular Biology 2024Quote: ... at 6-8 weeks of age via intraperitoneal injections (100 μL of 4 mg/mL solution per day for 4 consecutive days) dissolved in corn oil (Sigma, C8267). All mice were used in mixed genetic backgrounds ...
-
bioRxiv - Biophysics 2024Quote: ... NEDD4-2 HECT domain was subsequently isolated from the supernatant with anti-FLAG antibody (M2) affinity chromatography and eluted with 100 μg/mL FLAG peptide (Sigma Millipore) in 50 mM TrisHCl ...
-
bioRxiv - Neuroscience 2024Quote: ... myelinating cultures were treated for ≥ 5 minutes with either picrotoxin (PTX) (final concentration 100 μM; 1 μL stock solution in DMSO in 1 mL media; P1675; Sigma-Aldrich), tetrodotoxin (TTX ...
-
bioRxiv - Cancer Biology 2024Quote: ... M1 macrophage differentiation medium was prepared using M1 macrophage attachment medium supplemented with 100 ng/mL of LPS (Sigma-Aldrich L2630) and 50 ng/mL of IFN-γ (R&D Systems 285-IF).
-
bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 20 mL cold STE buffer (10 mM Tris-HCl [Sigma-Aldrich], pH 7.3, 100 mM NaCl [Sigma-Aldrich] ...
-
bioRxiv - Neuroscience 2024Quote: ... 1.8-2.0 mm lateral from the midline) and either ChABC (Sigma-Aldrich; C3667; 100 U/ml, in 0.1% BSA in PBS) or penicillinase (PEN ...
-
bioRxiv - Immunology 2024Quote: ... RRID:AB_10718401), rabbit polyclonal anti-GM130 (2ug/ml, Novus NBP2-53420G, RRID:AB_2890912) and rabbit anti-LAMP1 (1:100, Sigma-Aldrich L1418, RRID:AB_477157). Images were acquired using a Keyence BZ-X710 at 40X and 60X magnification ...
-
bioRxiv - Microbiology 2024Quote: ... Confirmed plasmids were midi-prepped from a 100 mL culture as described above and further concentrated using Novagen Pellet Paint Co-precipitant (Millipore Sigma).
-
bioRxiv - Immunology 2024Quote: ... respectively) were seeded in Seahorse XF 96 designated plates using 100 µg/ml poly-D-lysine (Sigma-Aldrich, A-003-E) and assayed according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... Trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2- propenylidene]malononitrile (DCTB) (100 mg mL-1 in ACN) was used as the matrix without further purification (Sigma-Aldrich). NaTFA salt was used as the ionization agent (1 mg mL-1 in MeOH) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Polarization of M0 macrophages for 48 h in macrophage medium supplemented with 100 ng/mL lipopolysaccharide (LPS; Escherichia coli O127:B8, Sigma Aldrich) and 20 ng/mL interferon-γ (Peprotech ...
-
bioRxiv - Systems Biology 2024Quote: ... and the gel pieces dried in a speedvac (miVac Duo concentrator, Genevac Ltd) before reduction with 100 mL 20mM DL-Dithiothreitol (DTT) (Sigma-Aldrich) in 100mM ABC for 60 min at 55 °C and alkylation with 200 mL 55mM iodacetamide (IAA ...
-
bioRxiv - Molecular Biology 2022Quote: ... 100 μM TCEP and 100 U salt active nuclease (Sigma-Aldrich) supplemented with protease inhibitor cocktail for 1.5 h incubation at room temperature (RT) ...
-
bioRxiv - Cell Biology 2024Quote: ... and 100 U·mL−1 penicillin/100 μg·mL−1 streptomycin (Sigma, 0781). Cells were washed five times with PBS and then ...