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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Recombinant Human TPBG Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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bioRxiv - Plant Biology 2019Quote: ... using a VP-ITC microcalorimeter (MicroCal) as described [34] after extensive dialysis of the purified recombinant protein in Chelex 100 (Sigma) to remove contaminating divalent cations ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Cell Biology 2019Quote: ... Immune sera were generated by immunization of 6 weeks-old female NMRI mice (Charles Liver Laboratories) via subcutaneous injection of 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma-Aldrich) followed by a boost after 4 weeks with 50 µg of recombinant protein ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biophysics 2021Quote: Catalytic activity was evaluated in vitro using 20 pmol of purified recombinant proteins in a 200 µl reaction buffer supplemented with 20 mM p-nitrophenyl phosphate (Sigma) as substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with ESC FCS (Millipore Sigma), non-essential amino acids (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... supplemented with FCS (Sigma-Aldrich, UK) and penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% FCS (Sigma Aldrich) and 2mM L-Glutamine (Gibco ...
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bioRxiv - Microbiology 2019Quote: ... 10% fetal calf serum (FCS) (Sigma), and 1% Penicillin-Streptomycin (PS ...
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bioRxiv - Biochemistry 2020Quote: ... medium supplemented with 10% FCS (Sigma) and 1% penicillin/streptomycin (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... medium supplemented with 10% FCS (Sigma) and 1% penicillin/streptomycin (Sigma) ...
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bioRxiv - Biophysics 2020Quote: ... containing 10 % FCS (Sigma-Aldrich, #F7524), 100 U/ml penicillin and 0.1 mg/ml streptomycin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% FCS (Sigma Aldrich), 1X Glutamax (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% FCS (Sigma, #9665) and 1x NEAA (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 5% FCS (Sigma, #F4135), 1% penicillin (10,000 Units/ml ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (Sigma-Aldrich). At least 24 h before transfection ...
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bioRxiv - Immunology 2021Quote: ... FCS was obtained from Sigma-Aldrich. HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10 % FCS (Sigma-Aldrich), 100 U/mL Penicillin and 100 μg/mL Streptomycin (Gibco ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 15% FCS (Sigma, C8056), 100 U/ml penicillin-streptomycin (Cellgro ...
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bioRxiv - Neuroscience 2022Quote: ... 10% FCS (Sigma-Aldrich, Germany, EU), 2 mM L-Glutamine (Biochrom ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and 1% PenStrep (GIBCO) ...
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bioRxiv - Molecular Biology 2020Quote: ... in FC-40 (Sigma Aldrich, USA). The devices were incubated at 95 °C overnight and afterward stored at room temperature until further usage for droplet production.
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bioRxiv - Genomics 2021Quote: ... with 10% FCS (Sigma, Cat # F0926), with 100U/mL Pen-Strep (Gibco ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% FCS (Sigma Aldrich), L-glutamine (2◻mM ...
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bioRxiv - Immunology 2021Quote: ... 10% fetal calf serum (FCS) (Sigma), 0.1 U/ml penicillin (Lonza) ...
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bioRxiv - Neuroscience 2022Quote: ... 10% FCS (Sigma-Aldrich, Germany, EU), 2 mM L-Glutamine (Biochrom ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% FCS (Sigma-Aldrich), 2 mM GlutaMAX (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% FCS (Sigma-Aldrich), 2 mM GlutaMAX (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich), PenStrep (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FCS (Sigma, F2442), pen-strep ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% FCS (Sigma, F2442), GlutaMAX 1x (Gibco ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich), Pen-Strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich), PenStrep (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... containing 10% FCS (Sigma-Aldrich, #F7524), 100 U/mL penicillin and 0.1 mg/mL streptomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-Fc (M4280, Sigma Aldrich) 1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Cell Biology 2022Quote: ... and dextran tagged with fluorescein (40,000 MW, Sigma Aldrich) were resuspended in 100 μl HBSS (4 mg/ml ...
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bioRxiv - Developmental Biology 2019Quote: Biotin-tagged OSTN was labeled by Cy3-streptavidin (Sigma). Equal volume of 25 μM biotin-tagged OSTN and 0.1 mg/ml Cy3-streptavidin were incubated with on ice for 15 minutes at 4 C ...