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Citations for Millipore Sigma :
7451 - 7500 of 10000+ citations for Rabbit Anti Human IgG+IgM+IgA Alexa Fluor 660 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Precipitations were also carried by IgG raised in same host (Merck Millipore) as the isotype control ...
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bioRxiv - Molecular Biology 2023Quote: ... WCEs were pre-cleared with Mouse IgG-Agarose resin (Sigma-Aldrich #A0919) for 2 hours at 4 °C and then incubated with anti-Flag M2 affinity gel (Millipore Sigma # A2220 ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG Merck #F3165) and suitable IgG (from Millipore, for Isotype control or Mock) were used to perform the ChIP assays as per protocol reported in Mukherjee et al ...
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bioRxiv - Immunology 2022Quote: Cells were mounted with Fluor-mount (Sigma-Aldrich, St Louis, MO, USA) and images were acquired through confocal microscope LSM 800 ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation in a solution containing 20 µM Azide fluor (Sigma), 2 mM CuSO4 (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... FBXW7 rabbit monoclonal (SP-237) (Spring Bioscience, CA) and the USP28 rabbit polyclonal (HPA006778) (Sigma Aldrich) antibody staining were performed using the Ventana Bench Mark ULTRA™ automated staining platform and the Optiview™ Detection kit (12) ...
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bioRxiv - Cell Biology 2020Quote: ... Western blots were probed using the following primary and secondary antibodies (incubated in PBS, 0.1% Tween20 and 5% milk): rabbit anti-Eps8L2 (Sigma-Aldrich #HPA041143, 1:500), mouse anti-Flag (Sigma-Aldrich #F1804 ...
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bioRxiv - Plant Biology 2021Quote: ... and subjected to a one-step immuno-affinity reaction using the rabbit peroxidase-anti-peroxidase soluble complex antibody (Sigma-Aldrich, St. Louis, USA). The membrane was developed ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Zoology 2019Quote: ... then washed in PBS with 0,1% Triton X100 and incubated in the secondary anti-rabbit CF488 antibody (SAB4600044, Sigma-Aldrich, diluted 1:1000) for 8 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated overnight with primary antibodies (rabbit phospho-AURKA Thr288, 1:50; mouse anti-γ-tubulin GTU-88, Sigma-Aldrich; 1:1000) diluted in blocking buffer at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Live-labelling of surface CNIH was performed at 48 hours post-transfection by incubation with rabbit anti-HA (Sigma, Cat# H6908; 1:200) for 20 min at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... Dendrites were identified using a staining against the microtubule-associated-protein-2 with Rabbit-anti-MAP2 PAB (Milipore Sigma, cat. AB5622, 1:1000) and GAR-STAR488 SAB (Abberior ...
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bioRxiv - Microbiology 2021Quote: ... The blot was washed with TBST (4x 5 min) and incubated with a goat anti-rabbit alkaline phosphatase secondary antibody (Sigma-Aldrich, Steinheim, Germany) diluted 1:30,000 in TBST containing 3 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-FLAG rabbit polyclonal and mouse monoclonal (Cat# F7425, RRID: AB_439687 and Cat# F1804, RRID: AB_262044, respectively, Sigma-Aldrich, St. Louis, MO, USA), anti-GFP rabbit polyclonal (Cat# A-11122 ...
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bioRxiv - Genetics 2022Quote: ... in PBS and incubated with rabbit polyclonal vault antibodies (N2-B15, provided by Leonard Rome, dilution 1:1000) and mouse anti-NeuN antibodies (MAB377, Millipore, dilution 1:1000) in 0.1%NDS/PBS at 4 °C overnight under agitation ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were stained using primary antibodies for 3 h at RT followed by Duolink in situ PLA probes with anti-rabbit MINUS (Sigma-Aldrich, Cat. # DUO92005), anti-mouse PLUS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit conjugated to peroxidase (Perkin Elmer and Sigma, Aldrich respectively). The latter were developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences).
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bioRxiv - Pathology 2020Quote: ... Expression of human COMP in RCS cells was validated by Western Blot with anti-Flag antibody to recognize the tagged COMP (1:5000 F7425 rabbit polyclonal from Sigma, Saint Louis, MO). DOX was used for 4 days to induce COMP expression in RCS cells ...
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bioRxiv - Neuroscience 2020Quote: ... We incubated free-floating CeA coronal sections (16 µm) for 2 h at 30 °C with rabbit anti-FG antibody (Millipore; AB153I; 1:200) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Cell Biology 2021Quote: ... Human-αSMA staining was visualised by 1h incubation with a secondary goat anti-rabbit-HRP conjugated antibody followed by a 10 min incubation with 3,3’-diaminobenzidine (DAB Fast Tablet, Sigma-Aldrich, St. Louis, MO). Nuclear counterstaining was performed with hematoxylin after which the sections were dehydrated and mounted with Entellan.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked for one hour in 5% normal goat serum in PBST and incubated overnight with rabbit anti-p75NTR (Millipore 07-476, 1:1000) and phalloidin-Alexa Fluor® 488 (Invitrogen A12379 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was washed in TBST and incubated for 1h in TBST containing 4% dried milk and rabbit anti-MYO10 (1:300, Sigma-Aldrich Ref. HPA024223) as primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43 ...
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bioRxiv - Neuroscience 2023Quote: ... eYFP fluorescence was used to confirm TH+ expression in VTA cell bodies using a rabbit anti-TH antibody (1:1000; Millipore Sigma, Burlington, MA) as the primary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte cell bodies were identified by immunolabeling against Glutamine Synthetase using rabbit anti-Glutamine Synthetase antibody (1:500, Sigma-Aldrich catalog# G2781, RRID:AB_259853), and GABA immunoreactivity was detected by immunostaining with guinea pig anti-GABA antibody (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... A housekeeping protein was also detected using the same blot by mild stripping and re-probing the membrane with an anti-GAPDH rabbit polyclonal antibody (Sigma-Aldrich, G9545-200).
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bioRxiv - Neuroscience 2024Quote: ... Primaries were prepared in 10% normal goat serum and applied as combinations of either: 1) anti-Aβ1-40 rabbit polyclonal (Millipore Sigma #AB5074P, 1:200) and anti-Aβ1-42 12F4 mouse monoclonal (Biolegend #805502 ...
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bioRxiv - Neuroscience 2024Quote: ... sections from a subset of animals (n=16) were processed identically as described above with rabbit anti-nNOS (Millipore Cat# AB5380, RRID AB_91824) and the appropriate secondary antibody conjugated to Alexa Fluor 647 ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed 6 x 10 mins in PBSTB and incubated with secondary antibody overnight: 1:500 HRP-conjugated Goat anti-rabbit antibody (Millipore Cat# 12-348) followed by washes 6 x 10 with PBSTB and mounted in VECTASHIELD mounting media ...
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bioRxiv - Microbiology 2024Quote: ... Thereafter the cells were incubated at 4 °C overnight in blocking-solution containing the primary antibody (Rabbit anti-hemoglobin (SIGMA, Cat. No. H4890) diluted 1/1000) ...
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bioRxiv - Immunology 2023Quote: ... The membranes were washed with 1×TBST for three times followed by incubation with a streptavidin-horseradish peroxidase (1:2000, streptavidin-HRP) or an anti-rabbit antibody conjugated with HRP (1:2000, Sigma, Cat#12-348) at room temperature for 1 hr ...
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bioRxiv - Biophysics 2023Quote: ... Sections were cut (5 µm) and immunolabeled as described above using a rabbit polyclonal anti-Cx43 antibody (Sigma-Aldrich C6219, St. Louis, MO) followed by goat anti-rabbit Alexa 568 (1:4000 ...
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bioRxiv - Neuroscience 2023Quote: ... nonspecific binding sites were blocked and slices were incubated overnight with a rabbit anti-tyrosine hydroxylase (TH) (1:1000, Millipore Cat# AB152, RRID:AB_390204), a rat anti-DAT (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with 0.05% Tween-20 in phosphate buffered saline (PBST) for 3 × 5 min with agitation and incubated with anti-β actin (1:1000 rabbit polyclonal (Sigma Aldrich; A2066) or 1:5000 mouse monoclonal (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... slow developmental (S46, 1:50; Developmental Studies Hybridoma Bank; Iowa City, IA) and rabbit anti-NF-H (1:500; EMD Millipore; Burlington, MA) for 24 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and probed with the appropriate primary antibody diluted in TBS-T with 3% BSA: rabbit anti-HA (Sigma Aldrich #H6908; 1:3,000 dilution), mouse anti-FLAG (Sigma Aldrich #F1804 ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 1h in a blocking solution of 0.01 M PBS with 0.25% Triton-X and 3% normal goat serum (NGS) and then incubated overnight at 4 °C with rabbit anti-NeuN antibody (1:2000, MAB5504, Millipore, Temecula, CA, USA) in 0.01 M PBS with 0.25% Triton-X and 3% NGS ...
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bioRxiv - Physiology 2024Quote: ... and the membrane was incubated for 2h at RT with rabbit anti-GAPDH antibody (Sigma Aldrich, G9545, diluted 1:2000 in TBS-T) at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti-TH antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 44-48 hr ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti DsRed polyclonal antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 18-22 hr ...
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245, 1:1000; Rhodamine Phalloidin, Fisher Scientific 50-646-256, 1:200; DAPI, Sigma Aldrich 10236276001 ...
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bioRxiv - Bioengineering 2023Quote: ... Phalloidin (Biosciences Alexa 488, A12379) and DAPI (Sigma 32670-5MG-F) staining was completed for 20 minutes and 5 minutes respectively at a concentration of 1:40 and 1:2000.
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bioRxiv - Neuroscience 2024Quote: ... followed with Alexa 488/555/647-conjugated secondary antibodies (Sigma-Aldrich) for 2 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Rabbit α-SOX9 (EMD Millipore, #ab5535), Rabbit α-SOX2 (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... Sec61α (rabbit polyclonal, 1:1000, Sigma), Sec61β (rabbit polyclonal42 ...
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bioRxiv - Neuroscience 2019Quote: ... Calnexin (1:1000, rabbit, Sigma C4731) were diluted in the same solution and incubated overnight at 4 °C ...